• Journal of Environmental Health Sciences
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• v.39 no.4
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• pp.322-334
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• 2013

Objectives: This study describes the results of a mass screening and secondary questionnaire conducted among sampled residents around Camp Carroll. Methods: The subjects were sampled based on Waegwan groundwater ingestion history via a primary health questionnaire survey. However, the study population included voluntary participants and there were no grounds for disqualification. Among the 1,033 residents, excluding people living outside Waegwan, 844 subjects age 30 and over were analyzed. History of physician-diagnosed disease (including detailed history of cancer), eating habits, drinking and smoking histories were queried through questionnaires. Health screening consisted of a blood pressure check and blood test (complete blood cell, liver enzyme, lipid, blood sugar test, etc.). Results: The proportion of abnormal gamma-glutamyltransferase levels was higher in the groundwater ingesting female group than the non-ingesting female group. The odds ratios of the ingested '1 to 9 years' and '10 years and over' groups were 3.09 and 0.87, respectively. Proportions of hypertension in males, abnormal serum triglyceride levels in all and in females, and abnormal serum high density lipoprotein cholesterol levels in males were higher in the '10 to 29-year' resident group than in the '1 to 9 year' group. However, there were no significant trends according to length of residence. Conclusions: Physician-diagnosed prevalence and laboratory test results are not different by histories of Waegwan groundwater ingestion and by length of residence. Even if there are partially significant differences, they do not tend to follow increases of exposure amount and trends.

• The Journal of Korean Physical Therapy
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• v.21 no.3
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• pp.87-93
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• 2009

Purpose: TThis study examined the effect of repetitive magnetic stimulation (RMS) on the viability and proliferative response of human adipose tissue-derived stromal cells (hATSCs) in vitro. Methods: The hATSCs were cultured primarily from human adipose tissue harvested by liposuction and incubated in a $37^{\circ}C$ plastic chamber. The cells were exposed to a repetitive magnetic field using a customized magnetic stimulator (Biocon-5000, Mcube Technology). The RMS parameters were set as follows: repetition rate=10Hz, 25Hz (stimulus intensity 100%= 0.1 Tesla, at 4cm from the coil), stimulated time= 1, 5, and 20 minutes. Twenty four hours after one application of RMS, the hATSCs were compared with the sham stimulation, which were kept under the same conditions without the application of RMS. The cells were observed by optical microscopy to determine the morphology and assessed by trypan blue staining for cell proliferation. The apoptosis and viability of the hATSCs were also analyzed by fluorescence-activated cell sorting (FACS) analysis of Annexin V and MTT assay. Results: After RMS, the morphology of the hATSCs was not changed and the apoptosis of hATSCs were not increased compared to the sham stimulation. The viability of the cells was similar to the cells given the sham stimulation. Interestingly, the level of hATSC proliferation was significantly higher in all RMS groups. Conclusion: The application of RMS may not cause a change in morphology and viability of hATSCs but can increase the level of cell proliferation in vitro. RMS might be useful as an adjuvant tool in combination with stem cell therapy without adverse effects.

• Biomolecules & Therapeutics
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• v.19 no.2
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• pp.187-194
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• 2011

Atherosclerosis and post-angiography restenosis are associated with intimal thickening and concomitant vascular smooth muscle cell (VSMC) proliferation. Obovatol, a major biphenolic component isolated from the Magnolia obovata leaf, is known to have anti-inflammatory and anti-tumor activities. The goal of the present study was to enhance the inhibitory effects of obovatol to improve its potential as a preventive or therapeutic agent in atherosclerosis and restenosis. Platelet-derived growth factor (PDGF)-BB-induced proliferation of rat aortic smooth muscle cells (RASMCs) was examined in the presence or absence of a newly synthesized obovatol derivative, OD78. The observed anti-proliferative effect of OD78 was further investigated by cell counting and [$^3H$]-thymidine incorporation assays. Treatment with 1-4 ${\mu}M$ OD78 dose-dependently inhibited the proliferation and DNA synthesis of 25 ng/ml PDGF-BB-stimulated RASMCs. Accordingly, OD78 blocked PDGF-BB-induced progression from the $G_0/G_1$ to S phase of the cell cycle in synchronized cells. OD78 decreased the expression levels of CDK4, cyclin E, and cyclin D1 proteins, as well as the phosphorylation of retinoblastoma protein and proliferating cell nuclear antigen; however, it did not change the CDK2 expression level. In addition, OD78 inhibited downregulation of the cyclin-dependent kinase inhibitor (CKI) $p27^{kip1}$. However, OD78 did not affect the CKI $p21^{cip1}$ or phosphorylation of early PDGF signaling pathway. These results suggest that OD78 may inhibit PDGF-BB-induced RASMC proliferation by perturbing cell cycle progression, potentially through $p27^{kip1}$ pathway activation. Consequently, OD78 may be developed as a potential anti-proliferative agent for the treatment of atherosclerosis and angioplasty restenosis.

• Research in Community and Public Health Nursing
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• v.19 no.1
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• pp.27-35
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• 2008

Purpose: The purpose of this study was to examine the effects of aroma-inhalation on depression, sleep, mood, and smell in stroke patients and their caregivers. Method: This study employed one group pretest-posttest repeated design. Data were collected at three time points: baseline(pre-test), 10 days and 20 days after the intervention(Post-test1 and post-test2, respectively). The sample included a total of 23 subjects. Data were analyzed by SPSS 12.0 program using repeated measure ANOVA. Results: Depression was not statistically significant difference among pre-test, post-test1 and post-test2. Sleep was improved after intervention $37.61{\pm}7.41$(pre-test), $41.22{\pm}8.26$ (post-test1), $44.33{\pm}7.67$(post-test2) (F=8.182. p=.001) Mood was significantly improved after intervention: $16.95{\pm}4.89$(pre-test), $14.89{\pm}6.31$(post-test1), $12.11{\pm}5.04$(post-test2). Smell including smell perception, discomfort due to smell and relation between age and smell was significantly improved after intervention: $2.63{\pm}1.20$ (pre-test). $2.00{\pm}1.16$(post-test1), $1.63{\pm}.96$(post-test2) (F=6.176. p=.006) in smell perception, $2.50{\pm}1.10$ (pre-test). $1.81{\pm}.91$(post-test1), $1.19{\pm}.40$(post-test2) (F=12.26. p=.000) in discomfort due to smell and $3.53{\pm}1.18$(pre-test). $2.59{\pm}1.46$(post-test1), $2.53{\pm}1.33$(post-test2) (F=4.148, p=.025) in relation between age and smell. Conclusion: The results suggest that aroma-inhalation may have an effect on sleep, mood and smell and can be effective nursing intervention to increase the relaxation in stroke patients and their caregivers.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.25 no.2
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• pp.62-67
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• 2015

The carbon microspheres of a core-shell type were grown by the method of mixed-source hydride vapor phase epitaxy (HVPE). The surface and the cross section of the carbon microsphere grown by a new method were observed by scanning electron microscope (SEM). The characteristics of the carbon microsphere were investigated by X-ray photoelectron spectroscopy (XPS) and a high resolution-transmission electron microscope (HR-TEM). From these measurements, the diameters of carbon sphere were about few hundred micrometers. Furthermore, we show that the carbon microsphere of the core-shell type by mixed-source HVPE method can be grown successfully with the larger size than those of the existing one. This mixed-source HVPE method is proposed a new method for making of carbon microsphere.

• Herbal Formula Science
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• v.28 no.1
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• pp.1-13
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• 2020

Objectives : Present study investigated hepatoprotective effects of four types of Taraxaci Herba water extract (TL-F, Taraxaci Herba leaf originated from foreign country; TR-F, Taraxaci Herba root originated from foreign country; TL-K, Taraxaci Herba leaf originated from Korea; TR-K, Taraxaci Herba root originated from Korea) on carbon tetrachloride (CCl₄)-induced acute liver injury. Methods : Mice were administered orally with 200 mg/kg of TL-F, TR-F, TL-K, or TR-K for seven days, and intraperitoneally injected with 0.5 mL/kg of CCl₄ 1 h after last Taraxaci Herba treatment. Silymarin (100 mg/kg) was used as a positive drug. Body weight gain, relative liver weight, serum biochemistry, histopathological and immunohistochemical analyses, and hepatic antioxidant capacity were determined to investigate the hepatoprotective effect of four extracts. Results : Administration of four types of Taraxaci Herba extract increased body weight gain, and decreased relative liver weight in CCl₄-injected mice. As compared to CCl₄ group, TL-F and TR-F significantly decreased serum aspartate aminotransferase activity, while four extracts reduced CCl₄-induced alanine aminotransferase activity. In addition, TL-F and TR-F significantly decreased the numbers of degenerated hepatocytes, infiltrated inflammatory cells, cleaved caspase-3 positive cells, and cleaved PARP positive cells in hepatic tissues. Moreover, TL-F, TR-F, and TR-K administration reduced the lipid peroxidation and nitrotyrosine, and increased glutathione, superoxide dismutase, and catalase activities in hepatic tissues. There were no statistical differences between TL-F- and silymarin-treated group. Conclusion : Of four extracts tested, present results suggest that TL-F is the most favorable candidate against CCl₄-induced acute liver injury through enhancing antioxidant activity.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.6
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• pp.41-55
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• 2011

This study was carried out to survey and analyze the transition of landscape plants on an apartment complexes in the 1990's and 2000's. The results are summarized as follows; The ratio of landscape area was increased from 30.0% to 34.7%. The number of trees per 100 square meter decreased from 22.5 to 15.7. The number of shrub per 10 square meter increased from 19.3 to 38.7. Species of Evergreen tree were increased 21 to 39. The species that a lot of use continuously were Pinus densiflora, Pinus strobus and Taxus cuspidata. The species that increase use were Abies holophylla and Pinus densiflora for. multicaulis. The species that decrease use exceedingly, were Pinus parviflora, Juniperus chinensis, Pinus koraiensis and Thuja orientalis. The species that do not use were Cedrus deodara. Species of deciduous tree were increased 42 to 68. The species that a lot of use continuously were Zelkova serrata, Acer palmatum, Diospyros kaki, Prunus armeniaca and Prunus yedoensis. The species that increase use were Lagerstroemia indica, Chionanthus retusa, Cornus officinalis, Styrax japonica, Sorbus alnifolia, Prunus mume, Cercidiphyllum japonicum, Acer triflorum, Prunus sargentii, Chaenomeles sinensis and Cornus kousa. The species that decrease use exceedingly, were Acer buergerianum, Sophora japonica, Malus spp., Ginkgo biloba, Zizyphus jujuba var. inermis, Platanus orientalis and Albizzia julibrissin. The species that do not use were Liriodendron tulipifera, Acer saccharinum, Ailanthus altissima and Paulownia coreana. Species of shrub were incresed 39 to 65. The species that a lot of use continuously were Buxus koreana, Rhododendron schlippenbachii for. albiflorum, Rhododendron schlippenbachii, Syringa dilatata and Euonymus japonica. The species that increase use were Rhododendron indicum, Ilex serrata, Spiraea prunifolia var. simpliciflora, Taxus cuspidata var. nana, Kerria japonica, Rhododendron yedoense var. poukhanense, Euonymus alatus, Sorbaria sorbifolia var. stellipila, Nandina domestica, Cornus alba, Hydrangea serrata for. acuminata, Prunus tomentosa, Deutzia parviflora and Pyracantha angustifolia. The species that decrease use exceedingly, were Hibiscus syriacus, Rosa multiflora var. platyphylla, Chaenomeles lagenaria and Rosa spp. The species that do not use were Juniperus chinensis var. sargentii, Jasminum nudiflorum and Sasa borealis.

• Journal of Family Resource Management and Policy Review
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• v.17 no.2
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• pp.55-74
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• 2013

This research developed standardized satellite accounts for household production to measure the entire production in Korea using representative Korean data. This study utilized standardized methods for measuring the expenditure and consumption of expendable supplies as an element of intermediate goods in household production. The results of this research can be summarized as follows. First, the total value of household production in Korea in 2009 turned was 268 trillion won, which would be 10.16% of the GDP, based on using the method of replacement by specialty for estimating the value of housework, while it was 356 trillion won, which would be 13.5% of the GDP (2,636 trillion won), based on the method of opportunity cost. Second, the economic value of labor invested in household production was 220 trillion won when using the data from 12 main corresponding wages in the market, while it was 203 trillion won when using the method of total replacement, which used the wages of housekeepers, and 290 trillion won when using the method of opportunity cost in measuring the value of housework labor. Third, in estimating the proportion by section in terms of the total value of household production, the portion of labor in household production was measured from 76% to 82%, depending on the methods used, which is the most significant section. These results indicate that housework has the characteristics of labor-centered work. On the other hand, the value of expenditure on and consumption of expendable supplies, as one element of intermediate goods, is 63 trillion won, which is 17.7% (using the method of opportunity cost in measuring the labor of housework) of household production. Also, the value of capital goods, as the other element of intermediate goods is 2.3 trillion won, which is 0.9% (using the method of opportunity cost) of household production. In conclusion, as an element of household production, housework labor was higher in household production than expenditures on expendables, especially for cooking, cleaning, and caring for children. In the future, people should be cautious about estimating that the amount of household work could be reduced, as women's labor in the market and the household work produced by the market will increase.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.4
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• pp.303-309
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• 1990

To provide essential information of the role of marine bacteria on the dinoflagellate blooms, distribution of marine bacterial flora and dinoflagellate species was investigated in Chinhae Bay located in southern part of Korea from August 1989 to April 1990. Two hundred and fifty one strains of marine bacteria were isolated from seawater samples collected from the study area. Among them, Flavobacterium spp. and Acinetobacter spp. were the most dominant in bacterial flora. Another 32 strains which comprised 13 percent of total strains were Erythrobacter spp.. Based on the physiological character, Erythrobacter spp. were identified as Erythrobacter longus, Erythrobacter sp.(J-2) and Erythrobacter sp. (J-8). From the phytoplanktonic community, fourteen genera and twenty nine taxa of dinoflagellate species were identified. Based on the spatio-temporal frequence and abundance Gymnodinium sanguneum, Prorocentrum micans and Prorocentrum minimum were the aestival dominent species. However, Heterocapsa triquetra was appeared as predominant species in April. Cell density of about 2,000 cells/ml was prevailed in the bloom of August, but it developed into more intensive bloom of above 500 cells/ml in September. The water quality showed eutrophic or hypereutrophic condition, which was proved by high concentration of dissolved inorganic nitrogen, ortho-phosphate and chemical oxygen demand. Oxygen deficient water mass was found in the bottom overlying waters in August and September. High relationship between abundant bacterial flora and persistent dinoflagellate blooms in eutrophic condition would be approvable.

• Journal of Environmental Science International
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• v.23 no.10
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• pp.1703-1718
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• 2014

An eco-friendly integrated multi-trophic aquaculture (IMTA) farming technique was developed with the goal of resolving eutrophication by excess feed and feces as fish-farming by-products. A variety of seaweed species were tried to remove inorganic nutrients produced by fish farming. However, there have been few trials to use Sargassum fulvellum in an IMTA system, a species with a relatively wide distribution across regions with various habitat conditions, great nutrient removal efficiency and importance for human food source and industrial purposes. In this regard, our study tried to examine feasibility of using S. fulvellum in an IMTA system by analyzing growth characteristics of the species in an IMTA system comprising of rockfish (Sebastes shlegeli), sea cucumber (Stichopus japonocus) and the tried S. fulvellum (October 2011 - November 2012). We also monitored environment conditions around the system including current speed, water temperature and inorganic nutrient level as they may affect growth of S. fulvellum. S. fulvellum in the IMTA system, which were $15.72{\pm}5.67mm$ long at the start of the experiment in October 2011, grew to a maximum of $1093{\pm}271.13mm$ by May 2012. In September, seaweed growth was reduced to a minimum of $280{\pm}70.43mm$ in length. Then, S. fulvellum began to grow again reaching $325{\pm}196.19mm$ by November 2012. Wet weight of the seaweed was $4.01{\pm}1.89g$ at the start of the experiment and reached a maximum of $109.26{\pm}34.23g$ in May. The weight gradually declined to a low of $15.12{\pm}8.40g$ in September 2012. Weight began to increase once more, rising to $39.27{\pm}21.69g$ by November. During the experiment, the average velocity at the surface and the bottom was 6.5 cm/s and 3.4 cm/s, respectively. The water temperature ranged $5.0-23.5^{\circ}C$, which was considered suitable for growing S. fulvellum. Results of the study indicated no significant differences in inorganic nutrients between pre- and post-IMTA installation. It was thus concluded that S. fulvellum can be a suitable seaweed species to be used in an IMTA system.