• Title/Summary/Keyword: Science and Technology (S&T)

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Purification of Materials Produced by Amylocolatosis sp. and Anticancer Effect in Oral Cancer Model (Amylocolatosis sp.가 생산하는 항암물질의 정제 및 구강암 모델에 미치는 항암 효과)

  • Kim, Jung;Park, Young-Min;Lim, Do-Seon
    • Journal of dental hygiene science
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    • v.3 no.1
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    • pp.11-14
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    • 2003
  • A methylotrophic Actinomycetes strain, which produce the anti-oral cancer activity compound, was isolated from soil and estimated as Amylocolatosis sp. based on taxonomic studies. A methanol didn't have influence on the production of the anticancer compounds. These compound were isolated by ethylacetate extract, silica gel column chromatography, sephadex LH-20 column and reverse phase HPLC. The compounds were very stable under heat ($121^{\circ}C$), acid(pH 2.0) and alkali(pH 11.0) treatment. The cytotoxic effect of isolated anticancer compounds on various cancer cell lines such as A549, SNU-1, KB, L1210, and Sarcoma 180 was investigated by MTT assay method. And these produced compounds also showed the broad antimicrobial spectrum to test strains such as bacteria and yeast.

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Characterization of a Potential Probiotic Lactiplantibacillus plantarum LRCC5310 by Comparative Genomic Analysis and its Vitamin B6 Production Ability

  • Yunjeong Lee;Nattira Jaikwang;Seong keun Kim;Jiseon Jeong;Ampaitip Sukhoom;Jong-Hwa Kim;Wonyong Kim
    • Journal of Microbiology and Biotechnology
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    • v.33 no.5
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    • pp.644-655
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    • 2023
  • Safety assessment and functional analysis of probiotic candidates are important for their industrial applications. Lactiplantibacillus plantarum is one of the most widely recognized probiotic strains. In this study we aimed to determine the functional genes of L. plantarum LRCC5310, isolated from kimchi, using next-generation, whole-genome sequencing analysis. Genes were annotated using the Rapid Annotations using Subsystems Technology (RAST) server and the National Center for Biotechnology Information (NCBI) pipelines to establish the strain's probiotic potential. Phylogenetic analysis of L. plantarum LRCC5310 and related strains showed that LRCC5310 belonged to L. plantarum. However, comparative analysis revealed genetic differences between L. plantarum strains. Carbon metabolic pathway analysis based on the Kyoto Encyclopedia of Genes and Genomes database showed that L. plantarum LRCC5310 is a homofermentative bacterium. Furthermore, gene annotation results indicated that the L. plantarum LRCC5310 genome encodes an almost complete vitamin B6 biosynthetic pathway. Among five L. plantarum strains, including L. plantarum ATCC 14917T , L. plantarum LRCC5310 detected the highest concentration of pyridoxal 5'-phosphate with 88.08 ± 0.67 nM in MRS broth. These results indicated that L. plantarum LRCC5310 could be used as a functional probiotic for vitamin B6 supplementation.

Radon Hazard Review of Spilled Groundwater and Tap Water in Incheon Metropolitan City Subway Station (인천광역시 지하철 역사 내 지하수 및 수돗물의 라돈 위해성 검토)

  • Lee, Yoo-Sang;Lee, Sang-Bok;Kang, Min-Seok;Jeong, Dong-Ha;Kim, Jin-Hong;Oh, Yoon-Sik;Choi, Se-Rin;Park, Jeong-Soo;Kim, Sungchul
    • Journal of radiological science and technology
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    • v.44 no.6
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    • pp.671-677
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    • 2021
  • Interest in the everyday hazards of radon has recently increased as such, this study attempted to examine the dangers of radon in spilled groundwater by comparing the radon concentrations of the drained groundwater and tap water used in recirculating systems in Incheon Subway restrooms. At five stations of Incheon Subway Line 1 and three stations of Line 2, drained groundwater is recirculated and used in restrooms for toilet flushing. Stations restroom tap water for hand washing that used as a control and the measured values of each were compared. With the cooperation of Incheon Transportation Corporation, samples of spilled groundwater and tap water were collected sealed to prevent contact with the air, and a DURRIDGE RAD7 was used as the experimental equipment. The collected samples were subjected to radial equilibration for approximately 3.5 h, at which the radon concentration reached its maximum, and then calculated as 10 measurements using the RAD7 underwater radon measurement mode. In all eight stations, the radon concentration in tap water was lower than the recommended amount. However, in an average of 7 out of the eight stations, the radon concentration in the effluent groundwater was 100 times higher than that in tap water. Since high radon concentrations in groundwater runoff can be harmful to humans, and there is no accurate standard for radon concentrations in domestic water, it is necessary to continuously monitor radon in water and prepare a guidance of recommended values.

Suggestions for Color Palette Interface - focusing on Adobe's illustration - (컬러팔레트인터페이스에 관한 제안 -어도비사의 일러스트레이션을 중심으로-)

  • Choi, Young-Ok
    • Archives of design research
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    • v.20 no.3 s.71
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    • pp.15-28
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    • 2007
  • It is safe to say that color is among the most important elements in design. Thus, most designers give much thought about color while finishing their works. However, it is not easy to use the color interface of computer graphics programs, which makes it difficult for designers to apply the color theory and color scheme theory they've learned at school into the real design works. In fact, the same color systems as those of Adobe's first version of Photoshop or other illustration programs are still being used. Despite the advancement of digital equipment and development of printing technology, color palettes haven't changed accordingly, making it difficult to find a proper color coordination. Based on surveys and color scheme theories and taking designers' convenience into consideration, I will suggest in this paper a new color interface with which the color system and color scheme theories can be easily applied. However, its use in reality is not dealth with in this paper since it requires more practical discussions and studies.

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Allochthonous Organic Matter Contribution to Foodweb in Shingu Agricultural Researvoir after Rainfall Period (강우기 후 신구 농업용 저수지 먹이망에 미치는 외부기원 유기물의 영향 - 안정동위원소비 활용 -)

  • Kim, Min-Seob;Lee, Yeon-Jung;An, Kwang-Guk;Kim, Baik-Ho;Hwang, Soon-Jin;Shin, Kyung-Hoon
    • Korean Journal of Ecology and Environment
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    • v.47 no.1
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    • pp.53-61
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    • 2014
  • The origin of particulate organic matter (POM) and food web structure were investigated in Shingu reservoir based on stable isotope analysis from pre-monsoon (July) to post-monsoon (September) 2007. According to the depth in Shingu reservoir, the $^{13}C$ and $^{15}N$ values of POM for pre-monsoon period were nonsignificant distinction, while it was significant variation after rainfall period. The ${\delta}^{13}C$ values of POM in premonsoon period ranged from -25.1‰ to -26.1‰ in whole water column, but the ${\delta}^{13}C$ values of POM in post-monsoon period showed relatively wide range between -23.2‰ and -27.5‰. The apparently lighter values (average -27.5‰) in near bottom water (7 m water depth) demonstrate that POM in high turbid water in post-monsoon period may be derived from the outside terrestrial plants (allochthonous) through heavy rainfall during the summer monsoon period. After rainfall period, $^{13}C$ and $^{15}N$ values of D. brachyurum showed -23.3‰ and 12.2‰, respectively, while B. longirostris showed -27.1% and 8.7%, respectively. It suggested that D. brachyurum mainly feed on POM in autochthonous organic matter pool, but B. longirostris mainly consumed POM in allochthonous organic matter pool after rainfall period. Carbon and nitrogen stable isotope ratios were markedly different among secondary consumers. The carp (C. carpio) and catfish (S. asotus) were in the higher trophic level and crucian carp (C. auratus) and mudfish (M. mizolepis) were in the lower trophic level. $^{13}C$ and $^{15}N$ values of Z. platypus didn't significantly changed between before and after rainfall period. But P. parva and C. auratus apparently changed the $^{13}C$ and $^{15}N$ values after rainfall period. It is suggested that P. parva and C. auratus seem to feed allochthonous food source while Z. platypus depend on autochthonous food source.

Characteristics of Manure and Estimation of Nutrient and Pollutant of Holstein Dairy Cattle (홀스타인 젖소 분뇨의 특성과 비료성분 및 오염물질 부하량 추정)

  • Choi, D.Y.;Choi, H.L.;Kwag, J.H.;Kim, J.H.;Choi, H.C.;Kwon, D.J.;Kang, H.S.;Yang, C.B.;Ahn, H.K.
    • Journal of Animal Science and Technology
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    • v.49 no.1
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    • pp.137-146
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    • 2007
  • This study was conducted to determine fertilizer nutrient and pollutant production of Holstein dairy cattle by estimating manure characteristics. The moisture content of feces was 83.9% and 95.1% for urine. The pH of feces and urine were in the ranges of 7.0~7.4 and 7.5~7.8, respectively. The average BOD5, COD, SS, T-N, T-P concentrations of the dairy feces were 18,294, 52,765, 102,889, 2,575, and 457mg/ℓ, respectively. Dairy urine showed lower levels of BOD5(5,455mg/ℓ), COD(8,089mg/ℓ), SS(593mg/ℓ), T-N(3,401mg/l), and T-P(13mg/ℓ) than feces. The total daily produced pollutant amounts of a dairy cow were 924.1g(Milking cow), 538.8g(Dry cow), 284.4g(Heifer) of BOD5, 2,336.5g (Milking cow), 1,651.8g(Dry cow), 734.1g(Heifer) of COD and 4,210.1g(Milking cow), 2,417.1g(Dry cow), 1,629.1g(Heifer) of SS and 194.8g(Milking cow), 96.4g(Dry cow), 58.3g(Heifer) of T-N and 24.0g(Milking cow), 10.2g(Dry cow), 6.1g(Heifer) of T-P. The calculated amount of pollutants produced by a 450kg dairy cow for one year were 181.3kg of BOD5, 492.5kg of COD, 899.9kg of SS, 36.0kg of T-N and 4.1kg of T-P. The total yearly estimated pollutant production from all head(497,261) of dairy cattle in Korea is 90,149 tons of BOD5, 244,890 tons of COD, 447,491 tons of SS, 17,898 tons of T-N and 2,008 tons of T-P. The fertilizer nutrient concentrations of dairy feces was 0.26% N, 0.1% P2O5 and 0.14% K2O. Urine was found to contain 0.34% N, 0.003% of P2O5 and 0.31% K2O. The total daily fertilizer nutrients produced by dairy cattle were 197.4g (Milking cow), 97.4g(Dry cow), and 57.9g(Heifer) of Nitrogen, 54.2g(Milking cow), 22.2g(Dry cow), and 14.2g(Heifer) of P2O5 and 110.8g(Milking cow), 80.4g (Dry cow), and 39.5g(Heifer) of K2O. The total yearly estimated fertilizer nutrient produced by a 450kg dairy animal is 36.2kg of N, 8.8kg of P2O5, 24.6kg of K2O. The estimated yearly fertilizer nutrient production from all dairy cattle in Korea is 18,000 tons of N, 4,397 tons of P2O5, 12,206 tons of K2O. Dairy manure contains useful trace minerals for crops, such as CaO and MgO, which are contained in similar levels to commercial compost being sold in the domestic market. Concentrations of harmful trace minerals, such as As, Cd, Hg, Pb, Cr, Cu, Ni, Zn, met the Korea compost standard regulations, with some of these minerals being in undetected amounts.

The Prevalence of Low Back Pain Disability Index in College Students (대학생의 요통장애지수에 대한 분포)

  • Kwon, Won-An;Kim, Dong-Dae;Um, Ki-Mae;Song, Young-Hwa;Oh, Hyun-Ju;Lee, Jae-Hong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.10
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    • pp.3846-3852
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    • 2010
  • Low back pain is commonly experienced by both adolescents and adults. The aim of this study is to research the prevalence of the Oswestry disability index(ODI) in their 20s of college students. The data was collected from the ODI questionnaire on functional disability measures for college students(males: 233, females: 407). Statistical analysis was carried out with SPSS 12.0 program, which was used for descriptive statistics and Pearson correlation analysis. The results were : Firstly, 'sitting' item was showed 'the highest score' and 'the lowest score' was 'personal care' in ODI items. Secondly, there was statistically significant between female(6.13) and male(4.62) on ODI but not ODI grade(p<0.01). Thirdly, there was a statistically significant relationship between ODI grade and each ODI items(p<0.01). These results have specific meaning as future clinical and epidemiological studies.

A comparison with Result of Normalized image to Different Template image on Statistical Parametric Mapping of ADHD children patients (과잉행동장애 어린이의 SPM(Statistical Parametric Mapping)분석에서 서로 다른 Template image로 규격화된 SPEC 영상의 결과 비교)

  • Shin, Dong-Ho;Park, Soung-Ock;Kwon, Soo-Il;Joh, Chol-Woo;Yoon, Seok-Nam
    • Journal of radiological science and technology
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    • v.26 no.2
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    • pp.71-78
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    • 2003
  • We studied 64 ADHD children patients group($4{\sim}15\;ys$, mean age : $8{\pm}2.6\;ys$. M/F:52/12) and 12 normal group($6{\sim}7\;ys$, mean age : $9.4{\pm}3.4\;ys$, M/F:8/4) of the brain has been used to analysis of blood flow between normal and ADHD group. For analysis of Children ADHD, we used 12 children's mean brain images and made Template image of SPM99 program. In increase of blood flow(P-value 0.05), the result of normalized images to Template image to offer from SPM99 program, showed significant cluster in inter-Hemispheric and occipital Lobe, in the case of normalized images to children template image, showed inter-hemispheric and parietal lobe.

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Design and Implementation of a Small Server Room Environment Monitoring System by Using the Arduino (아두이노를 이용한 소규모 서버 룸 환경 모니터링 시스템의 설계 및 구현)

  • Lee, Hyo-Seung;Oh, Jae-Chul
    • The Journal of the Korea institute of electronic communication sciences
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    • v.12 no.2
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    • pp.385-390
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    • 2017
  • Owing to the development of IT technology, a computerized system in various ways such as a variety of company's businesses, factory automation system and hospital healthcare system is introduced and operated. And it is possible to say that a computer system is more important than anything else to the extent that all businesses are suspended in case the system is down. Attention should be always paid to environmental management such as temperature, humidity and fire in server room for the normal operation of system in this situation. It is thought that there is necessity for a low-cost system which independently monitors environment round the clock in the situation where the person in charge doesn't pay attention and which informs a person in charge in real time when an event occurs for the operation of this small server room. Consequently, it is to be hoped that the suspension of service provided by computer system, which may occur due to a specific event, can be prevented.

Survey on the Sulfonamide Residues in Beef, Pork and Chicken (HPLC법에 의한 식육중의 설파제 잔류량 조사)

  • Park, J.T.;Jeong, E.J.;Kim, Y.G.;Song, B.J.;Oh, K.S.;Lim, H.C.;Kim, S.C.
    • Korean Journal of Food Science and Technology
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    • v.26 no.3
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    • pp.221-225
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    • 1994
  • This survey was carried out to determine five sulfonamide(sulfamerazine, sulfamethazine, sulfadimethoxine, sulfamonomethoxine, sulfaquinoxaline) residues in beef, pork, chicken and swine kidney. For this survey, 30 samples of beef, 15 samples of chicken, 10 samples of pork and 10 samples of swine kidney were collected in Chonnam from June, 1992 to June, 1993, and were analyzed by HPLC. The recoveries of sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfadimethoxine, and sulfaquinoxaline in spiked samples between $0.25{\sim}1.00$ ppm were 71.7%, 80.3%, 71.6%, 70.9%, 68.4%, respectively. None of 65 samples which were examined exceeded 0.1 ppm. Of 15 chicken muscle samples, 2 samples exceeded 0.05 ppm in sulfamerazine (0.077 ppm) and sulfamethazine (0.075 ppm), respectively. Of 10 swine kidney samples, 1 sample exceeded 0.05 ppm in sulfadimethoxine (0.052 ppm). And sulfanilamide concentration of swine kidney were higher than pork.

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