• 한국응용곤충학회지
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• 제22권1호
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• pp.41-44
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• 1983

본 실험은 내충성품종을 선별하는 데 방사성동위원소인 P-32의 이용성을 검토하기 위해서 흰등멸구에 대해 기왕에 저항성 및 감수성으로 판정되어 있는 10개 품종을 선별하여 섭식량과 식이선호성간의 관계를 조사중 다음과 같은 결과를 얻었다. 1) 약충의 식이선호도와 P-32 섭식량간에는 밀접한 관계가 있었다. 2. 수칭유묘를 표식할 때는 근부와 엽의 길이 및 수를 일정하게 한 후 침적시키는 것이 P-32의 균일성을 인정할 수 있었다. 3. 내충성 검정시 공시충은 성충을 이용하는 것이 약충을 이용하는 것 보다 품종간에 상대적인 비교가 용이하였다.

• Journal of Nutrition and Health
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• 제35권10호
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• pp.1053-1059
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• 2002

In post-genome period, the technique for identifying gene expression has been changed to high throughput screening. In the field of molecular nutrition, the need for this technique to clarify molecular function of the specific nutrient is essential. In this study, we have tested the zinc-regulated gene expression in zinc-deficient U937 cells, using cDNA microarray which is the cutting-edge technique to screen large numbers of gene expression simultaneously. The study result can be used for the preliminary gene screening data for clarifying, using monocyte U937 cell line, molecular Zn aspect in atherosclerosis. U937 cells were cultured in Zn-adequate (control, 12 $\mu$M Zn) or Zn-deficient (experimental, 0 $\mu$M Zn) ESMI media during 2 days, respectively. Cells were harvested and RNA was extracted. Total RNA was reverse-transcriptinized and synthesized cDNA probe labeled with Cy-3. fluorescent labeled cDNA probe was applied to microarray slide for hybridization slide, and after then, the slide was scanned using fluorescence scanner. ‘Highly expressed genes’ in Zn-deficient U937 cells, comparing to Zn-adequate group, are mainly about the genes for motility protein, immune system protein, oncogene and tumor suppressor and ‘Less highly expressed genes’ are about the genes for transcription, apoptosis associated protein, cell cycle, and several basic transcription factors. The results of this preliminary study imply the effectiveness of cDNA microarray for expression profiling of a singly nutrient deficiency, specially Zn. Furthur study, using tailored-cDNA array and capillary endothelial cell lines, would be beneficial to clarify molecular Zn function, more in detail.

• Journal of Veterinary Science
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• 제21권1호
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• pp.16.1-16.10
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• 2020

Cancer is a major cause of death in dogs worldwide, and the incidence of cancer in dogs is increasing. The attenuated total reflection Fourier transform infrared spectroscopic (ATR-FTIR) technique is a powerful tool for the diagnosis of several diseases. This method enables samples to be examined directly without pre-preparation. In this study, we evaluated the diagnostic value of ATR-FTIR for the detection of cancer in dogs. Cancer-bearing dogs (n = 30) diagnosed by pathologists and clinically healthy dogs (n = 40) were enrolled in this study. Peripheral blood was collected for clinicopathological diagnosis. ATR-FTIR spectra were acquired, and principal component analysis was performed on the full wave number spectra (4,000-650 cm^-1). The leave-one-out cross validation technique and partial least squares regression analysis were used to predict normal and cancer spectra. Red blood cell counts, hemoglobin levels and white blood cell counts were significantly lower in cancer-bearing dogs than in clinically healthy dogs (p < 0.01, p < 0.01 and p = 0.03, respectively). ATR-FTIR spectra showed significant differences between the clinically healthy and cancer-bearing groups. This finding demonstrates that ATR-FTIR can be applied as a screening technique to distinguish between cancer-bearing dogs and healthy dogs.

• Journal of Microbiology and Biotechnology
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• 제24권11호
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• pp.1559-1565
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• 2014

Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosalicylic acid (DNS) method for microwell plate-based screening. Targeted microbial samples were initially cultured on agar plates with both cellulose and xylan as substrates. Then, isolated colonies were subcultured in broth media containing yeast extract and either cellulose or xylan. The supernatants of the culture broth were tested with our modified DNS screening method in a 96-microwell plate, with a $200{\mu}l$ total reaction volume. In addition, the stability and reliability of glucose and xylose standards, which were used to determine the enzymatic activity, were studied at $100^{\circ}C$ for different time intervals in a dry oven. It was concluded that the minimum incubation time required for stable color development of the standard solution is 20 min. With this technique, we successfully screened 21 and 31 cellulase- and xylanase-producing strains, respectively, in a single experimental trial. Among the identified strains, 19 showed both cellulose and xylan hydrolyzing activities. These microbes can be applied to bioethanol production from cellulosic and hemicellulosic biomass.

• Clinical and Experimental Reproductive Medicine
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• 제38권3호
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• pp.126-134
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• 2011

Preimplantation genetic diagnosis (PGD) is gradually widely used in prevention of gene diseases and chromosomal abnormalities. Much improvement has been achieved in biopsy technique and molecular diagnosis. Blastocyst biopsy can increase diagnostic accuracy and reduce allele dropout. It is cost-effective and currently plays an important role. Whole genome amplification permits subsequent individual detection of multiple gene loci and screening all 23 pairs of chromosomes. For PGD of chromosomal translocation, fluorescence $in-situ$ hybridization (FISH) is traditionally used, but with technical difficulty. Array comparative genomic hybridization (CGH) can detect translocation and 23 pairs of chromosomes that may replace FISH. Single nucleotide polymorphisms array with haplotyping can further distinguish between normal chromosomes and balanced translocation. PGD may shorten time to conceive and reduce miscarriage for patients with chromosomal translocation. PGD has a potential value for mitochondrial diseases. Preimplantation genetic haplotyping has been applied for unknown mutation sites of single gene disease. Preimplantation genetic screening (PGS) using limited FISH probes in the cleavage-stage embryo did not increase live birth rates for patients with advanced maternal age, unexplained recurrent abortions, and repeated implantation failure. Polar body and blastocyst biopsy may circumvent the problem of mosaicism. PGS using blastocyst biopsy and array CGH is encouraging and merit further studies. Cryopreservation of biopsied blastocysts instead of fresh transfer permits sufficient time for transportation and genetic analysis. Cryopreservation of embryos may avoid ovarian hyperstimulation syndrome and possible suboptimal endometrium.

• Genomics & Informatics
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• 제19권1호
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• pp.6.1-6.10
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• 2021

Vascular endothelial growth factor (VEGF) is expressed at elevated levels by most cancer cells, which can stimulate vascular endothelial cell growth, survival, proliferation as well as trigger angiogenesis modulated by VEGF and VEGFR (a tyrosine kinase receptor) signaling. The angiogenic effects of the VEGF family are thought to be primarily mediated through the interaction of VEGF with VEGFR-2. Targeting this signaling molecule and its receptor is a novel approach for blocking angiogenesis. In recent years virtual high throughput screening has emerged as a widely accepted powerful technique in the identification of novel and diverse leads. The high resolution X-ray structure of VEGF has paved the way to introduce new small molecular inhibitors by structure-based virtual screening. In this study using different alkaloid molecules as potential novel inhibitors of VEGF, we proposed three alkaloid candidates for inhibiting VEGF and VEGFR mediated angiogenesis. As these three alkaloid compounds exhibited high scoring functions, which also highlights their high binding ability, it is evident that these alkaloids can be taken to further drug development pipelines for use as novel lead compounds to design new and effective drugs against cancer.

• Journal of Electrical Engineering and Technology
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• 제10권4호
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• pp.1899-1909
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• 2015

Glaucoma is one of the most common causes of blindness which is caused by increase of fluid pressure in the eye which damages the optic nerve and eventually causing vision loss. An automated technique to diagnose glaucoma disease can reduce the physicians’ effort in screening of Glaucoma in a person through the fundal retinal images. In this paper, optimal hyper analytic wavelet transform for Glaucoma detection technique from fundal retinal images is proposed. The optimal coefficients for transformation process are found out using the hybrid GSO-Cuckoo search algorithm. This technique consists of pre-processing module, optimal transformation module, feature extraction module and classification module. The implementation is carried out with MATLAB and the evaluation metrics employed are accuracy, sensitivity and specificity. Comparative analysis is carried out by comparing the hybrid GSO with the conventional GSO. The results reported in our paper show that the proposed technique has performed well and has achieved good evaluation metric values. Two 10- fold cross validated test runs are performed, yielding an average fitness of 91.13% and 96.2% accuracy with CGD-BPN (Conjugate Gradient Descent- Back Propagation Network) and Support Vector Machines (SVM) respectively. The techniques also gives high sensitivity and specificity values. The attained high evaluation metric values show the efficiency of detecting Glaucoma by the proposed technique.

• Journal of Plant Biotechnology
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• 제29권4호
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• pp.235-240
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• 2002

현재 유전자 변형 작물의 검정에는 CaMV 35S프로모터 혹은 NOS 터미네이터 등과 같이 형질전환에 널리 이용하는 유전인자를 검출하기 위한 PCR 기술이 널리 이용되고 있다. 본 연구에서는 유전자변형 감자를 검정하기 위한 새로운 기술로서 감자특이 internal control과 CaMV 35S 프로모터 혹은 NOS 터미네이터에 특이적인 프라이머를 이용한 경쟁적 이중PCR 방법을 개발하였다. 감자 유전자의 RAPD 결과 이용한 모든 품종에서 약 530 bp인 homozygous DNA 밴드를 증폭하는 특이 primer (rAGU4A)를 찾아 감자특이 internal control 증폭용으로 이용하였다. 이 프라이머에 의해 증폭되는 DNA는 TC 염기의 반복 빈도가 높은 repetitive 혹은 microsatellite DNA (AF541972)로 판단되었다. 이와 같은 단일 프라이머 internal control 증폭용으로 이용한 경쟁적 이중 PCR은 비특이적 PCR 산물을 줄일 수 있고, 기존 방법들에 비해 경제적이다. 현재 상품화되어 있는 유전자 변형 감자품종인 'New Leaf'의 경우도 형질전환에 이용한 유전인자로 CaMV 35S 프로모터와 NOS 터미네이터가 모두 이용되었으므로 이 기술을 이용할 경우 현재까지 상품화된 유전자 변형감자들의 효율적인 검정 기술로 이용될 수 있을 것으로 기대된다.

• 한국물환경학회지
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• 제33권3호
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• pp.348-358
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• 2017

The process of evaluating and selecting the best available techniques presents various characteristics for each country. In the case of EU, BAT is selected through TWG meeting after first screening, mass and energy balance, impact assessment and decision support process. Korea has proposed four principles to select BAT that can be carbon neutral for each environmental infrastructure in order to reduce greenhouse gas emissions. In order to evaluate and select the best available technique, it is necessary to differentiate the method according to whether it is a technique generally applied at the current workplace, whether it is a single technique or a combination technique, and whether it is a technology technique or management technique. In the case of a single technique, it should be evaluated whether it is a technique applied in the workplace, excessive cost, superior environmental technique over BAT, and secondary environmental pollution. In the case of multiple techniques, it is necessary to examine whether the emission standards are met and whether the pollutants can be treated at the same level as BAT. In the case of BAT candidates for management techniques, whether or not they contribute directly or indirectly to lowering the emission level of pollutants can be an important evaluation item. In the case of environmental techniques that are not generally applied in the workplace, it is recommended that the following 8 steps be carried out, including those prescribed by law. In the first stage, the list of performance evaluation factors is listed. In the second stage, the level of disposal of pollutants and the level of satisfaction with standards are listed. In the third stage, the environmental evaluation elements are listed. In the fourth stage, Is to list the economic evaluation elements, step 6 is to list the pollution and accident prevention evaluation factors, step 7 is the quantitative evaluation of the technical working group, and step 8 is BAT confirmation through deliberation of the central environmental policy committee.

• Transactions on Electrical and Electronic Materials
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• 제13권5호
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• pp.225-228
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• 2012

In order to synthesize LED phosphor materials, we have applied three novel synthesis techniques, "melt synthesis", "fluidized bed synthesis" and "vapor-solid hybrid synthesis", in contrast with the conventional solid state reaction technique. These synthesis techniques are also a general and powerful tool for rapid screening and improvements of new phosphor materials.