• Journal of Applied Biological Chemistry
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• 제63권3호
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• pp.197-201
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• 2020

In order to enhance the extraction efficiency of flavonoid from Saururus chinensis, carbohydrate-hydrolyzing enzyme Viscozyme L aided extraction techniques have been studied. Then flavonoid composition, as well as quercetin, were also identified using UV/Vis, HPLC/MS, and ¹H-NMR. The results showed that favorable extraction conditions were Viscozyme L concentration of 0.25 mg/g, pH 4.2, reaction at 45 ℃ for 12 h. Under the favorable extraction condition, total flavonoid yield (37.9 mg/g) and quercetin yield (0.86 mg/g) increased by about 2.0 and 9.6 times, respectively, compared to control group. Interestingly, as a significant flavonoid of S. chinensis, flavonoid glycones rutin was hydrolyzed to aglycones quercetin by Viscozyme L. These findings provide scientific and theoretical support for the development quercetin-rich products, which was quickly absorbed by the human body than rutin.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.319.3-320
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• 2002

Saururus chinensis Baill (Saururaceae) is a perennial plant that has been used in the treatment of edema. jaundice and gonorrhea in Korean folk medicine. Houttuynin sodium bisulphate (HSB), alpha hydroxyl-capryl-ethyl-sodium-sulphonate. is a product formed by reacting sodium bisulphate with houttuynin. which is obtained from a medicinal herb Houttuynia cordata Thunb. (omitted)

• 한국자원식물학회지
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• 제15권1호
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• pp.67-71
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• 2002

삼백초의 3요소 시비 적량을 구명하고자 3요소 시비수준을 무비구 등 3요소별 4수준으로 11조합하여 시험을 수행한 결과를 요약하면 다음과 같다. 1. 토양의 이화학적 변화는 pH, 유기물, 마그네슘, CEC 그리고 EC는 시험 전에 비해 시험 후에 높은 경향을 보였으나, 인산, 칼리 그리고 칼슘은 상반된 경향이었다. 2. 건엽중은 무비구 281kg/10a에 비하여 3요소 시비구에서 26~108kg가 많았으며, 건근중은 무비구 610kg/10a에 비하여 3요소 시비구에서 19~157kg가 많았다. 3. 2년생 삼백초의 3요소 시비적량은 질소 7.1, 인산 2.5, 칼리 4.2kg/10a이었다.

• 한국약용작물학회지
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• 제11권2호
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• pp.148-154
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• 2003

홍삼(panax ginseng C.A. Meyer) 추출물 단독의 항산화 활성과 홍삼추출물에 삼백초(Saururus chinensis(Lour.) Baill.)와 복분자(Rubus coreanus Miq.) 추출물을 농도별로 보충 급여 하였을 때 나타나는 상승효과를 알아보기 위하여 Sprague Dawley rat에 이들 추출물을 8주간 섭취시킨 후 희생시켜 간장 cytosol중의 superoxide dismutase, catalase, glutathione peroxidase, GST 등의 항상화 효소계 활성을 측정하였다. 그 결과, 홍삼급여군의 superoxide dismutase, catalase, glutathione peroxidase의 효소활성이 대조군에 비해 농도 의존적으로 높았다. 특히 홍삼 단독보다는 삼백초와 복분자를 보충급여시 상승효과가 높았으며 급여기간이 길수록 그 효과가 높았다.

• Nutrition Research and Practice
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• 제1권2호
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• pp.100-104
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• 2007

Saururus chinensis Baill was reported to inhibit ${\alpha}-glucosidase$ in vitro and flatten postprandial increase in blood glucose in streptozotocin (STZ)-induced diabetic rats. We studied the effect of chronic consumption of S. chinensis Baill on blood glucose and lipid profile in STZ-induced diabetic male rats fed high fat diet. Male rats weighing 100-120 g were fed 30% fat diet with and without 10% freeze-dried leaves of S. chinensis Baill for 7 weeks after 1 week of adaptation. The rats were rendered diabetic by intravenous injection of STZ (60 mg/kg) after 6-week feeding of the assigned diets. At 1 week after the injection, the rats were sacrificed after an overnight fast. Plasma glucose ($380.2{\pm}14.4mg/dL$), total cholesterol ($93.9{\pm}7.9mg/dL$) and triglyceride levels ($123.6{\pm}7.5mg/dL$) of the S. chinensis Baill group were significantly lower than those of the control group ($418.1{\pm}12.0mg/dL,\;119.9{\pm}9.4mg/dL,\;152.0{\pm}10.3mg/dL$, respectively, p<0.05). Chronic consumption of S. chinesis Baill significantly decreased maltase activity of the small intestinal mucosa ($120.1{\pm}8.7U/g$) protein compared with the control group ($96.8{\pm}7.0U/g protein, p<0.05). These results suggest that S. chinensis Baill have hypoglycemic and hypolipidemic effects by inhibiting ${\alpha}-glucosidase$ activity in the animal model of diabetes mellitus.

• 동의생리병리학회지
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• 제19권1호
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• pp.146-151
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• 2005

We investigated the effect of Saururus chinensis (Lour.) Baill (SCB) on allergy in mice. We conformed compound 48/80-induced mesenteric mast cell degranulation, active systemic anaphylatic shock and histamine release. Also observed acetic acid-induced vascular permeability and anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. SCB inhibited mesenteric mast cell degranulation and active systemic anaphylatic shock induced by compound 48/80 dose-dependently. When SCB was pretreated by intra-peritoneal injection, the plasma histamine levels were reduced. SCB also significantly inhibited acetic acid-induced vascular permeability and anti-DNP IgE-mediated passive cutaneous anaphylaxis. In addition, SCB reduced IL-10 mRNA expression of the lung on ovalbumin-induced allergy. These results indicate that SCB inhibits allergy.

• Korean Journal of Acupuncture
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• 제21권3호
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• pp.97-104
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• 2004

Objective We investigated the effect of Saururus chinensis (Lour.) Baill. aquacupuncture (SCB) on anaphylaxis in mice. Methods : We conformed compound 48/80-induced mesenteric mast cell degranulation, active systemic anaphylatic shock and histamine release. Also observed acetic acid-induced vascular permeability and anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. Results : SCB inhibited mesenteric mast cell degranulation and active systemic anaphylatic shock induced by compound 48/80 dose-dependently. When SCB was pretreated by intra-peritoneal injection, the serum histamine levels were reduced. SCB also significantly inhibited acetic acid-induced vascular permeability. In addition, SCB showed a significant inhibitory effect on anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. Conclusion : These results indicated that SCB inhibits anaphylatic reaction.

• 한국식품위생안전성학회지
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• 제18권3호
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• pp.166-170
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• 2003

삼백초가 TCDD 투여로 유발된 rat의 지질대사에 미치는 효과를 알아보기 위해 NTT, TTA, STT군으로 각각 10마리씩 나누어 TCDD를 투여하고 4주 동안 물질을 투여하였다. 4주 후 희생시켜서 혈청과 간의 지질대사를 관찰한 결과는 다음과 같다. 1. Total Cholesterol에서 삼백초를 투여한 STT군은 NTT군 비교해서 14.46% 증가하였으며, 혈청 중의 HDL-Cholesterol은 SST군이 TTA군과 비교하여 21.29% 증가하였고 94.98%로 엑제되었다. LDL-Cholesterol에서는 STT군이 TTA군 보다 12.86% 감소하였으며, 48.34%로 억제되었다. 2. 혈청중의 TG의 함량은 TTA군이 NTT군에 비해 34.84% 증가하였다. TTA 군에 비해 STT군은 7.06% 감소하였으며, 18.9% 억제시킨다 3. 간조직 중의 MDA량은 STT군이 TTA군에 비해 17.14% 감소하였고, 32.72%의 억제율을 보였다.

• 대한예방한의학회지
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• 제8권2호
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• pp.81-98
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• 2004

1. The cell viability was determined by MTT method. Their cytotoxic activities against three cancer cell lines such as A549, MDA-MB-231 and SNU-C4 cell line were tested. Among them, The methanol extract of Saururus chinensis Baill. showed the strongest cytotoxic effect against SNU-C4 cells. These results suggest that the methanol extract of Saururus Chinensis Baill. possessed a potential antitumorous agent. 2. In vitro the antitoxic activity of ethanol extract of Saururus Chinensis Bail on NIH 3T3 fibroblasts was evaluate by the MTT (3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyl-2H-tetra캐lium bromide) and SRB (sulforhodamine B protein) assays. The number of NIH 3T3 fibroblasts were increased and tend to regenerate. These results suggest that Saururus Chinensis Bail extract retains a potential antitoxic activity. 3. Complexation of Cd (II) ion with ligands such as quercetin has been determined by UV-vis spectrophotometric method in tris buffer solution at various pH. It was found that only 1 : 1 Cd-complex is formed by the interaction between catechol moiety in ring B and cadmium [Cd(II)] in aqueous solution. The spectral parameters for Cd-complex were determined by Beer's law at various pH. It has shown that 1 : 1 Cd-complex has a maximum absorbance and red shift by the alkaline pH.

• 한국약용작물학회지
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• 제24권6호
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• pp.458-463
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• 2016

Background: The objective of the present study was to investigate the effect of storage conditions on the growth of Saururus chinensis (Lour.) Baill. rootstock. Methods and Results: Rootstocks of S. chinensis were stored in either soil or vermiculite that had been treated with a control treatment, diluted wood vinegar (50 or 100-fold), DF-100 (50-fold), or 1-naphthylacetamide and at $5^{\circ}C$ or $15^{\circ}C$. After 8 weeks, the stored roots were planted in the field, and both plant height and leaf number were observed after transplantation. The greatest number of leaves ($5.60{\pm}0.80$) was produced by roots that had been stored in soil treated with 100 fold dilution of wood vinegar and at $5^{\circ}C$. Meanwhile the maximum plant height ($6.92{\pm}0.78cm$) at 30 d after transplanting was observed for rootstocks that had been stored in soil treated with the 100 fold dilution of wood vinegar and at $15^{\circ}C$, whereas the maximum plant height at 60 d after transplanting ($26.46{\pm}0.71cm$) was observed for rootstocks that had been stored in soil treated with the 100-fold dilution of wood vinegar and at $5^{\circ}C$. Therefore, the storage of rootstocks in soil treated with the 100-fold dilution of wood vinegar and at temperatures at or below $5^{\circ}C$ was most effective, and it can be used to prevent the decay of roots during the postharvest management of S. chinensis rootstocks. Conclusions: The results of the present study indicate that, among the parameters examined, the storage of roots in soil that had been treated with the 100-foil dilution of wood vinegar is the most effective method for improving the growth of S. chinensis.