• Plant Biotechnology Reports
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• v.3 no.2
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• pp.157-165
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• 2009

Human erythropoietin (EPO) is a leading product in the biopharmaceutical market, but functional EPO has only been produced in mammalian cells, which limits its application and drives up the production costs. Using plants to produce human proteins may be an alternative way to reduce the cost. However, a recent report demonstrated that overexpression of the human EPO gene (EPO) in tobacco or Arabidopsis rendered males sterile and retarded vegetative growth, which raises concern whether EPO might interfere with hormone levels in transgenic plants. In the present study, we demonstrated that overexpressing EPO with additional 5'-His tag and 3' ER-retention peptides in tobacco did not cause any developmental defect compared to GUS plants. With our method, all 20 transgenic plants grew on selective medium and, further confirmed by PCR, were fertile. Most of them grew similarly compared to GUS plants. Only one transgenic plant (EPO2) was shorter in plant height but had twice the life span compared to other transgenic plants. When 11 randomly selected EPO plants, along with the abnormal plant EPO2, were subjected to RT-PCR analysis, all of them had detectable EPO transcripts. However, their protein levels varied considerably; seven of them had detectable EPO proteins analyzed by western blot. Our results indicate that overexpressing human EPO protein in plants does not have detrimental effects on growth and development. Our transformation systems allow us to further explore the possibility of glycoengineering tobacco plants for producing functional EPO and its derivatives.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.25 no.2
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• pp.108-115
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• 2015

Electro-mechanical actuator installed on aircraft consists of a decelerator which magnifies the torque in order to rotate an axis connected with aircraft control surface, a control section which controls the motor assembly through receiving orders from cockpit and a motor assembly which rotates the decelerator. Electro-mechanical actuator controls aircraft altitude, position, landing, takeoff, etc. It is an important part of a aircraft. Aircraft maneuvering causes vibrations to electro-mechanical actuator. Vibrations may result in structural fatigue. For that reason, it is necessary to analyze the system structural safety. In order to analyze the system structural safety. It is needed reasonable finite element model and structural response stress closed to real value. In this paper, analytic model is derived by using the simplified finite element model, and damping ratio which is closely related to response stress is derived by using modal test. So, we developed analytic model in less than 10 % error rate, compared with modal test. Vibration response stress close to real value was estimated from analytic model modified with modal experimental damping ratio. Estimation method for damping ratio with empirical formula was suggested partly. Finally, It was proved that electro-mechanical actuator had reasonable structure margin of safety at environmental random $3{\sigma}$ stress during life cycle.

• Journal of Korea Water Resources Association
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• v.43 no.2
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• pp.233-243
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• 2010

A method is proposed of estimating Muskingum-Cunge parameters for natural streams using cross-sectional and longitudinal channel geometry and roughness coefficient data. Firstly, for various water-surface levels at a cross section cross-sectional areas and hydraulic radii are calculated. Corresponding discharges are then calculated using Manning's equation. This procedure is repeated for all cross-sections in the reach. Finally, routing parameters are estimated from the calculated cross-sectional area and discharge value pairs by regression analysis. The procedures for estimating Muskingum-Cunge parameters are applied to the South Han River. Flows calculated by Muskingum-Cunge model with estimated parameters showed much better agreement with those by dynamic wave model in peak discharge, time to peak discharge, and normalized RMS errors than those calculated by the HEC-1 Muskingum-Cunge model.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.08a
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• pp.225-225
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• 2011

The dielectric and optical properties of GaInZnO (GIZO), HfInZnO (HIZO) and InZnO (IZO) thin films on glass by RF magnetron sputtering method were investiged using reflection electron energy loss spectroscopy (REELS). The band gap was estimated from the onset values of REELS spectra. The band gaps of GIZO, HIZO and IZO thin films are 3.1 eV, 3.5 eV and 3.0 eV, respectively, Hf and Ga incorporated into IZO results in an increase in the energy band gap of IZO by 0.5 eV and 0.1 eV. The dielectric functions were determined by comparing the effective cross section determined from experimental REELS with a rigorous model calculation based on the dielectric response theory, using available software package, good agreement between the experimental and fitting results gives confidence in the accuracy of the determined dielectric function. The main peak of Energy Loss Function (ELF) obtained from IZO shows at 18.42 eV, which shifted to 19.43 eV and 18.15 eV for GIZO and HIZO respectively, because indicates the corporation of cation Ga and Hf in the composition. The optical properties represented by the dielectric function e, the refractive index n, the extinction coefficient k, and the transmission coefficient, T of HIZO and IZO thin films were determined from a quantitative analysis of REELS. The transmission coefficient was increased to 93% and decreased to 87% in the visible region with the incorporation of Hf and Ga in the IZO compound.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.4
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• pp.121-126
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• 2010

The automotive weather strip has functions of isolating of water, dust, noise and vibration from outside. To achieve good sealing performance, weather strip should be designed to have the high contact force and wide contact area. However, these design causes excessive permanent deformation of weather strip. The causes of permanent deformation is generally explained to be the chemical material detrioration and physical variation and cyclic loading, etc. This paper introduces a numerical method to predict the permanent deformation using the time dependent viscoelastic model which is represented by Prony series in ABAQUS. Uniaxial tension and creep tests were conducted to obtain the material data. And the lab. test for the permanent deformation was accelerated during shorter time, 300 hours. The permanent deformation of weather strip was successfully predicted under the different loading conditions and different section shapes using the suggested numerical process.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.03a
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• pp.308-319
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• 2009

In the case of relatively good ground and construction condition in the deep excavation for the construction of subway, railway, building etc., flexible earth retaining systems are often used in an economical point of view. It is generally known that the mechanism of behavior in the flexible earth retaining system is relatively more complicated than the rigid earth retaining system. Moreover in the case of long span strut supporting system the analysis of strut axial force change becomes more difficult when the differences of ground condition and excavation work progress on both sides of excavation section are added. When deeper excavation than the specification or installation delay of supporting system is done or change of ground condition is faced due to the construction conditions during construction process, lots of axial force can be induced in some struts and that can threaten the safety of construction. This paper introduces two examples of long span deep excavation where struts and rock bolts were used as a supporting system with flexible wall structure. And the sections of two examples are 50 meters apart in one construction site, they have almost similar design and construction conditions. The characteristics of ground deformation and strut axial force change were analysed, the similarity and difference between measurement results of tow examples were compared and investigated. The effort of this article aims to improve and develop the technique of design and construction in the coming projects having similar ground condition and supporting method.

• Journal of Korea Game Society
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• v.17 no.6
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• pp.39-50
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• 2017

Gamification in education widely used in the field of computer science (CS). The purpose of this study is to analyze the pre-studies of gamification cases in CS education and suggest the direction of CS education. For the study, an empirical research method was applied. Through keyword search, 1220 pre-studies were collected, among which 55 were compressed through the first and second extraction processes. In the results section, this study summarized the presented year, applied subject, subject of education, results and limitations. Additional study not only will analyze gamification of CS Education and compare with this study's results, but it will suggest the development direction of gamification in CS education in Korea.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.15 no.6
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• pp.579-589
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• 2004

The radar signals are generally very sensitive to relative orientations between radar and target. Thus, the performance of a target recognition system significantly deteriorates as the region of aspect angles becomes broader. To address this difficulty, in this paper, we propose a method based on the multi-aspect information in order to improve the classification capability ever for a wide angular region. First, range profiles are used to extract feature vectors based on the central moments and principal component analysis(PCA). Then, a classifier with the use of multi-aspect information is applied to them, yielding an additional improvement of target recognition capability. There are two different strategies among the classifiers that can fuse the information from multi-aspect radar signals: independent methodology and dependent methodology. In this study, the performances of the two strategies are compared within the frame work of target recognition. The radar cross section(RCS) data of six aircraft models measured at compact range of Pohang University of Science and Technology are used to demonstrate and compare the performances of the two strategies.

• Journal of the Korean Geosynthetics Society
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• v.16 no.4
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• pp.1-11
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• 2017

In this study, 1/6 small-scale model tests of helical piles were conducted to evaluate their installation time and ultimate capacities. Model sand layers were constructed using sand pluviating method to produce uniform soil relative density. For installation of different helical piles varying locations (vertical center-to-center spacings of 50 mm and 150 mm) of helix plates, two different rotation speeds of 15 rpm and 30 rpm were implemented. Cone penetration equipment was installed within the hallow section of the helical pile to increase ultimate capacity of helical pile and to evaluate soil properties of plugged soils and soils below pile tip after installation of the piles. Based on the test results, the most fasted installation was possible under the condition of "rotation speed of 30 rpm and center-to-center spacing of 50 mm", and the highest ultimate capacity was mobilized under the condition of "rotation speed of 30 rpm and center-to-center spacing of 150 mm with cone penetration implementation."

• Steel and Composite Structures
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• v.1 no.1
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• pp.17-32
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• 2001

The Rosette-joining system is a completely new press-joining method for cold-formed steel structures. One Rosette-joint has a shear capacity equal to that of approximately four screws or rivets. The Rosette thin-walled steel truss system presents a new fully integrated prefabricated alternative to light-weight roof truss structures. The trusses are built up on special industrial production lines from modified top hat sections used as top and bottom chords and channel sections used as webs which are joined together with the Rosette press-joining technique to form a completed structure easy to transport and install. A single web section is used when sufficient but can be strengthened by double-nesting two separate sections or by using two lateral profiles where greater compressive axial forces are met. An individual joint in the truss can be strengthened by introducing a hollow bolt into the joint hole. The bolt gives the connection capacity a boost of approximately 20%. A series of laboratory tests have been carried out in order to verify the Rosette truss system in practice. In addition to compression tests on individual sections of different lengths, tests have also been done on small structural assemblies and on actual full-scale trusses of a span of 10 metres. Design calculations have been performed on selected roof truss geometries based on the test results, FE-analysis and on the Eurocode 3 and U.S.(AISI) design codes.