• 정보교육학회논문지
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• 제8권4호
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• pp.523-534
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• 2004

본 연구에서는 상담 비전문가인 교사에게 상담 내용과 방법을 제공하여 인터넷 중독 문제를 가지고 있는 학생을 상담할 수 있도록 지원하는 시스템 모형을 개발하였다. 시스템은 상담 내용과 방법을 제공하는 상담 컨텐츠, 학생 정보와 상담 결과를 기록하고 조회할 수 있는 상담 지원, 인터넷 중독 진단 및 설문 평가를 위한 평가 지원의 세 가지 모듈로 구성된다. 개발된 시스템을 초.중등학교 교사 30명에게 실험 운영하여 인터넷 중독 문제가 있는 학생을 상담하여, 개발된 시스템 모형의 문제점을 분석하였으며, 그리고 상담에 참여한 교사들이 제기한 문제와 개선안을 토대로 시스템 모형을 수정하였다.

• Journal of Information Processing Systems
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• 제8권3호
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• pp.459-470
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• 2012

Recently, high-throughput technologies such as the two-hybrid system, protein chip, Mass Spectrometry, and the phage display have furnished a lot of data on protein-protein interactions (PPIs), but the data has not been accurate so far and the quantity has also been limited. In this respect, computational techniques for the prediction and validation of PPIs have been developed. However, existing computational methods do not take into account the fact that a PPI is actually originated from the interactions of domains that each protein contains. So, in this work, the information on domain modules of individual proteins has been employed in order to find out the protein interaction relationship. The system developed here, WASPI (Web-based Assistant System for Protein-protein interaction Inference), has been implemented to provide many functional insights into the protein interactions and their domains. To achieve those objectives, several preprocessing steps have been taken. First, the domain module information of interacting proteins was extracted by taking advantage of the InterPro database, which includes protein families, domains, and functional sites. The InterProScan program was used in this preprocess. Second, the homology comparison with the GO (Gene Ontology) and COG (Clusters of Orthologous Groups) with an E-value of $10^{-5}$, $10^{-3}$ respectively, was employed to obtain the information on the function and annotation of each interacting protein of a secondary PPI database in the WASPI. The BLAST program was utilized for the homology comparison.

• 상하수도학회지
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• 제19권2호
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• pp.144-148
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• 2005

The industrial development of large scale deep bed filters has been a very important step in the process of drinking water production and more recently in the tertiary treatment of wastewater. The target of deep bed filtration is the retention is the retention of small particles generally smaller than 30 microns at relatively small concentration, generally less than 30 mg/l from natural water (surface water or aquifers) or secondary treated wastewater. The relation between the retention efficiency and the characteristics of the particles has been extensively studied experimentally and through different models of retention. During the last years the development of new technologies (fiber filter, membrane modules) lead to more intensive processes compared to conventional sand filtration. Fiber filters can combine intensification with a decrease in specific energy needed however they cannot be operated under gravity like sand filters. Membrane filters (UF or MF) are much more intensive and efficient than sand filters. The specific energy needed is not so high (about $0.1Kwh/M^3$) but is higher than sand or fiber filter. A Life Cycle Analysis (LCA) has to be made for a complete comparison between these technologies taking in account that the efficiency of particle retention obtained by membrane filters is unique.

• 한국전기전자재료학회논문지
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• 제35권2호
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• pp.184-189
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• 2022

In this paper, the fault current limiting operations of three-phase transformer type superconducting fault current limiter (SFCL) using double quench, which consisted of E-I iron core with three legs wound by primary and secondary windings and two superconducting modules (SCMs), were analyzed according to three-phase ground fault types. To verify the effective operation of the three-phase transformer type SFCL using double quench, the test circuit for three-phase ground faults was constructed, and the fault current tests were carried out. Through analysis on the fault current test results, the different fault current limiting characteristics of three-phase transformer type SFCL using double quench from three-phase transformer type SFCL using three SCMs were discussed.

• 상하수도학회지
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• 제37권6호
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• pp.325-334
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• 2023

Membrane bioreactor (MBR) provides the benefits on high effluent quality and construction cost without the secondary clarification. Despite of these advantages, fouling, which clogs the pore in membrane modules, affects the membrane life span and effluent quality. Studies on the laboratory scale MBR were focused on the control of particulate fouling, organic fouling and inorganic fouling. However, less studies were focused on the control of biofouling and microbial aspect of membrane. In the full scale operation, most MBR produces high effluent quality to meet the national permit of discharge regulation. In this study, the performance and microbial community analysis were investigated in two MBRs. As the results, the performance of organic removal, nitrogen removal, and phosphorus removal was similar both MBRs. Microbial community analysis, however, showed that Azonexus sp. and Propionivibrio sp. contributed to indirect fouling to cause the chemical cleaning in the DX MBR.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.27-28
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• 2014

Beauveria bassiana (Cordycipitaceae, Hypocreales, Ascomycota) is an anamorphic fungus having a potential to be used as a biological control agent because it parasitizes a wide range of arthropod hosts including termites, aphids, beetles and many other insects. A number of bioactive secondary metabolites (SMs) have been isolated from B. bassiana and functionally verified. Among them, beauvericin and bassianolide are cyclic depsipeptides with antibiotic and insecticidal effects belonging to the enniatin family. Non-ribosomal peptide synthetases (NRPSs) play a crucial role in the synthesis of these secondary metabolites. NRPSs are modularly organized multienzyme complexes in which each module is responsible for the elongation of proteinogenic and non-protein amino acids, as well as carboxyl and hydroxyacids. A minimum of three domains are necessary for one NRPS elongation module: an adenylation (A) domain for substrate recognition and activation; a tholation (T) domain that tethers the growing peptide chain and the incoming aminoacyl unit; and a condensation (C) domain to catalyze peptide bond formation. Some of the optional domains include epimerization (E), heterocyclization (Cy) and oxidation (Ox) domains, which may modify the enzyme-bound precursors or intermediates. In the present study, we analyzed genomes of B. bassiana and its allied species in Hypocreales to verify the distribution of NRPS-encoding genes involving biosynthesis of beauvericin and bassianolide, and to unveil the evolutionary processes of the gene clusters. Initially, we retrieved completely or partially assembled genomic sequences of fungal species belonging to Hypocreales from public databases. SM biosynthesizing genes were predicted from the selected genomes using antiSMASH program. Adenylation (A) domains were extracted from the predicted NRPS, NRPS-like and NRPS-PKS hybrid genes, and used them to construct a phylogenetic tree. Based on the preliminary results of SM biosynthetic gene prediction in B. bassiana, we analyzed the conserved gene orders of beauvericin and bassianolide biosynthetic gene clusters among the hypocrealean fungi. Reciprocal best blast hit (RBH) approach was performed to identify the regions orthologous to the biosynthetic gene cluster in the selected fungal genomes. A clear recombination pattern was recognized in the inferred A-domain tree in which A-domains in the 1st and 2nd modules of beauvericin and bassianolide synthetases were grouped in CYCLO and EAS clades, respectively, suggesting that two modules of each synthetase have evolved independently. In addition, inferred topologies were congruent with the species phylogeny of Cordycipitaceae, indicating that the gene fusion event have occurred before the species divergence. Beauvericin and bassianolide synthetases turned out to possess identical domain organization as C-A-T-C-A-NM-T-T-C. We also predicted precursors of beauvericin and bassianolide synthetases based on the extracted signature residues in A-domain core motifs. The result showed that the A-domains in the 1st module of both synthetases select D-2-hydroxyisovalerate (D-Hiv), while A-domains in the 2nd modules specifically activate L-phenylalanine (Phe) in beauvericin synthetase and leucine (Leu) in bassianolide synthetase. antiSMASH ver. 2.0 predicted 15 genes in the beauvericin biosynthetic gene cluster of the B. bassiana genome dispersed across a total length of approximately 50kb. The beauvericin biosynthetic gene cluster contains beauvericin synthetase as well as kivr gene encoding NADPH-dependent ketoisovalerate reductase which is necessary to convert 2-ketoisovalarate to D-Hiv and a gene encoding a putative Gal4-like transcriptional regulator. Our syntenic comparison showed that species in Cordycipitaceae have almost conserved beauvericin biosynthetic gene cluster although the gene order and direction were sometimes variable. It is intriguing that there is no region orthologous to beauvericin synthetase gene in Cordyceps militaris genome. It is likely that beauvericin synthetase was present in common ancestor of Cordycipitaceae but selective gene loss has occurred in several species including C. militaris. Putative bassianolide biosynthetic gene cluster consisted of 16 genes including bassianolide synthetase, cytochrome P450 monooxygenase, and putative Gal4-like transcriptional regulator genes. Our synteny analysis found that only B. bassiana possessed a bassianolide synthetase gene among the studied fungi. This result is consistent with the groupings in A-domain tree in which bassianolide synthetase gene found in B. bassiana was not grouped with NRPS genes predicted in other species. We hypothesized that bassianolide biosynthesizing cluster genes in B. bassiana are possibly acquired by horizontal gene transfer (HGT) from distantly related fungi. The present study showed that B. bassiana is the only species capable of producing both beauvericin and bassianolide. This property led to B. bassiana infect multiple hosts and to be a potential biological control agent against agricultural pests.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2009년도 정기총회 및 추계학술대회 논문집
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• pp.105-110
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• 2009

The On Line Electric Vehicles(OLEV) that can pick up inductive power from underground coils on driving with high efficiency have been developed this year, and is now proposed in this paper. The IPS(Inductive Power Supply) system consists of power supply inverters, power supply rails, pick up modules, and a regulator. There are 3 generations of IPS have been developed so far, and the $4^{th}$ generation IPS is being developed. The $1^{st}$ generation has been demonstrated this Feb. 27, which is equipped with mechanically auto tracking pick-up module with 1cm air gap, and showed 80% power efficiency. The $2^{nd}$ generation IPS applied to an 120kW (average)/240kW(peak) motor powered electric bus has 17cm air gap with 72% power efficiency. For the $2^{nd}$ generation IPS, the Power supply inverter has 440V, 3phase input and 200A @ 20kHz output. The test power supply rail of 240m long is segmented by 60m each, where newly developed core structure and power cable are constructed under the road covered with asphalt of 5cm thickness. The pick-up modules which consist of core, winding wire, and rectifiers are fixed to the bottom of the bus which can carry more than 40 passengers and can pick up max. 60kW. To remove parasitic component and to transfer maximum power between them resonant circuit topology is applied to the primary and secondary sides. The EMF level is below 62.5mG at 1.75m from the center of the road to meet the regulation. Several effective ways of reducing EMF levels have been developed. In addition, effective ways to solve problems related high frequency power cables buried in ground and it's proof from soil have been studied also. This development shows that the IPS system is capable of supplying enough power to the pick-up of OLEV and can reduce battery size, weight and cost, which means the IPS with OLEV is one of the best candidate for EV.

• 한국정보과학회논문지:시스템및이론
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• 제36권3호
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• pp.171-180
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• 2009

바이트 단위 임의 접근이라는 램 특성과, 비휘발성이라는 디스크의 특성을 동시에 제공하는 FeRAM, MRAM, PRAM등의 스토리지 클래스 램(Storage Class Random Access Memory, SCRAM)이 소형 임베디드 시스템을 중심으로 점차 그 활용범위를 넓혀가고 있다. 본 논문에서는 SCRAM을 주기억 장치 및 보조 기억 장치로서 동시에 사용할 수 있는 차세대 통합 소프트웨어 구조를 제안한다. 제안된 구조는 크게 스토리지 클래스 램 드라이버(SCRAM Driver)와 스토리지 클래스 램 관리자(SCRAM Manager)로 구성된다. SCRAM Driver는 SCRAM을 직접 관리하며, FAT이나 Ext2와 같은 전통적인 파일 시스템이나 버디 할당자와 같은 전통적인 메모리 관리자, 혹은 SCRAM Manager 등의 상위 소프트웨어 계층에 저수준 인터페이스를 제공한다. SCRAM Manager는 파일 객체와 메모리 객체를 통합하여 관리함으로써 이들 간에 부가적인 비용이 없는 변환을 가능케 한다. 제안된 기법은 FeRAM이 장착된 실제 시스템에서 실험되었으며, 실험 결과를 통해 SCRAM Driver가 효율적으로 전통적인 파일시스템과 메모리 관리자가 요구하는 기능을 제공할 수 있음을 보였다. 또한 기존의 파일 시스템과 메모리 관리자를 통해 각각 SCRAM을 접하는 경우보다 SCRAM Manager가 수십 배 빠른 성능을 보임을 확인할 수 있었다.

• 전력전자학회논문지
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• 제18권4호
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• pp.312-321
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• 2013

In this paper, high efficiency control method for flyback inverter with synchronous rectifier(SR) based on photovoltaic AC modules is proposed. In this control method, the operation of SR is classified according to the voltage spike across main switch SP. When the voltage spike across SP is lower than the rating voltage of SP, the operation of active clamp circuit is interrupted for reducing the switching loss of auxiliary switch. In this time, the SR is operated for soft-switching of SP. When the voltage spike across Sp is higher than the rating voltage of SP, the operation of active circuit is activated for reducing the voltage spike. The SR is operated for reducing the conduction loss of secondary output diode. Thus, a switching loss of the main switch can be reduced in low power region, and weighted-efficiency can be improved. A theoretical analysis and the design principle of the proposed method are provided. And validity is confirmed through simulation and experimental results.

• The Plant Pathology Journal
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• 제28권3호
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• pp.227-238
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• 2012

Mitogen-activated protein kinase (MAPK) cascades are conserved signaling modules in the eukaryotic cells. They are involved in many major cell processes in fungi such as stress responses, vegetative growth, pathogenicity, secondary metabolism and cell wall integrity. In this review, we summarized the advances of research on the MAPK signaling pathways in Alternaria species. As major phytopathogenic fungi, Alternaria species reduce crop production. In contrast to the five MAPK pathways known in yeast, only three MAPK pathways as Fus3/Kss1-type, Hog1-type, and Slt2-type have been characterized in Alternaria. The Fus3/Kss1-type MAPK pathway participates in regulation of vegetative growth, conidiation, production of some cell-wall-degrading enzymes and pathogenicity. The Hog1-type pathway is involved in osmotic and oxidative stress, fungicides susceptibility and pathogenicity. The Slt2-type MAP kinases play an important role on maintaining cell wall integrity, pathogenicity and conidiation. Although recent advances on the MAPK pathways in Alternaria spp. reveal many important features on the pathogenicity, there are many unsolved problems regarding to the unknown MAP kinase cascade components and network among other major signal transduction. Considering the economic loss induced by Alternaria spp., more researches on the MAPK pathways will need to control the Alternaria diseases.