• 제목/요약/키워드: Sensing layer

검색결과 490건 처리시간 0.022초

살모넬라균 검출을 위한 임피던스 바이오센서의 항체 고정화 방법 평가 (Evaluation of Antibody Immobilization Methods for Detection of Salmonella using Impedimetric Biosensor)

  • 김기영;문지혜;엄애선;양길모;모창연;강석원;조한근
    • Journal of Biosystems Engineering
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    • 제34권4호
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    • pp.254-259
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    • 2009
  • Conventional methods for pathogen detection and identification are labor-intensive and take several days to complete. Recently developed biosensors have shown potential for the rapid detection of foodborne pathogens. In this study, an impedimetric biosensor was developed for rapid detection of Salmonella typhimurium. To develop the biosensor, an interdigitated microelectrode (IME) was fabricated by using semiconductor fabrication process. Anti-Salmonella antibodies were immobilized based on either avidin-biotin binding or self assembled monolayer (SAM) on the surface of the IME to form an active sensing layer. To evaluate effect of antibody immobilization methods on sensitivity of the sensor, detection limit of the biosensor was analyzed with Salmonella samples innoculated in phosphate buffered saline (PBS) or food extract. The impedimetric biosensor based on SAM immobilization method produced better detection limit. The biosensor could detect 107 CFU/mL of Salmonella in pork meat extract. This method may provide a simple, rapid, and sensitive method to detect foodborne pathogens.

Bi-electrolyte Carbon Dioxide Gas Sensor Based on Paste Sodium-Beta Alumina and Yttria-stabilized Zirconia

  • Han, Hyeuk Jin;Park, Chong Ook
    • 센서학회지
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    • 제23권3호
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    • pp.170-172
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    • 2014
  • $CO_2$ sensor was used only one solid electrolyte in many cases. To improve the sensing characteristics of $CO_2$ sensors, solid electrolyte $CO_2$ sensor has been developed by bi-electrolyte type sensor using Na-Beta-alumina and YSZ. However, in many further studies, bi-electrolyte type sensor was made by pellet pressed by press machine and additional treatment for formation of interface. In the aspect of mass production, using thick film and additional treatment is not suitable. In this study, $CO_2$ sensor was fabricated by bi-electrolyte structure which was made by an NBA paste layer deposited on YSZ pellet and fired at $1650^{\circ}C$ for 2 hour. The formation of stable interface between YSZ and NBA were confirmed by SEM image. When the type IV electrochemical cell arrangement represented by $CO_2,O_2,Pt{\mid}Li_2CO_3-CaCO_3{\parallel}NBA{\parallel}YSZ{\mid}O_2,Pt$ is used to measure the $CO_2$ concentration in air. This sensor EMF should depend only on the concentration of $CO_2$ by logarithmic. Also, sensor shows $P_{CO_2}$ and EMF relationship like nerstian reaction at a temperature of $450^{\circ}C$.

Verification of the Wind-driven Transport in the North Pacific Subtropical Gyre using Gridded Wind-Stress Products Constructed by Scatterometer Data

  • Aoki, Kunihiro;Kutsuwada, Kunio
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2007년도 Proceedings of ISRS 2007
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    • pp.418-421
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    • 2007
  • Using gridded wind-stress products constructed by satellite scatterometers (ERS-1, 2 and QSCAT) data and those by numerical weather prediction(NWP) model(NCEP-reanalysis), we estimate wind-driven transports of the North Pacific subtropical gyre, and compare them in the central portion of the gyre (around 300 N) with geostrophic transports calculated from historical hydrographic data (World Ocean Database 2005). Even if there are some discrepancies between the wind-driven transports by the QSCAT and NCEP products, they are both in good agreement with the geostrophic transports within reasonable errors, except for the regional difference in the eastern part of the zone. The difference in the eastern part is characterized by an anticyclonic deviation of the geostrophic transport resulting from an anti-cyclonic anomalous flow in the surface layer, suggesting that it is related to the Eastern Gyral produced by the thermohaline process associated with the formation of the Eastern Subtropical Mode Water. We also examine the consistency of the Sverdrup transports estimated from these products by comparing them with the transports of the western boundary current, namely the Kuroshio regions, in previous studies. The net southward transport, based on the sum of the Sverdrup transports by QSCAT and NCEP products and the thermohaline transport, agrees well with the net northward transport of the western boundary current, namely the Kuroshio transport. From these results, it is concluded that the Sverdrup balance can hold in the North Pacific subtropical gyre.

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POTENTIAL OF MULTI-BAND SAR DATA FOR CLASSIFYING FOREST COVER TYPE

  • Shin, Jung-Il;Yoon, Jong-Suk;Kang, Sung-Jin;Lee, Kyu-Sung
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2007년도 Proceedings of ISRS 2007
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    • pp.258-261
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    • 2007
  • Although there have been lack of studies using X-band SAR data particularly for forestry application as compared to C-, and L-band SAR data, it has a potential to distinguish tree species because most signals are backscattered on the top of canopy. This study aimed to compare signal characteristics of multi-band SAR data including X-band for classifying tree species. The data used for the study are SIR-C/X-SAR data (X-, C-, L-band) obtained on Oct. 3, 1994 over the forest area near Seoul, S. Korea. Thirty ground sample plots were collected per each tree species. Initial comparison of backscattering coefficients among three SAR bands shows that X-band data showed better separation of tree species than C- and L-band SAR data irrespective of polarization. The weak penetrating in canopy layer might be possible source of information for X-band data to be useful for the classification of forest species and cover type mapping.

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다이아프램 구조를 이용한 탄소나노튜브 가스 센서의 가스감응특성 (Gassensing characteristics of carbon nanotube gas sensor using a diaphragm structure)

  • 김성운;한재천;조우성;주병권;조현섭;김영조
    • 한국산학기술학회:학술대회논문집
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    • 한국산학기술학회 2006년도 춘계학술발표논문집
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    • pp.203-206
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    • 2006
  • The sensor consists of a heater, an insulating layer, a pair of contact electrodes, and CNT-sensing film on a micromachined diaphragm. The heater plays a role in the temperature change to modify sensor operation. Gas sensor responses of CNT-film to $NO_2$ at room temperature are reported. The sensor exhibits a reversible response with a time constant of a few minutes at thermal treatment temperature of $130^{\circ}C$.

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Rapid Detection of Salmonella enteritidis in Pork Samples with Impedimetric Biosensor: Effect of Electrode Spacing on Sensitivity

  • Kim, Gi-Young;Moon, Ji-Hea;Hahm, Bung-Kwon;Morgan, Mark;Bhunia, Arun;Om, Ae-Son
    • Food Science and Biotechnology
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    • 제18권1호
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    • pp.89-94
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    • 2009
  • Frequent outbreaks of foodborne illness have been increasing the awareness of food safety. Conventional methods for pathogen detection and identification are labor-intensive and take days to complete. Some immunological, rapid assays are developed, but these assays still require prolonged enrichment steps. Recently developed biosensors have shown potential for the rapid detection of foodborne pathogens. In this study, an impedimetric biosensor was developed for rapid detection of Salmonella entritidis in food sample. To develop the biosensor, an interdigitated microelectrode (IME) was fabricated by using a semiconductor fabrication process. Anti-Salmonella antibodies were immobilized based on neutravidin-biotin binding on the surface of the IME to form an active sensing layer. To evaluate the effect of electrode gap on sensitivity of the sensor, 3 types of sensors with different electrode gap sizes (2, 5, and $10{\mu}m$) were fabricated and tested. The impedimetric biosensor could detect $10^3\;CFU/mL$ of Salmonella in pork meat extract with an incubation time of 5 min. This method may provide a simple, rapid, and sensitive method to detect foodborne pathogens.

SOG(Silicon On Glass)공정을 이용한 수평형 미소가속도계의 제작에 관한 연구 (A Study on the Fabrication of the Lateral Accelerometer using SOG(Silicon On Glass) Process)

  • 최범규;장태하;이창길;정규동;김종팔
    • 센서학회지
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    • 제13권6호
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    • pp.430-435
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    • 2004
  • The resolution of the accelerometer, fabricated with MEMS technology is mainly affected by mechanical and electrical noise. To reduce mechanical noise, we have to increase mass of the structure part and quality factor related with the degree of vacuum packaging. On the other hand, to increase mass of the structure part, the thickness of the structure must be increased and ICP-RIE is used to fabricate the high aspect ratio structure. At this time, footing effect make the sensitivity of the accelerometer decreasing. This paper presents a hybrid SOG(Silicon On Glass) Process to fabricate a lateral silicon accelerometer with differential capacitance sensing scheme which has been designed and simulated. Using hybrid SOG Process, we could make it a real to increase the structural thickness and to prevent the footing effect by deposition of metal layer at the bottom of the structure. Moreover, we bonded glass wafer to structure wafer anodically, so we could realize the vacuum packaging at wafer level. Through this way, we could have an idea of controlling of quality factor.

Quorum quenching for effective control of biofouling in membrane bioreactor: A comprehensive review of approaches, applications, and challenges

  • Kose-Mutlu, Borte;Ergon-Can, Tulay;Koyuncu, Ismail;Lee, Chung-Hak
    • Environmental Engineering Research
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    • 제24권4호
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    • pp.543-558
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    • 2019
  • In comparison to alternative advanced wastewater treatment technologies, the main problem associated with membrane bioreactor (MBR) technology, which has become prominent in recent years, is biofouling. Within these systems, biofouling is typically the result of a biofilm layer resulting from bacterial gathering. One biological system that can be employed to interrupt the process of bacterial gathering is called 'Quorum Quenching (QQ)'. Existing QQ applications can be classified using three main types: 1) bacterial/whole-cell applications, 2) direct enzyme applications, and 3) natural sourced compounds. The most common and widely recognized applications for membrane fouling control during MBR operation are bacterial and direct enzyme applications. The purpose of this review was to identify and assess biofilm formation mechanism and results, the suggestion of the QQ concept and its potential to control biofilm formation, and the means by which these QQ applications can be applied within the MBR and present QQ MBR studies.

의료용 플라스틱 광섬유 센서 매트 (Sensor Mat using POF for Medical Application)

  • 최규남
    • 전자공학회논문지SC
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    • 제44권4호통권316호
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    • pp.74-78
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    • 2007
  • 의료용으로 사용되는 새로운 개념의 환자감시용 센서 매트와 그 신호처리방식을 제시하였다. 제시된 센서 매트와 플라스틱 광섬유를 교차시켜 배치한 구조를 갖는 내부 센서층을 갖는 구조로 제작하였다. 플라스틱 광섬유의 대구경 특성을 이용하여 불필요한 외부환경 요인에 의하여 발생되는 잡음요소들을 평균화하여 신호성분만을 통과시키는 대역통과필터를 구현하였다. 센서 출력단에 신호처리부를 부가하여 추가로 불필요한 성분들을 제거하여 잡음제거 능력을 향상시켰고 지능형 알고리즘을 사용하여 신뢰성 있는 환자호흡감지방식을 구현하였다. 신호와 주변잡음 모두에게 고감도를 보이는 기존의 기계적인 방식을 사용하는 감지방식과는 달리 본 연구에서 제시된 방식은 신뢰성 있는 호흡 감지가 가능함을 보여주었다.

An analytical solution for static analysis of a simply supported moderately thick sandwich piezoelectric plate

  • Wu, Lanhe;Jiang, Zhiqing;Feng, Wenjie
    • Structural Engineering and Mechanics
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    • 제17권5호
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    • pp.641-654
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    • 2004
  • This paper presents a theoretic model of a smart structure, a transversely isotropic piezoelectric thick square plate constructed with three laminas, piezoelectric-elastic-piezoelectric layer, by adopting the first order shear deformation plate theory and piezoelectric theory. This model assumes that the transverse displacements through thickness are linear, and the in-plane displacements in the mid-plane of the plate are not taken to be account. By using Fourier's series expansion, an exact Navier typed analytical solution for deflection and electric potential of the simply supported smart plate is obtained. The electric boundary conditions are being grounded along four vertical edges. The external voltage and non-external voltage applied on the surfaces of piezoelectric layers are all considered. The convergence of the present approach is carefully studied. Comparison studies are also made for verifying the accuracy and the applicability of the present method. Then some new results of the electric potentials and displacements are provided. Numerical results show that the electrostatic voltage is approximately linear in the thickness direction, while parabolic in the plate in-plane directions, for both the deflection and the electric voltage. These results are very useful for distributed sensing and finite element verification.