• Bulletin of the Korean Chemical Society
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• 제31권5호
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• pp.1215-1218
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• 2010

We demonstrate the possible application of the sandwich type surface-enhanced Raman scattering (SERS) immunoassay using antigen-antibody binding for detection of prostate-specific antigen (PSA) in cancer cells. In this sandwich type of SERS immunoassay, to capture antigens onto the immobilized layer of antibodies on the gold substrate we prepared the monolayer of gold nanoparticles on the APTMS-derivatized surface of a glass slide by using the SAM technique. This sandwich type of SERS immunoassay in which antigens on the substrate specifically capture antibodies on a Raman reporter (DSNB coated gold nanoparticles with R6G) could successfully detect PSA at low levels. A strong SERS spectrum of Raman reporter was observed only with a substrate in which PSA is present.

• 전기화학회지
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• 제7권1호
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• pp.44-48
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• 2004

The two-component type enzyme amplified amperometric DNA assay is described to use an ambient $O_2$ of the substrate of the DNA labeling enzyme. Although the assay detects DNA only at > 0.5M concentration, a concentration $\~10^6$ fold higher than the sandwich-type enzyme amplified amperometric DNA assay, it can be run with an always available substrate. The assay utilizes screen-printed carbon electrodes (SPEs) which were pre-coated by a co-electrodeposited film of an electron conducting redox hydrogel and a 37-base long single-stranded DNA sequence. The DNA in the electron conducting film hybridizes and captures, when present, the 37-base long detection-DNA, which is labeled with bilirubin oxidase (BOD), an enzyme catalyzing the four-electron reduction of $O_2$ to water. Because the redox hydrogel electrically connects the BOD reaction centers to the electrode, completion of the sandwich converts the film from non-electrocatalytic to electrocatalytic for the reduction of $O_2$ to water when the electrode is poised at 200 mV vs. Ag/hgCl. The advantage or the assay over the earlier reported sandwich type enzyme amplified amperometric DNA assay, in which the amplifying enzyme was horseradish peroxidase, is that it utilizes ambient $O_2$ instead of the less stable and naturally unavailable $H_2O_2$.

• Steel and Composite Structures
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• 제25권2호
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• pp.157-175
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• 2017

The novelty of this work is the use of a new displacement field that includes undetermined integral terms for analyzing thermal buckling response of functionally graded (FG) sandwich plates. The proposed kinematic uses only four variables, which is even less than the first shear deformation theory (FSDT) and the conventional higher shear deformation theories (HSDTs). The theory considers a trigonometric variation of transverse shear stress and verifies the traction free boundary conditions without employing the shear correction factors. Material properties of the sandwich plate faces are considered to be graded in the thickness direction according to a simple power-law variation in terms of the volume fractions of the constituents. The core layer is still homogeneous and made of an isotropic material. The thermal loads are assumed as uniform, linear and non-linear temperature rises within the thickness direction. An energy based variational principle is employed to derive the governing equations as an eigenvalue problem. The validation of the present work is checked by comparing the obtained results the available ones in the literature. The influences of aspect and thickness ratios, material index, loading type, and sandwich plate type on the critical buckling are all discussed.

• Structural Engineering and Mechanics
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• 제58권3호
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• pp.397-422
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• 2016

In the present work, a simple first-order shear deformation theory is developed and validated for a variety of numerical examples of the thermal buckling response of functionally graded sandwich plates with various boundary conditions. Contrary to the conventional first-order shear deformation theory, the present first-order shear deformation theory involves only four unknowns and has strong similarities with the classical plate theory in many aspects such as governing equations of motion, and stress resultant expressions. Material properties and thermal expansion coefficient of the sandwich plate faces are assumed to be graded in the thickness direction according to a simple power-law distribution in terms of the volume fractions of the constituents. The core layer is still homogeneous and made of an isotropic material. The thermal loads are considered as uniform, linear and non-linear temperature rises within the thickness direction. The results reveal that the volume fraction index, loading type and functionally graded layers thickness have significant influence on the thermal buckling of functionally graded sandwich plates. Moreover, numerical results prove that the present simple first-order shear deformation theory can achieve the same accuracy of the existing conventional first-order shear deformation theory which has more number of unknowns.

• Steel and Composite Structures
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• 제27권3호
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• pp.273-283
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• 2018

Nonlinear low velocity impact response of sandwich beam with laminated composite face sheets and soft core is studied based on Extended High Order Sandwich Panel Theory (EHSAPT). The face sheets follow the Third order shear deformation beam theory (TSDT) that has hitherto not reported in conventional EHSAPT. Besides, the two dimensional elasticity is used for the core. The nonlinear Von Karman type relations for strains of face sheets and the core are adopted. Contact force between the impactor and the beam is obtained using the modified Hertz law. The field equations are derived via the Ritz based applied to the total energy of the system. The solution is obtained in the time domain by implementing the well-known Runge-Kutta method. The effects of boundary conditions, core-to-face sheet thickness ratio, initial velocity of the impactor, the impactor mass and position of the impactor are studied in detail. It is found that each of these parameters have significant effect on the impact characteristics which should be considered. Finally, some low velocity impact tests have been carried out by Drop Hammer Testing Machine. The contact force histories predicted by EHSAPT are in good agreement with that obtained by experimental results.

• Structural Engineering and Mechanics
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• 제67권3호
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• pp.219-232
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• 2018

This paper deals with the static bending of various types of FGM sandwich plates resting on two-parameter elastic foundations in hygrothermal environment. The elastic foundation is modeled as Pasternak's type, which can be either isotropic or orthotropic and as a special case, it converges to Winkler's foundation if the shear layer is neglected. The present FGM sandwich plate is assumed to be made of a fully ceramic core layer sandwiched by metal/ceramic FGM coats. The governing equations are derived from principle of virtual displacements based on a shear and normal deformations plate theory. The present theory takes into account both shear and normal strains effects, thus it predicts results more accurate than the shear deformation plate theories. The results obtained by the shear and normal deformation theory are compared with those available in the literature and also with those obtained by other shear deformation theories. It is concluded that the present results are slightly deviated from other results because the normal deformation effect is taken into account. Numerical results are presented to show the effects of the different parameters, such as side-to-thickness ratio, foundation parameters, aspect ratio, temperature, moisture, power law index and core thickness on the stresses and displacements of the FG sandwich plates.

• Structural Engineering and Mechanics
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• 제63권5호
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• pp.585-595
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• 2017

This study aimed to presents a simple analytical approach to investigate the thermal buckling behavior of thick functionally graded sandwich by employing both the sinusoidal shear deformation theory and stress function. The material properties of the sandwich plate faces are continuously varied within the plate thickness according to a simple power-law distribution in terms of the volume fractions of the constituents. The core layer is still homogeneous and made of an isotropic material. The thermal loads are considered as uniform, linear and non-linear temperature rises across the thickness direction. Numerical examples are presented to prove the effect of power law index, loading type and functionally graded layers thickness on the thermal buckling response of thick functionally graded sandwich.

• 한국산업융합학회 논문집
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• 제20권2호
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• pp.133-140
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• 2017

This paper is a study of the central structural unit model of the sandwich core structure. The applied model is based on the honeycomb structure formed by the truss, the H-shaped honeycomb structure formed by adding the truss of H shape to the space of the center portion, and the honeycomb structure formed by the plate. Applied material property is AISI 304 stainless steel, which has cost effectiveness and easy to get near place. The truss diameter of the model is three different type: 1mm, 2mm and 3mm. ABAQUS software is obtained to do the analysis and applied test is quasi-static loading. Boundary conditions for the analysis are that vertical direction loading at top place without any rotation and bottom surface is fixed. The test results show that the H-truss model has the highest stiffness and yield strength. Therefore, it is hoped that more and more researching for the development of a unit model in sandwich core structure has been investigating and that the developed sandwich core model can be applied into various industrial fields such as mechanical or aerospace industries.

• Steel and Composite Structures
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• 제33권1호
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• pp.93-109
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• 2019

In the present study, vibration analysis of double bonded micro sandwich cylindrical shells with saturated porous core and carbon/boron nitride nanotubes (CNT/BNNT) reinforced composite face sheets under multi-physical loadings based on Cooper-Naghdi theory is investigated. The material properties of the micro structure are assumed to be temperature dependent, and each of the micro-tubes is placed on the Pasternak elastic foundations, and mechanical, moisture, thermal, electrical, and magnetic forces are effective on the structural behavior. The distributions of porous materials in three distributions such as non-linear non-symmetric, nonlinear-symmetric, and uniform are considered. The relationship including electro-magneto-hydro-thermo-mechanical loadings based on modified couple stress theory is obtained and moreover the governing equations of motion using the energy method and the Hamilton's principle are derived. Also, Navier's type solution is also used to solve the governing equations of motion. The effects of various parameters such as material length scale parameter, temperature change, various distributions of nanotube, volume fraction of nanotubes, porosity and Skempton coefficients, and geometric parameters on the natural frequency of double bonded micro sandwich cylindrical shells are investigated. Increasing the porosity and the Skempton coefficients of the core in micro sandwich cylindrical shell lead to increase the natural frequency of the structure. Cylindrical shells and porous materials in the industry of filters and separators, heat exchangers and coolers are widely used and are generally accepted today.

• Steel and Composite Structures
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• 제32권6호
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• pp.753-767
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• 2019

Vibration control in mechanical equipments is an important problem where unwanted vibrations are vanish or at least diminished. In this paper, free vibration active control of the porous sandwich piezoelectric polymeric nanocomposite microbeam with microsensor and microactuater layers are investigated. The aim of this research is to reduce amplitude of vibration in micro beam based on linear quadratic regulator (LQR). Modified couple stress theory (MCST) according to sinusoidal shear deformation theory is presented. The porous sandwich microbeam is rested on elastic foundation. The core and face sheet are made of porous and three-phase carbon nanotubes/resin/fiber nanocomposite materials. The equations of motion are extracted by Hamilton's principle and then Navier's type solution are employed for solving them. The governing equations of motion are written in space state form and linear quadratic regulator (LQR) is used for active control approach. The various parameters are conducted to investigate on the frequency response function (FRF) of the sandwich microbeam for vibration active control. The results indicate that the higher length scale to the thickness, the face sheet thickness to total thickness and the considering microsensor and microactutor significantly affect LQR and uncontrolled FRF. Also, the porosity coefficient increasing, Skempton coefficient and Winkler spring constant shift the frequency response to higher frequencies. The obtained results can be useful for micro-electro-mechanical (MEMS) and nano-electro-mechanical (NEMS) systems.