• Title/Summary/Keyword: Selective isolation

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Comparison of Muscle Activities between the Diagonal Pattern of Shoulder Exercises Using the %Normalization and %Isolation Method (독립화 비율과 정량화 비율을 사용한 대각선 패턴의 어깨운동 간의 근활성도 비교)

  • Sang-Yeol Lee;Se-Yeon Park
    • PNF and Movement
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    • v.21 no.1
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    • pp.87-94
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    • 2023
  • Purpose: The present study aims to investigate the effects of a diagonal exercise pattern on selective activation of the upper extremity muscles using both normalization and isolation methods. Methods: In total, 17 asymptomatic subjects participated in this study. During the two diagonal patterns of exercise (diagonals 1 and 2), muscular activities of the upper trapezius (UT), lower trapezius (LT), serratus anterior (SA), anterior deltoid (AD), and infraspinatus (IS) were measured. The collected data were analyzed in two ways, according to the dominance of muscle activities (%Isolation) and according to normalized activities (%MVIC). Results: There were significant differences in LT, SA, AD, and IS between %MVIC and %Isolation (p<0.05), and the diagonal 1 pattern of exercise showed significantly more LT activities compared with the diagonal 2 pattern (p<0.05). Further, except for LT, there were no significant differences in muscle activities between the diagonal 1 and 2 exercises. Conclusion: The present study suggests that a diagonal pattern of exercise is advantageous for strengthening shoulder muscles, but caution is needed when applying to patients requiring selective strengthening. Regarding both the concentric and eccentric phases of exercise, there was no significant difference in muscular activation, except in LT, between the two diagonal patterns of exercises.

Comparison of Selective Media for Isolation and Detection of Shigella spp. from Foods (식품으로부터 쉬겔라 검출을 위한 분리배지 비교)

  • In, Ye-Won;Ha, Su-Jeong;Oh, Se-Wook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.7
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    • pp.1025-1031
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    • 2011
  • The objective of this study was to compare the performances of conventional microbiological media used in isolation of Shigella spp. from foods. Total of six selective media, including MacConkey agar (MAC), Salmonella Shigella agar (SSA), desoxycholate citrate agar (DCA), xylose lysine desoxycholate agar (XLD), hektoen enteric agar (HEA), and CHROMagar, were tested. MAC showed almost the same colony numbers as compared to tryptic soy agar (TSA) while DCA showed significantly lower colony numbers when cultivated Shigella spp. was counted in each medium. In a food recovery test with beef, pork and shrimp, S. sonnei recovered well on CHROMagar (p<0.05). With lettuce and cabbage, S. sonnei displayed significantly significant recovery (p<0.05) on SSA in comparison with other selective media. Heat-injured cells recovered well on MAC and SSA. In a specificity test using Enterobacteriaceae strains, HEA was identified as having the highest specificity among the tested media. However, Morganella spp. could not be differentiated from Shigella spp. on any of the tested selective media. Shigella spp. precluded the possibility of isolation from foods by a single 'best' selective medium. Consequently, a combination of complementary selective media or selection of appropriate media according to cell conditions must be considered for comprehensive isolation.

A New Medium for the Selective Isolation of Soil Actinomycetes (토양중 방선균의 선택적 분리를 위한 배지)

  • Cho, Seong-Hag;Hwang, Cherl-Won;Chung, Ho-Kweon;Yang, Chang-Sul
    • Microbiology and Biotechnology Letters
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    • v.22 no.5
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    • pp.561-563
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    • 1994
  • For the more effective isolation of soil actinomycetes, we have developed HHV (Hair hydrolysate-vitamin) agar medium, containing hair as the sole source of carbon and nitrogen. The HHV agar medium was superior to other media such as colloidal chintin agar, glycerol-arginine agar and starch-casein-nitrate agar, and HV (humic acid-vitamin) agar. The maximum effect of this medium has been shown in hair dry weight 0.4 g/l medium. Of each soil sample, the greatestest number of actinomycetes was isolated from the potato annual planted soil among the tested samp- les. The genus of actinomycetes isolated from the potato annual planted soil sample was identified such 5 group as Stretomyces, Micromonospora, Microbispora, Nocardia and Saccharopolyspora.

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Wavelength Selective Switch using LCoS Display (LCoS 디스플레이를 이용한 파장선택스위치)

  • Lee, Yong-Min
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.8
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    • pp.5288-5293
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    • 2014
  • This paper presents the characterization of the wavelength selective switch using a LCOS display instead of a MEMS device, which is a core technology for the next generation ROADM. We constructed a five-port WSS, and examined the basic characteristics of the WSS, such as the response time, beam steering, insertion loss, and channel isolation. The response time of WSS was 11.6 mS and the beam steering characteristics of LCOS was well performed. The measured insertion loss at 40 channels in the c-band were 5.5~12.7 dB and channel isolation was 16~18 dB. Although the characteristics of LCOS-based WSS are inferior to the conventional MEMS-based WSS, it can be improved by additional experiments that secure the technology competitiveness.

Resistance of Fusarium fujikuroi Isolates to Hydrogen Peroxide and Its Application for Fungal Isolation

  • Youn, Kihoon;Choi, Hyo-Won;Shin, Dong Bum;Jung, Boknam;Lee, Jungkwan
    • Research in Plant Disease
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    • v.21 no.3
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    • pp.227-230
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    • 2015
  • The ascomycete fungus Fusarium fujikuroi causes bakanae disease in rice and this disease has been reemerging in Korea. Other fungal species including F. graminearum and Magnaporthe oryzae are often associated with F. fujikuroi, hampering pure isolation of F. fujikuroi from rice. In this study, we modified a selective medium for F. fujikuroi as supplementing both pentachloronitrobenzene and hydrogen peroxide into minimal medium. This medium efficiently suppressed the vegetative growth of F. graminearum and M. oryzae, but did not significantly reduce F. fujikuroi growth, providing an efficient tool for isolating F. fujikuroi.

Distribution of Alcohol-tolerant Microfungi in Paddy Field Soils

  • Choi, Soon-Young
    • Mycobiology
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    • v.31 no.4
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    • pp.191-195
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    • 2003
  • Ethanol treatment method was attempted for the selective isolation of ethanol-tolerant fungi from two sites of rice paddy fields around Seoul area. The vertical and seasonal fluctuation of the fungal population were also investigated. The ethanol-tolerant fungi were Talaromyces stipitatus, T. flavus var. flavus, T. helicus var. major, Eupenicillium javanicum, Emericellopsis terricolor, Pseudourotium zonatum, Aspergillus flavus, Cladosporium cladosporioides, Penicillium frequentans, P. janthinellum, and P. verruculosum. The most dominant species isolated by this method was T. stipitatus. It was found that the numbers of fungal species and colony forming units(CFUs) of ethanol-tolerant fungi were higher in Ascomycota than in Deuteromycota. A particular tendency appeared the highest CFUs in autumn, but lower in spring and winter. T. stipitatus was the dominant species of ethanol tolerant microfungi. This result would suggest that membrane lipid composition of ethanol-tolerant fungi isolated from the soils may play on important role in the ethanol tolerance.

A New Method for Deep Trench Isolation Using Selective Polycrystalline Silicon Growth (다결정 실리콘의 선택적 성장을 이용한 깊은 트랜치 격리기술)

  • 박찬우;김상훈;현영철;이승윤;심규환;강진영
    • Journal of the Korean Vacuum Society
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    • v.11 no.4
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    • pp.235-239
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    • 2002
  • A new method for deep trench isolation using selective growth of polycrystalline silicon is proposed. In this method, trench filling is performed by forming polysilicon-inner sidewalls within the trench, and then selectively growing them by reduced chemical vapor deposition using $SiH_2C1_2$gas at $1100^{\circ}C$. The surface profiles of filled trenches are determined mainly by the initial depth of inner sidewalls and the total thickness of selective growth. No chemical mechanical polishing(CMP) process is needed in this new method, which makes the process flow simpler and more reliable in comparison with the conventional method using CMP process.

Development of a Selective Medium for the Fungal Pathogen Fusarium graminearum Using Toxoflavin Produced by the Bacterial Pathogen Burkholderia glumae

  • Jung, Boknam;Lee, Sehee;Ha, Jiran;Park, Jong-Chul;Han, Sung-Sook;Hwang, Ingyu;Lee, Yin-Won;Lee, Jungkwan
    • The Plant Pathology Journal
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    • v.29 no.4
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    • pp.446-450
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    • 2013
  • The ascomycete fungus Fusarium graminearum is a major causal agent for Fusarium head blight in cereals and produces mycotoxins such as trichothecenes and zearalenone. Isolation of the fungal strains from air or cereals can be hampered by various other airborne fungal pathogens and saprophytic fungi. In this study, we developed a selective medium specific to F. graminearum using toxoflavin produced by the bacterial pathogen Burkholderia glumae. F. graminearum was resistant to toxoflavin, while other fungi were sensitive to this toxin. Supplementing toxoflavin into medium enhanced the isolation of F. graminearum from rice grains by suppressing the growth of saprophytic fungal species. In addition, a medium with or without toxoflavin exposed to wheat fields for 1 h had 84% or 25%, respectively, of colonies identified as F. graminearum. This selection medium provides an efficient tool for isolating F. graminearum, and can be adopted by research groups working on genetics and disease forecasting.

Comparative Evaluation of Selective Media for Isolation of Bifidobacterium Species in Human Fecal Sample (인체 분변에서 Bifidobacterium species의 선택적 분리를 위한 배지 비교)

  • Saeyoun Shin;Sejong Oh
    • Journal of Dairy Science and Biotechnology
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    • v.42 no.1
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    • pp.9-17
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    • 2024
  • This study aimed to evaluate the appropriateness of MRS-C (0.05% L-cystein; pH 5) and BHI-CM (0.05% L-cystein, 0.5% mucin) agars for the selective isolation of bifidobacteria in fecal samples compared to blood-liver-NPNL (BL-NPNL) agar. Over 200 isolated colonies were characterized morphologically and biochemically. Genomic DNA was extracted from pure cultures of the isolated strains, followed by PCR amplification of the 16S rRNA gene. Bifidobacterium longum and B. animalis were selectively isolated from MRS-C agar and Lactobacillus acidophilus and Enterococcus avium were also isolated. B. longum, B. faecale, and B. animalis were isolated from feces on BHI-CM agar; however, different Bacteroides strains (including Bac. fragilis, Bac. kiribbi, Bac. ovatus, Bac. koreensis, and Bac. salyersiae) were also detected. BL-NPNL agar successfully isolated B. longum and Bacillus, while other Bifidobacterium and Bacteroides species could not grow owing to the presence of antibiotics in the medium. The use of antibiotics in a medium can enhance the selectivity; however, antibiotics may inhibit the growth of certain bacteria in a sample. Hence, adjusting pH or adding non-antibiotic nutrients to the medium is more advantageous, than relying on antibiotics.

Isolation and Identification of Pathogenic Microorganisms from Soybean Sprouts

  • Kim, Hye-Jung;Koo, Kyoung-Mo;Kim, Gi-Nahm;Lee, Dong-Sun;Paik, Hyun-Dong
    • Preventive Nutrition and Food Science
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    • v.7 no.3
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    • pp.305-309
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    • 2002
  • Raw soybean sprouts were tested for contamination with the following bacteria which have potential for pathogenesis or food spoilage : Salmonella spp., Escherichia coli O157:H7, Yersinia enterocolitica, Vibrio parahae-molyticus, Aeromonas hydrophila, Plesidomonas shigeloides, Pseudomonas aeruginosa, Staphylococcus aureus, Lis-teria monocytogenes, Bacillus cereus, Clostridium perfringens, Campylobacter jejuni, Erwinia spp., and Fusarium spp. Three of the above strains were isolated from the sprouts, and identified by morphological and biochemical methods including an API kit and ATB automated identification system. The isolate cultured in Cereus selective agar, a selective medium, was a Gram-positive, rod shaped, anaerobic spore former. The biochemical and culture tests revealed the following characteristics: catalase-positive, no growth on Simmon's citrate, NO₂ production and requirement of arginine for growth; the ATB automated identification system gave 99.8 % agreement for the identification of Bacillus cereus to the species level. The isolate cultured in Macconkey agar selective medium was Gram-negative, rod shaped and a gas former; the ATB-system gave 99.9% agreement for the identification of Aeromonas hydrophila to the species level. The isolate found in Pseudomonas isolation agar was Gram-negative, rod shaped, cytochrome oxidase-positive, a reducer of nitrates to nitrogen, and pyocyanin producer; the ATB-system gave 99.9 % agreement for the identification of Pseudomonas aeruginosa to the species level. These results indicate that the three bacteria species present in the soybean sprouts were Bacillus cereus, Aero-monas hydrophila, and Pseudomonas aeruginosa. Salmonella spp., Escherichia coli O157:H7, and Yersinia enter-ocolitica, which are associated with serious disease in humans, were not isolated from soybean sprouts examined in this study.