• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.346-348
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• 2009

A golden double freesia (Freesia hybrida Hort.) "Golden Amor" was developed from a cross 'Golden Crown' and 'White Wing' followed by seedling and line selections at the National Horticultural Research Institute, Rural Development Administration (RDA) in 2005. Growth and flowering characteristic tests were conducted from 2000 to 2005. "Golden Amor" has large double type flower with bright golden color (RHS 12B). It have multiple florets, good flower shape, upright leaves and stalks. The average yield of "Golden Amor" was 6.0 cuttings per plant in greenhouse carried out from 2003 to 2005. It has good production of cormlets, vigorous growth and resistance to Fusarium and virus. "Golden Amor" would be cultured under greenhouse condition in Korea.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.229-233
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• 2008

A new spary chrysanthemum(Dendranthema grandiflorum) cultivar 'Moulin Rouge' was developed from a cross between 'Delmont' and 'Anjela' by selections of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services(ARES) from 2002 to 2006. Its characteristics were investigated three times from 2004 to 2006 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Moulin Rouge' was October 24th, and year-round production was possible by day length treatment. Its capitulum was 5.3 cm in diameter, and had 22.9 ray florets and 12.2 head per stem in autumn. Its ray floret was bi-color(yellow/orange) with green central zone. 'Moulin Rouge' was about 55 days to flower in spring, and showed the vase life of 21.7 days in autumn. This cultivar was registered for a commercialization in 2007.

• Korean Journal of Breeding Science
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• v.42 no.4
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• pp.365-373
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• 2010

TILLING (Targeting Induced Local Lesions IN Genomes) is broadly regarded as an excellent methodology for reverse genetics applications. Approximately 15,000 $M_3$ TILLING lines have been developed via the application of gamma-ray irradiation to rice seeds (cv. Donganbyeo), followed by subsequent selections. In an effort to evaluate the genetic diversity of the TILLING population, we have employed the AFLP multiple dominant marker technique. A total of 96 (0.64%) TILLING lines as well as Donganbyeo were selected randomly and their genetic diversity was assessed based on AFLP marker polymorphisms using 5 primer combinations. An average of 100.4 loci in a range of 97 to 106 was detected using these primer combinations, yielding a total of 158 (31.4%) polymorphic loci between Donganbyeo and each of the 96 lines. A broad range of similarity from 80% to 96% with an average of 89.4% between Donganbyeo and each of the 96 lines was also observed, reflecting the genetic diversity of the TILLING population. Approximately 28 polymorphic loci have been cloned and their sequences were BLAST-searched against rice whole genome sequences, resulting in 20 matches to each of the gene bodies including exon, intron, 1 kb upstream and 1 kb downstream regions. Six polymorphic loci evidenced changes in the coding regions of genes as compared to the rice pseudomolecules, 4 loci of which exhibited missense mutations and 2 loci of which exhibited silent mutations. Therefore, the results of our study show that the TILLING rice population should prove to be a useful genetic material pool for functional genomics as well as mutation breeding applications.

• Korean Journal of Breeding Science
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• v.40 no.2
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• pp.201-205
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• 2008

'Morningpurple' is a new sweetpotato variety developed by Mokpo Experiment Station, National Institute of Crop Science (NICS), RDA in 2007, for flowering. This variety was selected from the cross between Sinchunmi and Hi-dry in 2004, seedling and line selections were practiced from 2005 to 2006. 'Morningpurple' has morning glory type flowers with purple color. It has triangular leaf, green with purple vine and petiole, elliptic storage root, red skin and yellow flesh color of storage root. The average yield of storage root was 15.0 ton/ha. Number of storage roots over 50 gram per plant was 2.6, and the average weight of storage root was 143 gram.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.430-434
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• 2008

'Morningwhite' is a new sweetpotato variety developed by Mokpo Experiment Station, National Institute of Crop Science (NICS), RDA in 2007, with white flowering. This variety was selected from the cross between "Sinjami" and "Muan 10" in 2004, seedling and line selections were applied in 2005 and 2006. 'Morningwhite' is morning glory type flowers with white color. It has Lobed leaf, green vine and petiole, elliptic storage root, red skin and yellow flesh color of storage root. The average yield of storage root was 14.0 ton/ha. Number of storage roots over 50 gram per plant was 2.5 and the average weight of storage root was 140 gram.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.490-494
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• 2008

A new spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Eye Ball' was developed from a cross between 'Rima' and 'Yen' followed by selections of seedlings and lines at the Flower Rearch Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 2002 to 2006. Its characteristics were investigated three times from 2004 to 2006 under forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Eye Ball' was October 20th, but year-round production was possible by shading and lighting. Its flower was 3.1cm in diameter, and had 38.4 petals and 26.8 flowers per stem in autumn. Its petal was narrow (0.4 cm) and pink with green in color at the flower center. To flower in the short day condition, for 'Eye Ball' was about 47 days in spring, and 'Eye Ball' showed a vase life of 20.9 days in autumn. This cultivar was registered for a commercialization in 2007. 'Eye Ball' would be cultured under the greenhouse and rain shelter condition in Korea.

• Journal of Microbiology and Biotechnology
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• v.29 no.6
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• pp.913-922
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• 2019

Magnetic $Ni_{0.7}Co_{0.3}Fe_2O_4$ nanoparticles that were prepared via the rapid combustion process were functionalized and modified to obtain magnetic $Ni_{0.7}Co_{0.3}Fe_2O_4@SiO_2-CHO$ nanocomposites, on which penicillin G acylase (PGA) was covalently immobilized. Selections of immobilization concentration and time of fixation were explored. Catalytic performance of immobilized PGA was characterized. The free PGA had greatest activity at pH 8.0 and $45^{\circ}C$ while immobilized PGA's activities peaked at pH 7.5 and $45^{\circ}C$. Immobilized PGA had better thermal stability than free PGA at the range of $30-50^{\circ}C$ for different time intervals. The activity of free PGA would be 0 and that of immobilized PGA still retained some activities at $60^{\circ}C$ after 2 h. $V_{max}$ and $K_m$ of immobilized PGA were 1.55 mol/min and 0.15 mol/l, respectively. Free PGA's $V_{max}$ and $K_m$ separately were 0.74 mol/min and 0.028 mol/l. Immobilized PGA displayed more than 50% activity after 10 successive cycles. We concluded that immobilized PGA with magnetic $Ni_{0.7}Co_{0.3}Fe_2O_4@SiO_2-CHO$ nanocomposites could become a novel example for the immobilization of other amidohydrolases.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.12
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• pp.1974-1979
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• 2018

Objective: This study compared fatty acid compositions and antioxidant activities of high-preference cuts (loin, tenderloin, and rib) and low-preference cuts (brisket, topside, and shank) of Hanwoo (Bos taurus coreanae) cows to obtain potentially useful information for promoting the consumption of various low-preference cuts. Methods: Individual 500 g samples of fresh beef were collected from each of the six cuts from 10 Hanwoo cows (quality grade 1) and immediately freeze-dried. The dried samples were evaluated for fatty acid composition, free radical scavenging activities (hydroxyl, alkyl, and 2, 2'-diphenyl-1-picrylhydrazyl [DPPH] radical), and antioxidative enzyme activities (glutathione peroxidase [GPx], glutathione-S-transferase [GST], and superoxide dismutase [SOD]). Results: The percentages of total polyunsaturated fatty acids were significantly higher in low-preference cuts than in high-preference cuts (p<0.05). Hydroxyl, alkyl, and DPPH radical scavenging activities were significantly higher in low-preference cuts than in high-preference cuts (p<0.05). In addition, the activities of antioxidant enzymes, such as GPx, GST, and SOD, were significantly higher in low-preference cuts compared with high-preference cuts (p<0.05). Conclusion: These results may influence consumers to include more low-preference cuts in their selections based on the nutritional facts, which could help to balance the beef market in South Korea.

• Journal of Sasang Constitutional Medicine
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• v.31 no.2
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• pp.1-11
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• 2019

Background Sasang constitutional medicine explains that the difference in eating habits depending on constitutional characteristics affects health status. The aim of the present study is to investigate research trend on Sasang constitution (SC) and eating habits. Method Research papers from 2010 to 2019 on SC and eating habits were searched in domestic and international 5 databases. As a result, 11 clinical studies related to the SC and eating habits were finally selected. From the finally selected research papers, subjects, study period, constitutional diagnostic tools used for studies, and eating habit evaluation tools were investigated. Results In a total of 11 research papers conducted as clinical studies regarding SC and eating habits for the recent 20 years, eating speed and meal size items were most frequently used, followed by the regularity of meals, unbalanced diets, breakfast. Taeumin was more likely to eat fast, eat a lot of food, or overeat while Soeumin was less likely to overeat or they eat less food. There was no significant difference in the regularity of meals depending on constitution, but each study showed different tendency. Conclusion This study closely reviewed Sasang constitutional eating habit items from the precedent studies to select the major items of eating habits as a constitutional health management index. The results of this study will contribute to selections of items and the development of tools to measure constitutional eating habits related to health status.

• The Journal of Internal Korean Medicine
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• v.40 no.4
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• pp.649-662
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• 2019

Objective: The purpose of this study was to investigate the effect of electroacupuncture on acute pancreatitis (AP). Method: The search engines that were used included PubMed, CNKI, OASIS, NDSL, the Korean Traditional Knowledge Portal, and J-STAGE. The search period was from the creation of the search engine to May 6, 2019; there were no language limitations. Extractions and selections from the literation were made by two authors. The study included randomized controlled trials with electroacupuncture for patients with acute pancreatitis. Cochrane's risk of bias (RoB) was used as the methodological quality assessment scale. Results: Nine studies were finally selected. It was observed that using electroacupuncture with general Western medicine treatment for AP was more effective than using only general Western medicine treatment. Although most of the studies showed a high risk of bias, there were significant differences among the indicators. Conclusion: The electroacupuncture treatment with Western medicine may be more effective in reducing symptoms of AP than Western medicine alone. However, due to the limitations of the research design, it was not enough to obtain reliable information. As a result, high quality research will be additionally required.