• Journal of Nutrition and Health
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• v.40 no.2
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• pp.111-117
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• 2007

This study was designed to investigate the effects of pyroligneous liquor on oxygen radicals and their scavenger enzymes in the liver of Cri/Bgi CD rats (7 rats per group). Male rats were fed a basic diet prepared in our Lab., PL-0 (Control), PL-1, PL-25, PL-50 and PL-75 groups were Prepared to be 0%, 1%, 25%, 50% and 75%with distilled water using pyroligneous liquor (35% of Choa Co. Ltd.), and were administrated orally for 8 weeks. Superoxide radical contents in liver mitochondria and microsomes were significantly decreased to 12-14%, 11-15%, respectively, in these PL-25 and PL-50groups compared with the control group. Hydroxyl radical content in mitochondria and microsomes were markedly decreased to 12-20% and 17%, respectively, in these PL-25 and PL-50% groups compared with the control group. Hydrogen peroxide content in mitochondria and microsomes were significantly decreased about 15-12% and 22-20% in liver of PL-25 and PL-50 groups compared with the control group. Mn-SOD and Cu/Zn-SOD activities in liver of PL-25 and PL-50 groups were remarkably increased to 15-25%, 11-16%, respectively, compared with the control group. GPx activities in mitochondria and microsomes were significantly increased in the liver of PL-25 and PL-50 groups compared with the control group. CAT activities in mitochondria and cytosol were significantly increased to 12-14%, 15-27%, respectively, in the liver of PL-25 and PL-50 groups compared with the control group. These results suggest that long term administration orally of 25 and 50% pyroligneous liquor may effectively inhibit the formation of oxygen free radicals, and also scavenger enzyme activities significantly increase through the administration orally.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.2
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• pp.87-92
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• 2000

This study was designed to investigate the effects og $10{\%},\;20{\%}\;and\;40{\%}$-addition of functional brown algae (FBA)-noodles on oxygen radicals and their scavenger enzymes in liver of Sprague-Dawley(SD) male rats. Hydroxyl radicals$({\cdot}OH)$ formations were significantly inhibited$(20{\~}35{\%}\;and\;12{\~}20{\%})$ in liver mitochondria and microsomes of rats administered $0{\%},\;20{\%}\;and\;40{\%}$ FBA-noodles compared with that of control group. Significant differences in $H_2O_2$ formations of liver microsome in these FBA-noodles fed groups could not be obtained, but superoxide-radical $(O_2^({\cdot}-))$ formations of liver cytosol resulted in a significant decrease about $10{\%}\;in\;20{\%}\;and\;40{\%}$ FBA-noodles compared with control group. Mn-SOD activities in liver mitochondria were significanlty increased $(10{\~}15{\%})$ in the groups fed $10{\%},\;20{\%}\;and\;40{\%}$ FBA-noodles, while a group administered $40{\%}$ FBA-noodle only resulted In a significant increases $(about 12{\%})$ in Mn-SOD activity of liver microsomes compared with control group. Cu, Zn-SOD activities in liver cytosol were significantly increased $(10{\~}20{\%})\;in\;10{\%},\;20{\%}\;and\;40{\%}$ FEA-noodles compared with control group. Administration of $10{\%},\;20{\%}\;and\;40{\%}$ FBA-noodles resulted in a marked increases$(20{\~}40{\%})$ in liver cytosolic glutathione peroxidase (GSHPx) compared with control group. Significant differences in lipid peroxide (LPO) levels of mitochondria and microsomes in $10{\%}$ FBA-noodle could not be obtained, while LPO levels of $20{\%} and 40{\%}$ FBA-noodles were significantly inhibited about $10{\%}$ in mitochondria and microsomes compared with control group. These results suggest that these FBA-noodles may play a desirable role in attenuating an oxygen radical formations and increasing a scavenger enzymes activity by some brown algae (Undaria pinnatifida) components.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.889-894
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• 1998

This study was designed as a part of efforts to investigate the biochemical pollutant markers for diagnosis of marine pollutions by changes in oxygen radicals and their scavenger enzymes of the flounder (Paralichthys olivaceus) in the South Sea of Korea. Protein contents in brain and muscle of cultured flounder in the South Sea were remarkably lower ($19\~42\%$ and $25\~41\%$, respectively) than those of wild flounder in Pohang of the East Sea as a control group. Lipid Peroxide (LPO) levels in serum of cultured in the South Sea were considerably higher ($5\~33\%$) Elan those of wild flounder in Pohang. Hydroxyl radical formations in serum of cultured flounders in the South Sea were lower ($4\~25\%$) than those of wild flounders in Pohang. Superoxide dismutase (SOD) activities in serum of cultured flounders in South Sea were also significantly lower ($7\~31\%$) than those of wild flounders in Pohang. It is believed that significant decrease of protein contents in brain and muscle, and remarkable increases of LPO in serum of cultured flounders may be used as a biochemical pollutant markers for diagnosis of marine pollutions. These results suggest that near-coastal water as well as neritic water of the South Sea might be affected by pollutant input as well as the Yellow Sea of Korea.

• Journal of Oral Medicine and Pain
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• v.30 no.3
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• pp.329-336
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• 2005

We examined the effect of PQQ, as a scavenger of superoxide, on osteoclast-like cell formation and on mature osteoclast function. To determine whether PQQ scavenges the superoxide, nitroblue tetrazolium (NBT) staining, which is a method to detect superoxide, was performed on HD-11 cells which are a chick myelomonocytic cell line having tartrate-resistant acid phosphatase (TRAP) activity in response to 1,25-dihydroxyvitamin $D_3\;[1,25(OH)_2D_3]$. Histochemical study of TRAP was also performed on HD-11 cells. PQQ inhibited the TRAP-positive multinucleated cell formation of chicken bone marrow cells was also examined. The addition of 20 ${\mu}M$ PQQ inhibited the formation of TRAP-positive multinucleated cell. When chicken osteoclasts were cultured on dentin slices, treatment of 20 ${\mu}M$ PQQ resulted in a significant decrease in dentin resorption by osteoclasts in terms of total resorption area and number of resorption pits. The present data suggest that PQQ, possibly as a scavenger of superoxide ion, inhibits the osteoclastic differentiation and bone resorption.

• Development and Reproduction
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• v.12 no.2
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• pp.125-132
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• 2008

Melatonin (N-acetyl-5-methoxytryptamine), a major hormone of pineal gland in vertebrates, is known to be associated with regulation of the dynamic physiological functions in general and has some functions on reproduction in the ovarian follicles in particular. And its antioxidant properties as a scavenger are also reported. The aim of this study was to investigate the effect of melatonin on the in vitro maturation of mouse germinal vesicle (GV)-stage oocytes. Oocyte maturation, apoptosis, and mRNA expression of melatonin receptor were analyzed in the cumulus cell-enclosed oocytes (CEOs) cultured with melatonin for 18 h. The CEOs were obtained from 3 wk-old ICR female mice cultured in media with 0, 0.1 nM, 10 nM, or 1,000 nM melatonin for 18 h. And then the extrusion of the first polar body was assessed to evaluate the maturation rate. The apoptosis and mRNA expression of melatonin receptor (Mtnr1-a and Mtnr1-b) in cumulus cells of each group were measured by TUNEL assay, ELISA, and real time RT-PCR after in vitro maturation(IVM). The addition of melatonin in the IVM medium significantly improved nuclear maturation of the mouse GV oocytes and the highest maturation rate were obtained from the group treated with 1,000 nM melatonin. Apoptosis was not detected in IVM oocytes, but detected in cumulus cells. And cumulus cells treated with 1,000 nM melatonin exhibited significantly lower apoptosis. In the group treated with 1,000 nM melatonin, the expression of melatonin receptor mRNA was decreased in CEOs. In conclusion, melatonin has a potentially important role for regulating oocyte maturation and reduces the apoptosis of cumulus cells in vitro.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.2
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• pp.137-143
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• 2006

Three candidate processes(ozone alone, UV alone and ozone/UV combined processes) were evaluated for the removal of diethyl phthalate(DEP). Of the candidates, the ozone/UV process showed the highest removal efficiency of DEP. To elucidate a major oxidant for DEP oxidation in the ozone/UV process, the effects of pH and hydroxyl radical($OH^{\circ}$) scavenger were investigated. As a result, it was found that $OH^{\circ}$ plays a important role for DEP elimination. Meanwhile, the direct reaction between ozone and DEP was negligible. Observing the pseudo first-order rate of DEP removal in ozone alone and ozone/UV processes, the different pattern was obtained from two processes. The ozone/UV process was well plotted following the pseudo first-order. but in the ozone alone process the rate was divided into fast and slow phases. DEP degradation characteristics in ozone alone and ozone/UV was also investigated by observing the HPLC spectrum. We detected unknown compounds that were guessed to DEP byproducts and observed the formation and disappearance of the unknown compounds according to reaction time. Observing of high removal of TOC in ozone/UV combined process, it was found that DEP and DEP byproducts are completely oxidized by ozone/UV combined process.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.6
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• pp.642-650
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• 2005

This research investigates the effects of adding oxidants such as $Fe^0$, $Fe^{2+}$, and ${S_2O_8}^{2-}$ in the sonolysis of 1,4-D. Results indicate that the degradation could be divided into two steps (initiation and acceleration) kinetically. The initial portion agreed with zero-order expression, while the second portion could be fitted with pseudo first-order expression. In the presence of ${HCO_3}^-$, as a radical scavenger, the degradations of 1,4-D and TOC were suppressed, indicating that OH radical is an important factor in the sonolysis. The overall degradation efficiency of 79.0% in the sonolysis was achieved within 200 minutes. While $Fe^0$, $Fe^{2+}$, and ${S_2O_8}^{2-}$ were individually combined with sonication, the degradation efficiency of 1,4-D increased 18.6%, 19.1%, and 16.5% after 200 min, respectively. The addition of oxidants not only changed the kinetic model from zero to pseudo first order at initiation step, but also increased the rate constants in the acceleration step. The addition of oxidants in the sonolysis of 1,4-D also improved the mineralization of 1,4-D, however, the effect of adding oxidants on the rate increase was similar regardless of the oxidants.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.7
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• pp.649-655
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• 2010

This study investigated the degradation of phenol using sonication and/or UV-C. The effects of frequency, temperature, pH in solution, argon purging, with UV intensity were estimated in sonication-only, UV-only, and the combined reaction of sonication with UV. The optimum condition for degrading phenol in the sonication-only reaction was 35 kHz, $5^{\circ}C$, and pH 4. As this condition approximately 30% degradation of phenol was achieved within 360 min. However, phenol in the UV-only at $19.3\;mw/cm^2$ under the same condition was completely degraded within 60 min. In the combined system of sonication with UV, the degradation of phenol was well fitted to first-order rate model, and phenol was completely degraded within 360 min and 45 min at UV intensity of $7.6\;mW/cm^2$($17.3{\times}10^{-3}\;min^{-1}$) and $19.3\;mW/cm^2$($138.1{\times}10^{-3}\;min^{-1}$), respectively. Adding methanol, as a radical scavenger, in the phenol degradation in the sonication reaction indicates that OH radical is a major factor in the degradation of phenol. The order of degradation efficiencies of phenol was in the order of as follows; combined reaction of sonication with UV > UV-only > sonication-only.

• Journal of the Korean Wood Science and Technology
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• v.34 no.5
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• pp.42-51
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• 2006

The this study, the effect of rice husk flour (RHF) as scavenger on formaldehyde emission rate and formaldehyde content from urea-formaldehyde (UF) resin bonded RHF content wood particleboards (PB). Two type of particle size ($30{\mu}m$ and $300{\mu}m$) of RHF was premixed with the UF resin at 5% and 15% by weight. The performance of UF resins is greatly influenced by the curing characteristics in their curing processing. The curing behavior was monitored activation energy ($E_a$) by DSC and pH variation according to RHF contents. PB with dimensions of $27cm{\times}27cm{\times}0.7cm$ was prepared at a specific gravity of 0.75 using $E_1$ and $E_2$ class UF resins. Formaldehyde emission and formaldehyde content from RHF filled PB bonded with UF resin was measured by 24 h desiccator and perforator method, respectively. RHF causes an increased pH of UF resin. $E_a$ of the modified UF resin decreased independently of RHF particle size. As the pH and the $E_a$ variation of the UF resin containing RHF increased, the amount of formaldehyde content decreased. The formaldehyde emission and formaldehyde content levels of the PB bonded with 15 wt% of $30{\mu}m$ RHF and $E_2$ type UF resin were low and satisfied grade $E_1$, as measured by 24 h desiccator and perforator method. The result of a comparison between 24 h desiccator and perforator test using PB showed that the linear regression analyses show a good correlation between the results for the 24 h desiccator and the perforator tests. The linear regression of a correlation between the desiccator and the perforator was Y=4.842X-0.064 ($R^2=0.989$). RHF was effective at reducing formaldehyde emission and formaldehyde content in urea-formaldehyde adhesives when used as scavenger.

• Journal of Life Science
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• v.25 no.11
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• pp.1235-1243
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• 2015

Hwangheuk-san (HHS) is a Korean multi-herb formula comprising four medicinal herbs. HHS, which was recorded in “Dongeuibogam,” has been used to treat patients with inflammation syndromes and digestive tract cancer for hundreds of years. However, little is known about its anti-tumor efficacy. The present study investigated the pro-apoptotic effect and mode of action of HHS against AGS human gastric carcinoma cells. HHS inhibited the cell growth of AGS cells in a dose-dependent manner, which was associated with the induction of apoptotic cell death, as evidenced by the formation of apoptotic bodies, chromatin condensation, and an accumulation of cells in the sub-G1 phase. HHS-induced apoptotic cell death was associated with the up-regulation of pro-apoptotic Bax protein expression, down-regulation of antiapoptotic Bcl-2 protein, and the release of cytochrome c from mitochondria to the cytosol. The treatment of AGS cells with HHS significantly elevated the generation of reactive oxygen species (ROS). Additionally, apoptosis-inducing concentrations of HHS induced the activation of both caspase-9 and -8, initiator caspases of the mitochondrial-mediated intrinsic and death receptor-mediated extrinsic pathways, respectively, and caspase-3, accompanied by proteolytic degradation of poly (ADP-ribose)-polymerase. However, ROS scavenger and pan-caspases inhibitor significantly blocked HHS-induced growth inhibition and apoptosis. Taken together, these findings suggest that HHS induces apoptosis through ROS- and caspase-dependent mechanisms and that HHS may be a potential chemotherapeutic agent for the control of human gastric cancer.