• Title/Summary/Keyword: Seed addition method

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Mass Propagation of Sundew, Drosera rotundifolia L. through Shoot Culture

  • Jang, Gi-Won;Park, Ro-Dong
    • Journal of Plant Biotechnology
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    • v.1 no.2
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    • pp.97-100
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    • 1999
  • In order to establish in vitro propagation method of sundew, Drosera rotundifolia L., the effects of MS medium concentration, cytokinin type and concentration, pH, and auxin type and concentration on shoot proliferation and root formation were investigated using shoots at 3 month after seed germination. The highest shoot production was obtained with the half strength of MS ($\frac{1}{2}$ MS) medium than with any other strength of MS medium tested. Addition of kinetin or BA in $\frac{1}{2}$ MS medium was strongly suppressed shoot proliferation. The suppression of shoot proliferation was more effective in BA-supplemented $\frac{1}{2}$ MS medium than kinetin-supplemented. The optimum pH of the media for shoot proliferation was pH 5.7-6.7. Shoots were subcultured in $\frac{1}{2}$ MS medium supplemented with 0.5mg/L 2,4-D for rooting every 8 weeks. All subcultured shoots produced extensive root systems after 5 to 6 week culture. Plantlets after root development were planted in plastic pots filled with moss. The survival rate of plantlets was almost 100%. On subculturing every 8 weeks, hundreds of the plants were propagated from a single plant within a year.

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In Vitro and Greenhouse Evaluation of Cucumber Growth Enhanced by Rhizosphere Microorganisms (실험실내와 비닐하우스에서 근권 미생물에 의한 오이 생육증진의 검정)

  • 배영석;장성식;박창석;김희규
    • Korean Journal Plant Pathology
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    • v.11 no.4
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    • pp.292-297
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    • 1995
  • We developed an in vitro assay method for evaluating plant growth promotion and providing an evidence that the growth promotion is rendered by growth enhancing factors. The amendment of culture filtrates of Trichoderma harzianum T95 and Gliocladium virens G872 and G872B in Murashige and Skoog (MS) agar medium enhanced the cotyledon growth of cucumber in terms of fresh weight and primary leaf area of cucumber cotyledon cuttings, of which the treatment of G. virens G872B was the most effective. The mycelial culture filtrate of G872B was more effective in the growth promotion than its conidial germling filtrate. The addition of 1% sucrose in MS mineral medium with 0.1% culture filtrates of the antagonists (T95 and G872B) was optimum for enhancing the effect of the filtrates on the growth of cotyledon cuttings in vitro. When cucumber seeds treated with G872B, Pseudomonas putida Pf3 or the G872B-Pf3 mixture were planted in a greenhouse, the rate of seed germination, biomass of shoot and root, and yield of cucumber fruits were increased, especially by G872B or the G872B-Pf3 mixture. Correspondingly, cucumber fruit yields in early to middle-cycles of harvest were significantly greater in the plots of G872B than the control and Pf3-treated plots, and the final yield was highest in the plots of the G872B-Pf3 mixture applications.

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Development of a Vegetable Transplanting Robot

  • Kang, Dong-Hyeon;Kim, Dong-Eok;Lee, Gong-In;Kim, You-Ho;Lee, Hye-Jin;Min, Young-Bong
    • Journal of Biosystems Engineering
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    • v.37 no.3
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    • pp.201-208
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    • 2012
  • Purpose: This study was conducted to develop a vegetable transplanting robot which transplants seedlings from a nursery tray to a plant pot using its four fingers. Method: The robot consists of the transplanting part, nursery tray moving part, plant pot moving part, main frame and controller. Two moving parts are controlled by the photo sensor and servo motor. The transplanting part was composed with two components; transporting device using E-MY2H(SMC Corp., Japan) for controlling finger position accurately and finger for transplanting the seedling. Results: Head lettuce using the transplanting robot was transplanted in 21 days after sowing based upon the seed shape measurement and removing examination. The optimal finger shape was thin pin type because it caused minimum damage to the roots of seedlings. Conclusions: The four inclined pin type was applied to remove the seedlings from the nursery tray. In addition, the transplanting capacity of the developed robot was 2800 pots per hour and the rate of success was 99% and above.

Enhancing Resistance of Red Pepper to Phytophthora Blight Diseases by Seed Treatment with Plant Growth Promoting Rhizobacteria

  • M. Rajkumar;Lee, Kui-Jae;Park, Min-Kyung;Jo, Rae-Yun;Lee, Wang-Hui
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2003.10b
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    • pp.47-47
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    • 2003
  • Plant growth promoting rhizobacteria (PGPR) have been shown to suppress phytopthora blight. This suppression has been related to both microbial antagonism and induced resistance. The PGPR isolates were screened by dual culture plate method and most of the isolates were showed varyinglevels of antagonism. Among the PGPR isolates pyoverdin, pyochelin and salicylic acid producing strains showed the maximum inhibition of mycelial growth of Phytophthora capsici and increased plant growth promotion in red pepper. PGPR isolatesfurther analysed for its ability to induce production of defence related enzymes and chemicals. The activities such as Phenyle alanin ammonia lyase (PAL), Peroxidase (PO), Polyphenol oxidase (PPO) and accumulation of phenolics were observed in PGPR pretreated red pepper plants challenged with Phytophthora capsici. The present study shows that an addition of direct antagonism and plant growth promotion, induction of defense related enzymes involved to enhance resistance against invasion of P. capsici in red pepper.

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Comparison of marine environment and oyster culture between in Gamak and Hiroshima Bays (가막만과 히로시마만의 해양환경과 굴양식의 비교)

  • Lee, Moon-Ock;Kim, Jong-Kyu;Kim, Byeong-Kuk;Kwon, Yeong-Ah
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2006.11a
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    • pp.481-484
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    • 2006
  • This research compares oyster farming in Gamak Bay which Hiroshima Bay which has nearly similar marine environments. The marine environment of these two waters has similar variations of air temperature, sea surface temperature, precipitation and salinity. However Hiroshima Bay is higher than Gamak Bay in the sea surface temperature and Hiroshima Bay is also higher than Gamak Bay in the salinity. Their oyster farming method is basically similar but it is different in their facilities. We need to take some measures against high mortalities, and in addition, to enhance the rate of seed collection.

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A Novel Classification Model for Efficient Patent Information Research (효율적인 특허정보 조사를 위한 분류 모형)

  • Kim, Youngho;Park, Sangsung;Jang, Dongsik
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.15 no.4
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    • pp.103-110
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    • 2019
  • A patent contains detailed information of the developed technology and is published to the public. Thus, patents can be used to overcome the limitations of traditional technology trend research and prediction techniques. Recently, due to the advantages of patented analytical methodology, IP R&D is carried out worldwide. The patent is big data and has a huge amount, various domains, and structured and unstructured data characteristics. For this reason, there are many difficulties in collecting and researching patent information. Patent research generally writes the Search formula to collect patent documents from DB. The collected patent documents contain some noise patents that are irrelevant to the purpose of analysis, so they are removed. However, eliminating noise patents is a manual task of reading and classifying technology, which is time consuming and expensive. In this study, we propose a model that automatically classifies The Noise patent for efficient patent information research. The proposed method performs Patent Embedding using Word2Vec and generates Noise seed label. In addition, noise patent classification is performed using the Random forest. The experimental data is published and registered with the USPTO among the patents related to Ocean Surveillance & Tracking Network technology. As a result of experimenting with the proposed model, it showed 73% accuracy with the label actually given by experts.

Synthesis of Nano Size $BaCeO_3$ as an Effective Flux Pining Center for YBCO Superconductor (YBCO 초전도체의 효과적인 플럭스 피닝 센터로서의 나노 크기 $BaCeO_3$ 합성)

  • Youn, J.S.;No, K.S.;Kim, Y.H.;Jun, B.H.;Lee, J.P.;Jung, S.Y.;Kim, C.J.
    • Progress in Superconductivity
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    • v.10 no.1
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    • pp.12-16
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    • 2008
  • In this work, nano size $BaCeO_3$, which is a possible flux pinning medium of melt processed $YBa_{2}Cu_{3}O_x$ superconductor, was synthesized by the conventional solid state reaction method using powders. $BaCeO_3$ and $CeO_2$ were mixed thoroughly using a ball milling for 24 hours and calcined at $1200^{\circ}C$ for 5 hours for the formation $BaCeO_3$ powder. The obtained $BaCeO_3$ powder was attrition milled at various milling times of 60 min, 120 min and 240 min. The $BaCeO_3$ powders of various milling times were mixed with $YBa_{2}Cu_{3}O_x$ powder. Seed melt processed $YBa_{2}Cu_{3}O_x$-$BaCeO_3$ (15wt.%) superconductors were prepared and the superconducting properties were investigated. It was found that $T_c$ of $Y_{1.5}Ba_{2}Cu_{3}O_x$ samples was not significantly affected by $BaCeO_3$ addition, but $J_c$ of samples was increased by $BaCeO_3$ addition. The $J_c$ improvement by fine $BaCeO_3$ powder (120 min attrition-milled) was effective at low magnetic fields less than 2 T.

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Market Segmentation Based on Types of Motivations to Visit Coffee Shops (커피전문점 방문동기유형에 따른 시장세분화)

  • Lee, Yong-Sook;Kim, Eun-Jung;Park, Heung-Jin
    • The Korean Journal of Franchise Management
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    • v.7 no.1
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    • pp.21-29
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    • 2016
  • Purpose - The primary purpose of this study is to employ effective marketing methods using market segmentation of coffee shops by determining how motivations to visit coffee shops have different impacts on demographic profile of visitors and characteristics of coffee shop visits, so as to draw out a better understanding of customers of coffee market. Research design, data, and methodology - Data were collected using surveys of self-administered questionnaires toward coffee shop users in Daejeon, Korea. A number of samples used in data analysis were 253 excluding unusable responses. The data were analyzed through frequency, reliability, and factor analysis using SPSS 20.0. Factor analysis was conducted through the principal component analysis and varimax rotation method to derive factors of one or more eigen values. In addition, the cluster analysis, multivariate ANOVA, and cross-tab analysis were used for the market segmentation based on the types of motivation for coffee shop visits. The process of the cluster analysis is as follows. Four clusters were derived through hierarchical clustering, and k-means cluster analysis was then carried out using mean value of the four clusters as the initial seed value. Result - The factor analysis delineated four dimensions of motivation to visit coffee shops: ostentation motivation, hedonic motivation, esthetic motivation, utility motivation. The cluster analysis yielded four clusters: utility and esthetic seekers, hedonic seekers, utility seekers, ostentation seekers. In order to further specify the profile of four clusters, each cluster was cross tabulated with socio-demographics and characteristics of coffee shop visits. Four clusters are significantly different from each other by four types of motivations for coffee shop visits. Conclusions - This study has empirically examined the difference in demographic profile of visitors and characteristics of coffee shop visits by motivation to visit coffee shops. There are significant differences according to age, education background, marital status, occupation and monthly income. In addition, coffee shops use pattern characterization in frequency of visits to coffee shops, relationships with companion, purpose of visit, information sources, brand type, average expense per visit, important elements of selection attribute were significantly different depending on motivations for coffee shop visits.

A Study of Modified Parallel Feistel Structure of Data Speed-up DES (DES의 데이터 처리속도 향상을 위한 변형된 병렬 Feistel 구조에 관한 연구)

  • Lee, Seon-Keun;kIM, Hyeoung-Kyun;Kim, Hwan-Yong
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.37 no.12
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    • pp.91-97
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    • 2000
  • With the brilliant development of information communication and the rapid spread of internet, current network communication is carrying several up-to-date functions such as electronic commerce, activation of electro currency or electronic signature and will produce more advanced services in the future. Information communication network such as that electronic commerce would demand the more safe and transparent guard of network, and anticipate the more fast performance of network. In this paper, in order to meet the several demands, DES(data encryption standard) with parallel feistel structure, which feistel structure of the basic structure of DES is transformed into in parallel, is proposed. The existing feistel structure can't use pipeline method for the structural problem of DES itself-the propagation of error. therefore, this modified parallel feistel structure could improve largely the performance of DES which had to have the trade-off relation between data processing speed and data security and in addition a method proposed in SEED having adopted the modified parallel feistel structure shows more excellent secure function and/or fast processing ability. The used CAD Tool use Synopsys Ver. 1999. 10 in both of synthesis and simulation.

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Exploration of Optimum Retention of Antibacterial Agents in Functional Packaging Paper (항균 포장원지내 항균소재의 최적 정착법 탐색)

  • Kim, Chul-Hwan;Kim, Jae-Ok;Jung, Jun-Ho;Cho, Sung-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.2
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    • pp.298-305
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    • 2005
  • Antimicrobial packaging paper was prepared with a powder-type botanical antimicrobial agent from grapefruit seed extract (BAAG) and zeolite according to TAPPI standard method. The functional fillers containing BAAG fixed to CaCO$_3$ and zeolite were well retained in the fiber network by a retention aid such as cationic polyacrylamide, which was ascertained by the ash contents of paper and the SEM microphotographs. With addition of the functional fillers to paper, tensile strength and burst strength of the paper decreased by interference of the functional fillers with interfiber bonding but bending stiffness and tear strength increased by improved elastic modulus of paper and delayed transfer of tearing energy. Finally, it was confirmed that the antimicrobial packaging paper might be able to be used to make packaging bags and corrugated containers due to the minor deterioration of strength properties.