• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.2
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• pp.106-112
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• 2012

In order to understand the growth and ecophy -siological response of ginseng to global warming condition, we cultivated one and two year ginseng seedlings in control (ambient $CO_2$ + ambient temperature) and global warming treatment (elevated $CO_2$ + elevated temperature) from March 2010 to July 2011. Shoot appearance and initiation of flowering were advanced by 3-4 days in global warming treatment than in control. However, timing of fruit setting and seed ripeness was similar in both control and global warming treatment. Shoot length was longer in global warming treatment than in control, and also the number of leaves was much in global warming treatment. Fresh root weight was not different between control and global warming treatment. Photosynthetic rate was higher in global warming treatment than at control. Photosynthetic rate and transpiration rate were higher in two year seedlings than in one year seedlings at control, but was not different between seedling age of ginseng in global warming treatment. Water use efficiency was higher in one year seedlings than two year seedlings at control and global warming treatment. These results demonstrated that Korean ginseng more or less positively responds to global warming situation.

• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.4
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• pp.661-667
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• 2010

Astragali Radix (AR) has been used in traditional medicinal herb, which is known to tonify the qi and blood. It has been asserted to be a tonic that can improve the function of the lungs, adrenal glands and the gastrointestinal tract, increase metabolism, promote healing and reduce fatigue. Also, Plantago asiatica (PA) has the anti-inflammatory, antiseptic, antitussive, cardiac, diuretic, expectorant, haemostatic effects, though it is considered to be less powerful than the seed. Recent research is showing that it is promising in lowering cholesterol and controlling diabetes. Obesity is characterized at the cellular level by an increase in the number and size of adipocytes differentiated from fibroblastic preadipocytes in adipose tissue. In the present study, we investigated the effects of 3 to 7 extracts of mixed medicinal herbs (water and ethanol extract of Astragali Radix; ARW and ARE, water and ethanol extract of Plantago asitica; PAW and PAE, and those mixed extracts; ARW+PAE, ARW+PAW and ARE+PAE) on adipogenesis and apoptosis in 3T3-L1 adipocytes by MTT assay, Oil-Red-O staining and TUNEL assay. And then, we determined total contents of phenolic compounds and flavonoid compounds in mixed herbal extracts. These result showed that herbal extracts (ARW, PAE and ARW+PAE) affected on adipogenesis and apoptosis in 3T3-L1 adipocytes among 3 to 7 extracts tested. Especially herbal mixed extracts (ARW+PAE) had the highest inhibition on differentiation of preadipocytes and lipoid accumulation of maturing adipocytes. And it did not affect cell viability of mature adipocytes, but herbal mixed extracts (ARW+PAE) increased apoptosis in mature adipocytes, as proved by highest concentration $200\;{\mu}g/m{\ell}$ using TUNEL assay. In addition, contents of total phenoilc compounds and flavonoids showed the highest level in ARE+PAE and PAE than ARW. These results suggest that this main target for adipocyte functions could be effective in improving the metabolic syndrome including obesity.

• Journal of Plant Biotechnology
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• v.39 no.4
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• pp.235-241
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• 2012

Sumoylation is a reversible conjugation process that attaches the small ubiquitin modifier (SUMO) peptide to target proteins and regulates a wide variety of cellular functions in eucaryotes. As final step of the sumoylation, SUMO E3 ligases facilitate conjugation of SUMO to target proteins. To characterize the functions of the SUMO E3 ligases in Oryza sativa, we isolated a single recessive rice SUMO E3 ligase, Ossiz1-2 mutant. In addition, we also confirmed the interaction between OsSIZ1/-2 and OsSUMO1, respectively, by using an Agrobacterium-based tobacco luciferase transient expression system. Ossiz1-2 mutant exhibited approximately 20% reduction in growth and developmental units compared with wild type. Especially, number of filled seeds and total seed weight were dramatically decreased in the Ossiz1-2 mutant rice. Thus, these results suggest that sumoylation by the OsSIZ1 as SUMO E3 ligase plays an important role in regulating growth and development in rice.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.3
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• pp.205-210
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• 1994

This experiment was conducted to investigate of soil hardening degree before sowing, furrow depth at sowing and flooding time after sowing at furrowing in flooded rice paddy field that many people have an interest in as direct sowing method most recently. As hardening period was increased, the percentage of seedling stand and seed floating at flooding were increased slightly, while buried depth of stem at maximum tillering stage and cone penetration depth were decreased, respectively. Therefore, optimum degree of soil hardening was about 3 days after draining, at this time, cone penetration degree was about 6~7cm. According to furrow depth was more and more deep, buried depth of stem was increased gradually, but percentage of seedling stand was decreased considerbly. Also, root distribution ratio on surpace horizon and lodging degree were increased gradually according to furrow depth become more and more shallow. As flooding time after sowing was late, percentage of seedling stand and panicle number per $m^2$ were decreased slightly. These results apparently indicated that sowing after 3 days hardening when cone penetration degree was 6~7cm, furrow depth 3~4cm degree and flooding time just after sowing the best method to good establish of seedling stand.

• Journal of Plant Biotechnology
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• v.38 no.2
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• pp.105-116
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• 2011

Transgenic zoysiagrass (Zoysia japonica Steud.) expressing the bar gene inserted in the plant genome has been generated previously through Agrobacterium tumefaciens-mediated transformation. The GM zoysiagrass (event: JG21) permits efficient management of weed control of widely cultivated zoysiagrass fields, reducing the frequency and cost of using various herbicides for weed control. Now we have carried out the environmental risk assessment of JG21 prior to applying to the governmental regulatory agency for the commercial release of the GM turf grass outside of test plots. The morphological phenotypes, molecular analysis, weediness and gene flow from each test plot of JG21 and wild-type zoysiagrasses have been evaluated by selectively analyzing environmental effects. There were no marked differences in morphological phenotypes between JG21 and wild-type grasses. The JG21 retained its stable integration in the host plant in T1 generation, exhibiting a 3:1 segregation ratio according to the Mendelian genetics. We confirmed the copy number (1) of JG21 by using Southern blot analysis, as the transgenic plants were tolerant to ammonium glufosinate throughout the culture period. From cross-fertilization and gene flow studies, we found a 9% cross-pollination rate at the center of JG21 field and 0% at distances over 3 m from the field. The JG21 and wild-type zoysiagrass plants are not considered "weed" because zoysiagrasses generally are not dominant and do not spread into weedy areas easily. We assessed the horizontal gene transfer (HGT) of the transgene DNA to soil microorganisms from JG21 and wild-type plants. The bar gene was not detected from the total genomic DNA extracted from each rhizosphere soil of GM and non-GM Zoysia grass fields. Through the monitoring of JG21 transgene's unintentional release into the environment, we found no evidence for either pollen mediated gene flow of zoysiagrass or seed dispersal from the test field within a 3 km radius of the natural habitat.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.3
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• pp.291-297
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• 1984

The peanut, unlike other plants, has three distinct steps in the reproductive process. These are the flowers, the peg and the fruit. This research was designed in the field to determine the difference of fruiting habit for each of plant types of peanut (Arachis hypogaea L.) in peanut culture limiting region. Eight cultivars representing four plant types, i.e., Virginia, Spanish, Valencia and Shinpung types. Number of pegs per plant produced in order of Spanish, Valencia, Virginia, Shinpung, Virginia type. The Virginia small seed type produced the most pods per plant. The shinpung type showed more percentage of matured pod than other types and early pot-set habit. Distribution of mature pods for each of branch positions to total mature pods occupied 63% on two cotyledonary branchs, 34% on 3rd to 5th branchs, 3% on over 6th branchs. Shinpung type was considered more favorable plant type than other types for breeding of early maturing high yielding variety because of early pot-set and high percentage of mature pod.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.3
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• pp.303-309
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• 1989

Present study was conducted to reveal the effects of the herbicides, Lasso and Devrinol, on the soybean growth and the nodulation in field condition. Emergence rate was reduced positively in proportion to increase in the concentration of herbicides regardless of the herbicidal difference and it was significantly reduced even in the recommended concentration as compared to untreated plot, showing marked abnormal symptom on seedlings. Plant height, fresh weight of the plant, number of internodes, branches, pods, seeds per plant and 100-seed weight were reduced with increase in the concentration of herbicides and were highly significant in difference between the untreated plot and double concentration plot, eventhough most were nonsignificant in difference from the recommended concentration. Nodulation was significantly decreased with increase in the concentration of herbicides. The reduction was remarkably different with soybean varieties and consistently appeared from three weeks to six weeks after sawing. Significant correlation was realized between the reduction of nodulation and the agronomic characters of soybean and it was considered that the reduction of nodulation by misapplication of the herbicides might be a causal factor for decrease in soybean yield.

• Korean Journal of Environmental Agriculture
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• v.17 no.1
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• pp.76-83
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• 1998

The most remarkable aspect in the hormesis law is that dose of harmful agents can produce effect that are diametrically opposite to the effect found with high doses of the same agent. Minute quantities of a harmful agent bring about very small change in the organism and control mechanisms appear to subjugate normal processes to place the organism in a state of albert and repair. The stimulated organism in more responsive to changes in environmental factors than it did before being alerted. Routine functions, including repair and defense, have priority for available energy and matetial. The alerted organism utilizes nutrients more efficiently, grows faster, shows improved defense, and lives longer. Accelerated germination, sprouting, growth, development, blooming and ripening, and increased crop yield and resistance to disease are found in plants. Another concept supported by the data in that low doses of ionizing radiation provide increased resistance to subsequent high doses of radiation. The hormesis varies with subject plant, variety, state of seed, environmental and cultural conditions, physiologic function measured, dose rate and total exposure. The results of hormesis are less consistently found, probably due to the great number of uncontrolled variables in the experiments. The general dosage for radiation homlesis in about 100 (10 to 1,000) times ambient or 100 (10 to 1,000) times less than a definitely harmful dose, but these must be modified to the occasion. Although little is known about most mechanisms of homzesis reaction, overcompensation of repair mechanism is offered as one mechanism.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.608-618
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• 1995

This study was conducted to determine physiobiochemical basis of heterosis using rice hybrids such as Shanyou 63 (Zhenshan 97As Minhui 63) and Teyou 63 (Longtepu A Minhui 63) as compared with inbred rice like Milyang 23. Seed protein patterns of rice hybrid showed complementary genetic characteristics inherited from their parents. Hybrid rice had larger embryo and higher $\alpha$-amylase activity than those of inbred rice. The larger embryo of hybrid was significantly correlated with tillering ability and high number of low node tillers jplant increased by 60~70% in Shanyou 63, leading to higher productive tillers/plant which directly influenced on grain yield of hybrid rice. These characters were further supported by high chlorophyll content in hybrids. Exogenous application of GA$_3$ (0.02 ppm) on inbred rice like Milyang 23, increased significantly $\alpha$-amylase activity, but no effect of GA$_3$ on hybrid rice was observed, indicating that sufficient amount of GA$_3$ is endogenously present in hybrid rice, showing 1 to 3.5 fold higher activity of $\alpha$-amylase in hybrid rice, which trigger heterosis from the germinating stage. Further, activity of cytochrome c oxidase was 2.66 to 5.52 fold higher in hybrid rice than that of inbred rice, indicating that rice hybrids have very active metabolism reflecting vigorous growth starting from the germinating stage, in turn leading to higher tillering ability.

• Journal of Bio-Environment Control
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• v.10 no.3
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• pp.187-196
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• 2001

Asparagus varieties popular in the foreign countries were collected and tested on their productivities and qualities for five years under rain shelter and open field conditions. Among the tested varieties, cultivars of UC309 and Excel showed superior productivity to the others in spear weight but the initial growth was not different among the tested varieties. However, the growth index was higher than the other varieties. According to the result from eight-year experiment, the best variety in productivity and quality were UC309 and Excel both for rain shelter and open field cultivations. To avoid the damage caused by stem blight, rain shelter and soil surface mulching treatments were tested with var. Excel. The spear sprouting and first harvesting date were a few days earlier in rain shelter cultivation than in open field, but there were no differences among the mulching materials. The number of spears and the spear weight per plant were increased in rain shelter cultivation, especially with rice straw and black P.E. mulching treatments. Marketable yield also increased by 253% in black P.E film mulching in rain shelter cultivation compared to non-mulching in open field. The stem blight examined in the autumn, 1997 was not observed under rain shelter treatment, but severe damage was observed in the open field regardless of the mulching materials. In the experiments, high asparagus yield could be achieved by black P.E. film mulching in rain shelter cultivation without the occurrence of stem blight.