• Title/Summary/Keyword: Self-Identification

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Isolation and identification of Flavobcterium succinicans from anadromous ayu Plecoglossus altivelis (소하은어의 병소로부터 Flavobcterium succinicans의 분리 및 동정)

  • Lee, Chang-Hoon;Kim, Pil-Youn;Lim, Bong-Soo;Oh, Duck-Chul;Kang, Bong-Jo
    • Journal of fish pathology
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    • v.22 no.3
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    • pp.401-406
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    • 2009
  • On May in 2008, mortality of anadromous Ayu Plecoglossus altivelis was observed on the Gangjeong river in Jeju. Major symptoms of the infected fish were mouth rot and skin ulcer. The causative agent was suspected as gliding bacteria. After culture on Shu-Shott and R2A media, we isolated bacterium belonging to the Flavobacterium from ayu with symptoms. As a result, the bacterium was identified as Flavobacterium succinicans JMFL55 by 16S rDNA sequence alignment with F. succinicans DSM 4002(98.27% similarity, GenBank accession NO. AM230492).

Endophytic Yeasts Colonize Roots of Ulmus parvifolia Jacq. and Quercus salicina Blume

  • Kim, Jong-Shik;Kim, Dae-Shin
    • Korean Journal of Environmental Agriculture
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    • v.36 no.2
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    • pp.135-139
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    • 2017
  • BACKGROUND: Identification and characterization of endophytic yeasts inhabiting the roots of Ulmus parvifolia Jacq. and Quercus salicina Blume require biotechnological and culture-based techniques. METHODS AND RESULTS: Homogenized U. parvifolia and Q. salicina root samples were spread onto four types of agar medium containing ancgtibiotics, L-sorbose, and Triton X-100. In total, 25 yeast strains were isolated and subjected to phylogenetic analysis based on their internal transcribed spacer region sequences. The results revealed that the yeast genera Cyberlindnera (12 isolates) and Cryptococcus (1 isolate) were associated with roots of U. parvifolia; and the genera Rhodotorula (8 isolates), Trichosporon (3 isolates), and Kluyveromyces (1 isolate) were associated with roots of Q. salicina. Additionally, a Kluyveromyces isolate produced a detectable level of bioethanol. The yeast strains reported herein may be used in industrial production of biosurfactants and bioethanol. CONCLUSION: Our findings revealed that the endophytic yeast genera Cyberlindnera and Cryptococcus predominated in roots of U. parvifolia; and the genera Rhodotorula (8 isolates), Trichosporon (3 isolates), and Kluyveromyces (1 isolate) predominated in roots of Q. salicina. Additionally, Kluyveromyces isolates produced a detectable level of bioethanol.

Alcohol Consumption Rates and the Perception of Drinking Cultures Among College Students in the Wonju Area (원주지역 대학생들의 음주실태 및 음주문화에 대한 인식)

  • Oh, Hae-Sook
    • Journal of the Korean Society of Food Culture
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    • v.26 no.2
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    • pp.101-112
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    • 2011
  • The objective of this study was to examine alcohol consumption rates and the perception of drinking cultures among college students in the Wonju area. An examination of factors such as frequency of drinking, average quantity consumed, and frequency of heavy drinking suggested that the drinking rates were relatively high. Over 70% of subjects drank at least once a week, 66.2% typically drank more than 5 servings at a time, and 19.2% of males and 13.0% of females were heavy drinkers. It was revealed from an AUDIT (Alcohol Use Disorder Identification Test) assessment that 71.3% of the subjects tested had various levels of alcohol-related problems. These problems were more severe in subjects that were male, selfboarding, or overweight. Alcohol related knowledge was not high because the subjects didn't know or incorrectly recognized some contents such as blood alcohol concentration, the energy content of alcohol, and the empty caloric characteristics of alcohol. Generally male, self-boarding, and overweight persons were not critical of the undesirable characteristics associated with drinking culture. Two opinions that were generally considered to be permissible were: 'Men should be able to drink' and 'Drinking is essential for a smooth human relationship'.

A Study on the Kitsch Characteristics of Hair Style and Head Dress in the 1990s (1990년대(年代) Hair Style과 Head Dress의 키치특성(特性)에 관(關)한 연구(硏究))

  • Koo, In-Sook
    • Journal of Fashion Business
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    • v.5 no.2
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    • pp.73-86
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    • 2001
  • This study aims at reviewing the Kitsch aesthetics, grasping the relation between the Kitsch and consumption culture, and pursuing the intrincis meanings and expressive characteristics of Kitsch in the hair style and head dress in the 1990s. Kitsch generally implies a vulgarity, and a bad taste, and can be considered to be a kind of vicarious experience, the aesthetic deception and self-deception. In spite of these negative inplications of Kitsch, the aesthetic categories of Kitsch have been spread to public and been accepted by the public, with the help of the powerful promotion of the modern day mass media. 1. The intrincis meanings of Kitsch convey 1) escapism, 2) conformity, 3) amusements, 4) identification. 2. The expressive characteristics of Kitsch represent 1) aesthetic inadequacy: hair style and head dress showing the imperpectness, accumulation(superfluous ornaments), vulgarity, untidines. 2) resistance: hair style and head dress featuring deconstructional and violent forms revealing the uneasiness, and unsatisfaction of the given regularity. 3) humor: hair style and head dress featuring the innocence, humor, and fun for a relaxation and diversion. 4) hedonism: hair style and head dress with the erotic and sexual feelings. 5) retro, romantism: hair style and head dress featuring the elegance, femininity, folklore, ethnic, and nostalgia.

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Developing a Best-Evidence Pre-employment Medical Examination: An Example from the Construction Industry

  • Gouttebarge, Vincent;van der Molen, Henk F.;Frings-Dresen, Monique H.W.;Sluiter, Judith K.
    • Safety and Health at Work
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    • v.5 no.3
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    • pp.165-167
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    • 2014
  • The Dutch construction industry has introduced a compulsory preemployment medical examination (PE-ME). Best-evidence contents related to specific job demands are, however, lacking and need to be gathered. After the identification of job demands and health problems in the construction industry (systematic literature search and expert meeting), specific job demands and related requirements were defined and instruments proposed. Finally, a work ability assessment was linked to the instruments' outcomes, resulting in the modular character of the developed PE-ME. Twenty-two specific job demands for all Dutch construction jobs were identified, including kneeling/squatting, working under time pressure, and exposure to hazardous substances. The next step was proposing self-report questions, screening questionnaires, clinical tests, and/or performance-based tests, leading to a work ability judgment. "Lifting/carrying" is described as an example. The new modular PE-ME enables a job-specific assessment of work ability to be made for more than 100 jobs in the Dutch construction industry.

Notes on the Aloe Vera Aphid, Aloephagus myersi Essig (Hemiptera: Aphididae) on Non-native Aloe Plants in Korea (우리나라 비자생 알로에식물의 알로에진딧물 (노린재목, 진딧물과)에 대한 보고)

  • Song, Jeong-Heub;Suh, Soo-Jung
    • Korean journal of applied entomology
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    • v.53 no.3
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    • pp.317-319
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    • 2014
  • The aloe vera aphid, Aloephagus myersi Essig, was collected on imported Aloe vera (L.) Burm.f. (Xanthorrhoeaceae) grown in greenhouses in Korea. This species is native to tropical Africa and probably was introduced into greenhouses via the aloe plant trade. This introduction reinforces the need to focus attention on the eradication from collected localities, and for constant surveillance and detection at ports of entry to prevent introduction and establishment of new pests in the Korean environment. In this paper, additional information for the aloe vera aphid is provided with diagnoses and photographs along with host plant and distribution data for accurate species identification.

Protection of Consumer Privacy in Electronic Commerce -Focused on On-line Monitoring Survey of Electronic Commerce Firms- (전자상거래에 있어서 소비자 프라이버시 보호에 관한 연구 -인터넷 전자상거래업체의 온라인모니터링조사를 중심으로-)

  • Kim, Hea-Seon;Seo, Min-Kyo;Chun, Cheong-Ghi
    • International Commerce and Information Review
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    • v.6 no.2
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    • pp.219-242
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    • 2004
  • This study is conducted to find out whether companies which use electronic commerce for their business are collecting the least personal information. of consumers that are necessary for providing electronic commerce services to consumers. Investigators visited website of 799 electronic commerce companies, and checked out the personal informations of consumers that were asked when they join the site as a member. The collected data were analyzed with frequency, percentage, and cross-tab analysis using SPSS program. Generally most electronic commerces required more personal information of consumers than necessary for providing their services to consumers. These phenomena are partly due to the fact that regulations regarding consumer privacy in electronic commerce are advisory rather than mandatory at present. Therefore, it is suggested that mandatory regulation which makes companies poot their identification within a certain area of their web page has to enforced for the protection of consumer privacy in electronic commerce. And it would be more efficient if consumers are consistently educated about self protection guideline regarding personal information in using electronic commerce in addition to a mandatory regulation.

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Comparison of Stress Levels Arising in Clinical Settings, Stress Coping Mechanisms and Clinical Competency between Male and Female Nursing Students (남녀 간호대학생의 임상실습 스트레스, 대처방식 및 임상수행능력 비교)

  • Kim, Sung-Mi;Hwang, Tae-Yoon
    • The Journal of Korean Academic Society of Nursing Education
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    • v.20 no.4
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    • pp.606-616
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    • 2014
  • Purpose: This study compares differences between male and female nursing students in terms of the level of stress experienced during clinical practice settings, stress coping mechanisms and clinical competency. Method: The study population was nursing students who had taken a clinical practice course at one of five general hospitals in city D. Data were collected from September 15 to October 15, 2013 through a structured self-administered questionnaire survey given to 319 subjects (156 male students, 163 female students) enrolled in a statistical analysis course. Results: Male nursing students experienced lower stress levels during clinical practice and higher clinical competency than female students. In terms of stress coping mechanisms, male students were significantly more active than female students in deploying coping strategies. Among all students, students with higher clinical practice stress and concomitant stress coping scores exhibited higher clinical competency. Conclusion: Noticeable differences between male and female nursing students in terms of stress levels arising in the course of clinical practice, stress coping strategies, and clinical competency were revealed. However, further identification of specific stressful situations for male and female nursing students and subsequent management of clinical training circumstances are required.

A design of neuro-fuzzy adaptive controller using a reference model following function (기준 모델 추종 기능을 이용한 뉴로-퍼지 적응 제어기 설계)

  • Lee, Young-Seog;Ryoo, Dong-Wan;Seo, Bo-Hyeok
    • Journal of Institute of Control, Robotics and Systems
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    • v.4 no.2
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    • pp.203-208
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    • 1998
  • This paper presents an adaptive fuzzy controller using an neural network and adaptation algorithm. Reference-model following neuro-fuzzy controller(RMFNFC) is invesgated in order to overcome the difficulty of rule selecting and defects of the membership function in the general fuzzy logic controller(FLC). RMFNFC is developed to tune various parameter of the fuzzy controller which is used for the discrete nonlinear system control. RMFNFC is trained with the identification information and control closed loop error. A closed loop error is used for design criteria of a fuzzy controller which characterizes and quantize the control performance required in the overall control system. A control system is trained up the controller with the variation of the system obtained from the identifier and closed loop error. Numerical examples are presented to control of the discrete nonlinear system. Simulation results show the effectiveness of the proposed controller.

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Identification of Dinitrotoluene Selective Peptides by Phage Display Cloning

  • Jang, Hyeon-Jun;Na, Jung-Hyun;Jin, Bong-Suk;Lee, Won-Kyu;Lee, Woong-Hee;Jung, Hyun-Jin;Kim, Seok-Chan;Lim, Si-Hyung;Yu, Yeon-Gyu
    • Bulletin of the Korean Chemical Society
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    • v.31 no.12
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    • pp.3703-3706
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    • 2010
  • Biomolecules specific to explosives can be exploited as chemical sensors. Peptides specific to immobilized dinitrotoluene (DNT) were identified using a phage display library. A derivative of DNT that contained an extended amine group, 4-(2,4-dinitrophenyl)butan-1-amine, was synthesized and immobilized using a self-assembled monolayer surface on gold. Filamentous M13 phages displaying random sequences of 12-mer peptides specific to the immobilized DNT-derivate were isolated from the M13 phage library by biopanning. A common peptide sequence was identified from the isolated phages and the synthesized peptides showed selective binding to DNT. When the peptide was immobilized on a quartz crystal microbalance (QCM) chip, it showed a binding signal to DNT, while toluene barely showed significant binding to the QCM chip. These results demonstrate that peptides screened by biopanning against immobilized DNT can be useful for quick and accurate detection of DNT.