• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권11호
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• pp.5354-5369
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• 2019

Sensor networks are deployed in unheeded environment to monitor the situation. In view of the unheeded environment and by the nature of their communication channel sensor nodes are vulnerable to various attacks most commonly malicious packet dropping attacks namely blackhole, grayhole attack and sinkhole attack. In each of these attacks, the attackers capture the sensor nodes to inject fake details, to deceive other sensor nodes and to interrupt the network traffic by packet dropping. In all such attacks, the compromised node advertises itself with fake routing facts to draw its neighbor traffic and to plunge the data packets. False routing advertisement play vital role in deceiving genuine node in network. In this paper, behavior based routing misbehavior detection (BRMD) is designed in wireless sensor networks to detect false advertiser node in the network. Herein the sensor nodes are monitored by its neighbor. The node which attracts more neighbor traffic by fake routing advertisement and involves the malicious activities such as packet dropping, selective packet dropping and tampering data are detected by its various behaviors and isolated from the network. To estimate the effectiveness of the proposed technique, Network Simulator 2.34 is used. In addition packet delivery ratio, throughput and end-to-end delay of BRMD are compared with other existing routing protocols and as a consequence it is shown that BRMD performs better. The outcome also demonstrates that BRMD yields lesser false positive (less than 6%) and false negative (less than 4%) encountered in various attack detection.

• 센서학회지
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• 제26권6호
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• pp.420-426
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• 2017

A colorimetric sensor was investigated to achieve a low-cost warning device for harmful gaseous formaldehyde (HCHO). The sensor is based on selective reactions between hydroxylamine sulfate and HCHO, leading to the production of sulfuric acid. The produced acid results in color-changing response through the acid-base reaction with dye molecules impregnated on a solid membrane substrate. For attaining this purpose, sensors were fabricated by drop-casting a dye solution prepared using different pH indicators on various commercially available polymer sheets, and their colorimetric responses were evaluated in terms of sensitivity and reliability. The colorimetric sensor using bromophenol blue (BPB) and nylon sheet was found to exhibit the best performance in HCHO detection. An initial bluish green of a sensor was changed to yellow when exposed to gaseous formaldehyde. The color change was recorded using an office scanner and further analyzed in term of RGB distance for quantifying sensor's response at different HCHO(g) concentrations. It exhibited a recognizable colorimetric response even at 50 ppb, being lower than WHO's standard of 80 ppb. In addition, the sensor was found to have quite good selectivity in HCHO detection under the presence of common volatile organic compounds such as ethanol, toluene, and hexane.

• Bulletin of the Korean Chemical Society
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• 제35권4호
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• pp.1159-1164
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• 2014

A simple, highly sensitive and selective method based on the rhodamine B-covered gold nanoparticle with dual-readout (colorimetric and fluorometric) detection for $\small{L}$-cysteine is proposed. A mechanism is that citrate-stabilized AuNPs were modified with RB by electrostatic interaction, which enables the nanometal surface energy transfer (NSET) from the RB to the AuNPs, quenching the fluorescence. In the presence of $\small{L}$-cysteine, it was used as a competitor in the NSET by the strongly Au-S bonding to release RB from the Au surface and recover the fluorescence, and the red-to-purple color change quickly, which was monitored simply by the naked eye. Under the optimum conditions, the detection limit is as low as 10 nM. The method possessed the advantages of simplicity, rapidity and sensitivity at the same time. The method was also successfully applied to the determination of $\small{L}$-cysteine in human urine samples, and the results were satisfying.

• 한국정보통신학회논문지
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• 제13권3호
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• pp.564-571
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• 2009

본 논문에서는 다중 주파수 채널이 운용중인 CR 기지국에서 외부대역 협력 스펙트럼 센싱 방식을 제안하였다. 그리고 ATSC 디지털 TV 신호인 경우에 대한 신호검출결과를 제시하였고 주파수 선택적 Rayleigh 페이딩 채널에서 외부대역 협력 센싱 방법에 대한 신호검출확률과 오경보 확률을 유도하였다. 수치해석 결과로는 CR 기지국에서 통신 중인 다중 주파수 채널의 CR단말기들이 측정한 incumbent 신호의 채널 전력이 유사한 경우에는 외부대역 협력 스펙트럼 센싱을 시행함으로써 incumbent 신호의 센싱 성능이 향상됨을 보여주고 있다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2002년도 추계학술대회 논문집 전기물성,응용부문
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• pp.60-63
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• 2002

This paper presents bead-based microbiocihps to detect and separate target proteins. Micro beads coated with capture proteins were introduced into a microchamber, and target proteins flowing across the chamber were bound and concentrated. The chip was connected with an external fluid system. Bead surfaces were double-coated with photo-cleavable linkers and capture proteins. The proteins bound on the beads were photo-separated under UV irradiation, and excited to be measured in fluorescence. $38{\sim}50{\mu}m$ sized polystyrene beads were used. SOGs(silicon-on-glass) were used to fabricate the microchip having glasses bonded on both sides. 100 ${\mu}m$ thick silicon channel was formed through silicon deep RIE process. The upper glass cover had holed through to have inlets and outlets fabricated by powder-blastings. In this study, biotin and streptavidin were used as capture proteins and detection proteins, respectively. The protein mixtures of streptavidin, HSA(human serum albumin) and ovalbumin were applied for selective detection test.

• Journal of Dairy Science and Biotechnology
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• 제39권2호
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• pp.51-62
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• 2021

Escherichia coli are the predominant facultative bacteria found in the gastrointestinal tract of animals and humans. Some strains of E. coli that acquire virulence factors and cause foodborne and waterborne diseases in humans are called pathogenic E. coli and can be divided into five pathotypes according to the virulence mechanism: EAEC, EHEC, EIEC, EPEC, and ETEC. Although selective media have been developed to detect E. coli, distinguishing pathogenic strains from non-pathogenic ones is difficult because of their similar biochemical properties. Therefore, it is very important to find a new and effective diagnostic method to identify pathogenic E. coli. With recent advances in molecular biology and whole genome sequencing, the use of polymerase chain reaction (PCR) is increasing rapidly. In this review paper, we provide an overview of pathogenic E. coli and present a review on PCR detection methods that can be used to diagnose pathogenic E. coli. In addition, the possibility of real-time PCR incorporating IAC is introduced. Consequently, this review paper will contribute to solving the current challenges related to the detection of pathogenic E. coli.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권6호
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• pp.428-434
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• 2004

Coarse clumping of solid materials within diseased biological cells can have a marked influence on the light scattering pattern. Perturbations in refractive index lead to distinct varia­tions in the cytometric signature, especially apparent over wide scattering angles. The large dynamic range of scattering intensities restricts collection of data to narrow angular intervals be­lieved to have the highest potential for medical diagnosis. We propose the use of an interfer­ence filter to reduce the dynamic range. Selective attenuation of scattering intensity levels is expected to allow simultaneous data collection over a wide angular interval. The calculated angu­lar transmittance of a commercial shortwave-pass filter of cut-off wavelength 580 nm indicates significant attenuation of scattering peaks below ${\~}\;10^{circ}$, and reasonable peak equalization at higher angles. For the three-dimensional calculation of laser light scattered by cells we use a spectral method code that models cells as spatially varying dielectrics, stationary in time. How­ever, we perform preliminary experimental testing with the interference filter on polystyrene microspheres instead of biological cells. A microfluidic toolkit is used for the manipulation of the microspheres. The paper intends to illustrate the principle of a light scattering detection system incorporating an interference filter for selective attenuation of scattering peaks.

• Bulletin of the Korean Chemical Society
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• 제26권11호
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• pp.1738-1742
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• 2005

In this study a novel triiodide ion-selective electrode based on a charge transfer complex of iodine and Bis(2-hydroxyacetophenone)butane-2,3-dihydrazone (ICT), as a membrane carrier was prepared. The electrode has a linear dynamic range between 1.0 ${\times}$ $10^{-2}$ and 5.0 ${\times}$ $10^{-7}$ M, with a Nernstian slope of 58. 99 ${\pm}$ 0.3 mV $decade^{-1}$ and detection limit of 3.0 ${\times}$ $10 ^{-7}$ M. The potentiometric response of the proposed sensor is independent of the pH of the solution in the pH range of 3.0-10.0. The electrode possesses the advantages of short conditioning time, fast response time, and especially, very good selectivity over a large number of common organic and inorganic anions. The electrode can be used for at least 6 months without any considerable divergences in the potentials. It was used as an indicator electrode in potentiometric titration of triiodide ion with thiosulfate.

• Bulletin of the Korean Chemical Society
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• 제29권6호
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• pp.1173-1178
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• 2008

A simple and sensitive high-performance liquid chromatographic method was developed for the simultaneous identification of enrofloxacin and its active metabolite ciprofloxacin in chicken muscle. Norflorxacin imprinted polymers synthesized in water-containing systems show high selectivity to enrofloxacin and ciprofloxacin in an aqueous environment. Using these water-compatible imprinted polymers as selective adsorbents in the solid-phase extraction of enrofloxacin and ciprofloxacin from chicken samples, the remaining biological matrix could be quickly washed out from the imprinted column while enrofloxacin and ciprofloxacin were selectively retained and enriched. Analytical separation was performed on a $C_{18}$ column using acetonitrile-water as a mobile phase and fluorescence detection. Good linearity was obtained from 0.8 to 500 ng/g (r > 0.998) with relative standard deviation of less than 3.9%. The mean recoveries of enrofloxacin and ciprofloxacin from chicken muscle were 80.6-94.5% and 77.8-91.8% at three different concentrations. The limits of determinations based on S/N=3 were 0.07 ng/g and 0.09 ng/g, which are below the maximum residue limits established in many countries.

• 디지털콘텐츠학회 논문지
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• 제19권4호
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• pp.757-770
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• 2018

열화상 영상기반 감시 시스템에서 전경추출은 매우 중요한 단계이다. 전경추출단계는 계산시간과 메모리 사용측면에서 시스템의 실시간 처리가 매우 효율적이어야 한다. 그러나 이러한 효율성은 ROI 탐지의 정확도와 매우 연관되어 있다. 본 논문에서 열화상 비디오 처리를 위하여 새로운 히스토그램 빈에 기반하여 배경과 전경을 분리하기 위한 두 가지 방법을 제시하는데, 이는 임의의 주어진 환경에서 열화상영상의 시간상에서 일관성을 갖는 다는 점과, 이러한 성질이, 간단한 시간축 메디안 필터링에 비하여 80%이상의 메모리를 절감할 수 있다.