• 제목/요약/키워드: Selective media

검색결과 332건 처리시간 0.037초

Performance of Self-Manufactured Ion Selective Microelectrode (ISME) for Continuous Monitoring of Ammonia and Nitrate Ions

  • Byun, Im-Gyu
    • 한국환경과학회지
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    • 제21권12호
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    • pp.1449-1454
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    • 2012
  • The ion selective microelectrodes (ISME) have been applied to observe the continuous profiles of NO3-N and NH4-N in bulk solutions or biofilms. In order to evaluate the performance and applicability of ion concentration measuring system, the characteristics, such as slope of calibration curve, detection limit and potentiometric selectivity coefficient were investigated. The slopes of calibration curve showed high degree of correspondence for each target ion concentrations. And the detection limits of nitrate and ammonia ion selective microelectrode were 10-4.7 M and 10-4.4 M, respectively. These ion selective microelectrodes were proved that their own performance could be maintained for 16 days after making. NO3-N and NH4-N selective microelectrodes were also adapted to detect the continuous ion profiles of cilia media packed MLE (Modified Ludzack-Ettinger) process. And the monitored nitrate and ammonia ion profiles with the ion selective microelectrode were stable and well corresponded to the results with conventional ion chromatograph. However, the electric potential was unstable until 8 hr because of the unknown noise. The tip shape and performance of the ion selective microelectrode was stably kept over 2 days continuous monitoring.

Comparison of Bifidobacteria Selective Media for the Detection of Bifidobacteria in Korean Commercial Fermented Milk Products

  • Kim, Eung-Ryool;Cho, Young-Hee;Kim, Yong-Hee;Park, Soon-Ok;Woo, Gun-Jo;Chun, Ho-Nam
    • 한국축산식품학회지
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    • 제30권1호
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    • pp.154-162
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    • 2010
  • This study was carried out to compare the efficacy and selectivity of TOS and BS media for enumeration of bifidobacteria in commercial fermented milk products. First, bifidobacteria was isolated from 20 fermented milk products, and all isolated bifidobacteria were identified by genomic technology as Bifidobacterium lactis. The two media significantly differed from each other with regard to the recovery of B. lactis, that is, the recovery of this organism was as much as 6 logs lower on BS medium than on TOS. When the concentration of BS solution (mixture of paromomycin sulfate, neomycin, sodium propionate, and lithium chloride) used in BS medium was reduced to 50% (BS50), a relatively high percentage recovery of bifidobacteria from pure cultures was achieved. Susceptibility tests to antibiotics and tests for selective agents for the isolated bifidobacteria and lactic acid bacteria were conducted. The BS solution inhibited some lactic acid bacteria and Bifidobacterium species, while mupirocin (MU) suppressed the growth of all tested lactic acid bacteria but not Bifidobacterium. As compared with BS50 medium, TOS with or without MU showed good bifidobacteria recovery and readily distinguishable colonies; in particular, TOS supplemented with MU had a high selectivity for bifidobacteria. In conclusion, all results suggested that TOS medium with or without MU was found to be suitable for selective enumeration of bifidobacteria from mixed cultures in fermented milk, and better in that capacity than BS medium.

Development of Modified Selective Media to Differentiate Cryptococcus Species Complex and its Serotypes using Natural Materials

  • Park, Gyu-Nam;Kim, Hye-Ran;An, Dong-Jun;Chae, Hee-Sun;Chang, Kyung-Soo
    • 대한의생명과학회지
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    • 제23권2호
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    • pp.64-72
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    • 2017
  • The formation of brown colonies due to phenol oxidase activity on classic agar media containing natural material extracts of Helianthus annuus or on medium containing L-3,4-dihydroxyphenylalanine has been used to identify Cryptococcus species complex. In this study, various natural materials were used to develop a modified medium and to identify five major serotypes of Cryptococcus species complex. Serotypes A, D, and A/D were pigmented on medium using Perilla frutescens var. japonica Hara (PerJ agar) after a three-day incubation. Serotypes B and C were pigmented on PerJ agar after four- and five-day incubations, respectively. Growth time and pigmentation of the five serotypes occurred more rapidly on PerJ agar than on the other media. In addition, colony morphology, size, and pigmentation were specific by serotype. In conclusion, PerJ agar should be used in clinic settings to identify Cryptococcus species complex and its serotypes rapidly.

Selective colonization and removal of senescent flowers of zucchini squash by Trichoderma hrzianum YC459, a biocontrol agent for gray mold, Botrytis cinerea

  • Kim, Geun-Gon;Chung, Young-Ryun
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.90.2-91
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    • 2003
  • In commercial greenhouses, senescent flower petals or flowers of vegetables such as tomato, strawberry, hot pepper and zucchini squash were blighted to be removed from fruits within five days after spraying of Trichoderma harzianum YC459 (TORY), a biocontrol agent for the gray mold rot of vegetables caused by B. cinerea The mechanism for selective colonization of senescent floral tissues by T. harzianum YC459 was elucidated using fresh and senescent (Hays and 14days after flowering, respectively) floral tissues of zucchini squash (Cucurbita moschata Duchesne). The spores of T. hrzianum YC459 were produced more on agar and liquid culture media supplemented with 5% dry powder of senescent floral tissues than fresh tissues during 15days. Mycelial growth was also much better in the media with senescent tissues than with fresh tissues. Enzyme activities of amylase, polygalacturonase and cellulase in the liquid media which might be involved in the colonization of tissues by T. harzianum YC459 were compared. The activities of three enzymes were much higher in the media with senescent floral tissues than with fresh floral tissues reaching to the maximum during 9 to 12days of incubation. Based on the results, the removal of senescent floral tissues, a possible inoculum source of the pathogen, may be another mechanism for biocontrol of gray mold rot of vegetables by T. harzianum YC459.

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Salmonella균(菌) 분리용(分離用) 증균배지(增菌培地)의 비교실험(比較實驗) (A Comparison of three Enrichment Media for Isolating Salmonella)

  • 김영자;이승윤;박기덕;민창홍
    • 대한미생물학회지
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    • 제11권1호
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    • pp.33-48
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    • 1976
  • The practical significance of using a selective enrichment procedure for detecion and enumeration of salmonella is well recognized. There are still various selective enrichment media has been communly used. Early years selenite broth was recomnended as an enrichment media for the isolating of salmonella. Hajna introduced a modified tetrathionate broth and demonstrated the greater efficiency to compare with the previous enrichment media. Raj also described that the new medium called dulcitol selenite enrichment and has been found to be very satisfactory, especially general implication in food poisoning. Authors tried to compare these 3 enrichment media for isolating salmonella. 1. When salmonella strains were inoculated $1{\sim}10^6$ cells per tube to these 3 enrichment media, mostly similar results were obtained between selenite broth and DS broth. In these 2 enrichment broth were showed $10^7/ml-10^8/ml$ cells of all tested salmonella strains. But in the case of TT broth it was found that the growth was $10^3/ml{\sim}10^4/ml$ cells for tested strain. 2. When E. coli, Proteus, Citrobacter were inoculate $10{\sim}10^6$ cells per tube to these 3 enrichment media. It was suggested that DS broth was showed more inhibitory action than that of selenite broth. TT broth showed high inhibition to these 3 organisms tested. 3. It was generally known that the incubation time is influenced to the frequency of salmonella detection. For this tendency, DS broth and selenite broth were showed similar results within 24 hrs to 48hrs incubation to the test. But DS broth showed more inhibitory action to E. coli and Proteus than that of selenite broth. 4. When $1{\sim}10$ cells were inoculated(per tube) to these 3 enrichment media, DS broth was found to be more sensitive than that of selenite broth.

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고추 탄저병균Colletotrichum acutatum 의 포장 밀도 조사를 위한 반선택 배지의 확립 및 활용 (Semi-selective Medium for Monitoring Colletotrichum acutatum Causing Pepper Anthracnose in the Field)

  • 강범관;민지영;김윤식;박성우;;김흥태
    • 식물병연구
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    • 제11권1호
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    • pp.21-27
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    • 2005
  • 고추 탄저병균인(Colletotrichum acutatum)은 100$\mu\textrm{g}$ / $m\ell$ 의 ampicillin과 tetracycline과 같은 항생물질과 100$\mu\textrm{g}$ / $m\ell$의 carbendazim과 diethofencarb의 혼합체를 첨가한 PDA 배지에서 특이적으로 생장하였다. 탄저병의 발병 정도가 다른 고추 열매르 ㄹ갈아서 C.acutatum의 반선택 배지에 도말한 결과, 발병 정도와 반선택 배지에 나타나는 병원균의 균총 수는 정의 상관 관계를 나타냈다. 고추를 재배했던 포장에서 이병 잔재물과 토양을 채집하여 반선택 배지 상에서 병원균의 수를 조사한 결과, C.acutatum은 토양보다는 이병 잔재물에서 더 많은 수가 검출되었다. 반선택 배지에 나타난 병원균을 무작위로 10균주를 선발하여 동정하였다. 선발한 균주들의 DNA를 추출하여 C. acutatum 특이적인 printer를 사용한 PCR 을 수행한 결과, 모든 균주가 C. acutatum으로 확인되었다.

A Selective Protection Scheme for Scalable Video Coding Based on Dependency Graph Model

  • ;김문철
    • 한국방송∙미디어공학회:학술대회논문집
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    • 한국방송공학회 2010년도 추계학술대회
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    • pp.78-81
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    • 2010
  • In this paper, we propose an efficient and effective selective protection scheme to SVC that exploit the propagation of protection effect by protecting significant frames that can give the maximum visual quality degradation. We model SVC dependency coding structure as a directed acyclic graph which is characterized with an estimated visual quality value as the attribute at each node. The estimated visual quality is calculated by using our model based on the proportions of intra- and inter-predicted MBs, amounts of residual, and estimated visual quality of reference frames. The proposed selective protection scheme traverses the graph to find optimal protection paths that can give maximum visual quality degradation. Experimental results show that the proposed selective protection scheme reduces the required number of frames to be protected by 46.02% compared to the whole protection scheme and 27.56% compared to the layered protection scheme.

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An Optimal Selective Protection Scheme for Scalable Video Coding

  • 헨드리;김문철
    • 한국방송∙미디어공학회:학술대회논문집
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    • 한국방송공학회 2011년도 하계학술대회
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    • pp.294-295
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    • 2011
  • The fundamental problem of selective protection scheme for encoded bitstreams is to find an efficient algorithm to select the set of frames required to be encrypted that can maximize protection effect with the minimum amount of protected data is required. In this paper, we propose an optimal selective protection scheme for SVC bitstreams by protecting the best combination of frames for selective protection in the sense that the amount of data required for protection is minimized and the resulting visual quality degradation is maximized. The selection of the frames to be encrypted is done by first expressing R-Q (protection rate - visual quality) relationship with Lagragian cost model. The experimental results show that, compared to protecting SVC bitstreams layer by layer, the proposed scheme gives superior performance in terms of protection effectiveness due to its better selection of frames for protection given protection bit budgets.

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Non-O157 장출혈성대장균 검출을 위한 증균배지 및 선택배지 성능 평가 (Evaluation of enrichment broth and selective media for the detection of non-O157 enterohemorrhagic Escherichia coli)

  • 이다연;김희언;서동원;조용선
    • 한국식품과학회지
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    • 제48권4호
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    • pp.323-328
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    • 2016
  • 본 연구는 non-O157 EHEC의 혈청형별 증균 및 선택배지에서의 분리 조건 및 비교 시험을 하여 최적의 검출 방법을 제시하고자 하였다. EC medium with MUG broth, BRILA broth, mTSB broth with novobiocin 3종의 증균배지 성능 및 배양 조건의 비교 결과 EC-MUG 증균배지를 $42^{\circ}C$에서 24시간 배양하면 EHEC의 효율적인 증균이 이루어질 수 있을 것이라 생각된다. ENDO agar, Chromocult agar, TBX agar, CHROMagar$^{TM}$ STEC medium 4종의 선택배지에 가장 효과적인 배지를 확인결과 TBX agar가 검출율이 가장 높았으나 1종의 배지를 사용했을 경우 다양한 EHEC 혈청형 균를 검출 하기는 어렵다고 생각된다. 그러므로 EHEC를 효과적으로 검출하고자 할 경우 TBX agar와 보완이 가능한 그 외의 배지를 사용하여야 효과적인 검출이 가능할 것으로 생각된다. 또한, 각 배지가 혈청형에 따른 분리효율이 다르므로 본 연구 결과를 참조하여 가장 적합한 배지를 선택 해야 할 것으로 보인다. 향후 non-O157 EHEC의 적절한 기질을 사용하여 다양한 혈청형을 모두 검출 할 수 있는 효율적인 배지의 개발이 필요하다고 생각된다.

Antecedents of News Consumers' Perceived Information Overload and News Consumption Pattern in the USA

  • Lee, Sun Kyong;Kim, Kyun Soo;Koh, Joon
    • International Journal of Contents
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    • 제12권3호
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    • pp.1-11
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    • 2016
  • This exploratory study examines the critical factors associated with news consumers' perception of information overload and news consumption patterns. An online survey was conducted with Qualtrics panels (N = 1001). The demographics and three antecedent factors of perceived information overload were considered including the frequency of news access through multiple media platforms, level of attention to news, and interest in news. Three news consumption patterns were investigated as possible consequences of perceived information overload: news avoidance, selective exposure, and willingness to pay for news. The results of hierarchical regression analyses revealed a meaningful distinction between general and news information overload. Overall, news consumers who paid more attention to news through newer media/platforms/devices perceived higher levels of information overload, were more willing to pay for the news, and often avoided news or selectively exposed themselves to certain sources of news to manage news information overload.