• 융합정보논문지
• /
• 제10권4호
• /
• pp.81-88
• /
• 2020

본 연구의 목적은 유아교사의 직무스트레스와 자아탄력성이 삶의 만족도에 미치는 영향을 확인하고 삶의 만족도의 질적 향상 방안을 탐색하는 것이다. 이를 위해 강원도에 소재한 공·사립 유치원, 어린이집 교사 198명을 대상으로 직무스트레스, 자아탄력성, 삶의 만족도 설문지를 사용하여 응답하게 하였다. 수집된 자료를 토대로 Pearson의 적률상관관계 분석과 위계적 중다회귀분석을 실시한 결과는 다음과 같다. 첫째, 유아교사의 직무스트레스가 높을수록 삶의 만족도는 낮아지며, 자아탄력성이 높을수록 삶의 만족도가 높아지는 유의미한 관계가 나타났다. 특히 직무스트레스의 하위변인인 경제적 영역은 유아교사의 자아탄력성, 삶의 만족도와 유의미한 부적상관이 나타났다. 둘째, 경제적 영역과 관계탄력성, 활동성의 예측변인들이 유아교사의 삶의 만족도에 유의하게 영향을 미치며, 자아탄력성의 하위변인인 관계탄력성이 삶의 만족도에 대해 상대적으로 설명력이 가장 크게 나타났다. 이는 교사의 삶의 만족도를 높이기 위해 자아탄력성을 촉진시킬 프로그램 개발이 필요함을 시사한다.

• 한국생명과학회:학술대회논문집
• /
• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
• /
• pp.84.1-84.1
• /
• 2007

See Full Text

• Asian-Australasian Journal of Animal Sciences
• /
• 제32권8호
• /
• pp.1095-1103
• /
• 2019

Objective: Among stress responses, the unfolded protein response (UPR) is a well-known mechanism related to endoplasmic reticulum (ER) stress. ER stress is induced by a variety of external and environmental factors such as starvation, ischemia, hypoxia, oxidative stress, and heat stress. Inositol requiring enzyme $1{\alpha}$ ($IRE1{\alpha}$)-X-box protein 1 (XBP1) is the most conserved pathway involved in the UPR and is the main component that mediates $IRE1{\alpha}$ signalling to downstream ER-associated degradation (ERAD)- or UPR-related genes. XBP1 is a transcription factor synthesised via a novel mechanism called 'frame switch splicing', and this process has not yet been studied in the horse XBP1 gene. Therefore, the aim of this study was to confirm the frame switch splicing of horse XBP1 and characterise its dynamics using Thoroughbred muscle cells exposed to heat stress. Methods: Primary horse muscle cells were used to investigate heat stress-induced frame switch splicing of horse XBP1. Frame switch splicing was confirmed by sequencing analysis. XBP1 amino acid sequences and promoter sequences of various species were aligned to confirm the sequence homology and to find conserved cis-acting elements, respectively. The expression of the potential XBP1 downstream genes were analysed by quantitative real-time polymerase chain reaction. Results: We confirmed that splicing of horse XBP1 mRNA was affected by the duration of thermal stress. Twenty-six nucleotides in the mRNA of XBP1 were deleted after heat stress. The protein sequence and the cis-regulatory elements on the promoter of horse XBP1 are highly conserved among the mammals. Induction of putative downstream genes of horse XBP1 was dependent on the duration of heat stress. We confirmed that both the mechanisms of XBP1 frame switch splicing and various binding elements found in downstream gene promoters are highly evolutionarily conserved. Conclusion: The frame switch splicing of horse XBP1 and its dynamics were highly conserved among species. These results facilitate studies of ER-stress in horse.

• 한국학교ㆍ지역보건교육학회지
• /
• 제15권3호
• /
• pp.31-41
• /
• 2014

Objective: The purpose of this study was to obtain the basic data for quality of life and improve the oral health through relevance of academic stress with academic high school girl temporomandibular joint syndrome. Methods: Data was collected from the students of two academic girl high school in Daegu, using questionnaire. They were the students of the 1st, 2nd grade, in total 311 student. The analysis was made using ${\chi}^2-test$, (one way ANOVA), and Pearson's Correlation, multiple linear regression analysis. These were conducted using spss 14.0 version Results: High school girl had at least one temporomandibular joint syndrome was 70.4%. In subjective of temporomandibular joint syndrome academic stress for grade stress scores were highest 52.3, study stress 35.0 all the depth of a lesson stress 45.5, there was a statistically significant(p<0.001). effect on subjective of temporomandibular joint syndrome influence the bad oral habit(${\beta}=0.325$) were found to have subjective of joint syndrome showed a statistically significant increase in the higher significant positive(p<0.001).

• 대한간호학회지
• /
• 제37권4호
• /
• pp.549-557
• /
• 2007

Purpose: The purpose of this study was to identify the relationship among job stress, family stress and perceived health status of middle school teachers and to present basic information about promoting health and coping with stress. Method: Participants(N=547) was recruited in B city from November 2005 to December 2005. Data were analyzed with descriptive statistics, t-test, ANOVA, Pearson's correlation and multiple regression. Results: The degree of Job stress of the middle school teachers was 54.47 out of a total score of 88; that of family stress was 46.57 out of a total score of 96; and that of perceived health status was 78.59 out of the perfect score 100. There was a significantly negative relationship between job stress and perceived health status (r=-.274, p<.001), and family stress and perceived health status(r=-.408, p<.001). However, there was a positive relationship between job stress and family stress(r=.298, p<.001).Family stress, gender, charging subject, job stress, charging grade and number of family member was 27.1% of the variance in perceived health status of middle school teachers. Conclusion: Family stress has the most important impact on perceived health status with middle school teachers. Based on the finding, we could conclude that both job stress and family stress management should be required to improve perceived health status.

• 대한한방부인과학회지
• /
• 제27권3호
• /
• pp.1-11
• /
• 2014

Objectives: Hwangryunhaedok-tang (HH) is a representative heat clearing multiherb prescription. We evaluated the protective effect of HH against heat stress exposure in mice. Methods: Six weeks old ICR mice were used for this study. After $43^{\circ}C$ heat stress for 15 minutes, we evaluated the changes of motor activity and c-fos expression level to determine the proper heat stress and evaluation time. The subjects were divided into 4 groups (1. control group, 2. heat stress group with normal saline, 3. heat stress group with administration of 100 mg/kg, and 4. heat stress group with administration of 1000 mg/kg). After oral administration of HH once a day for 3 days, 2, 3, 4 groups were exposed to $43^{\circ}C$ heat stress for 15 minutes. Then, we evaluated the motor activity for 120 minutes and analyzed the c-fos expression using western blot. We investigated the effect of HH and its ingredients on c-fos expressions after heat stress. The mice were administrated HH, Scutellariae Radix, Coptidis Rhizoma, Phellodendri Cortex, and Gardeniae Fructus for 3 days. After one hour of last treatment, the mice were exposed to heat stress at $43^{\circ}C$ for 15 min. After two hours, the hypothalamus was dissociated and lysed to measure c-fos expression. Results: After oral administration of HH for 3 days, motor activity was recovered insignificantly. 100 mg/kg HH treatment reduced the c-fos expression after heat stress but insignificantly. Among the ingredients of HH, Coptidis Rhizoma and Scutellariae Radix treatment groups significantly reduced the c-fos after stress. Conclusions: These results show that Hwangryunhaedok-tang may be effective to reduce the heat stress response.

• Animal Bioscience
• /
• 제34권1호
• /
• pp.134-142
• /
• 2021

Objective: To understand the athletic characteristics of Thoroughbreds, high-throughput analysis has been conducted using horse muscle tissue. However, an in vitro system has been lacking for studying and validating genes from in silico data. The aim of this study is to validate genes from differentially expressed genes (DEGs) of our previous RNA-sequencing data in vitro. Also, we investigated the effects of exercise-induced stress including heat, oxidative, hypoxic and cortisol stress on horse skeletal muscle derived cells with the top six upregulated genes of DEGs. Methods: Enriched pathway analysis was conducted using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) tool with upregulated genes in horse skeletal muscle tissue after exercise. Among the candidates, the top six genes were analysed through geneMANIA to investigate gene networks. Muscle cells derived from neonatal horse skeletal tissue were maintained and subjected to exercise-related stressors. Transcriptional changes in the top six genes followed by stressors were investigated using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: The inflammation response pathway was the most commonly upregulated pathway after horse exercise. Under non-cytotoxic conditions of exercise-related stressors, the transcriptional response of the top six genes was different among types of stress. Oxidative stress yielded the most similar expression pattern to DEGs. Conclusion: Our results indicate that transcriptional change after horse exercise in skeletal muscle tissue strongly relates to stress response. The qRT-PCR results showed that stressors contribute differently to the transcriptional regulation. These results would be valuable information to understand horse exercise in the stress aspect.

• Journal of Microbiology and Biotechnology
• /
• 제27권10호
• /
• pp.1790-1797
• /
• 2017

In the present study, we demonstrate that the growth of salt-stressed pepper plants is improved by inoculation with plant growth-promoting rhizobacteria (PGPR). Three PGPR strains (Microbacterium oleivorans KNUC7074, Brevibacterium iodinum KNUC7183, and Rhizobium massiliae KNUC7586) were isolated from the rhizosphere of pepper plants growing in saline soil, and pepper plants inoculated with these PGPR strains exhibited significantly greater plant height, fresh weight, dry weight, and total chlorophyll content than non-inoculated plants. In addition, salt-stressed pepper plants that were inoculated with B. iodinum KNUC7183 and R. massiliae KNUC7586 possessed significantly different total soluble sugar and proline contents from non-inoculated controls, and the activity of several antioxidant enzymes (ascorbate peroxidase, guaiacol peroxidase, and catalase) was also elevated in PGPR-treated plants under salt stress. Overall, these results suggest that the inoculation of pepper plants with M. oleivorans KNUC7074, B. iodinum KNUC7183, and R. massiliae KNUC7586 can alleviate the harmful effects of salt stress on plant growth.

• 한국해양공학회지
• /
• 제25권6호
• /
• pp.49-55
• /
• 2011

In order to analyze feasibility of replacing a conventional 6-mm Ti bar with a 5-mm bar, a series of rotating bending fatigue tests were carried out on Ti-6Al-4V bars by strengthening the fatigue performance using a special technique called UNSM (Ultrasonic Nanocrystal Surface Modification). The results of S-N curves clearly showed that the performance of the 5-mm titanium specimen was similar to that of the 6-mm specimen when the UNSM treatment was applied. The 5-mm treated specimen converged with small scattering band into the linear line of the non-treated 6-mm one. Below the fatigue life of $10^5$ cycles, the UNSM treatment did not show any significant superiority in the bending stress and fatigue life. However, over the fatigue life of $10^5$ cycles, the effect of UNSM was superior for each fatigue life, and the bending stress became longer and higher than that of the untreated one. In the case of 6-mm Ti-bar with UNSM, the fatigue limit was about 592 MPa, and there was fatigue strength increase of about 30.7% at the fatigue life of $10^4$ cycles compared to the untreated 6-mm bar. Therefore, the compressive residual stress made by the UNSM in Ti-6Al-4V increased the fatigue strength by more than 30%.

• 한국가정과교육학회지
• /
• 제28권4호
• /
• pp.123-137
• /
• 2016

본 연구의 목적은 중 고등학생 자녀를 둔 학부모들이 인지하는 자녀생활역량을 파악하고, 양육스트레스, 부모효능감, 우울감, 부모 교육참여가 자녀생활역량에 어떠한 영향을 미치는지 알아보고자 하는 것이다. 이를 알아보고자 대전광역시에 거주하고 있는 중 고등학교에 재학 중인 자녀를 가진 학부모를 대상으로 하였다. 2013년 4월 기준, 대전광역시 소재 중학교 및 고등학교에 협조공문을 보내, 설문지를 이메일로 전달하였고, 2013년 12월 10일부터 20일 사이 회수된 설문지 중, 결측치가 있는 자료를 분석에서 제외하여 총 800부를 최종 분석에 사용하였다. 분석을 위해 SPSS 23.0 및 AMOS 23.0 프로그램을 이용하여 연구대상자의 일반적 배경 및 백분율을 실시하였다. 변인들의 관계를 파악하기 위해 Pearson 적률상관계수를 사용하였고, 변인들의 경로를 파악하기 위한 경로모형분석과 경로의 직 간접효과를 분석하고 그 유의성을 검증하였다. 연구결과, 자녀생활역량 및 관련 변인들의 높은 상관을 파악할 수 있었으며, 경로모형분석 결과, 부모의 양육스트레스, 부모효능감, 우울감, 부모교육참여가 자녀생활역량에 직 간접적 영향을 미치는 것으로 파악되었다. 부모의 양육스트레스는 학부모효능감과 우울감에 영향을 미치나, 부모교육참여에 따라 자녀생활역량에 다른 영향을 미치는 것을 알 수 있었다. 이와 같은 결과를 바탕으로 자녀생활 역량을 증가시키기 위한 가정교육학계에서의 정책적 대안에 대해 제안하였다.