• Title/Summary/Keyword: Sensitive Industry

Search Result 468, Processing Time 0.03 seconds

Production of recombinant nucleocapsid protein of Newcastle disease virus in Escherichia coli for a diagnostic ELISA

  • Kim, Hyun-Il;Park, Kyoung-Phil;Park, Chan-Hee;Cho, Hyun-Ah;Yang, Ho-Suk;Hahn, Tae-Wook
    • Korean Journal of Veterinary Research
    • /
    • v.49 no.1
    • /
    • pp.39-44
    • /
    • 2009
  • Transmission of avian viruses both bird-to-bird and from birds to non-avian species is a major health concern. Newcastle disease virus (NDV) is an economically important avian virus that poses substantial risks to the poultry industry. Rapid and sensitive diagnostic methods, such as the enzymelinked immunosorbent assay (ELISA), are required to track such infections. To develop an ELISA for detecting anti-NDV antibody in avian sera, the nucleocapsid protein (NCP) gene of the NDV La Sota strain was cloned and expressed in Escherichia coli and the 513-amino acid recombinant NCP was purified by Ni-NTA affinity chromatography. To evaluate its ability to replace NDV whole virus antigen as a coating antigen, NCP-coated and whole NDV-coated ELISAs were tested and compared using a panel of NDV positive antisera from chickens. Results using purified NCP were highly correlated with those obtained using whole NDV (r= 0.927), demonstrating that recombinant NCP expressed in Escherichia coli is a suitable substitute antigen for whole NDV in a diagnostic ELISA.

Stabilization Converter Design and Modeling of LEO Satellite Power Systems (저궤도 위성의 전력 시스템 안정화를 위한 모델링 및 제어)

  • Yun, Seok-Teak;Won, Young-Jin;Lee, Jin-Ho
    • Journal of Satellite, Information and Communications
    • /
    • v.5 no.2
    • /
    • pp.29-33
    • /
    • 2010
  • Satellites industry has been developing with the commercial and military needs. Because power system of satellite is very important to survival operation and hard to test, increasing reliability is very critical. Due to LEO small satellites are very sensitive to power system, effective stabilization control is important. Therefore, this paper introduce methods for general modeling of power converting system which it can be used design of controller and analysis of external disturbance influences. In conclusion, a modeling of LEO small satellites power converting system and a possible guide line to design reliable controller which optimizing power converters of LEO small satellite are generated.

Experimental Investigations on Explosion Characteristics of LPG/Air Mixture by Electrostatic Discharge Energies (정전기 방전에너지에 따른 LPG/공기 혼합물의 폭발특성에 관한 실험적 연구)

  • Kim, Nam-Suk;Park, Dal-Jae
    • Journal of the Korean Society of Safety
    • /
    • v.26 no.6
    • /
    • pp.26-30
    • /
    • 2011
  • Experimental investigations were performed to examine the effects of different electrostatic discharge ignition energies on LPG/air mixture explosions in an explosion chamber. The chamber consisted of 500 mm in length, with a $100{\times}100mm^2$ cross section. Three different ignition energies were used: 0.30 mJ, 46 mJ and 98 mJ. Flame propagations were recorded by a high speed video camera. The results of flame speed and pressure obtained from the different ignition energies were discussed. It was found that as the energy increased, different flame initiations occurred. This caused the time interval in both the flame and pressure developments. It was also found that the flame speed and the pressure were less sensitive to both 0.30 mJ and 46 mJ, except for the ignition energy of 98 mJ.

Detection of Explosive RDX using Parallel Plate Waveguide THz-TDS (평행판 도파관 THz 분광을 이용한 폭발물 RDX 검출)

  • Yoo, Byung Hwa;Chung, Dong Chul;Kang, Seung Beom;Kwak, Min Hwan;Kang, Gwang-Yong
    • The Transactions of The Korean Institute of Electrical Engineers
    • /
    • v.61 no.12
    • /
    • pp.1939-1943
    • /
    • 2012
  • In this paper we presented the detection of the explosive material RDX using a parallel plate waveguide (PPWG) THz time domain spectroscopy (TDS). Normally the explosive materials have been characterized through identification of vibrational fingerprint spectra. Until now, most of all THz spectroscopic measurements have been made using pellet samples where disorder effects contribute to line broadening such that individual resonances merge into relatively broad absorption features. In order to avoid such disadvantages we used the technique of PPWG THz-TDS to achieve sensitive characterization of explosive material RDX. The PPWG THz-TDS used in this work well established ultrafast optoelectronic techniques to generate and detect sub-picosecond THz pulses. The explosive material was analyzed as powder layers in $112{\mu}m$ gap of metal PPWG. The thin later mass was estimated to be about $700{\mu}g$. Finally, we showed spectra of explosives from 0.2 to 2.4 THz measured using PPWG THz-TDS.

Recent Trends of Advanced Biosensors for Mycotoxin Analysis

  • Shim, Won-Bo
    • 한국균학회소식:학술대회논문집
    • /
    • 2016.05a
    • /
    • pp.35-35
    • /
    • 2016
  • A mycotoxin is a toxic secondary metabolite produced by organisms of the fungus kingdom, commonly known as molds and has been widely contaminated in agricultural products such as grains and cereals. Many methods including high performance liquid chromatography (HPLC) and gas chromatography (GC) have already been proposed and reviewed for mycotoxins. These methods are either expensive or time-consuming due to the complication of sample preparation and pre-concentration before determination. In addition, both methods are unsuitable for the routine screening of large sample numbers. A biosensor is a fictive analytical device that combines a biological component with a physicochemical detector for the detection of an analyte. Biosensors represent a rapidly expanding field, at the present time, with an estimated 60% annual growth rate; the major impetus coming from the health-care industry but with some pressure from other areas, such as food safety and environmental monitoring. Antibodies and aptamers are bioreceptors which have been used in the development of biosensors. There are many kinds of antibodies and aptamers specific to mycotoxin, and antibody (or aptamer)-based biosensors have been successfully developed for the detection of mycotoxin. The biosensors permit the rapid, sensitive, simple, and on-site detection of a range of mycotoxins and can be an alternative method to traditional methods such as HPLC and GC. This presentation provides the development trends of biosensors to mycotoxins and their application to food and agricultural products.

  • PDF

A Study on Characteristics of the Electrochemical Corrosion of Weld Zone for Refrigerating and Heating Systems Pipe (냉난방용 배관 용접부의 전기화학적 부식특성에 관한 연구)

  • Lim, Uh-Joh;Yun, Byoung-Du;Kim, Hwan-Sik
    • Journal of Fisheries and Marine Sciences Education
    • /
    • v.19 no.1
    • /
    • pp.84-90
    • /
    • 2007
  • This paper was studied on the electrochemical corrosion characteristics of weld zone for refrigerating and heating systems pipe. Austenitic stainless steel is widely applied to various fields of industry, because it is good to corrosion resistance and mechanical properties. But STS 304 is reliable to sensitization by heat cycle on welding. Therefore, in this study, electrochemical polarization test of STS 304 steel pipe manufactured by arc welding in tap water was carried out. And then polarization resistance behavior, uniform and local corrosion behaviors of base metal(BM), weld metal(WM) and heat affected zone(HAZ) for STS 304 pipe were investigated. The corrosion current density of STS 304 steel pipe is high in order of BM(153nA/cm2) < WM(614nA/cm2) < HAZ ($1.675{\mu}A/cm2$). The pitting potential of HAZ(238mV/SCE) for STS 304 is lower than BM(1206mV/SCE) and WM(369mV/SCE). Therefore, the local corrosion like pitting corrosion, galvanic corrosion and crevice corrosion of HAZ for STS 304 is more sensitive than BM and WM.

The Suggestion for Improvement of the Education System of Private Security Employees (민간경비원 교육과정의 문제점과 개선방안)

  • Ahn, Hwang-Kwon;Kim, Il-Gon
    • Convergence Security Journal
    • /
    • v.12 no.2
    • /
    • pp.13-21
    • /
    • 2012
  • The purpose of this study is to profile actual conditions of personal information protection systems operated in overseas countries and examine major considerations of personal information that security service providers must know in the capacity of privacy information processor, so that it may contribute to preventing potential occurrence of any legal disputes in advance. Particularly, this study further seeks to describe fundamental idea and principle of said Personal Information Protection Act; enhancement of various safety measures (e.g. collection/use of privacy data, processing of sensitive information/personal ID information, and encryption of privacy information); restrictions on installation/operation of video data processing devices; and penal regulations as a means of countermeasure against leakage of personal information, while proposing possible solutions to cope with these matters. Using cases among foreign countries for this study.

Density Measurement Comparisons of Specific Gravity Meter and Gas Chromatography in the Field (실제조건에서 기준 밀도계와 가스 분석기에 의한 밀도 측정 결과 비교)

  • Lee, Kang-Jin;Her, Jae-Young;Ha, Young-Cheol
    • 유체기계공업학회:학술대회논문집
    • /
    • 1999.12a
    • /
    • pp.90-96
    • /
    • 1999
  • In contracts for sales of natural gas between sellers and buyers, it is not suncient to only measure a volumetric quantity of gas in flowing conditions in metering station. Therefore the measured volumetric quantity must be converted to that of reference conditions. The density of the natural gas required in such a calculation can be measured directly or estimated from the equation of sate or any other experimental methods. The specific gravity meter is the apparatus used to measure the density of fluids under the reference conditions and it can be widely used in industrial areas, especially in massive flow rate natural gas industry. This study has been carried out in an attempt to improve measurement accuracy of natural gas flow rate calculation, providing the adequate installation and proper operation conditions of specific gravity meter. The test results are 1) the density measurement errors in case of using methane and standard gas as calibration gases are smaller than using methane and nitrogen gas, 2) the periodical calibration to maintain accurate density measurements is essential, and 3) the specific gravity meter is sensitive to changes of environmental conditions, especially environmental temperature surrounding the specific gravity meter.

  • PDF

Improvement of PLL Method for Voltage Control of Dynamic Voltage Restorer (동적전압보상기의 전압제어를 위한 PLL 방식의 개선)

  • Kim, Byong-Seob;Choi, Jong-Woo
    • The Transactions of The Korean Institute of Electrical Engineers
    • /
    • v.58 no.5
    • /
    • pp.936-943
    • /
    • 2009
  • Dynamic voltage restorer(DVR) is now more preferable enhancement than other power quality enhancement in industry to reduce the impact of voltage faults, especially voltage sags to sensitive loads. The main controllers for DVR consists of PLL(phase locked loop), compensation voltage calculator and voltage compensator. PLL detects the voltage faults and phase. Compensation voltage calculator calculates the reference voltage from the source voltage and phase. With calculated compensation voltage from PLL, voltage compensator restores the source voltage. If PLL detect ideal phase, compensation voltage calculator calculates ideal compensation voltage. Therefore, PLL for DVR is very important. This paper proposes the new method of PLL in DVR. First, the power circuit of DVR system is analyzed in order to compensate the voltage sags. Based on the analysis, new PLL for improving transient response of DVR is proposed. The proposed method uses band rejection filter(BRF) at q-axis in synchronous flame. In order to calculate compensation voltage in commercial instruments, the PQR theory is used. Proposed PLL method is demonstrated through simulation using Matlab-Simulink and experiment, and by checking load voltage, confirms operation of the DVR

Experimental Investigation of the Electrostatic Discharge(ESD) Damage in Packaged Semiconductor Devices (패키지 반도체소자의 ESD 손상에 대한 실험적 연구)

  • Kim, Sang-Ryull;Kim, Doo-Hyun;Kang, Dong-Kyu
    • Journal of the Korean Society of Safety
    • /
    • v.17 no.4
    • /
    • pp.94-100
    • /
    • 2002
  • As the use of automatic handling equipment for sensitive semiconductor devices is rapidly increased, manufacturers of electronic components and equipments need to be more alert to the problem of electrostatic discharges(ESD). In order to analyze damage characteristics of semiconductor device damaged by ESD, this study adopts a new charged-device model(CDM), field-induced charged model(FCDM) simulator that is suitable for rapid, routine testing of semiconductor devices and provides a fast and inexpensive test that faithfully represents ESD hazards in plants. High voltage applied to the device under test is raised by the field of non-contacting electrodes in the FCDM simulator, which avoids premature device stressing and permits a faster test cycle. Discharge current and time are measured and calculated. The characteristics of electrostatic attenuation of domestic semiconductor devices are investigated to evaluate the ESD phenomena in the semiconductors. Also, the field charging mechanism, the device thresholds and failure modes are investigated and analyzed. The damaged devices obtained in the simulator are analyzed and evaluated by SEM. The results obtained in this paper can be used to prevent semiconductor devices form ESD hazards and be a foundation of research area and industry relevant to ESD phenomena.