• 한국양식학회지
• /
• 제16권3호
• /
• pp.187-189
• /
• 2003

To test the effect of different carbohydrate sources, wheat flour, used as the carbohydrate source in the control diet, was replaced with 30% glucose, 30% maltose, 30% cellulose or 30% $\alpha$-starch. After feeding trial for 8 weeks, no significant differences were found in growth and proximate composition of edible fraction of the snail fed diets containing different carbohydrate sources. All the tested carbohydrates are shown as good dietary carbohydrate source.

• Genomics & Informatics
• /
• 제19권3호
• /
• pp.32.1-32.10
• /
• 2021

Semisulcospira libertina, a species of freshwater snail, is widespread in East Asia. It is important as a food source. Additionally, it is a vector of clonorchiasis, paragonimiasis, metagonimiasis, and other parasites. Although S. libertina has ecological, commercial, and clinical importance, its whole-genome has not been reported yet. Here, we revealed the genome of S. libertina through de novo assembly. We assembled the whole-genome of S. libertina and determined its transcriptome for the first time using Illumina NovaSeq 6000 platform. According to the k-mer analysis, the genome size of S. libertina was estimated to be 3.04 Gb. Using RepeatMasker, a total of 53.68% of repeats were identified in the genome assembly. Genome data of S. libertina reported in this study will be useful for identification and conservation of S. libertina in East Asia.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2002년도 춘계 수산관련학회 공둥학술발표회
• /
• pp.344-345
• /
• 2002

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2002년도 춘계 수산관련학회 공둥학술발표회
• /
• pp.346-347
• /
• 2002

• Preventive Nutrition and Food Science
• /
• 제8권3호
• /
• pp.239-244
• /
• 2003

Recently, we reported (J Korean Soc Food Sci Nutr, 31(3): 516-520, 2002) that Semisulcospira libertina (Marsh Snail) pretreatment has a hepatoprotective effect on $CCl_4$-induced liver damage in rats. The purpose of this study was to investigate the possible mechanisms of hepatoprotection by S. libertina (SL) on liver injury induced by acetaminophen (AA). Male ICR mice were pretreated with dehydrated powder of SL once daily for three consecutive days, given a single toxic dose of AA (450 mg/kg) and liver function determined 24 h later. Liver damage was assessed by quantifying serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and sorbitol dehydrogenase (SDH) activities, and by measuring hepatic lipid peroxidation. To confirm possible mechanism(s), the content of hepatic glutathione (GSH) and gene expression of tumor necrosis factor a (TNF $\alpha$) mRNA by reverse transcription-polymerase chain reaction (RTPCR) were also measured. Pretreatment with SL dramatically lowered AA-elevated ALT, AST and SDH activities. SL pretreatment decreased AA-produced lipid peroxidation by 11% and restored the AA-depleted hepatic GSH by 27%. Furthermore, SL markedly suppressed the expression of TNF $\alpha$ mRNA induced by AA. Our findings revealed that the possible hepatoprotective mechanisms of SL could be attributed, at least in part, to the glutathione-mediated detoxification as well as the regulation of TNF $\alpha$ mRNA expression.

• 한국패류학회지
• /
• 제26권4호
• /
• pp.275-283
• /
• 2010

Many freshwater snail taxa are difficult to identify using morphological traits due to phenotypic plasticity. However, using of molecular DNA marker in combination with morphological traits can provide a reliable means for discriminating among freshwater snail taxa including cryptic species. To discriminate among Korean freshwater snail taxa and resolve their systematic relationships, wesequenced a fragment of mtDNA cytochrome oxidase I (COI) gene from 82 specimens collected from ten different sites distributed along the Korean peninsula. We identified more than seven freshwater snail taxa including cryptic species in Korea. Whereas traditional shell morphology of freshwater snails offers only weak discriminatory power for recognizing 'good' taxa, DNA sequence data provided positive and reliable identification. In addition, a major Semisulcospira clade was clearly separated from the remaining lineages observed including cryptic species. However, a phylogenetic tree inferred from the COI gene data did not fully resolve systematic relationships among pleurocerid taxa in Korea. Establishing more robust shell characteristics for identifying taxa unambiguously and hence improving traditional key shell morphology characters for freshwater snail species is an urgent requirement and will require more rigorous examination of all nominal taxa. While molecular data generated here will be useful for species identification and for describing the systematic relationships among Korean freshwater snails, further analysis will be required.

• 한국수산과학회지
• /
• 제43권3호
• /
• pp.188-194
• /
• 2010

The in vitro antioxidant activities of five melania snails in the genus Semisulcospira (S. coreana, S. forticosta, S. libertina, S. tegulata and S. gottschei) were tested in detail. The total phenolic contents of the snails ranged from $32.3{\pm}1.0$ to $87.9{\pm}6.9$ mg gallic acid equivalent (GAE)/g dry sample. The $EC_{50}$ values for the 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were $2.245{\pm}0.179$ mg/mL for S. coreana and $9.319{\pm}1.276$ mg/mL for S. gottschei and differed significantly (P<0.05) among the tested species. The superoxide dismutase (SOD)-like activity was highest for S. gottschei at 67.2% and lowest for S. forticosta at 4.7%. However, no significant differences among the species were recognized for the peroxynitrite anion scavenging activity. Comparing the correlation coefficients between the total phenolic contents and the DPPH radical and peroxynitrite anion scavenging activities, there was a low level relationship between each activity.

• 한국수산과학회지
• /
• 제36권5호
• /
• pp.454-457
• /
• 2003

A feeding trial was carried out to investigate the utilization of dietary soybean meal and wheat flour as substitutes for fish meal and alginate in different dietary types (powder, pellet and flake) for juvenile snail (Semisulcospira gottschei). After 10 weeks feeding trial, survival was not affected by formulation and type of diets (P>0.05), but weight gain was significantly affected by dietary formulation (P<0.001) and type (P<0.05). In the same dietary formulation, weight gain of the snail fed the flake type of diets was lower than that of the snail fed the powder or pellet types. However, the type of diet did not affect weight gain when snail fed diets containing $37\%$ soybean meal and $18\%$ alginate. The weight gain was lower in the snail fed diets containing $25\%$ fish meal compared with that of the snail for diets containing $33-37\%$ soybean meal at the same dietary type. When soybean meal was used as main protein source in diets, weight gain was not affected by dietary alginate $(0\;and\;18\%)$ and wheat flour $(36\;and\;58\%)$ contents. Crude protein and ash contents of whole body of the snail was not affected either by formulation or by type of diet. Moisture and crude lipid contents of the whole body were significantly affected by dietary formulation, but not by the type. These results indicate that $25\%$ fish meal could be replaced by $37\%$ soybean meal as dietary protein source, and pellet and powder could be desirable dietary type for the optimum growth of the snail.

• 생명과학회지
• /
• 제25권5호
• /
• pp.539-547
• /
• 2015

본 연구는 다슬기(Semisulcospira libertine) 열수 추출물이 D-galactosamine에 의해 급성 간독성이 유도된 흰쥐에 미치는 보호 효과를 조사하였다. 다슬기 열수 추출물은 D-galactosamine에 의해 유발된 간 조직 내 국소적 지방변성과 염증세포 침윤을 크게 완화하여 대조군과 유사하게 보호하는 경향을 보였다. 또한 다슬기 열수 추출물을 처리한 실험군은 간 손상 지표 효소인 AST와 ALT, LDH 및 ALP의 활성이 대조군 수준으로 유지되었으며 간조직 내 지질함량과 과산화지질함량이 감소되는 것으로 나타나 다슬기 열수 추출물이 D-galactosamine으로 인한 혈중 효소 활성과 조직 내 지질함량을 개선하는 것으로 조사되었다. 또한 다슬기 열수 추출물을 처리한 실험군은 염증반응을 촉진시켜 조직 상해 및 괴사를 유도하는 TNF-α의 발현을 억제하고 있어 염증 반응에서 세포 손상을 감소시키는 데 관여하는 것으로 나타났다. 따라서 다슬기 열수 추출물은 D-galactosamine에 의한 조직 괴사를 감소시키고 혈중 효소의 활성과 조직 내 지질함량을 개선하는 효과를 나타내고 있으며 염증 반응 인자의 발현과 항산화효소 활성을 조절하여 간독성에 대해 보호 효과가 높을 것으로 생각된다.

• 한국패류학회지
• /
• 제28권2호
• /
• pp.175-185
• /
• 2012

북한강에 서식하고 있는 참다슬기의 산란생태를 파악하기 위하여 생식소 발달단계에 따른 생식주기, 성비, 군성숙도, 보육낭유생의 월별변화 등 번식생태와 관련된 생물학적 기초 자료를 조사하였다. 각고에 대한 각경, 전중, 육중의 상관계수 ($R^2$) 는 0.7761-0.8719로 비교적 높은 상관관계를 나타내었다. 참다슬기의 암컷 생식소는 청록색, 수컷 생식소는 옅은 황색을 띠고, 암컷은 유생을 기르는 보육낭 (brood pouch) 을 가지고 있었다. 암수성비는 0.17-0.58의 범위로 계절적 불균형이 심하였으며, 여름철 암컷 비율이 높고 겨울철 암컷비율이 낮았다. 생식소의 조직학적 조사 및 비만지수, 생식소지수 조사 결과 봄, 가을에 두 번의 정점을 나타낸 후 급격히 떨어지는 경향을 보여 생식소 발달은 봄, 가을 연 2회 이루어짐을 알 수 있으며, 암컷 보육낭속의 유생수 월별변화도 연중 두 번의 피크를 나타냈었고, 보육낭속의 유생수는 월 평균 286-975범위로, 11월에 연중 가장 적었고 3월과 9월에 각각 975개, 863개로 연중 가장 많은 유생을 보유하였다. 50%가 성숙하는 군성숙체장은 각고 13.95 mm였으며, 각고 15mm 이상은 모든 개체가 성숙하고 보육낭을 형성하였다.