• BMB Reports
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• v.37 no.3
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• pp.376-382
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• 2004

The Epstein-Barr-transformed B lymphoblastoid cell lines, LCL, which express antigens, are potential antigen-presenting cells (APCs) for the induction of cytotoxic T lymphocytes in vitro. However, transfecting LCL with subsequent selection by antibiotics is notoriously difficult because the plating efficiencies of LCL are reported to be 1% or less. Therefore, this study investigated the optimal conditions for increasing the transduction efficiency of a recombinant adenovirus to LCL for use as a source of APCs. The transduction efficiencies were < 13% (SD $\pm$ 2.13) at a multiplicity of infection (MOI) of 100, while it was increased to 28% (SD $\pm$ 9.43) at an MOI of 1000. Moreover, its efficiencies to LCL that expressed the coxsackie adenovirus receptor were increased to 60% (SD $\pm$ 6.35) at an MOI of 1000, and were further increased to 70% (SD $\pm$ 4.56) when combined with the centrifugal method. The cationic liposome or anionic polymer had no effect on the transduction efficiency when compared to that of the centrifugal method. These results may be used as a convenient source of target cells for a CTL assay and/or autologous APCs for the induction of the in vitro CTL responses that are specific to viral and tumor antigens.

• Journal of Nutrition and Health
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• v.53 no.4
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• pp.431-444
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• 2020

Purpose: The purposes of this study were to understand the health interest of consumers in the United States and Japan and thus understand the motivation of food choices, in order to provide basic data on the country's strategy based on healthy and correct food choices in the future. Methods: A customer survey was conducted in 2019 from October 18 to 31, and it targeted 115 American and 120 Japanese local consumers between the ages of 20- to 64-years-old. Eight questions were formed using General Health Interest. Based on food choice motivation, 27 questions were reconstructed and asked about demographic information. All data were analyzed by SPSS Statistics (ver. 25). Results: Health consciousness was categorized into 2 types: nutrition-seeking type and preference-seeking type. Based on these 2 factors, customers were grouped into 3 clusters: healthy dietary life-seeking group, nutrition balance-seeking group and health indifference group. Food choice motivation was categorized into 4 types: health, efficiency, value, and mood. All 3 groups showed a high tendency for efficiency in common. The results show that consumers want higher satisfaction with their time and money invested in food consumption. It is believed that the focus and investment of market segmentation strategy should be focused on product development, especially for American and Japanese consumers who are interested in health. Conclusion: The results of this study reflect consumer needs that can assist in the selection of healthy and correct foods in the future.

• Journal of Wetlands Research
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• v.18 no.2
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• pp.194-200
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• 2016

The impervious surface rate increased by urbanization causes various problems on the environment such as water cycle distortion, heat island effect, and non-point pollutant discharges. The Low Impact Development (LID) techniques are significantly considered as an important tool for stormwater management in urban areas and development projects. The main mechanisms of LID technologies are hydrological and environmental pollution reduction among soils, media, microorganisms, and plants. Especially, the media provides important functions on permeability and retention rate of stormwater runoff in LID facilities. Therefore, this research was performed to assess the pollutant removal efficiency for different types of media such as zeolite, wood chip, bottom ash, and bio-ceramic. All media show high pollutant removal efficiency of more than 60% for particulate materials and heavy metals. Double layered media is more effective in reducing heavy metals by providing diverse sizes of micro-pores and macro-pores compared to the single layered media. The results recommend the use of different sizes of media application is more cost-effective in LID than a single size of media. Furthermore, soluble proportion of total heavy metal in the stormwater is an important component in proper media selection and arrangement.

• Journal of Broadcast Engineering
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• v.14 no.1
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• pp.59-69
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• 2009

A bi-directional symmetric prediction technique has been developed to improve coding efficiency of B-slice and to reduce the computational complexity required to estimate two motion vectors. On the contrary to the conventional bi-directional mode which encodes both forward and backward motion vectors, it only encodes a single forward motion vector, and the missing backward motion vector is derived in a symmetric way from the forward motion vector using temporal distance between forward/backward reference frames to and from the current B picture. Since the backward motion vector is derived from the forward motion vector, it can halve the computational complexity for motion estimation, and also reduces motion vector data to encode. This technique always derives the backward motion vector from the forward motion vector, however, there are cases when the forward motion vector is better to be derived from the backward motion vector especially in scene changes. In this paper, we generalize the idea of the symmetric coding with forward motion vector coding, and propose a new symmetric coding with backward motion vector coding and adaptive selection between the conventional symmetric mode and the proposed symmetric mode based on rate-distortion optimization.

• Journal of the Korean Solar Energy Society
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• v.34 no.2
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• pp.32-43
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• 2014

This study set out to identify the importance of each factor influencing facility selection with a survey among public medical facilities under the category of public buildings and apply the importance of economy, technology and environment with the weighting factor method, thus proposing optimal application plans. The research content of each section can be summarized as follows:1) Estimated energy consumption according to the energy simulation was 65,129MWh/yr, which was 18.7% higher than that according to the calculation equation. Of the energy consumption, more than 80% was used by heating and cooling facilities and construction facilities, and 20% was used by electronics such as medical equipments and in and outdoor lighting. 2) The results of a survey on the factors influencing the importance when selecting a new and renewable energy system reveal that the upper items had a priority in economy, environment, and technology in the descending order and that the lower item shad a priority in initial investments, maintenance and repair costs=energy costs, supply reliability, energy efficiency and $CO_2$ emissions in the descending order. 3) The application alternatives were analyzed in economy, technology, and environment. As a result, a geothermal system turned out to be the most excellent one a cross all the upper and lower comparison items. Of the other systems, a solar thermal system was superior in initial investments, maintenance and repair costs, and energy efficiency, where as a photovoltaic system was superior in energy costs, supply reliability, and $CO_2$ emissions. 4) As for the mixed application ratio among economy, technology, and environment, when the percentage of a geothermal system was approximately 80% or higher in anew and renewable energy system, it was the best and most optimal application plan.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.5
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• pp.666-671
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• 2017

Objective: Aim of the current comparative study was to evaluate production outputs, reproduction efficiency and functional traits in dual-purpose Fleckvieh and Braunvieh cows, reared under temperate European conditions. Methods: A data-set from 414 Fleckvieh and 42 Braunvieh cows and 799 lactations was analysed. ID tag number, milk yield per milking session, number of steps/interval and milk conductivity were recorded and collected daily using AfiMilk 3.076 A-DU software (Afimilk Ltd., Kibbutz, Israel). Production and milk quality data were taken from the results of the official performance recordings and the reproductive outputs of cows were recorded by the research stations veterinarians. Comparisons between the two genotypes were carried out using the one way analysis of variance protocol, with categorical factor being considered the breed of cows. All the statistical inferences were carried out using Statistica software (StatSoft Inc., Tulsa, OK, USA). Results: Fleckvieh cows significantly outperformed ($p{\leq}0.05$) the Braunvieh herd, with average milk yields of $5,252.1{\pm}35.79kg$ and $4,897.6{\pm}128.94kg$, respectively. Age at first calving was significantly ($p{\leq}0.01$) influenced by the breed, with Fleckvieh heifers being more precocious ($32.8{\pm}0.29mo$) compared to those of Braunvieh breed ($35.7{\pm}0.84mo$). Reproduction efficiency as defined by the number of inseminations per gestation, calving interval, dystocia, days dry and days open, was not influenced by genotype (p>0.05). Incidences of sub-clinical mastitis, clinical mastitis, lameness and abortions were not influenced by the breed factor (p>0.05). Stay-ability of cows was significantly ($p{\leq}0.001$) influenced by genotype, with Braunvieh cows having an average age at culling of $117.88{\pm}11.78$ months compared to $90.88{\pm}2.89$ months in Fleckvieh. Conclusion: Overall, results have shown that genotype significantly influenced milk yield, age at first calving and longevity.

• Korean Journal of Construction Engineering and Management
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• v.16 no.3
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• pp.91-100
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• 2015

An increase in energy such as natural gas, coal, oil, has occurred to a large amounts of environment impact emissions, it is necessary to reduce in the construction industry for the energy consumption. To encourage remodeling project in developed countries of the majority, on the basis of this, remodeling project in the construction industry has grown to a large amount. Results of analysis of the research related to the advanced remodeling, analysis of the economic validity in accordance with the production and process and building elapsed years of selection alternative of remodeling there has been a problem that has not been properly reflected. In this study, a decision support model that can simultaneously choose the most cost-effective and energy-efficiency alternative. Developed process model, generates a "Remodeling Solution" that combines the renewable energy equipment and envelope system, energy performance evaluation of the application of international standards(ISO-13790, DIN V 18599), perform the economic evaluation through LCCA(Life Cycle Cost Analysis) technique, circulated evaluation and configured to output the optimal Remodeling Solution. The results of applying the model developed in the case, it was confirmed that it is possible to select a choice of cost-effective energy-saving alternative. Then, developed model through this study, it is expected to be able to help highly effective remodeling alternative to selecting by decision-makers.

• Journal of the Korea Organic Resources Recycling Association
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• v.25 no.2
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• pp.15-26
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• 2017

This study was conducted to investigate and analyze the discharge characteristics of recyclable material from S-city, S-district, in order to improve the sorting efficiency in recycling sorting facility. The characteristics of recyclable materials collected were analyzed in three different scopes; source origin, collection and transportation, and sorting steps. The average of recyclable waste generation is $0.121kg/day^*man$. Regional collection period appears to be three times a week, and the density of mixed recyclable wastes showed the average of $202.4kg/m^3$ in the waste collection vehicle. In the analysis into the sorting steps, the average of carrying amount of mixed recycling products is 1,154.6 ton/month, the average of appeared density is $181kg/m^3$, the average amount of separated recycling products is 448.5kg/month, and the density of recycling residue is found out to be $48kg/m^3$. The sorting rate of recyclable material is 38.85% and the percentage of residues is 55.90%. Out of 7,744.8 tons of the total recyclable residues, 4,272.1 tons were found out to be possible recylable materials. As a result of increasing the recycling rate of residues, the encouragement of base-recycling, the automation and retrofit of sorting equipment, and energy recovery from recycling residue were discussed.

• Journal of Energy Engineering
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• v.25 no.3
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• pp.41-50
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• 2016

This study examined the combustion characteristics and emissions, fuel economy, acceleration by selecting the two fuel with octane number difference to investigate the effect on the combustion characteristics and performance of the vehicle according to the octane number. First, a single-cylinder engine was used for the combustion characteristic experiment, Of the fuel, which is distributed on the market by the selection of two different octane fuel it is performed experiments. Single cylinder experiment examined the combustion characteristics that appear when you gradually advancing the ignition timing by the ignition timing and air-fuel ratio control for each fuel and through an output, emissions, pressure, hence examined the correlation between by octane number. In addition through the actual vehicle compared the changes in the fuel octane number difference, through acceleration tests examined the impact of the octane number requirements for high-performance segment. As a result, fuel of high octane number in accordance with the ignition timing the advancing showed a slightly stable combustion characteristics, a slight increase occurred in the acceleration test and power. However, both fuel does not significantly differ from the current mode, simulating the urban and highway fuel efficiency. Therefore, the operating conditions of the vehicle currently being sold on the Effects of high-octane fuel. fuel efficiency was found insufficient.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.395-400
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• 2017

Atopic dermatitis is a chronic inflammatory skin disease caused by a variety of genetic and environmental factors, dysregulation of immunological response, as well as dysfunction of the skin barrier proteins. The purpose of this study is to develop an ELISA kit suitable for evaluating the expression of skin barrier proteins. Proteins were obtained from the skin via AriNo and D-Squame patches. The efficiency of protein collection from the skin, using the Arino patch, was shown to be more effective than using D-Squame; while the efficiency of lysis using 0.1% Triton-X100 was higher than that of other lysis solutions, including 0.1 M Tris-HCL, 0.1% Tween-20, and 5 mM KOH. Recombinant skin barrier proteins, such as filaggrin and involucrin, were produced by molecular biological methods. Monoclonal antibodies against filaggrin and involucrin were produced by immunization of mice, fusion of spleen cells and myeloma cells, as well as a selection of antibody-producing hybridoma cells. The filaggrin expression in the skin of subjects suffering from atopic dermatitis was lower than that in normal mice. Involucrin expression was not altered between normal individuals and subjects with atopic dermatitis. These findings contribute to an elucidation of the importance of the skin barrier protein expression in atopic dermatitis and the development of a diagnostic kit for atopic dermatitis.