• Title/Summary/Keyword: Selection of Variety

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A New Variety "Singang" with resistance to Soybean Mosaic Virus by Molecular Marker Assisted Selection (분자표지선발(MAS)을 이용한 콩모자이크병 저항성 신품종 "신강")

  • Lee, Seuk-ki;Moon, Jung-Kyung;Jeong, Kwang-Ho;Seo, Min-Jung;Kim, Yul-Ho;Yun, Hong-Tae;Kim, Jeong-Kon
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.568-573
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    • 2009
  • A new soybean variety, "Singang", was released by NICS, RDA in 2008. It is resistant to soybean mosic virus (SMV), which is one of the most serious diseases in soybean production. "Singang" had been developed for seven years by molecular marker-assisted selection (MAS) breeding method using recurrent parent, "Sowon", and donor parent, "L29" which has the SMV-resistance gene, Rsv3. Selected promising breeding line, Suwon 242, was evaluated in yield trials for 2 years from 2005 to 2006 at NICS. Regional yield trials were performed for 3 years from 2006 to 2008 in five locations including Jeju. Agronomic characteristics of "Singang" is a determinate growth habit, purple flower color, grey pubescence, yellow testa, light brown hilum and lanceolate leaflet shape. "Singang" showed the similar performance to "Sowon" cultivar. This variety showed 3 days shorter in days to maturity than the check variety (Pungsannamul). The average yield under regional yield trials in five location was 2.78t/ha. It is adaptable to the whole country except for Gangwon province.

Identification of SNPs tightly linked to the QTL for pod shattering in soybean[Glycine max (L.) Merr.]

  • Kim, Kyung-Ryun;Kim, Kyung Hye;Go, Hong Min;Lee, Ju Seok;Moon, Jung-Kyung;Ha, Bo-Keun;Jeong, Soon-Chun;Kim, Namshin;Kang, Sungtaeg
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.146-146
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    • 2017
  • The pod shattering or dehiscence is essential for the propagation of pod-bearing plant species in the wild, but it causes significant yield losses during harvest of domesticated crop plants. Identifying novel molecular makers, which are linked to seed-shattering genes, is needed to employ the molecular marker-assisted selection for efficiently developing shattering-resistant soybean varieties. In this study, a genetic linkage map was constructed using 115 recombinant inbred lines (RILs) developed from crosses between the pod shattering susceptible variety, Keunol, and resistant variety, Sinpaldal. A 180 K Axiom(R) SoyaSNPs data and pod shattering data from two environments in 2001 and 2015 were used to identify quantitative trait loci (QTL) for pod shattering. A major QTL was identified between two flanking single nucleotide polymorphism (SNP) markers, AX-90320801 and AX-90306327 on chromosome 16 with 1.3 cM interval, 857 kb of physical range. In sequence, genotype distribution analysis was conducted using extreme phenotype RILs. This could narrow down the QTL down to 153 kb on the physical map and was designated as qPDH1-KS with 6 annotated gene models. All exons within qPDH1-KS were sequenced and the 6 polymorphic SNPs affecting the amino acid sequence were identified. To develop universally available molecular markers, 38 Korean soybean cultivars were investigated by the association study using the 6 identified SNPs. Only two SNPswere strongly associated with the pod shattering. These two identified SNPs will help to identify the pod shattering responsible gene and to develop pod shattering-resistant soybean plants using marker-assisted selection.

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Radio-sensitivity Analysis and Selection of Useful Mutants of Rape (Brassica napus L.) by Gamma Irradiation (방사선 처리에 의한 유채의 생육 및 감수성 조사)

  • Goh, Eun Jeong;Kim, Wook-Jin;Kim, Jin-Baek;Kim, Dong Sub;Kim, Sang Hoon;Kang, Si-Yong
    • Journal of Radiation Industry
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    • v.4 no.3
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    • pp.277-283
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    • 2010
  • Rape (Brassica napus L.) plants are one of the major oilseed crops. The main components of rapeseed are oil (35 to 47%) and protein (15 to 32%). For the biodiesel production, the development of a new variety of rape plant with high biomass and/or oleic acid contents is required. In order to determine the optimum dose of gamma-ray irradiation, the rape seeds of cvs. Hanra (Hr), Youngsan (Ys), Tammi (Tm), and Tamra (Tr) were irradiated with a 100~4,000 Gy dose range of gamma-rays. Considering the growth factors, the optimum doses were determined to be within the range of 600~1,000 Gy for the selection of useful mutant lines. Six-hundred and eighty-eight (688) $M_2$ mutant lines were obtained from 600~1,000 Gy gamma-ray-irradiated $M_1$ plants through selfing. The growth characteristics, leaf shape, early flowering, and flower color were all investigated. The selected mutant numbers of early flowering, leaf shape, and flower color were 34, 52, and 3 from the four cultivars, respectively. These mutant lines will be used for the development of a new variety of rape plant with high biomass and oleic acid contents.

Determinant Factors of Rice Farmers' Selection of Adaptation Methods to Climate Change in Eastern Rwanda (동부 르완다 쌀 농업인의 기후변화에 대한 적응 방법 결정 요인)

  • Butera, Tonny;Kim, Tae-Kyun;Choi, Se-Hyun
    • Korean Journal of Organic Agriculture
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    • v.30 no.2
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    • pp.241-253
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    • 2022
  • The negative impact of climate change on the agricultural sector is rapidly increasing, and it is urgent to prepare policies at the government level to mitigate it. In the case of Rwanda's agricultural sector, which lacks the government's budget and farmers' capital, efficient and effective policy implementation is of paramount importance. To this end, rather than establishing related policies in the public sector from the top down, it is necessary to establish a bottom-up customized policy that is reflected in policy establishment by identifying the characteristics and behaviors of farmers who actually participate in adaptation activities. In this study, the effects of farmers' characteristics and farmers' perception status/adaptation status to climate change on the selection of adaptation methods for climate change were analyzed. 357 rice farmers randomly selected from Eastern Rwanda were surveyed to explore the information related to farmers' perception to climate change and adaptation methods as well as basic information of the farm. Research shows that the probability of selecting a variety of adaptation methods rather than not responding to climate change increases the younger the age, the higher the education level, and the easier access to climate information and credit. As a policy proposals, it is judged that public support such as strengthening agricultural technology support services, including more detailed guidance for elderly and low-educated farmers, and improving access to farm loan services by agricultural financial institutions is needed. In addition, it is necessary to adjust the planting time and cultivation method, provide timely information related to climate change, and provide crop variety improvement services to farmers.

Some Facts in the Course of the Segregation and Selection of the Sex-limited Inheritance Character of Silkworm Larval Marking (한성형잠 분리 선발 과정에서의 몇 가지 사실에 관하여)

  • 이상풍;홍기원;김계명
    • Journal of Sericultural and Entomological Science
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    • v.17 no.1
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    • pp.47-53
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    • 1975
  • This experiment was carried out to obtain a marked larvae in sex-limited inheritance, using F$_1$ hybrid Bo ok X Chun san introduced from Japan. Sequence of backcrossing has been done through the earlier generation with a recessive character of plain marked larvae. 1. It is noted that genotypic segregation of sex-limited larval marking was observed in F$_4$ generation; female possesses larval marking and male shows a plain marking. 2. Larval marking heredity follows a diagram of segregation with an expected genotype such as ♀ : (W$.$+p)/Z$.$P/P and ♂ : Z/Z$.$P/P. 3. It is observed that dissociation was occurred to produce female with a genotype of W/Z$.$P/P in segregation ana selection. 4. Abnormal ratio of sexuality is observed in the course of segregation and segregation and analysis of it is continuously under way. 5, It is observed that the difference of qualitative characteristics between female and male obtained from the original variety shows the same tendency as the normal marked variety.

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Breeding of a New Saxifraga fortunei Cutivar (Hybrid) 'Whitegreen' with Light Green and White Colored Petals as a Pot Flower (밝은 연녹색의 분화용 바위떡풀 신품종 '화이트그린' 육성)

  • Suh, Jong-Taek;Hong, Su-Young;Yoo, Dong-Lim
    • Korean Journal of Plant Resources
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    • v.27 no.5
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    • pp.588-592
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    • 2014
  • A new Saxifraga fortunei cultivar 'Whitegreen' was bred by crossing between 'Daewoojoo' with Pale Green (Y-G145-C) colored petals and 'Kwanglim' with light Yellow green (Y-G145A) colored petals. The selection and flower characterization were conducted from 2004 to 2008 in greenhouse of Highland Agriculture Research Center, NICS, RDA. Flowering in 'Whitegreen' Showed from September 20 th for 33 days. The number of petals were 11.4 ea., with a width of 1.4 cm and length of 1.4 cm. The plant height was 20.7 cm, and number of leaves per plant was 48.3 ea., with a width of 3.4 cm and length of 3.0 cm. 'Whitegreen' cultivar can be used as a pot flower. 'Whitegreen' plants grown under 50% shade of sunlight have higher degree of acclimation compared to those under direct sunlight. And soft rot disease would occur usually by over-irrigation. 'Whitegreen' cultivar was registered as a novel variety with No. 3456 in Korea seed and variety service on April 2011.

Graft Selection and Fixation in Anterior Cruciate Ligament Reconstruction (전방십자인대 재건술 시 이식건의 선택과 고정)

  • Kim, Du-Han;Bae, Ki-Cheor;Choi, Byung-Chan
    • Journal of the Korean Orthopaedic Association
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    • v.55 no.4
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    • pp.294-304
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    • 2020
  • Anterior cruciate ligament (ACL) reconstruction is a successful procedure independently by patient selection, timing of surgery, surgical technique, choice of graft, and fixation methods. Among these factors, graft selection and fixation methods might be the most critical yet controversial questions for surgeons. Although recent studies showed that grafts have advantages and drawbacks, there is still no ideal graft. Similarly, many fixation methods of femoral and tibial tunnels have been proposed over the last few decades, with no clear superiority of one technique over another. Surgeons should be familiar with a variety of grafts, fixation techniques, and their specific associated surgical procedures as well as the advantages and disadvantages of each. Therefore, this article summarizes the current literature and discusses the current state of graft selection and fixation methods in the treatment of an ACL injury.

Permanent Mycoplasma Removal Removel from Tissue Culture Cells: A Genetic Approach

  • Motr, Gabriele;Preininger, Alexandra;Himmelspach, Michele;Plaimauer, Barbara;Arbesser, Christine;York, Heinz;Dorner, Friedrich;Schlokat, Use
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.2
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    • pp.84-91
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    • 2000
  • Mycopasma contamination of tissue culture cells easily evades detection and, thus, represents a continous therat to cell biologists. In case where infected cell can not simply be replaced, attempts have to be made to eradicate mycoplacma from the tissue culture cells. A variety of anti-microbial agents have been shown to be toxic to mycoplasma strains ; however, cell associated mycoplasma are often protected from antibiotics at concentrations shown to be effective in vitro. Antibiotic concentrations high enough to be lethal to cell as sociated mycoplasmas frequently are also detrimentrations to the host cells, while moderately increased antibiotic levels tolerated by the host cells often lead to only temporary growth suppression and/or to the emergence of mycoplasma strains resistanct even to high concentrations of the antibiotis applied. Hare, a genetic approach for the elimination of mycoplasma from tissue culture cells that overcomes thens limitations is described. By expression of a selection marker conferring resistance to an otherwise toxic agent, Acholeplasma laidlawii infected BHK-21 cells used as the model system were enabled to temporarily tolerate antibiotic concentrations high enough to be lethal to cell associated mycopalsma while leaving the host cells unharmed. Upon successful mycoplasma eradicated, cultvation of the cured host cells in the absence of the selective agent yielded revertant cell clones that had regained susceptibillity to the toxic agent. Cressation of the selection marker expression was shown to result from the loss of the selection marker DNA, which is a consequence of the fact that the stable and permanent integration of foreign DNA in eucaryotic cell chrosomes is highly inefficient. Thus, the cells were cured from mycoplasma yet remained biochemically unaltered.

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A Study on Competitiveness Improvement Strategies of Korean Coffee Franchisers in Beijing, China through a Positioning Analysis (포지셔닝 분석을 통한 국내 커피전문기업의 중국 베이징 지역 내 경쟁력 강화방안 연구)

  • Guo, Ying-Chao;Kim, Hyun-Jung
    • Culinary science and hospitality research
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    • v.22 no.5
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    • pp.37-51
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    • 2016
  • The purposes of this study were (1) to investigate the important selection attributes of coffee brands and the relative positions of major coffee brands on the positioning map through similarities and preferences of coffee brands that customers perceive and (2) to provide Korean coffee brands with strategic information about how to differentiate themselves from existing competitors in the Chinese market. A total of 268 samples were used for data analysis that comprised frequency analysis, descriptive statistics, ALSCAL, and regression analysis. The results showed that among 8 coffee brand selection attributes the most important was atmosphere, followed by cleanness and sanitary condition, and convenient location and transportation. Friendly service, taste of coffee, price and variety of menu were next and brand was the least important among 8 selection attributes. The results of positioning map showed that Maan Coffee was evaluated the highest iin atmosphere, cleanness and sanitary condition, and convenient location and transportation compared to other coffee brands. Marketing strategies for Korean coffee franchisers (Angel-in-us Coffee and Caffe Bene) were provided for successful repositioning in the Beijing coffee market.

SHADE ANALYSIS OF ANTERIOR TEETH USING DIGITAL SHADE ANALYSIS SYSTEM (Digital Shade Analysis System을 이용한 전치부의 색조 특성에 관한 연구)

  • Kim Hee-Eun;Cho In-Ho;Lim Ju-Hwan;Lim Hun-Song
    • The Journal of Korean Academy of Prosthodontics
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    • v.41 no.5
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    • pp.565-581
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    • 2003
  • Statement of problem : A scientific examination and understanding of overall aspects of the natural dentition is the first step involved in making a satisfactory selection in the shade of an aesthetic prosthesis. Proper natural shade selection of the prosthetic restoration that is in harmony with the remaining dentition is as important aesthetically, as harmony of form and function in the anterior dentition. Clinically, the most commonly applied method of shade selection has been visual, but because of the subjective nature inherent to this method, shade selection results are variable and can be influenced by such factors as the technician, the type of shade guide used, and the type and intensity of the lighting. Purpose : The purpose of this study was to develop a more objective and scientific approach to examining and understanding the shade of teeth, which has in turn lead to the development of a number of shade analysis devices that present a more objective method of shade analysis. Material and Method : In this study, the shades of healthy anterior teeth were examined and analyzed using the recently developed digital shade analysis of the $ShadeScan^{TM}$ System. The study examined 80 individuals in their twenties, 40 males and 40 females, presenting 6 healthy, unrestored maxillary anterior teeth. Tooth brushing and oral prophylaxis were performed prior to evaluation. The ShadeScan handpiece was used to acquire images of the 6 maxillary anterior teeth. These images were analyzed using the Vita/Classical mode of the $ShadeScan^{TM}$ Software, and shade maps of each tooth were acquired and divided into cervical, middle, and incisal thirds. The shade distribution of each third, left and right symmetry, and gender differences were investigated and analyzed. Results : The results of the study are as follows : 1. An overwhelming majority of the examined teeth were found to possess shades belong to Group A, with the greatest variations occurring at the middle and cervical thirds of the maxillary central and lateral incisors, in both male and female subjects. 2. Canines of both male and female subjects showed left and right symmetry with uniform shade distribution of A4 and C4, while the lateral and central incisors showed left-right symmetry of the incisal 1/3 with a uniform shade distribution of A2 and A3 shades 3. No significant differences in shade distribution were seen between genders in maxillary canines, whereas maxillary central and lateral incisors showed differences at the middle and cervical thirds between male and female subjects The results of this study show that with the exception of maxillary canines, maxillary anterior teeth display a diverse shade distribution as well as gender differences. Conclusion : Clinically, when making a shade selection using the existing shade guide, one must consider the fact that even a single tooth consists of a variety of shades. The results of this study show that when selecting a shade from a number of groups is difficult, shades from A group are the most consistent with the natural shade or maxillary anterior teeth.