• 한국발생생물학회지:발생과생식
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• 제21권1호
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• pp.47-54
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• 2017

Unlike mouse results, cloning efficiency of nuclear transfer from porcine induced pluripotent stem cells (piPSCs) is very low. The present study was performed to investigate the effect of cell cycle inhibitors on the cell cycle synchronization of piPSCs. piPSCs were generated using combination of six human transcriptional factors under stem cell culture condition. To examine the efficiency of cell cycle synchronization, piPSCs were cultured on a matrigel coated plate with stem cell media and they were treated with staurosporine (STA, 20 nM), daidzein (DAI, $100{\mu}M$), roscovitine (ROSC, $10{\mu}M$), or olomoucine (OLO, $200{\mu}M$) for 12 h. Flow Cytometry (FACs) data showed that piPSCs in control were in G1 ($37.5{\pm}0.2%$), S ($34.0{\pm}0.6%$) and G2/M ($28.5{\pm}0.4%$). The proportion of cells at G1 in DAI group was significantly higher than that in control, while STA, ROSC and OLO treatments could not block the cell cycle of piPSCs. Both of viability and apoptosis were affected by STA and ROSC treatment, but there were no significantly differences between control and DAI groups. Real-Time qPCR and FACs results revealed that DAI treatment did not affect the expression of pluripotent gene, Oct4. In case of OLO, it did not affect both of viability and apoptosis, but Oct4 expression was significantly decreased. Our results suggest that DAI could be used for synchronizing piPSCs at G1 stage and has any deleterious effect on survival and pluripotency sustaining of piPSCs.

• 대한한방내과학회지
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• 제19권1호
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• pp.221-246
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• 1998

The aim of this experimental study was to evaluate the anti-tumor effects of Chungsimbohyeltang through investigation about viability of tumor cell by MTT assay, survival period of mice transplanted with L-1210 cells, growth inhibition on the tumor cell, body weight variation in mice transplanted with sarcoma-180 cells and its immunomodulatory effects through the investigation on delayed type hyper-sensitivity, the hemagglutinin and hemolysin titers for humoral immune response, the appearance of rosette forming cells for cell-mediated immune response, the natural killer cell activity, the transformation of lymphocyte, the productivity of Interleukin-2 and phagocytic index K was performed in immune-depressed ICR mice induced by methotrexate treatments. The results were as follows ; 1. $IC_{50}$ of Chungsimbohyeltang treated group was 5.85mg/ml in SNU-C4 cell, 1.38mg/ml in SNU-396 cell, 0.21mg/ml in SNU-1 cell, so it had low anti-tumor activity. 2. The both groups of Chungsimbohyeltang extract 10mg/kg and Chungsimbohyeltang extract 20mg/kg had no toxicity and the group of Chungsimbohyeltang 20mg/kg which was shown 120% in ILS had the effect of life prolongation in mice transplanted with L-1210 cells. 3. In the growth inhibition on the tumor cells, only the group of Chungsimbohyeltang extract 20mg/kg was noted and in the weight variation in mice transplanted with sarcoma-180 cells, both groups of Chungsimbohyeltang extract had a significant effect. 4. In the delayed type hypersensitivity and appearance of rosette forming cells, both groups of Chungsimbohyeltang extract didn't have any significant effect. 5. The hemagglutinin titers was slightly increased with no significance, and the hemolysin titers was significantly increased in the only group of Chungsimbohyeltang extract 20mg/kg. 6. The natural killer cell activity of the Chungsimbohyeltang extract groups was significantly increased in the ratio of 100:1 of effector and target cells, but it was not significantly increased in the ratio of 50:1, 10:1. 7. The transformation of lymphocyte and the productivity of Interleukin-2 were increased significantly and in dose-dependent manner in both group of Chungsimbohyeltang extract. 8. The phagocytic effect of macropage was significantly increased in both groups of Chungsimbohyeltang extract. Considering the results above, we could conclude that Chungsimbohyeltang have an indirect anti-tumor effect through the modulation of immunme response, although it had not toxicity on the tumor cell it self.

• Asian Pacific Journal of Cancer Prevention
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• 제15권5호
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• pp.2165-2168
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• 2014

Background: Today, survival rate of patients with chronic renal failure/hemodialysis has increased so that chronic illnesses are more likely to occur. Cancer is the main cause of morbidity and mortality in such patients. Aim: In this study, physician attitudes were examined about cancer screening in patients with renal failure. Materials and Methods: This study was done by face to face questionnaire in the $27^{th}$ National Nephrology Congress to determine if the physicians dealing with chronic renal failure, hemodialysis or renal transplanted patients, recommend cancer screening or not and the methods of screening for cervix, prostate, breast and colon cancer. Results: One hundred and fifty six physicians were included in the survey. A total of 105 (67%) participants were male and the age of responders was $48{\pm}9$ years. About 29% were specialists in nephrology, 28% internal medicine, and 5% were other areas of expertise. Some 48% of participants were hemodialysis certified general practitioners. Patients were grouped as compensated chronic renal failure, hemodialysis or renal transplanted. Of the 156 responders, 128 (82%) physicians recommended breast cancer screening and the most recommended subgroup was hemodialysis patients (15%). The most preferred methods of screening were combinations of mammography, self breast examination and physicianbreast examination. 112 (72%) physicians recommended cervix cancer screening, and the most preferred method of screening was pap-smear. Colon cancer screening was recommended by 102 (65%) physicians and prostate screening by 109 (70%) physicians. The most preferred methods of screening were fecal occult blood test and PSA plus rectal digital test, respectively. Conclusions: It is not obvious whether cancer screening in renal failure patients is different from the rest of society. There is a variety of screening methods. An answer can be found to these questions as a result of studies by a common follow-up protocol and cooperation of nephrologists and oncologists.

• 디자인학연구
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• 제11권1호
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• pp.173-184
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• 1998

본 연구는 미래 도시경관 ·건축환경에서 요구되는 미학적 질에 관한 연구로 다수가 선호하고 즐거워할 수 있는 과학적이고 객관적인 미적 가치, 시각적 특성들을 제안하는 것이 목표이다. 연구의 이론적 접근방법은 경험 과학적 미학으로 이를 통한 인간지각.인지.행동의 본성을 이해하고자 하였으며 미적 환경이 갖는 시각적 질의 특성을 범주화하고 그 효과를 설명, 예측하고자 하였다. 그리고 환경지각.인지이론과 새롭게 제시된 환경미학모델들을 검토하였으며 환경디자인에 적용될 수 있는 미학적 특성들을 제안하였다. 첫째, 시각적 질에 대한 미적 경험은 심리 생물학적 특성과 인간활동의 결합과정으로 환경의 형식적.상징적 그리고 내적인 표상체계의 상호작용에 의해서 이루어짐을 알 수 있다. 또한 시각적 질은 사회 문화적 가치, 개인적.집단적 가치 등으로 인해 다양성과 차이가 있으므로 절대화 할 수 없는 복잡한 영역으로 간주되었다. 둘째, 시각적 질의 효과, 미적 반응은 궁극적으로 즐거움, 선호, 인간생존을 위한 적응과 자율성 등에 관계된다. 셋째, 미학은 더욱더 통합적인 학제간 연구를 통해 발전될 수 있으며 이러한 미학적 경험자료는 디자인, 계획, 평가를 위한 기초적인 정보가 될 수 있을 것으로 판단된다.

• Fisheries and Aquatic Sciences
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• 제21권4호
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• pp.10.1-10.8
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• 2018

Substitution effect of white radish' by-product (WRB) and tunic of sea squirt (TSS) for Undaria in feed on growth and carcass composition of abalone was determined. Eight-hundred forty juveniles were distributed into 12 containers. Three formulated diets were prepared in triplicate. A 15% Undaria was included in the control diet. The 15% Undaria was substituted with a same amount of WRB and TSS, referred to as the DRG and TSS diets, respectively. Finally, dry Undaria was also prepared to compare effect of the formulated diets on performance of abalone. The experimental diets were fed to abalone once a day at a satiation level with a little leftover. The feeding trial lasted for 16 weeks. Crude protein, crude lipid, and ash content of the experimental diets changed over all period of time. Higher crude protein and lipid and ash contents retained in all formulated diets compared to the Undaria were observed at 24 and 48 h after seawater immersion. Survival of abalone was not affected by the experimental diets. Weight gain and specific growth rate (SGR) of abalone fed the TSS diet was greater than all other diets. Weight gain and SGR of abalone fed the control diet was not different from those of abalone fed the WRB diet. Proximates of the soft body of abalone was affected by the diets. In conclusion, WRB and TSS are the promising feed ingredients to replace Undaria in abalone feed; especially, TSS is superior to either Undaria or WRB. The formulated diets produced improved growth performance of abalone over the single Undaria.

• 디지털융복합연구
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• 제11권10호
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• pp.537-547
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• 2013

최근 사회의 급격한 변화는 사회내 모든 부분에 반영되어 성과와 효율에 대한 무한 경쟁의 모습으로 나타나고 있다. 교육 분야도 이 같은 상황은 예외가 아니어서 수요자의 요구를 충족시키기 위한 형태로 대학간 전공간 경쟁이 심화되고 있다. 주문식 교육제도의 도입은 바로 이러한 특성을 반영한 대학의 생존전략 중에 하나이다. 치위생학의 주문식 교육과정에 대한 연구결과 치과병의원의 관리자들은 치과진료보조분야의 직무를 치과위생사의 주요업무라고 생각하는 것으로 나타났다. 반면 치과위생사들은 구강보건교육을 주요업무로 생각하고 있었으나 실제에 있어서는 본인의 의사와 달리 치과병의원 관리자의 의사를 따르는 것으로 나타나 1차적으로 산업체의 요구에 따른 산업체 현장실습강화가 필요한 것으로 분석되었다. 그리고 이와 함께 치과위생사의 주요 역할인 예방치과처치, 구강보건교육을 강화함으로써 병의원 내에서 치과위생사의 지위가 안정적으로 정착되게 하여 직장생활을 통해 자아실현의 기회를 제공할 필요도 있음을 알 수 있었다.

• 대한지역사회영양학회지
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• 제19권2호
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• pp.163-175
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• 2014

Objectives: We aimed to evaluate dietary intake among female breast cancer survivors in a cross-sectional study. Methods: A total of 127 women who had breast cancer surgery at least 6 months before baseline were included. Dietary intake of female breast cancer survivors was assessed through self-reported 3 day-dietary records. To compare dietary intake between breast cancer survivors and general female population without cancer, we selected the 1:4 age matched women from the 2011 Korean National Health and Nutrition Examination Survey (KNHANES). In the KNHANES, participants were asked about their dietary intake using the 24-hour dietary recalls. We also examined whether dietary intake varied by age group, cancer stage, or time since surgery among breast cancer survivors. We used the generalized linear model to compare their dietary intakes. Results: Intakes of total energy, beta-carotene, folate, vitamin C, plant iron and fruits were lower among breast cancer survivors with longer time since surgery compared to those with shorter time (p<0.05). Breast cancer patients with higher stage at diagnosis tended to consume less legumes (p=0.01) than those with lower stage. When we compared dietary intake between breast cancer survivors and the general female population without cancer, breast cancer survivors were more likely to consume most of macro- and micro-nutrients in larger quantity (p<0.05) and adhere to healthier diet characterized by higher intakes of legumes, seed and nuts, vegetables and fishes and shells than the general female population who never had been diagnosed with cancer (p<0.05). Conclusions: Our study results suggested that the intakes of nutrients and foods varied by time since surgery and cancer stage among breast cancer survivors and dietary intakes among breast cancer survivors differed from that in the general population. Further prospective studies are warranted to explore the association between dietary intakes of specific food items and survival among Korean breast cancer survivors.

• Gut and Liver
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• 제12권6호
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• pp.722-727
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• 2018

Background/Aims: Although endoscopic bilateral stent-instent placement is challenging, many recent studies have reported promising outcomes regarding technical success and endoscopic re-intervention. This study aimed to evaluate the technical accessibility of stent-in-stent placement using large cell-type stents in patients with inoperable malignant hilar biliary obstruction. Methods: Forty-three patients with inoperable malignant hilar biliary obstruction from four academic centers were prospectively enrolled from March 2013 to June 2015. Results: Bilateral stent-in-stent placement using two large cell-type stents was successfully performed in 88.4% of the patients (38/43). In four of the five cases with technical failure, the delivery sheath of the second stent became caught in the hook-cross-type vertex of the large cell of the first stent, and subsequent attempts to pass a guidewire and stent assembly through the mesh failed. Functional success was achieved in all cases of technical success. Stent occlusion occurred in 63.2% of the patients (24/38), with a median patient survival of 300 days. The median stent patency was 198 days. The stent patency rate was 82.9%, 63.1%, and 32.1% at 3, 6, and 12 months postoperatively, respectively. Endoscopic re-intervention was performed in 14 patients, whereas 10 underwent percutaneous drainage. Conclusions: Large cell-type stents for endoscopic bilateral stent-in-stent placement had acceptable functional success and stent patency when technically successful. However, the technical difficulty associated with the entanglement of the second stent delivery sheath in the hook-cross-type vertex of the first stent may preclude large cell-type stents from being considered as a dedicated standard tool for stent-in-stent placement.

• 한국시뮬레이션학회논문지
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• 제30권3호
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• pp.11-17
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• 2021

30mm 대공포는 비호, 비호복합, 차륜형대공포로 다양한 형태의 무기체계로 개발되어 방공 기능의 주요 대공무기로 그 역할을 수행하고 있다. 대공무기는 비행체의 공격으로부터 영공을 방어하는 임무를 수행한다. 특히 공격작전 시 대공무기는 기계화부대와 혼합 편성된다. 그리고 대공무기는 전쟁터의 최전방에서 이동하기 때문에 적에게 공격 받기 쉽다. 적군은 자기의 생존성 보장율을 높이기 위하여 최대한 엄폐나 은폐하면서 아군을 공격할 것으로 예상된다. 따라서 본 연구는 아군의 30mm 대공탄이 엄폐하고 있는 적군을 제압할 수 있는지를 분석하였다. 이 연구는 M&S 기법을 사용하여 30mm 대공탄의 성능을 분석하였다. 본 연구는 실험을 위하여, 실사격과 M&S에 의한 모의 방법을 이용하였다. 본 연구는 실사격 실험을 위하여 강판과 합판을 사용하였다. 또한 M&S를 통한 모의실험 과정에서 본 연구가 30mm 대공무기의 탄도와 관통·파편능력을 분석하기 위하여 PRODAS모델, AUTODYN모델, Split-x모델을 활용하였다. 실험결과에 의하면 30mm 대공탄으로 적의 장갑차량을 파괴할 수 있는 것이 증명되었다. 30mm 대공무기는 일반건물이나 숲속에 은폐한 적을 신속하게 제압하는데 성공하였다. 이로써 사전에 아군의 피해를 최소화할 수 있다.

• 사물인터넷융복합논문지
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• 제8권6호
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• pp.115-122
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• 2022

최근 병원 밖 심정지 환자의 생존율 향상을 위한 일반인 대상 심폐소생술 교육의 중요성이 강조되고 있다. 일반인 대상 효과적인 심폐소생술 교육을 위해 보다 정확하고 생동감 있는 교육전략이 필요하다. 이에 본 연구에서는 확장현실 기반의 심폐소생술 교육시스템을 개발하고 일반인을 대상으로 교육한 사용성 평가 결과를 제시한다. 3개의 응용프로그램으로 구성된 확장현실 기반 심폐소생술 교육시스템에서는 첫 번째 마네킹에 정합된 3D 심장 해부도를 스마트글라스에 전송하여 가슴압박 지점을 안내한다. 두 번째 응용프로그램은 스마트글라스를 통해 심폐소생술 과정에 대한 시·청각적 정보를 제공함과 동시에 스마트워치의 진동 알림을 전송하여 심폐소생술의 정확한 압박 속도를 안내한다. 세번째 Add on kit는 마네킹에 설치된 센서를 통하여 흉부 압박의 깊이와 속도에 대한 즉각적인 피드백 정보를 스마트폰으로 전송한다. 본 연구에 참여한 93명의 대상자는 확장현실 기반 심폐소생술 교육시스템이 현장감과 효과성 측면에서 긍정적이라 평가하였다. 확장현실 기술을 이용한 정합기술은 현장감과 몰입도를 높이고 자기 주도적 훈련을 쉽게 함으로서 심폐소생술 교육 운영 효율성 향상에 이바지할 수 있다.