• Title/Summary/Keyword: Seedling Growth

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Repellent and Insecticidal Activity of Sequential Extracting Fractions Obtained from BPH-Resistant Rice Varieties against Brown Planthopper (Nilaparvata lugens) (벼멸구 저항성벼 품종 추출분획물의 기피 및 살충 활성)

  • Kim, Sung-Eun;Kim, Young-Doo;Kim, Bo-Kyoung;Ko, Jae-Kwon;Chun, Jae-Chul
    • The Korean Journal of Pesticide Science
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    • v.10 no.2
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    • pp.124-130
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    • 2006
  • Rice plant extracts of brown planthopper (BPH) resistant rice varieties, Jangseongbyeo (JSB) and Hwacheongbyeo (HCB) at different growth stages (seedling, tillering, heading and ripening) were sequentially fractioned using hexane, ethyl ether, ethyl acetate, butanol, and distilled water. The extracts were applied to BPH susceptible rice variety, Dongjjnbyeo (DJB), to investigate the insecticidal and repellent effects against BPH. BPH insecticidal effects were not clearly observed with almost all of the extract fractions obtained from both JSB and HCB varieties for 12 h, whereas the ethyl ether and hexane extract fractions showed about 10 to 30% of BPH mortality in 24 to 48 h of application periods. An effective BPH repellent activity was found with the applications of ethyl ether extract fractions obtained from JSB variety. The extract fractions obtained from HCB variety did not show any different repellence among the various fractions. The BPH repellent effects of the extract fractions obtained at different growth stages of either JSB or HCB varieties did not show any correlations. The effect of ethyl ether fraction on BPH repellent was continually increased by 30 h after treatment and thereafter decreased. In addition, the first sub-fraction separated by a flash column chromatography eluted with chloroform:methanol (9:1, v/v) from the BPH effective ethyl ether faction in JSB variety might be meaningful to repel BPH from BPH susceptible target rice plants. The results indicated that the ethyl ether fraction obtained from JSB was higher in repellent activity than in insecticidal activity, and suggesting that there might be specific substance(s) in the first sub-fraction (sF1) of the ethyl ether fraction in JSB that could provide repellent activity against BPH.

Production of Organic Rice (Oryza sativa L.) using Organic Cultivation Manual (유기재배매뉴얼을 활용한 유기쌀 생산)

  • Cha, Kwang-Hong;Oh, Hwan-Jung;Seo, Dong-Jun;Song, Yong-Su;Ahn, Joon-Seob;An, Kyu-Nam;Jung, Woo-Jin
    • Korean Journal of Organic Agriculture
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    • v.22 no.1
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    • pp.97-113
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    • 2014
  • An actual proof experiment was carried out to establish the production of high quality organic rice at farm household in Gwangjin, Junnam, Korea 2011. Experimental plot was designed with conventional organic cultivation and manual organic cultivation in both Hopeongbyeo and Onnuri varities. Experimental field was consisted with suitable drainage loam soil, anryong series, and normal paddy field. Chemical characteristics of the soil were investigated from initial soil and soil after harvest in experimental sites. Seed disinfection was treated with hot water dipping method at $60^{\circ}C$ for 10 min. Rice transplanting was carried out at 17 June with 25 days seedling using machine transplanting. Golden apple snail was used to prevent weed growth as middle size (1.5~1.7 kg/10a) at one week after rice transplanting. Amount of applied fertilizer in experimental plot was supplied with organic fertilizer for the shortage after harvest of hairy vetch (1,100 kg/10a). Bordeaux mixture was used to control of sheath blight and neck blast disease in all experimental plot at 19 Aug. Application of chitin culture solution was treated in manual organic cultivation at 2 and 15 Aug. At late growth stage, incidence rate of neck blast was the highest level at Hopyeongbyeo in conventional organic cultivation. Incidence rate of sheath blight in both Hopyeongbyeo and Onnuri was higher in conventional organic cultivation than in manual organic cultivation. Incidence of rice leaf folder was the highest level at Onnuri in conventional organic cultivation. Yield index in manual organic cultivation was increased by 13% and 38% at Onnuri and Hopyeongbyeo, respectively, compared with conventional organic cultivation. Head rice percentage in both Hopyeongbyeo and Onnuri was slightly increased in manual organic cultivation. These results indicate that high quality production of rice organic cultivation could be depending on management of safe fertilization with nutrient content in soil.

Determination of optimum gamma ray range for radiation mutagenesis and hormesis in quinoa (Chenopodium quinoa Willd.)

  • Park, Chan Young;Song, Seon Hwa;Sin, Jong Mu;Lee, Hyeon Young;Kim, Jin Baek;Shim, Sang In
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.240-240
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    • 2017
  • Quinoa (Chenopodium quinoa Willd.) is one of the ancient crops cultivated in the Andes region at an altitude of 3,500-4000m in Chile and Bolivia from 5000 BC. It contains a large amount of protein, minerals and vitamins in comparison with other crops. The cultivation area has been increasing worldwide because of its excellent resistance to various abiotic stress such as salinity, drought and low temperature. ${\gamma}$-Ray radiation of high dose is often used as a tool to induce mutations in plant breeding, but it has a deleterious effect on organisms. However, the radiation may have a positive stimulatory effect of 'hormesis' in the low dose range. This experiment was carried out to investigate the optimum dose range for creating the quinoa genetic resources and to investigate the hormesis effect at low dose on the quinoa. This experiment was performed for 120 days from November, 2016 to February, 2017 in the greenhouse of Gyeongsang National University. ${\gamma}$-Ray radiation was irradiated to seeds at 0 Gy, 50 Gy, 100 Gy, 200 Gy, 300 Gy, 400 Gy, 600 Gy, 800 Gy and 1000 Gy for 8 hours. (50 Gy) using the low level radiation facility ($Co^{60}$) of Cooperative Research Institute of Radiation Research Institute, KAERI. Fifty seeds were placed on each petri dish lined with wet filter paper and germination rate was measured at a time interval of 2 hours for 40 hrs. The length of the root length was measured one week after germination. Each treatment was carried out in 3 replicates. The growth of seedlings were investigated for 10 days after transplanting of 30 day-old seedlings. The plant height, NDVI, SPAD, Fv/Fm, and panicle weight were measured. The germination rate was highest at 50Gy and 0Gy and the rate of seeds treated with 400Gy or higher rate decreased to 25% of the seeds treated with 50Gy. The emergence rate of seedling in pot experiment was higher at the dose of 200 Gy, 300 Gy and 400 Gy than at 0 and 50Gy. However, the rate was lower at strong radiation higher than 600Gy at which $1^{st}$ leaf was not expanded fully and dead due to extreme overgrowth at 44 days after treatment (DAT). The highest value of panicle weight was observed at 50Gy (6.15g) and 100Gy (5.57g). On the other hand, the weight at high irradiated dose of 300Gy and 400Gy was decreased by about 55% compared to low dose (50 Gy). NDVI measurement also showed the highest value at 50 Gy as the growth progressed. SPAD was the highest at 400 Gy and showed positive correlation with irradiation dose except 0 Gy. Fv/Fm was high at 50 Gy up to 30 DAT and no difference between treatments was observed except for 400 Gy from 44 DAT. The plant height was the highest in 50Gy during the growing period and was higher in the order of 50Dy, 100Gy, 0Gy, 200Gy, 300Gy and 400Gy in 88 DAT. In this experiment, the optimal radiation dose for hormesis was 50Gy and 100Gy, and the optimal radiation dose for mutagenesis seems to be 400 Gy.

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The Study of Genetic Diversity for Drought Tolerance in Maize (옥수수 한발 내성에 관한 유전적 다양성 조사)

  • Kim, Hyo Chul;Lee, Yong Ho;Kim, Kyung-Hee;Shin, Seungho;Song, Kitae;Moon, Jun-Cheol;Lee, Byung-Moo;Kim, Jae Yoon
    • Korean Journal of Environmental Biology
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    • v.34 no.4
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    • pp.223-232
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    • 2016
  • Drought is one of important environmental stress for plant. Drought has deleterious effect to plant growth including maize (Zea mays L.) such as vegetative and/or reproductive growth, root extension, photosynthesis efficiency, flowering, anthesis-silking interval (ASI), fertilization, and grain filling. In this study, we screened drought tolerant maize in 21 cultivars from different sources, sixteen NAM parent lines (B73, CML103, CML228, CML247, CML277, CML322, CML333, CML69, Ki11, Ki3, Ky21, M37W, Mo18w, NC350, Oh43 and Tx303), four Korean hybrids (Cheongdaok, Gangdaok, Kwangpyeongok and Pyeonganok) and one Southeast Asian genotype (DK9955). Drought stress (DS) index was evaluated with leaf rolling score at seedling stage and ASI at silking date. The leaf rolling scoring of CML228, DK9955 and Ki11 were determined 1.28, 1.85, 1.86, respectively. However, M37W, Kwangpyeongok, B73 and NC350 were determined over the 3. ASI analysis revealed that CML228, CML103, Cheongdaok, NC350, B73, CML322, Kwangpyeongok and Ki11 are represented less than 5 days under DS and less than 3 days of difference between DS and well-watered (WW), but CML69, Ki3, Pyeonganok, M37W, Mo18w and Gangdaok were represented more than 10 days under DS and more than 8 days of difference between DS and WW. Multi-Dimensional Scaling (MDS) analysis determined CML228, Ki11, and CML322 were regarded as drought tolerance cultivars. Eventually, Ki11 showed genetic similarity with Korean cultivars by QTL analysis and MDS analysis. Ki11 has a potential for development of drought tolerance maize with Korean cultivars.

Influence of Toxic Heavy Metals on Germination of Rice Seeds and Growth of Rice Seedling (수도생육(水稻生育)에 대한 유해(有害) 중금속(重金屬)의 영향(影響) - 발아 및 묘대기(苗垈期) 생육(生育)에 대하여 -)

  • Kim, B.J.;Ha, Y.L.;Kim, J.O.;Han, K.H.
    • Korean Journal of Soil Science and Fertilizer
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    • v.11 no.2
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    • pp.119-126
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    • 1979
  • Rice seeds, suweon 264, were germinated under 5 levels of toxic heavy metals, Cd(0, 0.05, 0.5, 5, 20ppm), Cu(0, 0.05, 0.5, 5, 20, ppm), Cr(0, 0.5, 1, 5, 10ppm), Ni(0, 0.5, 1, 5, 10ppm), Co(0, 0.5, 1, 5, 10ppm), Zn(0, 0.5, 5, 20, 40, ppm), Pb(0, 0.5, 5, 20, 40ppm) and Mn(0, 1, 10, 25, 50, ppm) in culture solution, and then grown with supplying culture solution contained respective concentrations. Germination and growth response to the toxic heavy metals were studied. Results obtained are as follows : 1) The germination injury of rice seeds by excess concentration of toxic heave metal in culture solution occured in Cd and Cu; below 0.05 ppm, Ni; below 0.5 ppm, Mn; below 1.0 ppm, Co and Cr; 0.5-1.0 ppm, and 0.5-5 ppm, Zn and Pb. Thereby, in the order of degrees of the elements toxicity to germination, they were arranged as follows : Cd>Cu>Ni>Co>Cr>Mn>Zn$$\geq_-$$Pb. 2) Toxic heavy metal concentrations in culture solution, which result in decreasing dry weight due to the injury of excess concentration of treated elements, were Cd: below 0.05 ppm, Ni, Cr and Co; below 0.5 ppm, Cu and Zn; 0.5-5 ppm, Pb; 5-20 ppm and Mn; 10-25 ppm. The order was Ni>Cd>Cr>Co>Cu$$\geq_-$$Zn>Pb>Mn. 3) The critical contents of Cd, Ni, Pb, Cu, Zn, Mn, and Co in dry matter, Which result in decreasing dry weight, were considered to be 0.05-15.5, 1.50-25.0, 24.0-28.0, 26.5-62.5, 470-645.0, 231.0-500.0 and below 15.0 ppm, respectively. 4) The contents of Cr, Co, Cd, Pb, Ni, Cu and Zn in dry matter by 0.5 ppm treatment concentration of each heavy metals was trace, 15.0, 17.5, 24.0, 25.0, 84.5 and 470.0 ppm, respectively. Thereby, in the order of each element to uptaked by rice seedlings, they were arranged as follow; Zn>Cu>Ni>Pb>Cd>Co>Cr. 5) The hazardous concentrations of root activity by toxic heavy metals in culture solution were Cd; below 0.05, Cu; 0.05-0.5, Cr; below 0.5, Ni; 0.5-1.0, Co; 0.5-1.0, Zn; above 0.5, Pb; 0.5-5.0 and Mn; 1.0-10.0 ppm. The hazardous degree of root activity by toxic heave metals was in the order of Cd>Cu>Cr>Zn>Ni>Co>Pb>Mn.

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Morphological and Anatomical Response of Rice and Barnyardgrass to Herbicides under Various Cropping Patterns - I. Response to Pyrazolate (재배양식(栽培樣式)에 따른 수종(數種) 제초제(除草劑)에 대한 벼와 피의 해부형태적(解剖形態的) 반응차이(反應差異) - I. Pyrazolate에 대한 반응차이(反應差異))

  • Chon, S.U.;Guh, J.O.;Kuk, Y.I.
    • Korean Journal of Weed Science
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    • v.15 no.1
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    • pp.30-38
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    • 1995
  • Soil-applied pre-emergence herbicide, pyrazolate(4-(2, 4-dichlorobenzoyl)-1, 3-dimethyl pyrazol-5-yl-p-toluene sulphonate) induced, twist effect of shoots of barnyardgrass under dry conditions, and etiolated leaf and stem of that under water condition. Plant height and root length of rice broadcast on soil surface were similar to the untreated control, but plant height of rice drilled in soil was more inhibited than root length as compared with the untreated control, while development of barnyardgrass seedling was severely inhibited at 20 days after application. The inhibition rate was much higher under water condition than under dry condition, but difference in rice and barnyardgrass did not abserve. However, growth of transplanted rice shown to increase to the untreated control. Shoot and root fresh weight of rice broadcast on soil surface was increase as compared with the untreated control, and that of rice drilled in soil was not affected whereas that of barnyardgrass was severely inhibited by 42% and 41%, respectively. Under dry condition at 20 days after pyrazolate application while root growth of rice broadcast on soil surface under water condition was deadly inhibited and development of barnyardgrass was almost completely inhibited. On the other hand, microscopic studies showed that constriction of mesophyll cell by destruction of chloroplast of barnyardgrass were occurred only under dry condition, whereas damage of rice and barnyardgrass under water and transplanting condition were not observed. Anatomical change in the meristernatic region of rice and barnyardgrass was not occurred, and similar to intact plant regardless of cropping patterns.

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Effect of Nursery Stage and Plug Cell Size on Growth and Yield of Waxy Corn (이식재배가 찰옥수수 생육 및 수량에 미치는 영향)

  • Kim, Sung-Kook;Jung, Tae-Wook;Lee, Yu-Yong;Song, Duk-Yong;Yu, Hong-Seob;Lee, Choon-Woo;Kim, Yee-Gi;Lee, Jae-Eun;Kwak, Chang-Gil;Jong, Seung-Keun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.1
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    • pp.24-30
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    • 2010
  • Comprehensive studies on seedling production and transplanting cultivation of waxy corn are necessary to fulfill the comsumer's preference for the high quality whole ear waxy corn and its year-round supply for higher price, and to escape marketing in a short period at harvest season. Two waxy corn hybrids (cv. Chalok# 1 and Chalok# 4) were used to study the effects of seeding date, nursery days and plug cell size on growth and yield of waxy corn, and to clarify the reliable transplanting date at the Experimental Field of the National Institute of Crop Science in 2007. The number of days from seeding to silking was increased as nursery days were extended, but the number of days from transplanting to silking was shortened comparing to the direct seeding. Number of days from seeding to silking was shorter by 9~12 days for Chalok# 1 than for Chalok# 4, while the number of days from seeding to silking was shorter by 12~15 days in the second cropping than in the first cropping. Culm length of transplanted waxy corns was decreased as the nursery days were extended. Culm length of seeded Chaok# 1 and Chaok# 4 were decreased by 17% and 24%, respectively, in the second cropping compared to those in the first cropping. Ear length was somewhat decreased as the nursery days were extended. Chalok# 1 in the second cropping the degree of decrease was much higher in second cropping than in first cropping of Chalok# 1. Comparing to the first cropping, number of marketable ears per 10a of Chalok# 1 decreased as high as 64%, while that of seeded Chalok# 4 decreased mere 12% in the second cropping.

Selective Mechanism of Cyhalofop-butyl ester between Rice and Echinochloa crus-galli - I. Differential Response of Rice and Echinochloa crus-galli to Cyhalofop-butyl ester (제초제(除草劑) Cyhalofop-butyl ester의 벼와 피간(間) 선택성기작(選擇性機作) - I. 제초제(除草劑) Cyhalofop-butyl ester에 대한 벼와 피간(間)의 반응차이(反應差異))

  • Park, J.E.;Ryu, G.H.;Lee, I.Y.;Lee, H.K.;Shin, H.S.;Lee, J.O.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.14 no.2
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    • pp.94-100
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    • 1994
  • This experiment was conducted to determine selective mechanism of cyhalofop-butyl ester ((((R-butyl 2-(4-(4-cyano-2-fluorophenoxy) phenoxy) propionate)) between rice and Echinochloa crus-galli. 100ppm of cyhalofop-butyl ester inhibited over 90% of seedling growth of E. crus-galli when applied at 3 leaf stage and complete inhibition was observed at 180ppm applied at the 4 leaf stage, but rice(Chucheongbyeo) was not inhibited by cyhalofop-butyl ester even at 230ppm, regardless of its growth stages(3, 4, 5 and 6 leaf stages). Cyhalofop-butyl ester applied through stem at 10 and 50ppm moved most rapidly to the meristem and resulted in the highest injury on plant height, root length and fresh weight of E. crus-galli. compared with root or leaf application. Seedlings of rice and E. crus-galli at 3 or 4 leaf stage were dipped in 180ppm of cyhalofop-butyl ester solution for 1 minute and aboveground parts of E. crus-galli and rice were removed immediataly after dipping treatment. Regrowth of E. crus-galli was inhibited by the herbicide by 41.7%, but no inhibition was observed in rice. Further, content of chlorophyll reduced to 18.7% of the untreated control, showing appearence of almost being killed, but no effect on chlorophyll content of rice was observed.

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Comparison of Several Agronomic Characteristics as Affected by Seeding Dates (파종기 이동이 맥류의 실용적 저형질에 미치는 영향)

  • Hyung-Soo Suh
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.4
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    • pp.298-303
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    • 1981
  • A study was conducted to find out the varietal difference in some important agronomic characters in response to different seeding time in barely from 1975 to 1976 at Chikugo Agric. Expt. Station in Japan. Thirteen varieties which are diverse in growth period were used. Nine seeding time were tried. Seedling emergence, primary tillering, and formation of flower primodia were delayed in proportion to the seeding time. And also heading and maturing time showed the same tendency. This tendency appeared more conspicuous in the late maturing varieties. Therefore, days to heading and maturing duration were inversely shortened by the delay of seeding time and by earliness of variety. Number of spikes as well as culm length and spike length was reduced in the late seeding. Grain yield was reduced in all varieties by late seeding, easpecially with late maturing varieties. In this test, widely adaptable variety to late seeding time was not found, but earliness and high tillering in growth habit appeared more favorable to yield increase.

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Effect of Nonwoven Fabrics Weight on the Growth, Quality and Yield of Oriental Melon (Cucumis melo L. var makuwa Mak.) (보온부직포 무게가 참외의 생육, 품질 및 수량에 미치는 영향)

  • Shin, Yong-Seub;Yeon, Il-Kweon;Kim, Jwoo-Hwan;Park, So-Deuk;Kim, Byung-Soo
    • Journal of Bio-Environment Control
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    • v.14 no.2
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    • pp.89-94
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    • 2005
  • This study was conducted to investigate the effect of nonwoven fabrics weight(Ounce) on growth, quality and yield of oriental melon (Cucumis melo L. var. makwa Mak.). Seedling of 'Gumssaragi-Eunchun' was grafted on 'Shinthozoa' root stock. In this study, The minimum air temperature in 6 ounce at night was $4.8^{\circ}C,\;6.9^{\circ}C$ in 9 ounce, $7.9^{\circ}C$ in 12 ounce and $8.0^{\circ}C$ in 15 ounce, respectively. Leaf length, stem diameter, leaf numbers, leaf area, fresh weight and dry weight for 30 days after planting were better in high minimum air temperature at night than in low minimum air temperature, particularly leaf areas of the plant in 9 ounce was $370cm^2$ at 30 days after planting, $116\%$ in 12 ounce, $129\%$ in 15 ounce. The xylem exudates amount in 9 ounce for 24 hours just after basal stem abscission was 10.1mg. It was 1.2 times much in 12 ounce and 1.9times much in 15 ounce than in 9 ounce at 30 days after planting. The blooming of female was faster by 6 days in 15 and 12 ounce but was delayed by 3 days in 6 ounce than 9 ounce, and the days of blooming to harvesting were shorter by 3 days in 15 ounce and 4 days in 12 ounce but was delayed by 3 days in 6 ounce than in 9 ounce. Fruit weight, fruit length, fruit diameter, flesh thickness, soluble solids, and total yield was the highest in 15 ounce followed by 12 ounce,9 ounce and 6 ounce. Fermented fruit rate was the highest in 6 ounce followed by 9 ounce, 12 ounce and 15 ounce, and marketable fruit rates were 15, 12, 9 and 6 ounce in order. Compared to 1,781kg yield per 10a of 9 ounce, $19\%\;and\;49\%$ was increased under 12 ounce and 15 ounce but $47\%$ were decreased 6 ounce.