• Journal of Soil and Groundwater Environment
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• v.16 no.5
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• pp.9-17
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• 2011

Positive effect of multiple-PGPR (Plan Growth Promoting Rhizobacteria), isolated from heavy metal contaminated soil, on the germination of Barnyard grass (Echinochloa crus-galli var. frumentacea) was quantitatively estimated in 5 heavy metal (Cd, As, Ni, Cu, and Pb) contaminated liquid medium. The $EC_{50}$ value for respective heavy metal was estimated by TSK (Trimmed Speraman-Karber) model based on germination rate. The results showed overall increase in $EC_{50}$ with PGPR inoculation. The $EC_{50}$ value increased 1.4% from 96.0 mg/L (control) to 97.4 mg/L (PGPR-treated) in As contaminated medium. In Ni contaminated medium, the $EC_{50}$ value increased 31.9% from 148.0 mg/L (control) to 195.2 mg/L (PGPR-treated), while the $EC_{50}$ showed 4.8% increase from 63.4 mg/L (control) to 66.5 mg/L (PGPR-treated) in Cu medium. Overall seedling growth was stronger in the PGPR treated seeds than that in the control, but positive effect on seedling growth was not conspicuous. At effective concentration of 100 mg/L, the average seedling length of the PGPR treatment in As, Cd, Cu, and Ni medium, respectively, was 1.13, 0.14, 0.40, and 0.06 cm longer than that in the control. However, the increase of seedling growth was statistically insignificant (p < 0.05). These results suggest that inoculation of the isolated-PGPR exerts positive effects on seed germination by reducing heavy metal toxicity and can be an effective tool for application of phytoremediation on heavy metal contaminated soils.

• Korean Journal of Agricultural Science
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• v.23 no.2
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• pp.227-232
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• 1996

It is a tedious job to handle seedling trays in a green house with human hands because of their fluctuation when they are moving. A robotic gripper for handling a seedling tray has been developed., which has two fingers consisting of the vertical and horizontal bars. The maximum deflections at the center of the seedling trays were measured with various lengths of the horizontal bars. The length of 250 mm was revealed to be optimal one, resulting less than 15 mm deflection with a $540{\times}270mm$ seedling tray, which was acceptable for a practical use. For this study a LM system was adopted to move the fingers. To validate this system the robotic gripper was installed on a Cartesian robotic manipulator and their performance was tested several times with great success. The robotic manipulator used in this study was a general one, so a special one for this robotic gripper needs to be developed in future.

• Journal of Forest and Environmental Science
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• v.38 no.1
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• pp.38-47
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• 2022

Hymenodictyon orixensis (Roxb.) Mabberley (locally known as Bhutum in Bangladesh) is both an ecologically and economically valuable multipurpose tree species for afforestation and reforestation programs in Bangladesh. Seed germination and seedling development study of H. orixense were conducted to find out the response to different growing medium, e.g., polybag (15×10 cm (T₀) and 20×15 cm (T₁)), sand medium in propagator house (T₂), conventional nursery bed (T₃), and root trainer (T₄) in the Nursery. Consequently, germination behavior and seedling morphological parameters of H. orixense were assessed. The results revealed that the sand medium of the propagator house (T₂) provided the highest germination % (58.57±22.30) and the highest germination energy (11.43±2.43) followed by seedlings growing in 20×15 cm polybags (T1) containing forest topsoil and cow-dung at a ratio of 3:1. Except for germination energy, germination values, and germination capacity, other seed biology parameters, particularly imbibition, germination period, germination rate, and plant survival percent in T₁, T₂, T₃, and T₄ were significantly (p<0.05) different from T₀. Each phenotypic parameter of seedlings and dry matter of shoot and root significantly differed from control except root length (p<0.992). Based on this study, Polybags of 20×15 cm size are regarded as the best medium for quality seedling development of H. orixense. The nursery bed (T₃) had the lowest germination performance and developed more inferior quality seedlings. Thereby, 20×15 cm size of polybags with conventional soil and cow-dung media is recommended for maximum germination and to grow the quality seedlings of H. orixense in the Nursery.

• Ocean and Polar Research
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• v.44 no.4
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• pp.311-317
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• 2022

Seed germination and seedling growth of a halophyte, Suaeda japonica were examined to measure the effects of seed preservation periods and various soil compositions in the mudflat of Saemangeum, Korea. Seed germination declined from 74.30% to 44.30% as the preservation period increased from 4 months to 12 months. After 14 days of seeding, seed germination was lowest in sandy soil (SS) at 8.67% and highest in earthworm cast+leaf mold (EC+LM) at 67.33%, showing 4~5 times higher than SS. The average lengths of seedlings were 12.20 mm in SS and 42.20 mm in EC+LM, respectively. These results suggest that seed germination and seedling growth enhanced in the soil that has more organic matter. In the experiment mixing Saemangeum soil (SMS), seed germination was from 4.00% in SMS to 88.00% in the soil of SMS+EC+LM after 14 days. Similar growth patterns were observed in the length of seedlings from 5.00 mm in SMS to 49.60 mm in SMS+EC+LM. The present results indicate that a population of Suaeda japonica could be established by stimulating seed germination and seedling growth by the addition of organic matters such as EC, LM, and EC+LM in the Saemangeum mud flat.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.04a
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• pp.150-150
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• 2003

• Journal of Ecology and Environment
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• v.35 no.4
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• pp.343-349
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• 2012

The use of growth medium is often recommended milkweed seedlings to grow and develop after emergence, and it is affected by growth medium and local habitat conditions. The effects of growth medium and partial shade on early growth of milkweed under drought stress (Calotropis procera L.) were studied in a field experiment. A split-split plot experimental design with three replications was carried out in the nursery. The main treatment plot was divided into two levels of shade; (no shading and partial shading). Sub treatment plot1 included growth medium at four levels (G1 = clay [suitable for milkweed growth], G2 = clay + sand, G3 = clay + perlite, G4 = clay + perlite + sand) and sub treatment plot2 included drought (irrigation intervals) at six levels (D1 = 2 [control], D2 = 4, D3 = 6, D4 = 8, D5 = 10, and D6 = 12 days per for three month). The results showed that drought stress significantly decreased emergence percentage, shoot length, shoot dry weight (SDW1), root dry weight (RDW), seedling dry weight (SDW2) and vigor index (VI). The use of growth medium increased all seedling characteristics. The G3 (clay + perlite) growth medium showed the highest performance, especially in terms of emergence percentage and seedling dry weight. Partial shade improved shoot length, shoot dry weight, and vigor index. Our results showed that the best treatment for high-vigor milkweed seedlings under drought stress was G3 (clay + perlite) growth medium and partial shade.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.4 no.1
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• pp.25-29
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• 1968

The effect of differences in grain size on the initial growth of seedlings was studied in rice with two leading varieties of Jaekun and Nongkwang. The grains were classified into three classes of large, medium and small size by their specific gravity. The results observed at seedling stage are summarized as follows. 1. Higher percentage of seeds with larger size was observed to be normally developed into sound seedlings comparing with those with small seeds. This suggests the more effective maintainance of seedings. 2. Seedlings from larger seeds also showed higher values in the number of roots, leaves and tillerings where no varietal difference was observed. 3. Varietal speciality led to the lorger coleoptile of Nongkwang variety, with rather longer plant, than that of Jaekun, while higher value in coleoptile length was observed at the seedlings from larger seeds. 4. Thus larger seeds and the Aariety Nongkwang yielded heavier dry matter of upper part. These trends appeared more clearly at the late seedling stage. 5. There was constant decrease in the dry matter weight per unit coleoptile length until the seedling-selfsupportingstage while after this stage abrupt increase was observed. Seedlings from larger seeds showed higher value in the dry matter weight per unit coleoptile until this stage, thought the differences became insignificant after this stage. And no varietal difference was found.

• Journal of Bio-Environment Control
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• v.15 no.2
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• pp.149-155
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• 2006

This study was carried out to investigate effects of different night temperature treatments during nursery period on flower bud differentiation and growth of pepper cv. Cheongyang. Number of leaves, top fresh weight and top dry weight of pepper seedlings were increased with increasing the night temperature during nursery periods. And also flower bud differentiation and days to flowering were accelerated as increasing the night temperature. Plant height, stem diameter, branch length and intermode length of pepper after transplanting were height at the low night temperature ($28/11^{\circ}C$), but they were retarded at the high night temperature ($28/21^{\circ}C$) treatment. Number of lateral branches was significantly reduced at the high night temperature, but there was no a regular tendency in branching habit of the main stem by temperature treatments. Seedling growth before transplanting was retarded at the low night temperature but gradually recovered after transplanting into the plastic house. However, seedling growth at the high night temperature was shown in contrast to above response of the low night temperature.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.1
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• pp.122-127
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• 1983

Germination and seedling emergence of 40 barley cultivars and lines having been bred in Korea were studied under different water and salt stress conditions containing different sucrose and KC1 concentration in laboratory at Suweon. This study revealed that the barley cultivars and lines differed markedly in their emergence rate index(ERI), total stand(TS), coleoptile length, seedling height, top and root weight. Those characters of the most cultivars were progressively reduced as water and salt stress increased. Jogangbori, Dongbori 1, SB 77460 and SB 77415 were less sensitive to both stress and will be useful for breeding sources. ERI was highly correlated with TS, coleoptile length and seedling height. ERI and TS between two different solutions were correlated each other, and this study indicated that for screening the varieties or lines tolerant to water and salt stress, both ways were available and should be screened genetic materials with a good germination and emergence rate in I stress conditions.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.3
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• pp.319-323
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• 2013

Red rice (Oryza sativa L.) is one of the most important weeds in most of rice cultivating area. Seed germination related experiments were done to evaluate germination percentage, emerging speed, and initial growth by seeding depth and soil moisture level. The four experiments, 1) temperature, 2) seeding depth, 3) soil moisture level, and 4) seeding position, were done with combination each other on phytotron for germination and seedling growth related experiments of redrice. The treatments levels were: 1) Temperatures were 20/15(Low), 25/20 (Mid.), $30/25{\circ}C$ (High), (day/night), 2) seeding depths were 0, 3, 6 cm, 3) soil moisture levels were 25, 35, 45, 55, 65% (VWC, %), and seeding position were furrow, ridge, ridge-top. The germination percentage and germination speed of red rice were higher and faster than Daeanbyeo in low temperature. Yoeongcheon redrice of seed germination percentage and seedling length was more vigor than Hapcheon red rice. Red rice was not germinated on 6 cm seeding depth until 11 days after seeding except high temperature treatment. Germination percentage increased with increasing soil water percentage in low temperature, however it was greatest in 45% in high soil moisture level between 25% to 65% in low temperature. Seed germination percentage and seedling length were not significantly different among the soil water level in mid- and high temperature levels. In conclusion, red rice could germinate in top soil (<6 cm) in mid- and high temperature range, so we might be control red rice by spraying herbicide after germination of red rice combined with delayed rotary cultivation.