The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of a Selective Medium for the Fungal Pathogen Fusarium graminearum Using Toxoflavin Produced by the Bacterial Pathogen Burkholderia glumae

  • Jung, Boknam (Department of Applied Biology, Dong-A University) ;
  • Lee, Sehee (Department of Applied Biology, Dong-A University) ;
  • Ha, Jiran (Department of Applied Biology, Dong-A University) ;
  • Park, Jong-Chul (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration) ;
  • Han, Sung-Sook (Crop Environment Division, National Institute of Crop Science, Rural Development Administration) ;
  • Hwang, Ingyu (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Yin-Won (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Jungkwan (Department of Applied Biology, Dong-A University)
  • Received : 2013.07.14
  • Accepted : 2013.09.24
  • Published : 2013.12.01
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Abstract

The ascomycete fungus Fusarium graminearum is a major causal agent for Fusarium head blight in cereals and produces mycotoxins such as trichothecenes and zearalenone. Isolation of the fungal strains from air or cereals can be hampered by various other airborne fungal pathogens and saprophytic fungi. In this study, we developed a selective medium specific to F. graminearum using toxoflavin produced by the bacterial pathogen Burkholderia glumae. F. graminearum was resistant to toxoflavin, while other fungi were sensitive to this toxin. Supplementing toxoflavin into medium enhanced the isolation of F. graminearum from rice grains by suppressing the growth of saprophytic fungal species. In addition, a medium with or without toxoflavin exposed to wheat fields for 1 h had 84% or 25%, respectively, of colonies identified as F. graminearum. This selection medium provides an efficient tool for isolating F. graminearum, and can be adopted by research groups working on genetics and disease forecasting.

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References

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