• 한국초지조사료학회지
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• 제37권3호
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• pp.242-247
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• 2017

Humic acid (HA) is a complex organic matter found in the environments, especially in grassland soils with a high density. The bioactivity of HA to promote plant growth depends largely on its extraction sources. The quality-control of HA and the quality improvements via an artificial synthesis are thus challenging. We recently reported that a polymeric product from fungal laccase-mediated oxidation of catechol and vanillic acid (CAVA) displays a HA-like activity to enhance seed germination and salt stress tolerance in a model plant, Arabidopsis. Here, we examined whether HA or CAVA enhances the growth of Italian ryegrass seedling. Height and fresh weight of the plant with foliar application of HA or CAVA were bigger than those with only water. Interestingly, enhanced root developments were also observed in spite of the foliar treatments of HA or CAVA. Finally, we proved that HA or CAVA promotes the regrowth of Italian ryegrass after cutting. Collectively, CAVA acts as a HA mimic in Italian ryegrass cultivation, and both as a biostimulant enhanced the early growth and regrowth after cutting of Italian ryegrass, which could improve the productivity of forage crops.

• 식품보건융합연구
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• 제6권2호
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• pp.17-21
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• 2020

The response of honeybee (Apis mellifera L.) pollination on canola yield with reference to most suitable number of bee hive need per unit area of crops in order to meet optimum pollination needs and better economic yields by comparing number of hives and yield components an experiment was conducted at Beekeeping and Hill Fruit Pests Research, Station Rawalpindi during 2017-18 in complete randomized block design with two sets of four treatments for comparison: 1 hive acre^-1, 2 hives acre^-1, 3 hives acre^-1 and 0 hive acre^-1. The hives were kept inside the experimental area. Parameters were assessed: pollination density, pollinator's diversity, agronomic and economic yield. In case of pollination density, the cumulative mean abundance bee species revealed that at 1200 hours, Apis mellifera was the most abundant and frequent visitor with a mean population of 8.69 bees/plant followed by A. dorsata (0.72), Syrphid fly (0.2) and other pollinators. Minimum bee population was observed during 1400 hours, mainly due to the closure of flowers and partially due to high temperature (>35℃). Pollinator diversity revealed that A. mellifera was the most dominant pollinator of Brassica crop with highest abundance (71%). A. dosata ranked 2^nd (16%) followed by A. florea (6%) respectively.

• 한국자원식물학회지
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• 제11권3호
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• pp.290-295
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• 1998

Presowing treatements to seed are reasonable for elevating the seedling production efficiency. This experiment was carried out to determine the effects of $GA_3$ [0 (water) : 0.01 mM] and light qulaity (red : white) simultaneously treated before sowing on seedling emergence and growth of tobacco (Nicotiana tabacum L.) cv NC 82 and Burley 21. Red or white light was illuminated 12 hours a day while seeds of the cultivars were imbibed with either water or $GA_3$ 0.01 mM for 3 days. Seedling emergence was measured up to 20 days after sowing but morphological characters and dry weights were done 40 days. The seedling emergence rate of cv NC 82 was more affected by light quality and $GA_3$ pretreatments than that of cv Burley 21. Light quality more influenced the rate than $GA_3$ while red light or water imbibition enhanced the rates of the two cultivars compared to the other treatement. Although mean plant height and leaf number per seedling were nearly equal between the treatements, red light declined leaf area per seedling of cv Burley 21 of which seeds were imbibed in water but white light decreased the areas of both cultiars of which seeds were soaked in $GA_3$solution. Shoot dry weight was greater in cv NC 82 but less in cv Burley 21 pretreated with red light, and root dry weight showed the reverse result in white light, meaning that presowing light and $GA_3$ treatments could be changed the seedling development and growth of tobacco.

• Journal of Applied Biological Chemistry
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• 제62권3호
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• pp.247-250
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• 2019

Wheat is one of the most important grains. Its consumption is increasing globally. Many countries are making efforts to increase the extent of wheat harvest. It is known that plant growth promoting rhizobacteria (PGPRs) have beneficial effects on various plants. Two PGPRs including Paenibacillus pabuli strain P7S (PP7S) and Pseudomonas nitroreducens strain IHB (PnIHB) were employed to investigate effects of PGPRs on early growth of three wheat cultivars (Koso, Seakumkang, and Jokyung). While PP7S had adverse effects on Seakumkang and Jokyung, PP7S had positive effects on Koso except root length compared to control group having no treatment of PP7S. However, all treatments with PnIHB had adverse effects on germination rate, root/shoot lengths, vigor index, and dry root/shoot weights of all three wheat cultivars. These positive effects with PP7S on Koso might be related to the earlier emergence of wheat seed above soil which is known to be an indicator of increased yield. Results of the present study suggest that if proper PGPR strains are selected, they could have positive effects on early growth rate of a wheat cultivar.

• 농업과학연구
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• 제48권4호
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• pp.1003-1013
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• 2021

Thioredoxin (TRX) protein is an antioxidant responsible for reducing other proteins by exchanging cysteine thiol-disulfide and is also known for its anti-allergic and anti-aging properties. On the other hand, epidermal growth factor (EGF) is an important material used in the cosmetics industry and an essential protein necessary for dermal wound healing facilitated by the proliferation and migration of keratinocytes. EGF also assists in the formation of granulation tissues and stimulates the motility of fibroblasts. Hence, genetically modified soybeans were developed to overexpress these industrially important proteins for mass production. A single-dose oral-toxicity-based study was conducted to evaluate the potential toxic effects of TRX and EGF proteins, as safety assessments are necessary for the commercial use of seed-specific protein-expressing transgenic soybeans. To achieve this rationale, TRX and EGF proteins were mass purified from recombinant E. coli. The single-dose oral-toxicity tests of the TRX and EGF proteins were carried out in six-week old male and female Institute of Cancer Research (ICR) mice. The initial evaluation of the single-dose TRF and EGF treatments was based on monitoring the toxicity signatures and mortality rates among the mice, and the resultant mortality rates did not show any specific clinical symptoms related to the proteins. Furthermore, no significant differences were observed in the weights between the treatment and control groups of male and female ICR mice. After 14 days of treatment, no differences were observed in the autopsy reports between the various treatment and control groups. These results suggest that the minimum lethal dose of TRX and EGF proteins is higher than the allowed 2,000 mg·kg^-1 limit.

• 현장농수산연구지
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• 제18권1호
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• pp.93-100
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• 2016

벼 담수 및 무논직파재배에서 잡초성 벼(weedy rice) 및 잡초(weeds)방제를 위하여 철분 코팅볍씨를 이용하여 발아 전 제초제(pre-emerged herbicides)를 처리하여 안전한 제초제 선발 시험결과를 요약하면 다음과 같다. ① 출아일수는 제초제별로는 벤조비사이클론 >옥사디아존 > 티오벤카브 > 뷰타클로르 순으로, 사용종자별로는 철분코팅 밀봉종자(최아)> 최아종자>침종종자 철분코팅 순으로 출아가 늦었다. ② 사용종자 중 무처리와 벤조비사이클론 처리에서 침종종자 철분코팅의 입모율은 각각 63.7%, 75.7%로 최아종자 및 철분코팅 밀봉종자(최아)에 비하여 23.6~45.2%, 잡초성 벼보다는 46~62.5%나 높았다. ③ 뷰타클로르 제초제의 고사율은 최아볍씨와 철분코팅 밀봉종자(최아)에서는 100%의 고사율을 보였지만 벤조비사이클론은 2%의 고사율을 보여 비교적 안전한 것으로 나타났다. ④ 벤조비사이클론 3.5% 유제 처리의 파종후 25일 초장은 9.2~12.9cm로 무처리 11.9~16.3cm에 비하여 약간 짧았지만 정상적인 생육을 하였다.

• Journal of Radiation Protection and Research
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• 제49권3호
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• pp.102-113
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• 2024

Diffusing alpha-emitters radiation therapy (DaRT) represents a groundbreaking development in cancer therapy, offering a solution to the limitations of conventional radiation therapy. By deploying ²²⁴Ra embedded seeds, DaRT achieves targeted delivery of high-dose alpha particles directly to tumor sites, showing considerable efficacy in tumor control and minimal damage to adjacent healthy tissues. This comprehensive review analyzes the published literature regarding mechanisms, seed production, dose calculation, measurement, and biological experiments related to DaRT. It includes in-depth discussions on mathematical models, Monte Carlo simulations for dose distribution, real-time in vivo dosimetry developments, and biological experiments both in vitro and in vivo. Clinical trial outcomes are also examined to evaluate the therapy's effectiveness in various cancer types. DaRT utilizes ²²⁴Ra-labeled seeds, using the decay chain of ²²⁴Ra to deliver alpha particles effectively within a tumor. Several asymptotic diffusion-leakage models were developed to calculate the alpha dose distribution of DaRT. In vivo dosimetry techniques have been developed for real-time monitoring. Biological experiments demonstrated the cytotoxic effects of DaRT across various cancer cells, with varying radiosensitivity. Additionally, the enhanced effects of combined therapy with chemotherapy and immunotherapy were suggested by many in vivo studies. Clinical trials have shown high complete response rate in squamous cell carcinoma, with minimal side effects, suggesting DaRT's feasibility and safety. DaRT emerges as a highly localized cancer treatment method with minimal side effects compared to traditional radiation therapy. It directly ablates tumors and potentially enhances immune responses, indicating a significant advance in cancer therapy. Future research and ongoing clinical trials will further elucidate its efficacy across different cancer types and in combination with other treatments.

• 한국토양비료학회지
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• 제32권1호
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• pp.22-30
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• 1999

본 연구는 사일리지용 옥수수(Zea mays L.)에 액상분뇨를 축종과 N수준을 달리하여 시용했을 때 사일리지용 옥수수의 사초수량, 무기물함량 N균형, 토양화학성분 및 토양침투수중 질산태질소함량 변화를 구명하고자 7처리 (화학비료표준구 $200kg\;N;ha^{-1}$, 소액상분뇨구 200, 320, $440kg\;N;ha^{-1}$, 돼지액상분뇨구 200, 320, $440kg\;N;ha^{-1}$를 난괴법 3반복으로 배치하여 1997년 5월부터 1997년 8월까지 수원의 축산기술연구소 사료포장에서 수행하였으며, 그 결과를 요약하면 다음과 같다. 사일리지용 옥수수의 평균 건물률은 액상분뇨 시용처리에 따른 차이가 없었으나, 건물수량에 있어서는 유의적인 차이가 있었다(P<0.05). 또한 평균 건물수량은 돼지 액상분뇨구가 소액상분뇨구보다 높은 경향이었다. 옥수수의 무기물함량중 칼륨, 칼슘, 마그네슘은 가축액상분뇨의 시용량이 증가됨에 따라 증가하였다(P<0.05). 사일리지용 옥수수의 질산태질소함량은 소액상분뇨구에서 시용수준에 의한 영향을 밖지 않았으나 돼지액상분뇨구에서는 시용수준이 증가됨에 따라 증가하였다. 액상분뇨의 시용은 시험후 토양의 pH. 치환성양이온함량을 증가시켰다. 표토(0-10cm)중 유효인산함량은 돼지 액상분뇨 $440kg\;N;ha^{-1}$구에서 $340mg\;kg^{-1}$으로 가장 높았다. 토양침투수중 질산태질소 농도는 돼지액상분뇨 $440kg\;N;ha^{-1}$구에서 증가하였다. 가축액상분뇨 시용량이 많을수록 N 수지량은 증가되었다. 이상의 연구결과를 볼 때 소액상분뇨구 보다 돼지액상분뇨구의 평균건물수량이 높았고, N 수지량은 시용수준의 증가에 따라 높아졌다.

• 농업과학연구
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• 제20권2호
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• pp.125-132
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• 1993

형태적(形態的)으로 서로 다른 두 교잡종(交雜種) 옥수수의 물질(物質) 생산성(生産性) 형질(形質)(source)에 대한 저장(貯藏) 수용능력(受容能力)(sink)효과를 비교하고자 대조구(對照區)인 무처리(無處理)를 포함하여 전체엽(全體葉) 제거구, 첫째이삭 절위(節位)의 상위엽(上位葉) 제거구, 첫째이삭 절위(節位)의 하위엽(下位葉) 제거구, 첫째이삭만 제거구등 5개 처리에 대한 생육특성(生育特性) 및 종실중(種實重)에 미치는 효과를 요약하면 다음과 같다. 1. 간장(肝腸)은 두 공시 교잡종(交雜種) 모두 무처리(無處理)에서 가장 컸으나 기타 처리간에는 큰 차이가 없었다. 2. 엽중(葉重)은 상위엽제거구(上位葉除去區)와 하위엽제거구(下位葉除去區)에서 분얼형(分蘖型)이나 무분얼형(無分蘖型) 모두 같은 수준이었으나 교잡종간에는 분얼형(分蘖型)의 엽중(葉重)이 무분얼형(無分蘖型)보다 5-10g정도가 많았으며, 줄기무게 엽중(葉重)과 비슷한 경향이었다. 4. 공시종 모두가 입중(粒重)은 무처리에 비해 낮았는데 특히 무처리에 비해 상위엽(上位葉) 제거구가 60%정도 낮았고 하위엽(下位葉)제거구는 40%정도가 낮았는데 분얼형(分蘖型) 보다는 무분얼형(無分蘖型)의 옥수수에서 입중(粒重)의 감소가 켰다. 5. 분산분석(分散分析) 결과 줄기중(重)과 입중(粒重)은 교장종간에 큰 차이를 보였고, 처리간(處理間), 교잡종(交雜種)과 처리간(處理間) 상호작용(相互作用)에 있어서는 조사된 모든 특성(特性)에서 유의성(有意性)이 인정 되었다. 6. 전체(全體) 건물중(乾物重)에 대한 각 부위별(部位別) 기여도(寄與度)를 보면 무처리에서는 입중(粒重), 엽중(葉重), 줄기중(重) 순(順)으로 나타났고 상위엽(上位葉)제거구는 줄기중(重), 엽중(葉重), 입중(粒重) 순(順)이었는데 비해 하위엽(下位葉)제거구는 입중(粒重)의 기여(寄與)가 매우 컸으며 다음은 엽중(葉重)과 줄기무게가 같은 수준으로 나타났다.

• 생물환경조절학회지
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• 제28권2호
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• pp.110-116
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• 2019

상토에 기비로 혼합된 질소의 $NH_4{^+}:NO_3{^-}$ 비율이 '녹광' 고추의 생장에 미치는 영향을 구명하고자 본 연구를 수행하였다. 실험을 위해 코이어 더스트, 피트모스 및 펄라이트를 부피 기준 35:35:30%로 혼합한 상토를 조제하였으며, 상토원료 혼합과정에서 총 질소 농도가 $300mg{\cdot}L^{-1}$인 조건에서 $NH_4{^+}:NO_3{^-}$ 비율을 0:100, 27:73, 50:50, 73:27 및 100:0으로 조절한 5처리를 두어 실험하였다. 질소 외에 다른 필수원소를 포함한 비료는 모든 처리에서 동일한 농도로 조절하였고, 비료를 포함한 상토를 50구 플러그 트레이에 충진한 뒤 고추 종자를 파종하였다. 파종 전과 파종 후 상토의 pH, EC 및 다량원소 농도를 분석하였으며, 파종 7주 후에는 지상부 생장조사와 식물체 무기원소 함량을 분석하였다. 파종 전 상토 pH는 질소 비율에 따른 차이가 뚜렷하지 않았으나 생장이 진행되면서 $NO_3{^-}$의 비율이 높은 처리의 상토 pH가 상승하였다. $NH_4{^+}$의 비율이 클수록 파종 전 상토의 EC가 높았으나 모든 처리의 EC가 파종 3주 후부터 급격히 낮아지는 경향을 보였다. 고추묘 생장이 진행됨에 따라 상토의 $NH_4{^+}$-N, $NO_3{^-}$-N 그리고 다른 종류의 다량원소 농도가 EC 변화와 유사한 경향을 보이며 낮아졌다. 파종 7주 후 고추의 지상부 생장을 조사한 결과 $NH_4{^+}:NO_3{^-}$ 비율이 27:73과 50:50 처리구에서 전반적인 생장이 우수하였고, $NH_4{^+}$ 비율이 73% 이상일 경우 저조한 생장을 보였다. 또한 생장이 가장 우수하였던 50:50($NH_4{^+}:NO_3{^-}$) 처리구의 식물체내 T-N, K, Ca 및 Mg 등 다량 무기원소 함량이 가장 높았다. 이와 같은 결과를 고려할 때 '녹광' 고추의 플러그 묘 생장을 위해서는 상토에 기비로 혼합하는 전체 질소 중 $NH_4{^+}$의 비율을 27% 또는 50%로 조절하는 것이 묘 생장에 유리할 것으로 판단하였다.