Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Attenuated total reflection Fourier transform infrared as a primary screening method for cancer in canine serum

  • Macotpet, Arayaporn (Division of Companion Animal Medicine, Faculty of Veterinary Medicine, Khon Kaen University) ;
  • Pattarapanwichien, Ekkachai (Division of Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University) ;
  • Chio-Srichan, Sirinart (Synchrotron Light Research Institute, University Avenue) ;
  • Daduang, Jureerut (Centre for Research and Development of Medical Diagnostic Laboratories, Biosensor Research Group for Non-Communicable Disease and Infectious Disease, Faculty of Associated Medical Sciences, Khon Kaen University) ;
  • Boonsiri, Patcharee (Department of Biochemistry, Faculty of Medicine, Khon Kaen University)
  • Received : 2019.01.24
  • Accepted : 2019.11.09
  • Published : 2020.01.31
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Abstract

Cancer is a major cause of death in dogs worldwide, and the incidence of cancer in dogs is increasing. The attenuated total reflection Fourier transform infrared spectroscopic (ATR-FTIR) technique is a powerful tool for the diagnosis of several diseases. This method enables samples to be examined directly without pre-preparation. In this study, we evaluated the diagnostic value of ATR-FTIR for the detection of cancer in dogs. Cancer-bearing dogs (n = 30) diagnosed by pathologists and clinically healthy dogs (n = 40) were enrolled in this study. Peripheral blood was collected for clinicopathological diagnosis. ATR-FTIR spectra were acquired, and principal component analysis was performed on the full wave number spectra (4,000-650 cm-1). The leave-one-out cross validation technique and partial least squares regression analysis were used to predict normal and cancer spectra. Red blood cell counts, hemoglobin levels and white blood cell counts were significantly lower in cancer-bearing dogs than in clinically healthy dogs (p < 0.01, p < 0.01 and p = 0.03, respectively). ATR-FTIR spectra showed significant differences between the clinically healthy and cancer-bearing groups. This finding demonstrates that ATR-FTIR can be applied as a screening technique to distinguish between cancer-bearing dogs and healthy dogs.

Keywords

Acknowledgement

The authors are grateful to Miss Prangtip Uthaiwat for her technical assistance. Access to the ATR-FTIR spectrometer (Agilent Technologies) was kindly provided by the Center of Innovation and Standard Medical Technology and Physical Therapy (CISMAP), Faculty of Associated Medical Sciences, Khon Kaen University.

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