Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Isolation and identification of Flavobcterium succinicans from anadromous ayu Plecoglossus altivelis

소하은어의 병소로부터 Flavobcterium succinicans의 분리 및 동정

  • Lee, Chang-Hoon (Jeju Fisheries Institute, NFRDI) ;
  • Kim, Pil-Youn (Jeju Special Self-Governing Province, Ocean and Fisheries Research Institute) ;
  • Lim, Bong-Soo (Marine and Environmental Research Institute, Jeju National University) ;
  • Oh, Duck-Chul (College of Natural Sciences, Jeju National University) ;
  • Kang, Bong-Jo (College of Natural Sciences, Jeju National University)
  • 이창훈 (국립수산과학원 제주수산연구소) ;
  • 김필연 (제주특별자치도 해양수산연구원) ;
  • 임봉수 (제주대학교 해양과환경연구소) ;
  • 오덕철 (제주대학교 자연과학대학) ;
  • 강봉조 (제주특별자치도 해양수산연구원)
  • Published : 2009.12.01
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Abstract

On May in 2008, mortality of anadromous Ayu Plecoglossus altivelis was observed on the Gangjeong river in Jeju. Major symptoms of the infected fish were mouth rot and skin ulcer. The causative agent was suspected as gliding bacteria. After culture on Shu-Shott and R2A media, we isolated bacterium belonging to the Flavobacterium from ayu with symptoms. As a result, the bacterium was identified as Flavobacterium succinicans JMFL55 by 16S rDNA sequence alignment with F. succinicans DSM 4002(98.27% similarity, GenBank accession NO. AM230492).

2008년 5월 제주지역 하천에 소하하는 은어 중 일부개체가 주둥이 부식 또는 체표궤양증상을 보이면서 폐사하였다. 어류 질병원인균 중 부식증 및 체표궤양과 연관성이 높은 Flavobac-terium 속 세균의 분리를 위해 Shu-Shott medium 및 R2A medium에 주둥이 부식 및 체표궤양 부위를 도말하여 배양 하였고, Flavobacterium 속 세균의 전형적인 집락인 pale yellow colony를 분리하였다. 16S rDNA 염기서열 분석결과 Flavobacterium succinicans DSM 4002(Accession NO. AM230492)와 98.27%의 상동성을 보여 F. succinicans JMFL55로 동정하였다.

Keywords

References

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