• Pediatric Infection and Vaccine
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• 제13권2호
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• pp.201-205
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• 2006

원발성 면역 결핍증에는 항체형성에 영향을 주는 질환이 가장 흔하고 그중 선택적으로 분비성 IgA 결핍이 많으나 IgG 농도가 정상 혹은 증가되어 있는 어떤 환자에서는 4가지의 IgG 아형 중에서 한 두 개 이상의 결핍이 발견된다. 대부분의 환자, 특히 IgG4 결핍증 환자에서 폐구균, 포도구균, 헤모필루스균 등에 의한 부비동염, 중이염, 폐렴 등이 반복된다. 상기 10세 여아는 평소에 잦은 상기도 감염을 앓아 왔고, 결핵성 림프절염으로 약물요법을 시행한 병력이 있으며, 매년 2~3차례 폐렴으로 입원치료를 받아왔다. 키와 몸무게가 모두 3백분위수 미만으로 성장부전(growth failure)가 지속되었다. 2003년 9월 심한 폐렴으로 입원 치료 중에 면역결핍 검사를 시행하였다. IgG, IgM level은 정상이었으나 IgG subclass 2, 3, 4에서 모두 감소된 수치를 보여 IgG 아형 결핍을 보였다. 저자들은 반복적인 호흡기 질환을 앓는 환아에서 성장부전이 동반된 IgG 아형 결핍 1례를 보고하는 바이다.

• 식물병연구
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• 제15권2호
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• pp.77-82
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• 2009

최근 혹병이 심하게 발생한 충북지방의 포도나무의 뿌리로부터 선택배지를 이용하여 Agrobacterium속 세균을 분리하고 동정하였다. 분리균의 지방산 분석을 통한 MIDI에 의한 동정, 16S rDNA 염기서열, 생화학적 특성, 종 특이적 primer을 이용한 PCR 결과로 13개 분리균은 모두 A. tumefaciens로 동정 되었으며, 포도나무 혹병균인 A. vitis는 분리되지 않았다. 모든 분리균은 토마토와 포도나무의 줄기와 뿌리에 병원성을 나타내지 않았다. Rep-PCR 결과 분리균은 A. tumefaciens와 일부 유사성이 있지만 전체적으로 병원균인 A. tumefaciens와 A. vitis와는 유사성이 낮았다. 이상의 결과는 충북 일부 지방의 MBA와 거봉에서 발생한 혹병을 일으키는 병원균은 토양이나 뿌리로부터 전반되지 않고 묘목을 통해서 전반되었을 가능성을 시사하고 있다.

• 한국동물위생학회지
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• 제32권2호
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• pp.147-153
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• 2009

Salmonella species are the most important etiologic agents of food-borne acute gastroenteritis. The most common serotypes isolated from humans are Salmonella enterica serotype Typhimurium (S. Typhimurium) and S. Enteritidis. Traditional detection methods for Salmonella are based on cultures using selective media and characterization of suspicious colonies by biochemical and serological tests. These methods are generally time-consuming and not so highly sensitive. Recently, the polymerase chain reaction (PCR) has been used as a highly sensitive, specific, and rapid test for the presence of pathogenic bacteria. In this study, a multiplex PCR (m-PCR) was used to detect S. Typhimurium and S. Enteritidis. We selected m-PCR target genes, which were the spv (virulence plasmid specific for S. Enteritidis) and sefA (S. Enteritidis fimbrial antigen) genes, fliC (H1-i antigen specific for S. Typhimurium) and a randomly cloned sequence specific for the genus Salmonella. With m-PCR, random sequence was detected from all strains of Salmonella spp, spv and sefA were detected from all strains of S. Enteritidis (100%), and fliC was detected from all strains of S. Typhimurium (100%). This assay indicate that the specificity of the m-PCR make them potentially valuable tools for detection of S. Typhimurium and S. Enteritidis.

• Journal of Animal Science and Technology
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• 제46권6호
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• pp.999-1006
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• 2004

NPC agar， DHL agar, MacConkey agar와 Cetrimide desoxycholate agar의 선택성을 조사 하였다". Cetrimide desoxycholate agarz 가 다른 선택성 배지에 비해 그람양성 세균을 억제하고 Pseudomonas를 비롯한 내냉성 그람음성 세균의 선택적 배양에 더 좋은 한천배지이었다. Resazurin 환원시간 검시에서 Cetrimide desoxycholate broth를 시유와 혼합하여 $21^{\circ}C$ 에 서 18 시간 배양한 후 resazurin을 첨가하여 변색되는 시간으로 2차오염 을 결정하는 방법으로서 조사하였다. Resazurin 환원시간 검사와 저장성 검사간에 81%의 관련성이 있었다. 저장성 검사의 우유에서 Pseudomonas가 가장 먼도 높게 분리되었으며 다음으로 Acinetobacter와 Aeromonas가 검출되었다 Resazurin 환원시간 검사에는 Acinetobacter, Pseudomonas, Enterobacter의 순서의 빈도로 검출되었다.

• 분석과학
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• 제29권5호
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• pp.225-233
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• 2016

입자 표면에 고분자를 화학적으로 접목하는 것은 수용성 액체 이상 시스템(ATPS)과 같이 분리된 액체 층에서 마이크로/나노입자를 선택적으로 분배시키는데 중요한 구실을 한다. 본 연구에서는 덱스트란, 폴리에틸렌 글리콜, 알부민을 화학적으로 폴리스티렌 자성 마이크로입자 표면에 화학적으로 부착시켰다. 분배 현상을 역전시킬 수 있는 긴 고분자 사슬의 부착은 일차 아민 작용기를 가진 다양한 고분자를 p-톨루엔술폰산(토실)기를 부착시킨 폴리스티렌 자성 마이크로입자와의 S_N2 치환에 의해 간단히 수행될 수 있었다. 이 후 적외선 현미경을 사용하여 마이크로입자의 표면 변화의 유무를 검사하였다. 반응 후 입자들은 세 가지 폴리머 모두 N-H 신축 진동 피크를 보였으며 대부분의 주요 피크들의 위치는 반응 전후에 유사하였으나 지문 영역에서 구별 가능한 차이를 보였다.

• 한국자원식물학회지
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• 제19권6호
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• pp.706-715
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• 2006

Nitrate reductase deficient (NR) mutant lines were selected indirectly by their resistance to 100mM chlorate in cell cultures of P. parviflora. A total of 585 chlorate resistant lines were confirmed by a second passage on a high concentration of chlorate. Frequency of spontaneous mutation was $9.7{\times}10^{-7}$ in 3 month old suspension-cultured cells, and in non-selective media containing amino acids as sole nitrogen source. The frequency of mutation could be increased up to 11-fold by culture for 12 months. Out of 40 randomly selected calli, 22 were fully deficient in NR. The rest of the clones contained a decreased level of NR activity. Further characterization was carried out in 13 mutant lines which were fully deficient in NR and in 5 mutant lines containing residual (0-7.0%) NR activity, as compared to wild-type cells cultured on the same medium. The $NR^-$ mutants were tentatively classified as defective in the NR apoenzyme (nia-type; 11 mutant lines including the 5 with residual NR activity) or in the molybdenum cofactor (cnx-type; 7 mutant lines) by the XDH activity. The cnx-type could be further classified into two groups. In one group (5 mutant lines) of these, the NR activity could be partially restored by nonphysiologically high (1.0mM) molybdate in the culture medium. Both types of $NR^-$ mutants were unable to grow on minimal medium containing nitrate as sole nitrogen source, but grew well on amino acids. They also proved to be extremely sensitive to the standard medium ($MSP_1$) containing nitrate and ammonium. Shoot regeneration was obtained only in the $NR^-$ mutants, which contained residual NR activity, but they so far have failed to grow into plants.

• 한국동물위생학회지
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• 제30권3호
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• pp.353-362
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• 2007

A total of 1,354 samples was collected from bovine and porcine carcass from January 2005 to December 2006 in a slaughter house. Twenty five strains(1.8%) of Listeria monocytogenes were isolated from 1,354 samples using selective media. Ten(1.4%) L monocytogenes were isolated from the 677 of bovine carcasses, and 15(2.2%) were isolated from the 677 of porcine carcasses. Among 15 L mono-cytogenes from porcine, 11 siolates were serovars 1/2c, followed by 1/2b (3 strains, 20.0%) and 1/2a(1 strain) Out of 10 bovine samples, positive cases in 1/2a were 9 strains (90.0%), 1/2b were 1 strains(10.0%). PCR primers were selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene (hlyA) of L mono-cytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested. A total of 25 L monocytogenes strains were analysed by PFGE after digestion with Apa I. PFGE analysis of genomic DNA showed the $14{\sim}18$ fragments ranging in size from 30 to 550 kb, resulting in 14 patterns.

• Journal of Oral Medicine and Pain
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• 제18권1호
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• pp.73-82
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• 1993

To investigate the changes in aerobic and facultative anaerobic oral microflora during remission-induction chemotherapy in patients with acute myeloid leukemia, 10 consecutive patients were studied during a period of 28 days. One day before, during and after the induction therapy, patients were given 10% Betadine solution for mouthrinses after breakfast and kept from eating and drinking. After 3 hours, paraffin-stimulated whole saliva was obtained for 2 minutes and transported to the laboratory. The samples were dispersed and homogenized by use of vortex mixer for 20 seconds. From these samples 10-fold serial dilutions (from 10-1 through 10-3) were prepared. Each dilution of 0.1 ml was plated on duplicate set of one nonselective medium (Blood agar) and four selective media (Sabourauds dextrose agar, Mannitol salt agar, Mac-Conkey agar, SF medium ) using applicator woods. All agar plate were incubated at 37$^{\circ}C$ for 48 hours. The total number of microorganisms was calculated and the percentage distribution of the various microorganisms from each specimen was drawn. 1. The salivary flow rate decreased by 66%, going from 5.38 ml/2min to 1.81 ml/2min over two days during the chemotherapy. 2. The total number of microorganisms in saliva increased by 22%, going from 4.88$\times$105/ml to 6.00$\times$105/ml over two days during the chemotherapy. 3. The salivary flow rate and the total number of microorganisms in saliva were recovered within 28 days after the chemotherapy. 4. The quantitative alteration in oral Enterobacteria, Enterococci, Staphylococci, Cndida during the chemotherapy had no statistical significance. 5. In saliva of the patients with acute myeloid leukemia who ahd intraoral ulcer, Enterobacteria was quantitatively predominent. Our study suggests that chemotherapy-induced transient xerostomia may induce acute oral infection. Consequently, the use of saliva substitute, the removal of intraoral infection source and the consistent oral hygiene care seem to be required to avoid the transmission of potential pathogenes in this group of patients.

• Journal of Microbiology and Biotechnology
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• 제22권12호
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• pp.1621-1628
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• 2012

The agar-degrading bacterium GNUM-1 was isolated from the brown algal species Sargassum serratifolium, which was obtained from the West Sea of Korea, by using the selective artificial seawater agar plate. The cells were Gram-negative, $0.5-0.6{\mu}m$ wide and $2.0-2.5{\mu}m$ long curved rods with a single polar flagellum, forming nonpigmented, circular, smooth colonies. Cells grew at $20^{\circ}C-37^{\circ}C$, between pH 5.0 and 9.0, and at 1-10% (w/v) NaCl. The DNA G+C content of the GNUM-1 strain was 45.5 mol%. The 16S rRNA sequence of the GNUM-1 was very similar to those of Alteromonas stellipolaris LMG 21861 (99.86% sequence homology) and Alteromonas addita $R10SW13^T$(99.64% sequence homology), which led us to assign it to the genus Alteromonas. It showed positive activities for agarase, amylase, gelatinase, alkaline phosphatase, esterase (C8), lipase (C14), leucine arylamidase, valine arylamidase, ${\alpha}$-chymotrypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, ${\alpha}$-galactosidase, ${\beta}$-galactosidase, ${\beta}$-glucosidase, catalase, and urease. It can utilize citrate, malic acid, and trisodium citrate. The major fatty acids were summed feature 3 (21.5%, comprising $C_{16:1}{\omega}7c/iso-C_{15:0}$ 2-OH) and C16:0 (15.04%). On the basis of the variations in many biochemical characteristics, GNUM-1 was considered as unique and thus was named Alteromonas sp. GNUM-1. It produced the highest agarase activity in modified ASW medium containing 0.4% sucrose, but lower activity in rich media despite superior growth, implying that agarase production is tightly regulated and repressed in a rich nutrient condition. The 30 kDa protein with agarase activity was identified by zymography, and this report serves as the very first account of such a protein in the genus Alteromonas.

• 한국정보과학회논문지:정보통신
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• 제37권6호
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• pp.483-488
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• 2010

모바일 기기와 네트워크 기술의 발달로 다양한 멀티미디어 응용 서비스가 빠르게 발전 및 보급되고 있다. 그리고, 사용자의 참여형 방송 서비스가 가능하며 이를 이용한 실시간 뉴스 등 다양한 정보 전달의 수단으로 이용되고 있다. 이러한 라이브 멀티미디어 스트리밍 서비스 사용자의 체감 품질을 만족시키기 위해서는 영상품질 이외에도 재생연속성과 재생지연한계를 고려해야 한다. 본 논문에서는 모바일 기기에서 실시간 스트리밍시 비디오 재생연속성과 재생지연한계를 보장하는 DeBuG (Delay Bounds Guaranteed) 전송률 조절 기법을 제안하였다. 제안한 기법유 실시간 비디오처리 시스템의 특성을 고려하여 송신 버퍼의 상태와 네트워크 상태를 인지하여 서비스 연속성과 지연한계를 보장하는 품질 적응 기능을 가지고 있으며, 비디오 영상의 품질을 보장하며 전달의 지연한계를 보장하기 위해 미디어의 프레임을 선택적으로 폐기하는 기능을 가지고 있다. 제안한 DeBuG 전송률 조절 기법의 주요 성능은 시뮬레이션을 통하여 보였다.