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Culture Characteristics and Optimal Conditions for Mycelial Growth of Calocybe indica (Calocybe indica의 배양적 특성과 균사 배양 적합 조건 설정)

  • Min, Gyeong-Jin;Park, Hea-sung;Lee, Een-ji;Lee, Chan-Jung
    • The Korean Journal of Mycology
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    • v.48 no.3
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    • pp.273-284
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    • 2020
  • Calocybe indica is an edible mushroom commercially cultivated in India and other tropical countries. In this study, the culture characteristics and optimal conditions of milky mushroom strains were determined. The growth temperature and pH range of milky mushrooms was extensively investigated between 15-35 ℃ and pH 3-11. For efficient cultivation, 20 types of nutrient sources were selected that consisted of one of 21 types of carbon sources, 6 organic nitrogen sources, 6 inorganic nitrogen sources, 13 amino acids, 6 organic acids and 12 inorganic salts. The impact of each of the selected nutrition sources and their concentration on growth was investigated. The optimum pH and temperature were determined to be pH 6.0 and 15 ℃, respectively. The optimum concentration of medium elements for the mycelial growth of C. indica was determined to be as follows: carbon source, 2% maltose; organic nitrogen source, 1% yeast extract; inorganic nitrogen source, 0.1% NaNO3; amino acid, 0.7% asparagine; organic acid, 0.07% acetic acid; inorganic salt, 0.07 mM MnSO4.

Prediction of Chemical Composition in Distillers Dried Grain with Solubles and Corn Using Real-Time Near-Infrared Reflectance Spectroscopy

  • Choi, Sung Won;Park, Chang Hee;Lee, Chang Sug;Kim, Dong Hee;Park, Sung Kwon;Kim, Beob Gyun;Moon, Sang Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.3
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    • pp.177-184
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    • 2013
  • This work was conducted to assess the use of Near-infrared reflectance spectroscopy (NIRS) as a technique to analyze nutritional constituents of Distillers dried grain with solubles (DDGS) and corn quickly and accurately, and to apply an NIRS-based indium gallium arsenide array detector, rather than a NIRS-based scanning system, to collect spectra and induce and analyze calibration equations using equipment which is better suited to field application. As a technique to induce calibration equations, Partial Least Squares (PLS) was used, and for better accuracy, various mathematical transformations were applied. A multivariate outlier detection method was applied to induce calibration equations, and, as a result, the way of structuring a calibration set significantly affected prediction accuracy. The prediction of nutritional constituents of distillers dried grains with solubles resulted in the following: moisture ($R^2$=0.80), crude protein ($R^2$=0.71), crude fat ($R^2$=0.80), crude fiber ($R^2$=0.32), and crude ash ($R^2$=0.72). All constituents except crude fiber showed good results. The prediction of nutritional constituents of corn resulted in the following: moisture ($R^2$=0.79), crude protein ($R^2$=0.61), crude fat ($R^2$=0.79), crude fiber ($R^2$=0.63), and crude ash ($R^2$=0.75). Therefore, all constituents except for crude fat and crude fiber were predicted for their chemical composition of DDGS and corn through Near-infrared reflectance spectroscopy.

The Effect of Representative Dataset Selection on Prediction of Chemical Composition for Corn kernel by Near-Infrared Reflectance Spectroscopy (예측알고리즘 적용을 위한 데이터세트 구성이 근적외선 분광광도계를 이용한 옥수수 품질평가에 미치는 영향)

  • Choi, Sung-Won;Lee, Chang-Sug;Park, Chang-Hee;Kim, Dong-Hee;Park, Sung-Kwon;Kim, Beob-Gyun;Moon, Sang-Ho
    • Journal of Animal Environmental Science
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    • v.20 no.3
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    • pp.117-124
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    • 2014
  • The objectives were to assess the use of near-infrared reflectance spectroscopy (NIRS) as a tool for estimating nutrient compositions of corn kernel, and to apply an NIRS-based indium gallium arsenide array detector to the system for collecting spectra and analyzing calibration equations using equipments designed for field application. Partial Least Squares Regression (PLSR) was employed to develop calibration equations based on representative data sets. The kennard-stone algorithm was applied to induce a calibration set and a validation set. As a result, the method for structuring a calibration set significantly affected prediction accuracy. The prediction of chemical composition of corn kernel resulted in the following (kennard-stone algorithm: relative) moisture ($R^2=0.82$, RMSEP=0.183), crude protein ($R^2=0.80$, RMSEP=0.142), crude fat ($R^2=0.84$, RMSEP=0.098), crude fiber ($R^2=0.74$, RMSEP=0.098), and crude ash ($R^2=0.81$, RMSEP=0.048). Result of this experiment showed the potential of NIRS to predict the chemical composition of corn kernel.

Optimal Fermentation Conditions for Enhanced Glutathione Production by Saccharomyces cerevisiae FF-8

  • Cha, Jae-Young;Park, Jin-Chul;Jeon, Beong-Sam;Lee, Young-Choon;Cho, Young-Su
    • Journal of Microbiology
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    • v.42 no.1
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    • pp.51-55
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    • 2004
  • The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF -8 was investigated. Glucose, yeast extract, KH$_2$PO$_4$, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH$_2$PO$_4$ 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.

A Very Early-Maturing Italian Ryegrass (Lolium multiflorum Lam.) New Variety, 'Green Farm' for Double Cropping System (이모작 작부체계에 적합한 극조생종 이탈리안 라이그라스 신품종 '그린팜'의 품종 특성)

  • Ji, Hee-Chung;Lee, Sang-Hyun;Yoon, Sei-Hyung;Kim, Ki-Yong;Choi, Gi-Jun;Park, Hyung-Soo;Park, Nam-Gun;Lim, Young-Chul;Lee, Eun-Sup
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.1
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    • pp.9-14
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    • 2011
  • This experiment was carried out to breed a very early maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan from 2009 to 2010. A new variety, 'Green farm' is a diploid variety with green in leaf color and has erect and semi-erect growth habit in late autumn and early spring, respectively. 'Green farm' was on 28 April in heading date as a early-maturing variety. 'Green farm' was also wider by 0.4 mm in flag leaf width, longer by 0.8 cm in flag leaf length and shorter by 5 cm in plant height than those of control variety, 'Florida 80', respectively. 'Green farm' was thicker in stem thickness and stronger in winter hardness than those of the 'Florida 80'. Dry matter (DM) yield (11,790 kg/ha) of 'Green farm' was similar to that of 'Florida 80'. In vitro dry matter digestibility (IVDMD), total digestible nutrient (TDN) and crude protein (CP) of 'Green farm' were 68.7, 63.3 and 10.3% which are 1.9, 1.7 and 0.6% higher than those of 'Florida 80', respectively. Acid detergent fiber (ADF) and neutral detergent fiber (NDF) of 'Green farm' were 32.5 and 54.6% which are 2.2 and 4.3% lower than those of the 'Florida 80', respectively.

Effects of dietary carbohydrases on productive performance and immune responses of lactating sows and their piglets

  • Lee, Jeong Jae;Choi, Seong Ho;Cho, Jin Ho;Choe, Jeehwan;Kang, Joowon;Kim, Soyun;Park, Sangwoo;Kyoung, Hyunjin;Seo, Dongoh;Cho, Jee-Yeon;Park, Il-Hun;Oh, Sangnam;Kim, Hyeun Bum;Song, Minho
    • Journal of Animal Science and Technology
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    • v.61 no.6
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    • pp.359-365
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    • 2019
  • This study was conducted to evaluate effects of dietary multi-carbohydrases (MCS) in a lactating sow diet on productive performance and immune responses of sows and their piglets. A total of 12 sows (218.37 ± 5.5 kg BW; 2 parity) were randomly assigned to 2 dietary treatments: a diet based on corn-soybean meal (CON) and CON with 0.01% MCS. The MCS contained xylanase (2,700 units/g), β-glucanase (700 units/g), and cellulase (800 units/g). Sows were fed the dietary treatments for 28 days (weaning) after farrowing. Blood samples were collected from sows on d 0, 3, and 7 after farrowing and randomly selected 2 nursing piglets in each sow on d 3, 7, and 14 after birth. Measurements were productive performance of sows, frequency of diarrhea of piglets, and immune responses of sows and their piglets. Sows fed MCS had lower (p < 0.05) their body weight change than those fed CON. Piglets from sows fed MCS had higher (p < 0.05) average weight gain and body weight at weaning day and lower (p < 0.10) frequency of diarrhea than those from sows fed CON. Sows fed MCS had lower number of white blood cells (WBC) on d 3 (p < 0.05) and TGF-β1 on d 7 (p < 0.10) during lactation than those fed CON. Similarly, piglets from sows fed MCS had also lower (p < 0.05) number of WBC on d 3 and d 7 and TGF-β1 and C-reactive protein on d 7 during lactation than those from sows fed CON. In addition, piglets from sows fed MCS had higher (p < 0.10) immunoglobulin G and M on d 7 during lactation those from sows fed CON. In conclusion, addition of dietary MCS in the lactating sow diet based on corn and soybean meal improved productive performance of sows and their litters and modulated their immune responses.

[ Hα ] SPECTRAL PROPERTIES OF VELOCITY THREADS CONSTITUTING A QUIESCENT SOLAR FILAMENT

  • Chae, Jong-Chul;Park, Hyung-Min;Park, Young-Deuk
    • Journal of The Korean Astronomical Society
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    • v.40 no.3
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    • pp.67-82
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    • 2007
  • The basic building block of solar filaments/prominences is thin threads of cool plasma. We have studied the spectral properties of velocity threads, clusters of thinner density threads moving together, by analyzing a sequence of $H{\alpha}$ images of a quiescent filament. The images were taken at Big Bear Solar Observatory with the Lyot filter being successively tuned to wavelengths of -0.6, -0.3, 0.0, +0.3, and +0.6 ${\AA}$ from the centerline. The spectra of contrast constructed from the image data at each spatial point were analyzed using cloud models with a single velocity component, or three velocity components. As a result, we have identified a couple of velocity threads that are characterized by a narrow Doppler width($\Delta\lambda_D=0.27{\AA}$), a moderate value of optical thickness at the $H{\alpha}$ absorption peak($\tau_0=0.3$), and a spatial width(FWHM) of about 1". It has also been inferred that there exist 4-6 velocity threads along the line of sight at each spatial resolution element inside the filament. In about half of the threads, matter moves fast with a line-of-sight speed of $15{\pm}3km\;s^{-1}$, but in the other half it is either at rest or slowly moving with a line-of-sight velocity of $0{\pm}3km\;s^{-1}$. It is found that a statistical balance approximately holds between the numbers of blue-shifted threads and red-shifted threads, and any imbalance between the two numbers is responsible for the non-zero line-of-sight velocity determined using a single-component model fit. Our results support the existence not only of high speed counter-streaming flows, but also of a significant amount of cool matter either being at rest or moving slowly inside the filament.

Effect of Age on Chemical Composition and Meat Quality for Loin and Top Round of Hanwoo Cow Beef (한우암소고기의 연령이 등심 및 우둔부위 일반성분 및 육질에 미치는 영향)

  • Cho, Soohyun;Seong, Pilnam;Kang, Geunho;Choi, Soonho;Chang, Sunsik;Kang, Sun Moon;Park, Kyung Mi;Kim, Youngchun;Hong, Sunggu;Park, Beom Young
    • Food Science of Animal Resources
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    • v.32 no.6
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    • pp.810-819
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    • 2012
  • This study investigated the chemical composition, meat quality and volatile flavor compounds in loin and top round of Hanwoo beef (n=126) depending on different age groups (G1, <5; G2, 6-8; G3, >9 years old). The intramuscular fat content (%) was higher for loin and top round of G1 (p<0.05) than that in the other groups. There was no difference in age groups for the top round; however, the loin of G1 had lower protein content (p>0.05). Total collagen content was lower in the top round of G3 (p<0.05). The loin and top round muscles of G1 had higher $a^*$ values and lower Warner Bratzler shear force values than that in the other age groups (p<0.05). The loin muscles of G1 were lower in percentage of cooking loss and higher in the water holding capacity than the loin in the other groups (p<0.05). The loin of G1 had lower total content of saturated fatty acids, whereas the top round of G1 had higher total content of monounsaturated fatty acids and lower total content of polyunsaturated fatty acids than that in the other age groups (p<0.05). Alanine was the highest free amino acid in the loin of Hanwoo beef, followed by glutamine, glycine, isoleucine and proline. The loin of G1 had higher contents of threonine, alanine, valine, methionine, phenylalanine, leucine and lysine than those in the other groups (p<0.05). The loin of G3 contained higher 3-methylbutanal, furfural, octanal, 1-(acetyloxy)-2-propanone, 1-octanol, 2,5-dimethylpyrazine and 2-ethyl-2,5-dimethylpyrazine in volatile flavor components than the loin in G1(p<0.05).

Diurnal Size Distributions of Black Carbon by Comparison of Optical Particulate Measurements - Part I (광학 입자 측정기 비교 측정에 의한 입경별 일평균 블랙카본 분석 - Part I)

  • Park, Da-Jeong;Lee, Kwang-Yul;Park, Kihong;Bae, Min-Suk
    • Journal of Korean Society for Atmospheric Environment
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    • v.32 no.1
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    • pp.1-8
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    • 2016
  • Optical particle sizer (OPS), optical particle counter (OPC), and aethalomter were deployed to measure the particle number concentrations from 0.3 to $10.0{\mu}m$ and black carbon (BC) concentrations. Comparisons of particle number concentrations measured by OPS and OPC were conducted to evaluate the performance of the each optical instrument at the Gwangju sampling site ($35.23^{\circ}N$, $126.84^{\circ}E$) for 14 days from Dec. 27 in 2014. Although a good correlation ($r^2=0.99$) between the OPS and OPC was observed for both the particle number and volume concentrations, different relationships by BC concentrations can be associated with the intensities by different light scattering angles. In addition, based on diurnal patterns of size distributions in 24 hr running correlation coefficient determination, BC concentrations were highly related to the particles less than $0.3{\mu}m$ observed in the morning traffic hour.

Physicochemical properties and β-glucan contents of Korean naked oat (Avena sativa L.) cultivars (국내 육성 쌀귀리 품종의 이화학 특성 및 베타글루칸 함량)

  • Lee, Mi-Ja;Park, Song-Yie;Kim, Yang-Kil;Kim, Hyung-Soon;Park, Hyoung-Ho;Lee, Yoon Jeong;Jeong, Heon Sang
    • Korean Journal of Food Science and Technology
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    • v.49 no.1
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    • pp.97-103
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    • 2017
  • Due to increased consumption and demand for oat-related foods and processed food, this study examined physicochemical properties, antioxidant activity, and ${\beta}-glucan$ contents of 5 Korean naked oat cultivars. Oat seeds had long-grain appearance in all cultivars except for Seonyang. The contents of main components such as protein, starch, and crude fat were significantly different among cultivars. The total ${\beta}-glucan$ content was 3.78-4.60% and the soluble ${\beta}-glucan$ ratio was 71-83%. Fatty acid composition was C18:1, C18:2, C16:0, C18:0, and C18:3. Unsaturated fatty acid (USFA) content was 75.4-81.2%. 2,2-Diphenyl-1-picrylhydrazil (DPPH) and 2,2'-azino-bis-3-ethyl-benzo-thiaxoline-6-sulfonic acid (ABTS) radical scavenging activity were significantly different for each cultivar. Daeyang had the highest ${\beta}-glucan$, USFA content, and antioxidant activity. Protein content showed a negative correlation with starch content (r = -0.775). Antioxidant activity was positively correlated with total phenol content (r =0.760). Ash content and flour whiteness showed a positive correlation (r =0.732).