• Archives of Pharmacal Research
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• 제15권1호
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• pp.41-47
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• 1992

In order to obtain acetaminophen, a popular analgesic-antipyretic, though microbial p-hydroxylation and N-acetylation of aniline, various Streptomyces strains were screened. Aniline N-acetylation activity was rather ubiquitous but-hydroxylation activity was selective. Microbial conversion pathway of aniline to acetaminophen was considered to be through N-acetylation and p-hydroxylation or vice versa. However, depending on species used, o-hydroxylation and its degradation activity (S. fradiae) and acetaminophen degradation activity (S. coelicolar) were also detected. Among the screened Streptomyces strains, S fradiae NRRL 2702 showed the highest acetanilide p-hydroxylation activity (203% conversion rate). Furthermore, in S. fradiae carbon source and its concentration, phosphate ion concentration and pH of growth medium were found to play the crucial roles in p-hydroxylation activity. Through the proper combination of factors mentioned above, the ten times more activity (26-30% conversion rate) was attained.

• 한국미생물·생명공학회지
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• 제22권3호
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• pp.329-331
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• 1994

For effective screening research, to isolate many different strains not duplicated would be essential. Three kinds of media were used for selective isolation of actinomycetes to test the reisolation frequency. Sixty species were isolated on Bennet's agar, 47 were on glycerol-asparagine agar and 77 were on humic acid-vitamin agar from 10 domestic soil samples. All of these isolates were identified to the genus level based on morphological characters and examined the sameness on each medium. The reisolation frequency between two different media was 10% or so(8.3~16.7%) and among three different media was 25% or so(22.1~27.7%).

• 한국연소학회:학술대회논문집
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• 한국연소학회 2006년도 제32회 KOSCO SYMPOSIUM 논문집
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• pp.49-54
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• 2006

The present study conducted a numerical modeling on the diesel SCR (selective catalytic reduction) system using ammonia as a reductant over vanadium-based catalysts $(V_2O_5-WO_3/TiO_2)$. Transient modeling for ammonia adsorption/desorption on the catalyst surface was firstly carried out, and then the SCR reaction was modeled considering for it. In the current catalytic reaction model, we extended the pure chemical kinetic model based on laboratory-scale powdered-phase catalyst experiments to the chemico-physical one applicable to realistic commercial SCR reactors. To simulate multi-dimensional heat and mass transfer phenomena, the SCR reactor was modeled in two dimensional, axisymmetric domain using porous medium approach. Also, since diesel engines operate in transient mode, the present study employed an unsteady model. In addition, throughout simulations using the developed code, effects of space velocity on the DeNOx performance were investigated.

• 대한가정학회지
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• 제33권2호
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• pp.151-157
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• 1995

This is a study on types of foodservice system, the menu, role of the Dietitian, management of the facilities and utensil through the survey conducted at 105 enterprises located in Seoul industrial foodservices which were evenly divided into three groups ; large size of feeding group, medium size of feeding group, small size of feeding group. The sresults are below, 1. Most institutional foodservice was enterprise under direct management. 2. Selective menu was set for the large size of feeding group than small size of feeding group. At budget making, personal management and purchansing management, the dietition role was not important in the large size of feeding group than in the small size of feeding group. 3. Equipped rate of facilities and utensil were greater volume for the large size of feeding group.

• Journal of Microbiology
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• 제35권1호
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• pp.10-14
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• 1997

The database of metabolic fingerprints generated using the GIolog system of lactic acid bacteria isolated from kimchi, described by Lee et al. (8), was used for the identification of 75 Leuconostoc isolates. The test strains were isoalted using a selective isolation medium specific for the genus Leuconostoc and examined for their ability to oxidize carbon sources using the Biolog system. The results show that the 75 test strains were identified to the known Leuconostoce clusters. It is suggested that the Biolog system can be applied for rapid identification of lactic acid bacteria isolated from kimchi.

• 약학회지
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• 제40권3호
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• pp.343-346
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• 1996

The influence of LB20304a, a new fluoroquinolone antibiotic agent, on microflora of caecum in mice was compared with those of ciprofloxacin and piperacillin after administration of drugs for 5 days. Selective medium (CCFMA) was used for the isolation of Clostridium difficile from the specimens of mouse caecum. The emergence of C. difficile in mouse caecum induced by LB20304a was lower than that by ciprofloxacin or piperacillin at day 1 and day 7 after completing administration of drugs.

• 한국환경보건학회지
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• 제30권2호
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• pp.115-122
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• 2004

A glassy carbon electrode(GCE) modified with nafion-DTPA (diethylene triamine-pentaacetic acid)-glycerol is used for the highly selective and sensitive determination of a trace amount of Cu(II). Various experimental parameters, which influenced the response of nafion-DTPA-glycerol modified electrode to Cu(II), are optimized. The Copper(II) is accumulated on the electrode surface by the formation of the complex in an open circuit, and the resulting surface is characterized by medium exchange, electrochemical reduction, and differential pulse voltammetry(DPV). The electrochemical response is evaluated with respect to concentration of modifier, pH and preconcentration time, quiet time, copper(II) concentration, and other variables. A linear range is obtained in the concentration range 1.0${\times}$10$^{-8}$ M-1.0${\times}$10$^{-6}$ MCu(II) with 7 min preconcentration time. The detection limit(3s) is as low as 2.36${\times}$10$^{-8}$ M (1.50 ppb).

• 미생물학회지
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• 제21권4호
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• pp.238-244
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• 1983

In order to obtain Ti plasmid which may be used in genetic engineering of higher plants, virulent Agrobacterium tumefaciens were isolated from soil with selective medium. For their identification, morphological and cultural characteristics were investigated in parallel with physiological and turnorigenesis test. 14 strains were isolated from various regions in Korea. 3 of them were identified as biotype 1 and the others were so heterogeneous that they are not yet conformed to any biotypes. However, Ti plasmid was isolated from A. tumefaciens KU 14 strain, one of the above mentioned 14 strains.

• 한국환경보건학회지
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• 제23권4호
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• pp.33-38
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• 1997

Four bacterial strains able to degrade dichlorobenzene as the sole source of carbon and energy were isolated from soil by selective enrichment culture, and among them, one isolation was the best in the cell growth and identified as Pseudomonas sp. DCB3 by its morphology and physiological properties. Cell growth dramatically increased in a minimal medium containing 500ppm of dichlorobenzene was not detected any more at 72 hours after cultivation. The optimal temperature and initial pH for the growth of the isolated strain were 30$\circ$C and 7.0, respectively. Cell growth was increased by supplementing $(NH_2)_2CO$ and 50 ppm yeast extract as additional nutrients. Therefore, it was suggested that Pseudomonas sp. DCB3 could be effectively used for the biological treatment of wastewater containing dichlorobenzene.

• 분석과학
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• 제17권6호
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• pp.476-480
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• 2004

A simple, rapid, reproducible and selective UV-Visible spectrophotometric method has been developed for determination of germanium in a week acid medium (pH = 3.0) in the presence of mandelic acid (MA) and malachite green (MG). A single extraction in chlorobenzene was used for the analysis. The germanium-MA ion association complex exhibits an absorption maximum at ${\lambda}_{max}=618nm$ with MG as counter ion and has an apparent molar absorptivtity of $1.313{\times}10^5L\;mol^{-1}\;cm^{-1}$. The values obtained by this UV-Visible spectrophotometric method are in good agreement with the values from inductively coupled plasma-mass spectrometer analysis.