• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.232-237
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• 2003

Benzyladenopuyine (BA) commonly used for soybean sprout production is of high price. The study was carried out to determine the effect of BA treatment time during seed imbibition on growth and development of soybean sprouts and to analyse its treatment cost. The soybean seeds of 4 cultivars were soaked in 4 ppm BA solution during the first 5.5 hour imbibition (EFHI), the second 5.5 hour imbibition (SFHI) immediately after 0.5 hour aeration, or whole 11.0 hour imbibition (WFHI) intervened by aeration. On the 6th day after culture, the soybean sprouts were classified into 4 categories on the base of hypocotyl length; ＞ 7cm, 4 to 7cm, ＜ 4 cm and not germinated, and their morphological characters, fresh and dry weights were measured. Water absorption of the seeds was sharply reduced after it was almost done for the first 5.5 hours. The percentage of sprouts with hypocotyls of longer than 4cm was higher in FFHI treatment than in the other two ones. Regardless of BA imbibition time and periods, the lateral roots were not observed. WFHI treatment showed shorter hypocotyl and root lengths but thicker hypocotyl and hook diameters than FFHI and SFHI treatments. All component fresh and dry weights except cotyledon fresh weight were nearly same. Treatment cost of BA was the lowest in SFHI treatment. It is concluded that BA treatment during SFHI is the best time because its treatment time did not affect sprout growth but its treatment cost.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.1
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• pp.73-78
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• 2017

This study was conducted to elucidate the effects of mulching materials on root growth characteristics and weed occurrence of Platycodon grandiflorum transplants (3 years old) in organic farming. After transplanting, roots were mulched with one of three mulching materials (biodegradable film, sawdust, or rice husks) or a control (no mulching). The average root diameter of all mulching groups was 28.1 mm, which was 5.1 mm thicker than the no-mulching treatment. Roots with the biodegradable film treatment were the thickest at 30.9 mm. Root length was the shortest, 22.0 cm, with rice husk mulching. The number of rootlets was the highest with film mulching (36.0 rootlets/root) and the lowest with no mulching. For root fresh weight, which directly affects yield, the average of treated and untreated roots was 100 and 56 g/root, respectively. The highest fresh weight was in the biodegradable film plot (130 g/root). The mulching treatment groups showed a weed inhibition effect compared with the no-mulching treatment. The dominant weeds with the no-mulching treatment were Setaria viridis and Digitaria ciliaris, and that with the mulching treatment was Setaria viridis. Average annual weed occurrence across all treatments was 72%. Weed occurrence was the highest with the no-mulching treatment ($125weeds/m^2$), and there were no weeds with the biodegradable film treatment.

• Journal of Bio-Environment Control
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• v.24 no.3
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• pp.196-201
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• 2015

This study was conducted to seek optimum sowing time and pinching height for tender shoots production of moloheiya (Corchorus olitorius L.), which is a subtropical leaf vegetables, under an unheated greenhouse in the south of Korea. Seeds of moloheiya did not germinate at all at $10^{\circ}C$, but germinated well at $18{\sim}30^{\circ}C$ with 95.5~98.5% germination rate. Germination rate was 75.0% at $15^{\circ}C$, but germination speed was very low. The pods of moloheiya sown before April 28 was matured after late-August, but those sown after May 7 was not matured in that year. In tender shoot production and harvest frequency, treatment sown on April 14 was most plentiful among sowing time treatments, and showed marketable yield increase by 14~42% compared to other sowing times. Mean-while, pinching at 100cm high from soil surface was more effective than other heights for increment of branches and tender shoot. All above suggest that it was suitable to sow seed on mid-April and to pinch at 100cm high from soil surface for increment of tender shoots in the south of Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.8
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• pp.1080-1087
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• 2004

A trial with twenty-four newly weaned Awassi lambs (initial body weight=21.5$\pm$0.8 kg) was conducted using a 3$\times$2 factorial design to study the effect of feeding three sources of protein supplements (soybean meal (SBM), sunflower seed meal (SSM), and cottonseed meal (CSM)), either untreated or formaldehyde-treated on the growth performance and carcass traits of Awassi lambs. Lambs were randomly assigned to one of the six diets (4 lambs/treatment diet) and were individually fed for a period of 107 days. Experimental diets were isonitrogenous and isocaloric. Final live weight and average daily gain (ADG) were affected by both source of protein and formaldehyde treatment (undegradable protein). Lambs fed untreated diets had better (p<0.01) daily gain compared to those fed formaldehyde-treated diets. Similarly total feed intake per animal was significantly (p<0.05) affected by protein source and formaldehyde treatment. Formaldehyde treatment caused a significant decrease (p<0.01) in feed intake compared to lambs fed untreated diets. Feed requirement per unit of gain was not affected by formaldehyde treatment during all periods of the experiment except for the second period (the second 28 day period), whereby untreated SBM, SSM and CSM had better feed conversion ratio (FCR) than the treated groups. Source of protein had a moderate effect (p<0.10) on FCR but had a significant effect (p<0.05) on hot and cold carcass weight, digestive tract empty weight and liver weight, with lambs fed SBM having higher values than lambs fed SSM and CSM diets. Supplementation with undegradable protein had a significant effect (p<0.05) on dressing-out percentage (p<0.05), final live weight, and hot and cold carcass weight (p<0.01). The lower values pertain to lambs fed treated diets compared to lambs fed untreated diets. In general, there were no significant differences among all carcass linear dimensions, carcass cut weights and dissected loin tissue weights for both treatments (protein source and formaldehyde treatment). Supplementation with undegradable protein but not the source of protein resulted in significantly higher dissected leg total bone weight (p<0.05), tibia and femur weight (p<0.05), and femur length (p<0.01) at the same carcass weight. Results suggest that the treatment of SBM, SSM and CSM with formaldehyde did not improve efficiency of feed utilization, lamb performance or carcass traits and that the SBM diet resulted in an increase in lamb performance compared to other experimental diets.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.1
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• pp.29-35
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• 2003

In order to find the feasibility of feeding raw or processed cotton (Gossypium) seed meal (CSM), 30 male crossbred lambs were randomly assigned to 5 dietary treatments with 6 animals each. Lambs were fed each of the isonitrogenous and isocaloric concentrate mixtures containing 30% deoiled groundnut cake (DGNC) (reference diet), 40% raw, 45 minute cooked, 1% $Ca(OH)_2$ or iron (1 part free gossypol:0.3 parts iron) treated CSM (replacing about 50% nitrogen moiety of reference concentrate mixture). The concentrate mixture was fed to meet 80% of CP requirements (NRC, 1985) along with ad libitum maize (Zea mays) hay for 180 days. Incorporation of raw or processed CSM did not affect the palatability of diets as evidenced by higher (p<0.01) or comparable overall daily intakes of DM and protein (CP and Digestible CP) per kg $W^{0.75}$ by lambs in comparison to reference group. Intakes of DE and ME increased (p<0.01) due to inclusion $Ca(OH)_2$ treated CSM in diets, while the intakes were lower (p<0.01) on iron treated CSM based diet when compared with reference diet. The intakes of DM, CP, TDN, DE and ME by reference and experimental lambs were higher than the requirements stipulated by NRC (1985). The growth rate was highest (p<0.01) in lambs fed on diets with cooked CSM followed by those fed raw or $Ca(OH)_2$ treated CSM based diets. The growth of lambs fed on iron treated CSM diets was depressed (p<0.01) in comparison to that of other experimental lambs probably due to increased iron intake (889 mg/kg DM of diet) which was much higher than the toxic levels of 500 ppm (NRC, 1980). The lambs on various diets utilized DM with similar efficiency while the utilization of energy (TDN, DE and ME) was more efficient (p<0.01) when the diets contained raw or cooked CSM in comparison to that of reference diet. The lambs fed raw and $Ca(OH)_2$ treated CSM incorporated diets utilized DCP with higher (p<0.01) efficiency than the lambs fed reference diet. The feed cost per kg weight gain was lower (p<0.01) on raw, cooked and $Ca(OH)_2$ treated CSM based diets in comparison to reference diet. Cooking of CSM for 45 minutes further reduced the feed cost of weight gain.

• Food Science and Preservation
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• v.13 no.3
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• pp.273-278
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• 2006

In order to help the preservation of the cherry tomato and unshiu orange, antimicrobial paper incorporating grapefruit seed extract and zeolite was applied to the package. Cherry tomato and unshiu orange were packed in a box (38x25x20 cm) attached with antimicrobial paper and then stored respectively at $5^{\circ}C$. During the storage, weight loss, pH total acidity, soluble solid content microbial load and decay ratio were measured as quality indices. pH increase in cherry tomato was observed until 20 days, and decreased with litle difference between the packaging treatments thereafter pH and total acidity decrease in unshiu orange were shown till 30 days, and abrupt change was revealed by 40 days. This was due to physiological disorders. The microbial loads of total aerobic bacteria, and yeast/mold count were suppressed during storage by the box packaging incorporated with antimicrobial agents, which also contributed to reducing the decayed cherry tomato and unshiu orange. Antimicrobial paper was useful fur the reduction of microbial load in cherry tomato and unshiu orange pear without other quality deterioration.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.628-635
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• 2011

This study was conducted to establish the tissue culture system for Korean domestic Miscanthus sinensis, which is used in various purposes such as forage, and bio-energy resources. With the mature seed of Miscanthus, optimum concentrations of plant growth regulators were identified for an efficient callus induction and regeneration. Among the treatments of 1~10 $mg{\cdot}L^{-1}$ 2,4-D, IBA, or NAA, callus induction rate was highest (85.3%) on MS medium containing 5 $mg{\cdot}L^{-1}$ 2,4-D. Under the condition, the callus were efficiently induced and proliferated with comparably lower frequencies of callus browning. In shoot regeneration, the treatment of NAA combined with BAP seemed to contribute more efficient conditions to shoot regeneration than those of NAA with Kinetin or 2-iP. Especially, regeneration efficiency and number of regenerated plants were 83.7% and 5.5 in 3 $mg{\cdot}L^{-1}$ NAA with 5 $mg{\cdot}L^{-1}$ BAP, respectively, which were higher frequencies than those in NAA with Kinetin or 2-iP. In results, 5 $mg{\cdot}L^{-1}$ 2,4-D and 3 $mg{\cdot}L^{-1}$ NAA combined with 5 $mg{\cdot}L^{-1}$ BAP were efficient for embryogenic callus induction and regeneration of Miscanthus. This system would be useful for mass-propagation and developing new cultivars via tissue culture of Miscanthus sinensis.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.80-80
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• 2018

Hosta is a genus of the family Asparagaceae and distributed in East Asia. There are six Hosta species (Hosta capitata (Koidz.) Nakai, H. clausa Nakai, H. jonesii M.G.Chung, H. minor (Baker) Nakai, H. venusta F.Maek., and H. yingeri S.B.Jones) native to Korea and among them, four species (H. minor, H. jonesii, H. venusta and H. yingeri) are endemic to the Korea peninsula. Hosta is generally propagated by seed, crown division or tissue culture. However, tissue culture is a more efficient method to mass proliferation, a new cultivar development and disease-free plantlet production in a limit time. Hence, we conducted this study to evaluate the influence of various plant growth regulators (PGRs) treatments on the induction of callus and somatic embryo of the six Hosta species. Leaf, petiole and root were used to select optimum tissue culture explants. Petiole explants thus only were used for callus induction and somatic embryogenesis with TDZ (0.1, 0.5 or 1.0mg/L) and NAA (0.1 or 0.5 mg/L) combinations. After 12 weeks of culture, the highest rate of somatic embryogenesis was achieved on modificated MS medium containing 1.0 mg/L TDZ and 0.1 mg/L NAA in H. capitata and H. minor (15.5%, respectively), 0.1 or 0.5 mg/L TDZ and 0.1 mg/L NAA in H. jonesii (22.2%), 1.0 mg/L TDZ and 0.5 mg/L NAA in H. yingeri (26.7%), and 0.1 mg/L TDZ and 0.5 mg/L NAA in H. venusta (53.3%). H. clausa showed very low effect on somatic embryogenesis by PGRs; 2.2%. There was interspecies difference to PGRs respond for callus and somatic embryo induction. Regenerated multiple shoots and plantlet of H. minor, H. jonesii, H. venusta and H. yingeri were obtained via somatic embryogenesis.

• Korean journal of applied entomology
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• v.26 no.4 s.73
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• pp.251-255
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• 1987

This study was conducted to investigate the effects of cultural methods on infection by Rhizoctonia disease(Rhizoctonia solani $K\"{u}hn$) of potato (Solanum tuberosum L.). Two potato cultivars, i.e., Irish Cobbler and Superior were planted in 1984 at the Alpine Experiment Station and 4 cultural methods were used in Combinations or separately. The deeper planting showed the later germination of sprout. The degree of infection on underground stems didn't show any differences between the treatments, but the yield was higher in deeper planting. When seed tubers were planted on April 28 the underground stems showed severely discolourated epidermis comparing to later planting, consequently the yield was low. The glazed chitting tubers produced healthy sprouts comparing to the untreated tubers, consequently the yield was also increased. The density of sclerotia on tuber skin was remarkably increased between 6 to 15days of delayed digging after haulm killing. Underground stems of Irish Cobbler were easily discolourated by the disease, where-as the density of sclerotia was higher on tuberskin of Superior than of Irish Cobbler.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.1
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• pp.26-33
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• 2002

In order to assess the effect of feeding raw or processed cotton (Gossypium) seed meal (CSM) on meat and wool production, 30 male crossbred lambs (3-4 months) of uniform body weight were assigned equally to five dietary treatments in a completely randomised design. The CSM was processed by three different methods i.e., cooking the meal at $100^{\circ}C$ for 45 minutes, treatment with 1% calcium hydroxide ($Ca(OH)_2$) for 24 h and iron treatment in the ratio of 1 part free gossypol (FG) to 0.3 parts of iron for 30 minutes. The lambs were fed isonitrogenous and isocaloric concentrate mixtures, containing 30% deoiled peanut meal (reference diet) and 40% of either raw, cooked, $Ca(OH)_2$ or iron treated CSM for 180 days. The raw and variously processed CSM replaced about 50% nitrogen of reference concentrate mixture. The concentrate mixtures were fed to meet 80% of the protein requirements (NRC, 1985) along with ad libitum chopped maize (Zea mays) hay. The slaughter weight, empty body weight and carcass weight was higher ($p{\leq}0.01$) in lambs fed cooked CSM incorporated diets, compared to diets containing deoiled peanut meal (DPNM). These parameters were not influenced by feeding diets containing either raw, $Ca(OH)_2$ or iron treated CSM in comparison DPNM diets. The carcass length, loin eye area and edible and inedible portion of carcass and the meat: bone ratio in whole carcass were also not affected by feeding CSM based diets. Among various primal cuts, the yield of legs was lower ($p{\leq}0.05$) from raw CSM fed lambs in comparison to DPNM fed lambs. The fat content in the Longissimus dorsi muscle was reduced ($p{\leq}0.05$) in lambs fed processed CSM based diets compared to those fed DPNM diet. Replacing DPNM with either raw or processed CSM based diets did not influence the sensory attributes and overall acceptability of meat. The wool yield was higher ($p{\leq}0.05$) in iron treated CSM fed lambs. The fibre length and fibre diameter were comparable among lambs on various dietary regimes. Among lambs fed variously processed CSM diets, the feed cost per kg of edible meat production was lower ($p{\leq}0.05$) on $Ca(OH)_2$ treated CSM, followed by cooked CSM diet and then on raw CSM based diets compared to DPNM diet. The CSM after 1% $Ca(OH)_2$ treatment or cooking for 45 minutes appears to be a satisfactory protein supplement in lamb diets for meat and wool production to replace at least 50% nitrogen of scarce and costly peanut meal.