• 한국발생생물학회지:발생과생식
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• 제14권1호
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• pp.35-41
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• 2010

Mammalian reproduction is regulated by a feedback circuit of the key reproductive hormones such as GnRH, gonadotropin and sex steroids on the hypothalamic-pituitary-gonadal axis. In particular, the onset of female puberty is triggered by gain of a pulsatile pattern and increment of GnRH secretion from hypothalamus. Previous studies including our own clearly demonstrated that genistein (GS), a phytoestrogenic isoflavone, altered the timing of puberty onset in female rats. However, the brain-specific actions of GS in female rats has not been explored yet. The present study was performed to examine the changes in the activities of GnRH neurons and their neural circuits by GS in female rats. Concerning the drug delivery route, intracerebroventricular (ICV) injection technique was employed to eliminate the unwanted actions on the extrabrain tissues which can be occurred if the testing drug is systemically administered. Adult female rats (PND 100, 210-230 g BW) were anaesthetized, treated with single dose of GS ($3.4{\mu}g$/animal), and sacrificed at 3 hrs post-injection. To determine the transcriptional changes of reproductive hormone-related genes in hypothalamus, total RNAs were extracted and applied to the semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). ICV infusion of GS significantly raised the transcriptional activities of enhanced at puberty1 (EAP-1, p<0.05), glutamic acid decarboxylase (GAD67, p<0.01) which are known to modulate GnRH secretion in the hypothalamus. However, GS infusion could not change the mRNA level of nitric oxide synthase 2 (NOS-2). GS administration significantly increased the mRNA levels of KiSS-1 (p<0.001), GPR54 (p<0.001), and GnRH (p<0.01) in the hypothalami, but decreased the mRNA levels of LH-$\beta$ (p<0.01) and FSH-$\beta$ (p<0.05) in the pituitaries. Taken together, the present study indicated that the acute exposure to GS could directly activate the hypothalamic GnRH modulating system, suggesting the GS's disrupting effects such as the early onset of puberty in immature female rats might be derived from premature activation of key reproduction related genes in hypothalamus-pituitary neuroendocrine circuit.

• Environmental Engineering Research
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• 제16권3호
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• pp.131-136
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• 2011

The degradation of 30 pharmaceuticals and personal care products (PPCPs) subjected to $O_3$, $O_3$/UV, and $O_3/H_2O_2$ treatments were investigated using semi-batch tests and evaluated by their pseudo-first-order rate constants. The additional application of UV or $H_2O_2$ during $O_3$ treatment significantly improved the degradation rate of most of the PPCPs. At the same $O_3$ feed rate, $O_3$/UV treatment exhibited much higher PPCP degradation efficiency than that of $O_3$ treatment. This was probably due to degradation of the PPCPs by $O_3$, direct UV photodegradation, and OH radicals that formed from the photodegradation of $O_3$ during $O_3$/UV treatment. PPCP degradation by $O_3$ was also promoted by adding $H_2O_2$ during the $O_3$ treatment. However, when the initial $H_2O_2$ concentration was high during $O_3$ treatment, OH radicals were likely to be scavenged by excess $H_2O_2$, leading to low PPCP degradation. Therefore, it is important to determine the appropriate $H_2O_2$ dosage during $O_3$ treatment to improve PPCP degradation when adding $H_2O_2$ during $O_3$ treatment.

• 한국콘텐츠학회논문지
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• 제17권4호
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• pp.25-35
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• 2017

본 연구에서는 한국의 유아 교사들이 창의성 교육에 관한 개념을 어떻게 인식하고 있는지 그 과정에 관하여 탐색하는 것을 목적으로 질적 연구를 수행하였다. 면접대상자는 서울 소재 유치원에 재직하고 있는 현직교사 4명이며, 반구조화된 심층면접을 통하여 면접 자료를 수집하였다. 수집된 자료는 질적 연구방법 중 木下(기노시다)의 수정근거이론(Modified Grounded Theory Approach)을 채택하여 분석하였다. 자료 분석 결과, 유아교육 분야의 전문가 집단이라 할 수 있는 유아 교사들은 독특한 사고, 풍부한 표현력 등이 창의성교육의 핵심개념이라고 파악하고 있었다. 이와 같은 개념은 주로 아이들과의 직접적인 만남이 이루어지는 학습, 누리과정 교수 등을 통해서 창의성 개념을 형성하는 것으로 나타났다. 이보다 앞서 수행된 초 중등 교사들을 대상으로 창의성 개념을 탐색한 선행연구와 비교해 볼 때, 유아 교사들은 창의성 교육에 관하여 상당히 일치된 핵심개념을 서로 공유하고 있다는 것이 본 연구를 통해 밝혀졌다. 본 연구결과는 유아교육 분야에서는 비교적 창의성 교육에 관한 개념이 일관성 있게 받아들여지고 있으며, 교육현장에서도 유아들에게 개념 및 교수방법 등이 양호하게 잘 전달되고 있음을 의미한다.

• Architectural research
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• 제16권2호
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• pp.37-44
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• 2014

Utilizing spaces for awareness could be superficially noble. However, the typology of spaces played an important role in addressing the awareness of translating the messages through tangible forms, which in this context are sculptures. Assuring the efficacy of the typology of the spaces that is compatible with the content is important to ensure its timelessness to the dynamic demographical change of viewers. Therefore, the study proposes an investigation of the efficacy of typology of public urban space; garden that accommodates memorial sculptures. The study intends to analyze the features and flaws of ASEAN Sculpture Garden, as a mediatory role of ASEAN in instilling awareness of unity between ASEAN members to the public. The objective of the study is to analyze the efficacy of the garden to accommodate the rich memorial sculptures. The body of the research is formed by theoretical and case study research. It is projected by three methods; archival research, semi structured interviews and site documentation; direct observations and site visit. The qualitative data then will be analysed using Actor Network theory, keyword coding and site findings. Findings showed that as the main objective of the garden is to exhibit the sculptures, it could be summed up that it has failed to meet the apparent intention based on the site analysis conducted by the researcher. The typology of the space should be able to communicate the project's aims continuously rather than serving the function temporarily. The variables, which are space and time factors are debatable, as the neighboring tourist spot, National Monument residing the wide square managed to attract public attention due to its great efficacy of typological space regardless the time factor. As a conclusion, the typology of space is a huge factor to ensure the park's success.

• The Korean Journal of Physiology and Pharmacology
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• 제22권2호
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• pp.113-125
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• 2018

Exosomes are membranous vesicles of 30-150 nm in diameter that are derived from the exocytosis of the intraluminal vesicles of many cell types including immune cells, stem cells, cardiovascular cells and tumor cells. Exosomes participate in intercellular communication by delivering their contents to recipient cells, with or without direct contact between cells, and thereby influence physiological and pathological processes. They are present in various body fluids and contain proteins, nucleic acids, lipids, and microRNAs that can be transported to surrounding cells. Theragnosis is a concept in next-generation medicine that simultaneously combines accurate diagnostics with therapeutic effects. Molecular components in exosomes have been found to be related to certain diseases and treatment responses, indicating that they may have applications in diagnosis via molecular imaging and biomarker detection. In addition, recent studies have reported that exosomes have immunotherapeutic applications or can act as a drug delivery system for targeted therapies with drugs and biomolecules. In this review, we describe the formation, structure, and physiological roles of exosomes. We also discuss their roles in the pathogenesis and progression of diseases including neurodegenerative diseases, cardiovascular diseases, and cancer. The potential applications of exosomes for theragnostic purposes in various diseases are also discussed. This review summarizes the current knowledge about the physiological and pathological roles of exosomes as well as their diagnostic and therapeutic uses, including emerging exosome-based therapies that could not be applied until now.

• 대한약침학회지
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• 제19권1호
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• pp.28-36
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• 2016

Objectives: The purpose of this study is to achieve a vision for autistic children and their parents aimed at generating interest in ideas such as "Sanitas Per Aquam" (SPA), massage and music therapy, which has begun to have widespread use and to attract attention. Methods: This cross-sectional, descriptive study was carried out with autistic children and their parents from February to April 2015 in $Mu{\breve{g}}la$, Turkey. The study was began by interviewing experts in the field and by developing a suitable assessment questionnaire. In order to direct the flow of conversation between the researchers and the autisitc children and their parents, the researchers conducted semi-structured face to face interviews in a form that had been determined by using reports in the literature and the opinions of experts in the field. Results: Forty two boys (84%) and eight girls (16%) with autism participated in our study. Children in the 0 - 7 age group spent long time in the bathroom (P = 0.001). Boys liked to be hugged more than girls (P = 0.01). Children ages 0 - 7 years liked bright lighting while those 15 years of age and older liked gloomy lighting (P = 0.009). Except for these statistically significant sex- and age-related differences, no other statistically significant differences were noted in the parameters of this study. Although the result was not statistically significant, more children with mild autism disorder obeyed commands like inhale or exhale (P = 0.051). Conclusion: Treatment for autism spectrum disorders is not yet fully possible, so many studies are being done to alleviate some symptoms and to improve the quality of life for individuals with autism and their families. As a result of our study, whether touching the areas the children want touched and listening to their favorite music are required to stimulate the brain remain as questions in our minds.

• Journal of Electrical Engineering and information Science
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• 제3권2호
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• pp.239-244
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• 1998

Piezoelectric pressure sensors and pyroelectric infrared detectors based on ZnO thin film have been integrated with GaAs metal-semiconductor field effect transistor (MESFET) amplifiers. Surface micromachining techniques have been applied in a GaAs MESFET process to form both microsensors and electronic circuits. The on-chip integration of microsensors such as pressure sensors and infrared detectors with GaAs integrated circuits is attractive because of the higher operating temperature up to 200 oC for GaAs devices compared to 125 oC for silicon devices and radiation hardness for infrared imaging applications. The microsensors incorporate a 1${\mu}$m-thick sputtered ZnO capacitor supported by a 2${\mu}$m-thick aluminum membrane formed on a semi-insulating GaAs substrate. The piezoelectric pressure sensor of an area 80${\times}$80 ${\mu}$m2 designed for use as a miniature microphone exhibits 2.99${\mu}$V/${\mu}$ bar sensitivity at 400Hz. The voltage responsivity and the detectivity of a single infrared detector of an area 80${\times}$80 $\mu\textrm{m}$2 is 700 V/W and 6${\times}$108cm$.$ Hz/W at 10Hz respectively, and the time constant of the sensor with the amplifying circuit is 53 ms. Circuits using 4${\mu}$m-gate GaAs MESFETs are fabricated in planar, direct ion-implanted process. The measured transconductance of a 4${\mu}$m-gate GaAs MESFET is 25.6 mS/mm and 12.4 mS/mm at 27 oC and 200oC, respectively. A differential amplifier whose voltage gain in 33.7 dB using 4${\mu}$m gate GaAs MESFETs is fabricated for high selectivity to the physical variable being sensed.

• 대한화학회지
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• 제43권6호
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• pp.644-650
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• 1999

본연구에서 유기 침전제를 사용하여 수용액중의 코발트를 on-line으로 연속 침전시켜 선농축하는 방법을 연구하였다. 침전제로 1-nitroso-2-naphthol을 사용하여 라인내에서 직접 침전시킨 뒤 MlBK(Methyl lsobutyl Ketone)로 용해시켜 불꽃 원자흡수 분광기로 보내어 분석하였다. 최적조건을 구하고 농축배율을 연구한 결과 대개 0.5 ppm의 코발트 시료 1.0 mL를 사용하였을 때 13배의 농축율을 얻었으며 시료 처리 속도는 시간당 I0개 정도 이었다. 부피를 10.0 mL로 증가시키면 시료의 처리속도는 늦어지지만 농축비를 68배로 높일 수 있었다. 본 연구의 정확성을 알기 위해 합성된 준 표준 생체시료를 분석한 결과 4%의 상대 표준편차를 가지고 예상치에 근접하는 좋은 결과를 얻을 수 있었다.

• Animal cells and systems
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• 제11권2호
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• pp.141-146
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• 2007

Cantharidin is produced by blister beetles (Coleoptera: Meloidae) and smaller oedemerid beetles (Coleopetra: Oedemeridae) and is found in hemolymph and various tissues. The function of cantharidin in the courtship behaviour of meloids had never been fully established. Our studies show a correlation between density of cuticular pores and cantharidin titre of the scape and pedicel segments of male specimens of the East African species of Epicauta nyassensis (Haag-Rutenberg, 1880) (Coleoptera: Meloidae). Light microscopy of semi-thin cross sections of the male scape and pedicel indicates that there are many canal shaped structures that stretch from the antennal hemolymph to the antennomere surface. These structures may be tubules, which transport cantharidin circulating in the hemolymph to the surface, where the compound can be released via cuticular pore openings. Analyses of the head capsule and antennal segments of E. nyassensis females which had been copulated with males revealed low titre of cantharidin in the first two antennal segments. The density of the scape and pedicel pores of females was to some extent higher than the density of these pores on flagellum; however it was considerably lower than that of the males. Interestingly, no tubular cell or other transport structures were found in the cross sectioning of the female antennomeres or on the integument surface. During mating, male antennomeres, as well as cantharidin containing pores which are located on the $1^{st}\;and\;2^{nd}$ antennomeres, come into direct contact with the female antennae and may release cantharidin to their surface. Female E. nyassensis may be able to discriminate the opposite sex with abundant reserves of cantharidin prior to mating. This is another evidence that cantharidin function in close range sexual selection.

• 대한영양사협회학술지
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• 제17권2호
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• pp.161-175
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• 2011

This study examined students' menu preferences as well as the job satisfaction and specific considerations concerning foodservice operation of dieticians and nutrition teachers at special schools for the disabled. Semi-structured interview questionnaires were sent to all of the 21 special schools with direct school food service in Gyeonggi-do, and 16 dieticians and nutrition teachers agreed to participate in this study. Among the various menu groups, the subjects perceived the meat group as the most preferred and the vegetable group the least preferred. They were generally satisfactory with their job and duty, and had a strong sense of belonging to the school. However, most of them answered that they needed more time for nutritional education and counseling. While most had pride and pleasure in their job, they expressed a desire to change the system to better fit with their roles as professional nutritionists. The content analysis revealed that the subjects mainly focused on safety issues in planning the food menu and were thus limited in selecting menu items. They also felt difficulty in menu planning due to various tastes and preferences, since special schools tend to consist of a wider grade range. However, most subjects reported little trouble in food distribution and food leftovers owing to practical support from teachers and parents. The necessity for education concerning table manners and obesity prevention was generally a shared opinion, and education programs for parents were also perceived as necessary to better understand the special considerations for developing proper eating habits in their child. The study findings provide useful basic data to improve the foodservice system at special schools.