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Identification of GATA2 and AP-1 Activator Elements within the Enhancer VNTR Occurring in Intron 5 of the Human SIRT3 Gene

  • Bellizzi, Dina;Covello, Giuseppina;Di Cianni, Fausta;Tong, Qiang;De Benedictis, Giovanna
    • Molecules and Cells
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    • v.28 no.2
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    • pp.87-92
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    • 2009
  • Human SIRT3 gene contains an intronic VNTR enhancer. A T > C transition occurring in the second repeat of each VNTR allele implies the presence/absence of a putative GATA binding motif. A partially overlapping AP-1 site, not affected by the transition, was also identified. Aims of the present study were: 1) to verify if GATA and AP-1 sites could bind GATA2 and c-Jun/c-Fos factors, respectively; 2) to investigate whether such sites modulate the enhancer activity of the SIRT3-VNTR alleles. DAPA assay proved that GATA2 and c-Jun/c-Fos factors are able to bind the corresponding sites. Moreover, co-transfection experiments showed that the over-expression of GATA2 and c-Jun/c-Fos factors boosts the VNTR enhancer activity in an allelic-specific way. Furthermore, we established that GATA2 and c-Jun/c-Fos act additively in modulating the SIRT3-VNTR enhancer function. Therefore, GATA2 and AP-1 are functional sites and the T > C transition of the second VNTR repeat affects their activity.

Effects of A-site Ca and B-site Zr Substitution on the Dielectric Characteristics and Microstructure of BaTiO3-CaTiO3 Composite (A-site Ca 및 B-site Zr 첨가에 의한 BaTiO3-CaTiO3복합체의 유전특성 및 미세구조에 미치는 영향)

  • 윤만순;박영민
    • Journal of the Korean Ceramic Society
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    • v.40 no.1
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    • pp.37-45
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    • 2003
  • The dielectric and sintering characteristics of composites made by substituting Ca ion to Ba-site and Zr ion to Ti site in $(Ba{1-x}Ca_x)(Ti{0.96-yZr_ySn_{0.04})O_3$ $(0.15{\leq}x{\leq}0.20,\;0.09{\leq}y{\leq}0.14)$ were investigated. As the content of Ca was more than 15 mol%, composite was formed by precipitating the second phase whose main element was $CaTiO_3$ and the fraction of the second phase was increased. The curie temperature of composites was depended on Ca concentration, $-1.7^{\circ}C$ per mol% and the maximum dielectric constant of composite was decreased by the rate of 200/mol%. The substitution of Zr ion decreased the curie temperature by the rate of $10^{\circ}C$ per mol% and the maximum dielectric constant was decreased by 217/mol% due to the increase of diffuse phase transition. The density and insulation breakdown characteristics were improved by suppressing the abnormal grain growth due to the increase of second phase. We developed the composition of Y5U (EIA standard) condenser which had high breakdown voltage and dielectric constant by controlling diffuse phase transition by the addition of Zr ion into composite.

A Study on the Dwelling Site Planning in the Residential Improvement District as a Sustainable Development - Focused on the Nanminchon II District in Jeonju - (지속가능한 개발로서 주건환경개선지구내 주거지계획에 관한 연구)

  • Kim, In-Sun;Jihn, Jung
    • Journal of the Korean housing association
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    • v.18 no.3
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    • pp.73-80
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    • 2007
  • The purpose of this study is to suggest that dwelling site planning of residential improvement district is to make in the point of sustainable improvement. The methods of this study are as follow, first, introduce the concept of sustainable improvement and research possibility of adaption of the concept. second, make a survey of the Nanminchon II District in Jeonju as a sample of residential improvement district and make a comparative study of the before and after the development. third, research adaptable elements of sustainable residence concept to Nanminchon II District to dwelling site planning. Progress and result of residential improvement district affects dweller to character of place and habitation and will gain summits of architecture.

A Study on the Relationship of Visual Aesthetics Design and Performance in the Internet Web Site (인터넷 웹사이트의 시각적 디자인과 성과와의 관계에 관한 연구)

  • Kim, Seung Kyung
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.4 no.4
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    • pp.85-101
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    • 2008
  • This study focuses on visual aesthetic factors in the apparel web site design. 103 undergraduates participated in the evaluation of six internet web sites. The evaluation were recorded and analyzed by using the inspection method and a questionnaire. Findings of this study can be summarized as follows: First, the result of SPSS-factor analysis shows that there are 2 distinct factors; 'classical aesthetics' and 'expressive aesthetics'. 'classical aesthetics' and 'expressive aesthetics' can be described by visual aesthetic design. This conceptual confusion relating to 'visual aesthetic design' can be clarified by these findings. Second, as a result of multiple regression analyses, 'classical aesthetics' and 'expressive aesthetics' have a positive influence on 'interactivity' and 'web site evaluation'. This research clarifies the concepts of 'engagement' of Rosen & Purinton as the 'interactivity' between users and web sites. Finally, this study suggests that 'good design' for internet web sites depends on understanding how to attain the appropriate balance between 'classical aesthetics' and 'expressive aesthetics', based on the target customer.

A Study of Residents' Demand for External Space of Apartment Site (아파트 외부공간 실태와 조성기법)

  • 김한수
    • Journal of the Korean housing association
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    • v.11 no.3
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    • pp.87-98
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    • 2000
  • This study derives problems of external space utilization site from on-site observation and questionnaire survey in order to suggest solutions for the problems. The main results are follows. First, fences as visual boundaries of apartment site play some negative roles - separation of space and heterogeneity of circumstance. This study suggests green fences to cure this problem. Second, residents want to change space use - ground to be used as green space or space for relaxation in stead of parking lots, elevator hall as social space, and underground as bike parks or health centers. Third, rooftop space, used as empty space or machine storage space, should be designed to be utilized as relaxation space by making easy to enter and exit.

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A Study on the Planning for Geochang-gun High Technology Industrial Complex (거창군 첨단사업단지 계획방향에 관한 연구)

  • Kim, Kang Sub;Kim, Young Kyou;Lee, Sang Jung
    • Journal of the Korean Institute of Rural Architecture
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    • v.7 no.1
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    • pp.11-18
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    • 2005
  • This study analyzes the feasibility of constructing Geochang high technology industrial complex and present s the site, plan and development strategies. As a result, first, the Wallpyung area, superior form others in site characteristics, accessibility, surrounding facilities, was decided to the plan site. Second, the plan tries to adapt in the environment and maximizes the spatial link and user conveniences. Third, a transportation plan includes a circulation street network that adapt in topography, and considered future traffic situation and accessibility, hierarchy of road etc. Fourth, it also plans to locate a environment-friendly central park in the middle of site and buffer areas along at the outer sites and both sides of the roads. Fifth, for the development, it is advised to construct a public institution which manages the whole project and it is necessary to build a cooperate system.

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A Study on the Site Information of Urban (도시의 지형적 입지정보에 관한 연구)

  • Youn, In-Hyeok
    • The Journal of Information Technology
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    • v.10 no.2
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    • pp.1-9
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    • 2007
  • This study analyzed major cities of Chung-cheong area in Korea, -Daejeon($36^{\circ}11'{\sim}36^{\circ}30'\;N$, $127^{\circ}15'{\sim}127{\circ}34'\;E$), Chungju($36^{\circ}54'{\sim}37{\circ}02'\;N$, $127^{\circ}52'{\sim}128^{\circ}02'\;E$), and Cheongju($36^{\circ}34'{\sim}36^{\circ}44'\;N$, $127^{\circ}22'{\sim}127^{\circ}34'\;E$)- their characteristics as a Information Site. The geology map, a summit level map, and a drainage network map are created and analyzed the Site. The results are as follows : First, the geology of these cities are related with the shapes and the characteristics of the inner basins. The relief of inner basin determines by the characteristics of the bedrocks. Second, the major cities are close to rivers and the size of the granite & alluvium area influences the relief energy of the urban areas. Finally, the drainage networks explains the process involved in forming the basin and development of urban area.

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A Study on Alkyl Benzene Sulfonate Contamination in the Han River (한강수계(漢江水系)의 Alkyl Benzene Sulfonate 오염(汚染)에 관(關)한 연구(硏究))

  • Jung, Ki-Hwa
    • Journal of Pharmaceutical Investigation
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    • v.8 no.3
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    • pp.11-15
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    • 1978
  • The contamination concentrations of the alkyl benzene sulfonate (ABS) pollution in the Han river were investigated at 3 sites from July through september, 1977. The samples were taken on the upper, the middle, and the lower Han river and 5points of each part, respectively. The contamination concentrations of ABS pollution in the river were 0.012 p.p.m of the upper site, 0.206 p.p.m of the middle site, and 0.486 p.p.m of the lower site in average, respectively. Consequently, the contamination concentrations of ABS pollution in the Han river were the highest at the lower site, where is between second Han river bridge and Nan Ji island.

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Dynamic simulation models for seismic behavior of soil systems - Part II: Solution algorithm and numerical applications

  • Sahin, Abdurrahman
    • Geomechanics and Engineering
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    • v.9 no.2
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    • pp.169-193
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    • 2015
  • This paper is the second part of the study for determining the seismic behavior of soil systems. The aim of this part is to present solution approaches for determining seismic site amplification. For this purpose, two solution techniques are used. The first technique is equivalent linear analysis which is mostly used in literature. The other technique is real time parameter updating approach and this approach uses the possibilities of Simulink effectively. A graphical user interfaced (GUI) program called DTASSA standing for Discrete-Time Analysis of Seismic Site Amplification is developed. In DTASSA, automatic block diagram producing system is developed and seismic site amplification for multiple soil layers may easily be investigated in real time. Numerical applications have been carried out to check the reliability of developed algorithm. The results of DTASSA are compared with SUA, EERA and NERA programs for the particular example problems.

The Flow rate estimation of CSOs using EC Data (전기전도도를 이용한 CSO의 유량 추정)

  • Choi, Weon-Suk;Song, Chang-Soo
    • Journal of Korean Society of Water and Wastewater
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    • v.25 no.5
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    • pp.751-757
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    • 2011
  • The monitoring technique based on electrical conductivity (EC) can provide researchers with some advantages in maintenance management and is cost-effective as compared with existing CSOs monitoring. In this study, the flow rate estimation using EC data was executed in two sites where storm overflow chamber had installed. In the result of A-site, R2 of second order multinomial between dilution ratio of EC and observed flow rate was showed the range of 0.68 ~ 0.77. And $R^{2}$ of B-site was 0.62 ~ 0.81. On the other hand, cumulative frequency of A-site was 43.4 ~ 52.2% in the relative error level of under 20%. And B-site was 10.1 ~ 46.5%. The flow rate estimation formula was improved through consideration of some parameters including antecedent dry days and rainfall duration. And difference between estimated flow rate and observed flow rate in total rainfall event was very small.