As seed production program is growing prosperously in various fishes in southern Korea, disease problems in larval and juvenile stages have emerged as a new research object. The following results were obtained from investigation about environmental factors related to mass mortalities of young Kinki red seabream, Pagrus major in process of artificial seedling production. Total length of red seabream larvae hatched was 2.93mm, and became 18.83~20.12mm at day 40. The first noticeable mortality of red seabream larvae (7.98~9.37mm) occurred in 25~30 day-old fish with the survival rates of 59.8~60.3%. Thereafter the mortality of larvae decreased, survival rate was 20.5~25.45% in day 40. After 20~30 days, the quality of pond water was bellow II class. During the experimental period COD, $PO_4$-P, $NO_2$-N, $NO_3$-N and $NH_$-N increased up to 3, 7, 34, 6 and 8 times, respectively, compared to initial ones. The number of viable bacteria in pond water and seabream larvae were $6.3{\times}10^6$~$2.3{\times}10^7$ cfu/ml, 4.3~$7.4{\times}10^6$ cfu/g in day 25, respectively. Among the isolated bacteria from the diseased red seabream in day 25, Vibrio spp. was considered to be the causative organism. External symptoms of this disease were floated, spined near the surface and inflated abdomen. When the isolated strain of the Vibrio was bathed to seabream larvae, $LD_50$ of seabream larvae was over $10^6$ cfu/ml of Vibrio spp.
Shin Yong Seub;Kim Jwoo Hwan;Park So Deuk;Park Jong Wook;Kang Chan Koo;Kim Byung Soo;Khan Z.
Journal of Bio-Environment Control
/
v.14
no.1
/
pp.46-51
/
2005
This experiment was conducted to investigate planting depth, which applied the basic data for planting growth ability and mechanical planting of oriental melon. 'Gumssaragi-eunchun' variety was approach grafting to 'Shintozoa' and seedling was growing during 45 days in the 9cm pot and then planted on Jan. 16. The comparison of planting plots was carried out for four experimental plots, which were separated into 0cm, 4.5cm, 9cm, standard cultivation, and 12cm planting depth in soil. In the tunnel of vinyl house, the lowest and the highest temperature was $9.3^{\circ}C\;and\;41.2^{\circ}C$, respectively, and humidity was $59\~99\%$ during Jan. 18 and 19. The faster graft-take rate after planting was the deeper planting depth. The growth of 40 days after planting was not significant in each planting plot, except 0cm experimental plot. Fruit weight was the heaviest in 4.5cm planting depth of experimental plot but sugar content and flesh thickness were not significant in each planting depth. The more depth in planting meant the more increase in fermented fruit rate and decrease in marketable fruit rate. Marketable yield was 2,361kg per l0a in 9cm planting plot, which was $2\%\;and\;1\%$ lower than in 0cm and 4.5cm, respectively, and $11\%$ greater than in 12cm planting plot.
Hwang, Sung Min;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Jin-Cheol;Choi, Gyung Ja
Research in Plant Disease
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v.20
no.2
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pp.119-125
/
2014
Root-knot nematodes represent a significant problem in cucumber, causing reduction in yield and quality. To develop screening methods for resistance of cucumber to root-knot nematode Meloidogyne incognita, development of root-knot nematode of four cucumber cultivars ('Dragonsamchuk', 'Asiastrike', 'Nebakja' and 'Hanelbakdadaki') according to several conditions such as inoculum concentration, plant growth stage and transplanting period was investigated by the number of galls and egg masses produced in each seedling 45 days after inoculation. There was no difference in galls and egg masses according to the tested condition except for inoculum concentration. Reproduction of the nematode on all the tested cultivars according to inoculum concentration increased in a dose-dependent manner. On the basis of the result, the optimum conditions for root-knot development on the cultivars is to transplant period of 1 week, inoculum concentration of 5,000 eggs/plant and plant growth stage of 3-week-old in a greenhouse ($25{\pm}5^{\circ}C$). In addition, under optimum conditions, resistance of 45 commercial cucumber cultivars was evaluated. One rootstock cultivar, Union was moderately resistant to the root-knot nematode. However, no significant difference was in the resistance of the others cultivar. According to the result, we suggest an efficient screening method for new resistant cucumber to the root-knot nematode, M. incognita.
Two ectomycorrhizal fungi, Pisolithus tinctorius and Thelephora terrestris, were introduced form U.S.A. and inoculated to five pine species in Korea to evaluate the reported growth stimulation of host plants after inoculation. These fungi were grown as mycelial inoculum in large quantity and ioculated to the fumigated nursery soil just before seed sowing. At the end of the first growing season. Pisolithus stimulated the height growth of Pinus densiflora. P. thunbergii. P. rigida, and P. rigida x teada by55, 36, 69, and 37%, respectively, compared with control seedlings with no fumigation and no inoculation. When the growth stimulation was expressed with dry weight, Pisolithus increased dry weight of P. densiflora and P. rigida x taeda by 143% and 128%, respectively, over control seedling. Thelephora failed to stimulate growth of inoculated plants. Pinus koraiensis did not respond to the inoculation during the first growing season. It is concluded that artificial inoculation of nursery pine trees with selected mycorrhizal fungi should be seriously considered to improve the quality of planting stocks and to stimulate early plant growth. The potential for use of Pisolithus in reforestation on adverse sited is also discussed.
Park, Hyung Soo;Choi, Ki Choon;Kim, Ji Hea;Lee, Sang-Hoon;Jung, Jong Seong
Journal of The Korean Society of Grassland and Forage Science
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v.35
no.1
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pp.36-42
/
2015
This study was conducted from 2013 to 2014 to explore the feasibility of the cultivation of warm-season grass as an annual Korean forage crop, while concurrently evaluating the characteristics and forage production of warm-season grass in Korea. The experimental design was a randomized block design (RBD) with three replications. Five bermudagrass [Cynodon dactylon (L.) Pers.] cultivars, two teffgrass (Paspalum notatum Flugge) cultivars, and a Kleingrass [Panicum coloratum L.] cultivar were compared for forage production and quality in the middle region of Korea. After seeding, the numbers of days until seedling emergence for bermudagrass and kleingrass were observed at approximately day 11 and day 12, respectively. The heading dates of teffgrass and kleingrass were on July 12 and July 26, respectively. The dry matter (DM) yield of bermudagrass Tifton 85 was usually greater than the other entries. The crude protein content (CPC) and total digestibility nutrient (TDN) for the teffgrass cultivars were usually greater than the other entries at all study sites. The acid detergent fiber (ADF) and in vitro DM digestibility (IVDMD) were similar across all cultivars.
BACKGROUND: Biochar is used in various environmental fields, such as water quality and soil restoration, and affects soil fertility and nutrient cycling. Also, when crops are grown on biochar-applied soil, their characteristics may be affected. Biochar is used especially with commercial vegetable seedlings. METHODS AND RESULTS: The objective of this study was to determine the effects of biochar content in seeding mixes on early growth of lettuce (Lactuca sativa L.), Chinese cabbage (Brassica rapa L.), and red pepper (Capsicum annuum L.). Treatments consisted of a control (0: 10, ratio of biochar to seeding mixes (w/w)), 1: 9 (biochar 10%), 3: 7 (biochar 30%), 5: 5 (biochar 50%), and 7: 3 (biochar 70%). The biochar was made from risk husk and had a C/N ratio of 104. As the mixing ratio of biochar increased, pH increased whereas EC and nitrogen content decreased. The highest phosphorus content was with the treatment of 30% biochar, while there were significant increases in the weight of lettuce seedlings and concentrations of T-N, P2O5, K2O, MgO, and Na with the treatments of 30% and 50% biochar. Although the weight of Chinese cabbage seedlings increased with the treatment of 10% biochar, the increase was not statistically significant. Also, there was an increase in the weight of red pepper seedlings with the treatment of 30% biochar, but the increase was not statistically significant. With increases in the biochar mixing ratio, the K2O concentration of red pepper seedlings increased, but the concentrations of P2O5, CaO, MgO, and Na decreased. It was believed that this was because of absorption inhibition by calcium-phosphate formation in the seeding mixes owing to increased pH. CONCLUSION: In conclusion, adding biochar to seeding mixes is considered to be an important mean for growing healthy vegetable seedlings. More field experiments are needed to verify the effect of biochar on vegetable crop growth over the entire growing season.
Transparent vinyl-mulching cultivation plots heavy-fertilized (6-14-20 kg $10a^{-1}\; of\; N-P_2O_5-K_2O)$ in growing peanuts produced 315-344Kg $10a^{-1}$ of quality kernels through improvement of the low temperature conditions of underground environment during the early growing stage and kernel-filling stage. The yields of which were 82-99 percent higher than that of conventional cultivation plot. The vinyl-mulching cultivation techniques accelerated emergence, young seedling growth, flowering, pod bearing and kernel filling of peanuts in Korea.
Powdery mildew caused by Podosphaera fusca is one of the most important diseases in leafy lettuce (Lactuca sativa L.). Since the disease has been a threat to safe cultivation of leafy lettuce, its control methods have to develop to produce good quality of lettuce for farmer and consumer. Occurrence of lettuce powdery mildew is increasing more and more due to continuous cultivation of lettuce all through the year, non-removal of diseased plant parts of lettuce, spray of inadequate fungicides by mistaken acknowledge of lettuce powdery mildew for lettuce downy mildew, etc. The control effect of five fungicides against lettuce powdery mildew was examined in a plastic greenhouse located in Suwon. When fungicides were sprayed three times at 10 days-intervals in the early stage of occurrence of powdery mildew, the incidence of powdery mildew in the plants treated with kresoxim-methy SC, azoxystrobin SC, Ampelomyces quisqualis AQ94013 WP, Paenibacillus polymyxa AC-1 SC and Bacillus subtilis Y 1336 WP was 0.7%, 0.7%, 26.0%, 36.7% and 42.0%, respectively, whereas the incidence of non-treated control was 55.3% on eight days after final application. Phytotoxicity of five fungicides tested was not observed in lettuce seedling plants.
Kim, Dong-Won;Kim, Jong-Yeob;You, Dong-Hyun;Kim, Chang-Su;Kim, Hee-Jun;Park, Jong-Suk;Kim, Jeong-Man;Lee, Kang-Soo
Korean Journal of Medicinal Crop Science
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v.20
no.2
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pp.124-128
/
2012
When ginseng seedlings are cultured in paddy fields, quality degradation and yield reduction are induced by severe plant loss with chlorosis on leaves occurred physiological disorder by excessive salt and poor drainage, rusty-root occurrence, and root rot etc. Accordingly, in order to solve these problems, this study was performed to investigate the treatment method, concentrations and time of activated ion calcium as environment-friendly agricultural materials. Activated ion calcium is an enriched and purified water-soluble mineral calcium component for absorbing quickly into plant as a highly functional calcium and it is an alkaline calcium of 37% (370 $m{\ell}$/1 ${\ell}$) concentration with pH 13. Treatment method was that ginseng seeds were sown after removing water in the shade after seed immersion for 1 minute with active ion calcium of 20-fold diluted solution, and then irrigated $4{\ell}$ per 3.3 $m^2$ with 200-fold, 400-fold, and 600-fold diluted solution before emergence on late March, and supplied 1 time on leaves with 500-fold diluted solution in June and July respectively. The disease rate by treatment of activated ion calcium was that on the treatment of soil irrigated with 200-fold diluted solution compared to non-treated soil, damping-off was 33%, anthracnose was 100% reduced and the occurrence rate of rusty-root was 30% reduced. In addition, when active ion calcium of 200-fold diluted solution were soil irrigated, first and second grade ginseng were respectively 26% and 22% produced more, compared with control.
Grafting of fruit-bearing vegetables has been widely used to increase the resistance to soil-borne diseases, to increase the tolerance to low temperature or to soil salinity, to increase the plant vigor, and to extend the duration of economic harvest time. After grafting, it is important to control the environment around grafted seedlings for the robust joining of a scion and rootstock. Usually the shading materials and plastic films are used to keep the high relative humidity and low light intensity in greenhouse or tunnel. It is quite difficult to optimally control the environment for healing and acclimation of grafted seedlings under natural light. So the farmers or growers rely on their experience for the production of grafted seedling with high quality. If artificial light is used as a lighting source for graft-taking of grafted seedlings, the light intensity and photoperiod can be easily controlled. The purpose of this study was to develop a prototype system for the graft-taking enhancement of grafted seedlings using artificial lighting and to investigate the effect of air current speed on the distribution of air temperature and relative humidity in a graft-taking enhancement system. A prototype graft-taking system was consisted by polyurethane panels, air-conditioning unit, system controller and lighting unit. Three band fluorescent lamps (FL20SEX-D/18, Kumho Electric, Inc.) were used as a lighting source. Anemometer (Climomaster 6521, KANOMAX), T-type thermocouples and humidity sensors (CHS-UPS, TDK) were used to measure the air current speed, air temperature and relative humidity in a graft-taking system. In this system, air flow acted as a driving force for the diffusion of heat and water vapor. Air current speed, air temperature and relative humidity controlled by a programmable logic controller (UP750, Yokogawa Electric Co) and an inverter (MOSCON-G3, SAMSUNG) had an even distribution. Distribution of air temperature and relative humidity in a graft-taking enhancement system was fairly affected by air current speed. Air current speed higher than 0.1m/s was required to obtain the even distribution of environmental factors in this system. At low air current speed of 0.1m/s, the evapotranspiration rate of grafted seedlings would be suppressed and thus graft-taking would be enhanced. This system could be used to investigate the effects of air temperature, relative humidity, air current speed and light intensity on the evaportranspiration rate of grafted seedlings.
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