• Title/Summary/Keyword: Sampling Period

검색결과 1,201건 처리시간 0.026초

Improvement of Antigen Blotting in a Tissue Blot Immunobinding Assay for the Detection of Two Chili Pepper Viruses

  • Han, Jung-Heon;Shin, Jun-Sung;Kim, Young-Ho;Kim, Byung-Dong
    • Journal of Microbiology and Biotechnology
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    • 제17권11호
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    • pp.1885-1889
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    • 2007
  • The tissue blot immunobinding assay (TBIA) is widely used for the detection and localization of plant viruses in various plant tissues. The basic experimental procedures of TBIA sampling and blotting were simplified using commercially available micropipette tips. This method was termed the ring-blot immunobinding assay (R-BIA), as the blot on the membrane forms a ring shape. The detection efficacy of R-BIA was tested for two chili pepper viruses, pepper mild mottle tobamovirus (PMMoV) and pepper mottle potyvirus (PepMoV), following the optimized serological procedures of TBIA (length of the incubation period and BSA concentration, and primary and secondary antibodies). Sensitivity of the R-BIA was about 1 ng/ml of purified PMMoV in pepper leaf sap from a healthy pepper plant. R-BIA also showed high specificity in the detection of PMMoV and PepMoV. Moreover, the modified sampling and blotting procedures were simpler and more reliable than other TBIA methods (such as whole-leaf blotting and crushed-leaf blotting), suggesting that the R-BIA may be used for medium- to large-scale detection of plant viruses in laboratories with minimal facilities.

가변 윈도우 기법을 적용한 통계적 공정 제어와 퍼지추론 기법을 이용한 소프트웨어 성능 변화의 빅 데이터 분석 (Big Data Analysis of Software Performance Trend using SPC with Flexible Moving Window and Fuzzy Theory)

  • 이동헌;박종진
    • 제어로봇시스템학회논문지
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    • 제18권11호
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    • pp.997-1004
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    • 2012
  • In enterprise software projects, performance issues have become more critical during recent decades. While developing software products, many performance tests are executed in the earlier development phase against the newly added code pieces to detect possible performance regressions. In our previous research, we introduced the framework to enable automated performance anomaly detection and reduce the analysis overhead for identifying the root causes, and showed Statistical Process Control (SPC) can be successfully applied to anomaly detection. In this paper, we explain the special performance trend in which the existing anomaly detection system can hardly detect the noticeable performance change especially when a performance regression is introduced and recovered again a while later. Within the fixed number of sampling period, the fluctuation gets aggravated and the lower and upper control limit get relaxed so that sometimes the existing system hardly detect the noticeable performance change. To resolve the issue, we apply dynamically tuned sampling window size based on the performance trend, and Fuzzy theory to find an appropriate size of the moving window.

문치가자미 RNA/DNA의 일주기적 변화 (Circadian Variation of the RNA/DNA Ratios in Marbled Flounder Pleuronectes yokohamae)

  • 곽우석
    • 한국어류학회지
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    • 제22권4호
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    • pp.267-272
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    • 2010
  • 문치가자미 Pleuronectes yokohamae의 RNA/DNA비 일주기 변화를 조사하기 위하여 부화후 89일, 전장 $23.2{\pm}0.2mm$의 치어를 48시간 동안 일정한 시간 간격으로 채집하였다. RNA/DNA비는 주간(0800, 1100, 1400, 1700)에 높았고 야간(2000, 2300, 0200, 0500 hours)에는 낮게 나타났다. 이번 연구결과에서 문치가자미의 생화학적 일주기 변화를 확인하였고, 이와 같은 RNA/DNA비의 일주기적 변화를 고려하여 시료 채집시간 결정을 포함한 시료채집 계획작성과 data분석이 필요할 것으로 생각된다.

오수에 따른 식물성 Plankton의 분포조사 (A Study on the phytoplankton distribution in polluted water.)

  • 김병구
    • 한국환경보건학회지
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    • 제5권1호
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    • pp.61-65
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    • 1978
  • To investigate the phytoplankton distribution effected by polluted water, this research was carried out at eight sampling places in Tae-jun and one sampling place in Kang-kyong during the period from May 10 to July 25, 1977. The results are as follows. 1) The range of water temperature was 21.0~36.0$\circ$C (May 10~July 25), and pH value was in the range of 3.5~11.0. 2) BOD was generally high (140~432mg/l) 3) At the places where BOD was high, dominant species were Aphanocapsa rivularis, Microcystis aeruginosa and Oscillatoria sp. belonging to Cyanophyta, Chlorella sp. belonging to Chlorophyta, Euglena sp. belonging to Euglenophyta, and Navicula exqua and Navicula sp. belonging to Chrysophyta. 4) At the places where the range of pH value was 3.5~3.8 and the range of BOD value was 134~148mg/l, observable species were Scenedesmus ellipsoideus and Pandorina morurn belonging to Chlorophyta, Navicula exqua belonging to Chrysophyta and Euglena sp. belonging to Euglenophyta. 5) At the places where the range of pH value was 9.0~11.0 and the range of BOD value was 214~220mg/l, a few observable species were Scenedesmus carinatus, Scenedesmus ellipsoideus, Scenedesmus bijuga and Scenedesmus obundance belonging to Chlorophyta.

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심자도 신호획득을 위한 실시간 256-채널 12-bit 1ks/s 하드웨어 (Real-time 256-channel 12-bit 1ks/s Hardware for MCG Signal Acquisition)

  • 유재택
    • 대한전기학회논문지:시스템및제어부문D
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    • 제54권11호
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    • pp.643-649
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    • 2005
  • A heart diagnosis system adopts Superconducting Quantum Interface Device(SQUD) sensors for precise MCG(MagnetoCardioGram) signal acquisitions. Such system needs to deal with hundreds of sensors, requiring fast signal sampling md precise analog-to-digital conversions(ADC). Our development of hardware board, processing 64-channel 12-bit in 1 ks/s speed, is built by using 8-channel ADC chips, 8-bit microprocessors, SPI interfaces, and specially designed parallel data transfers between microprocessors to meet the 1ks/s, i.e. 1 mili-second sampling interval. We extend the design into 256-channel hardware and analyze the speed .using the measured data from the 64-channel hardware. Since our design exploits full parallel processing, Assembly level coding, and NOP(No Operation) instruction for timing control, the design provides expandability and lowest system timing margin. Our result concludes that the data collection with 256-channel analog input signals can be done in 201.5us time-interval which is much shorter than the required 1 mili-second period.

Does Audit Committee Quality Mediate Determinants of Intellectual Capital Disclosure?

  • ASTUTI, Resa Nur;FACHRURROZIE, Fachrurrozie;AMAL, Muhammad Ihlashul;ZAHRA, Siti Fatimah
    • The Journal of Asian Finance, Economics and Business
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    • 제7권7호
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    • pp.199-208
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    • 2020
  • This study investigates the direct and indirect effects, mediated by audit committee quality, of managerial ownership, institutional ownership, and profitability on intellectual capital (IC) disclosure. The object observed of this study is companies listed on the Indonesia Stock Exchange (IDX) in the 2014-2018 period that are classified as high intellectual capital-intensive industries. Based on the sampling method, purposive sampling, 51 companies were selected as samples. This study used path analysis techniques with IBM SPSS version 25 to study the direct and indirect influences of managerial ownership, institutional ownership, and profitability toward IC disclosure. The results of this study show that managerial ownership, profitability and audit committee quality have a significant positive effect on IC disclosure whereas institutional ownership has significant negative effect on IC disclosure. This study also provides empirical evidence, supported by the sobel test, that the audit committee quality is able to mediate the effect of institutional ownership and profitability on IC disclosure. However, the audit committee quality is not able to mediate the effect of managerial ownership on IC disclosure. These findings develop and strengthen the results of prior studies related to the implementation of signaling theory and agency theory in devoting more understanding about IC disclosure.

생활시간 연구를 위한 측정도구의 비교 : 경험표집법과 시간일지 (A Systematic Comparison of Time Use Instruments: Time Diary and Experience Sampling Method)

  • 정재기
    • 한국조사연구학회지:조사연구
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    • 제9권1호
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    • pp.43-68
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    • 2008
  • 이 연구는 생활시간 연구를 위한 두 도구를 비교한다. 시간일지와 경험표집법, 이전의 연구들이 이 두 도구가 집합적 측정치에서 유사하다는 것을 보여 왔지만, 각 도구의 개별기록의 일치율을 비교한 연구는 없었다. 이 두 도구에 모두 응답한, 시카고 대학의 알프레드 슬로안 센터에서 행해진 500가족 스터디의 일부 표본을 통하여, 이 연구는 두 도구를 비교하고 상대적 장단점을 평가하였다. 연구결과는 시간일지가 보다 덜 편향된 행동시간 측정치를 제공하는 데 반해, 경험표집법이 일상생활에 대해 보다 구체화된 서술을 가능하게 함을 시사하고 있다. 후속연구를 위한 함의가 논의되었다.

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형광물질을 이용한 대기 중 오존 Passive Sampler의 개발 (Development of a Passive Sampler using a Fluorescence Material for the Ambient Ozone)

  • 임봉빈;정의석;김선태
    • 한국대기환경학회지
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    • 제20권4호
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    • pp.483-491
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    • 2004
  • The purpose of this study is to develop an ozone passive sampler and to evaluate its performance p-Acetamidophenol using as the reagent for ozone reacts specifically with the ambient ozone to produce a fluorescence material (p-acetamidopheonl dimer). The volume of absorbent solution and the extraction time determined at suitable conditions for measuring ozone were 100$\mu$L and 60 min, respectively. The changes of fluorescence were observed with incresing the storage period of passive samplers in ambient air. but the cool storage in a refrigerator did not remarkably influence the increase of fluorescence. The measurement for the precision oi the passive sampling was carried out with duplicate measurement of passive samplers. The intra-class correlation coefficients of passive samplers using dry and wet filters were 0.992 and 0.962, respectively The results from field validation tests indicated practical agreement (dry filter: r=0.963, wet filter: r=0.995) between the passive sampler and an UV photometric $O_3$ analyzer. The limit of quantification of ozone passive samplers with sampling time of 8 hr (wet filter) and 24 hr (dry filter) were 8.0 ppb and 2.7 ppb, respectively.

Development of a Novel Sampling Technique for Natureal VOC Emissions

  • Kim, Jo-Chun
    • Journal of Korean Society for Atmospheric Environment
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    • 제17권E2호
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    • pp.61-70
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    • 2001
  • In recent years there have been growing interests in the potential environmental effects of global climate change. Of specific interests is the role that climate change may play in altering natural volatile organic compound.(NVOC) emissions from trees and the subsequent impact of this perturbation on air quality and ozone formation. A novel vegetation enclosure chamber method was designed and constructed of Tedlar in order to estimate more accurate and precise NVOC emission rates of either small whole plants or the branches of large trees. The enclosure chamber was initially tested in the laboratory and also successfully evaluated in the field. Overall precision for this enclosure was estimated as RSD<10%(n=9). The overall errors associated with the enclosure method in a laboratory system might be relatively small (say<$\pm$15%); however, they might be rather large(say$\pm$40%) in a field-based system. Two consecutive samples were collected on each sampling day from the two pine species during the test period. Slash pine studies showed that the absolute percentage difference between the first and second samples varied from 0.33 to 29%. The percent differences between consecutive emission for loblolly pines varied from 0.74 to 24.2%.

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Corporate Governance and Value Relevance in Indonesia Manufacturing Companies

  • MURDAYANTI, Yunika;ULUPUI, I Gusti Ketut Agung;PAHALA, Indra;INDRIANI, Susi;SUHERMAN, S.
    • The Journal of Asian Finance, Economics and Business
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    • 제7권11호
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    • pp.335-346
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    • 2020
  • This study aims to describe the role of corporate governance as a moderator in electronic financial disclosure that adopts Extensible Business Reporting Language (XBRL) and Company Characteristics on value relevance. The population in this study was all manufacturing companies listed on the Indonesia Stock Exchange from 2017 to 2018, totaling 166 companies. The sampling technique used purposive sampling method, namely, manufacturing companies that publish fully audited financial statements by December 31 of the year 2017-2018. The method used in this research is a quantitative description using the financial statements of manufacturing companies listed on the Indonesia Stock Exchange that have adopted XBRL during the 2017-2018 period. The data analysis method used is multiple regression analysis with moderating variables. The results of this study show a negative and insignificant effect of XBRL on value relevance, a significant negative effect of size on value relevance, a positive and insignificant effect of growth on value relevance, and a significant positive effect of profit on value relevance; meanwhile, corporate governance moderation variable has an insignificant effect in all hypotheses. Suggestions are to increase the number of variables that have an important role in value relevance and expand the number of research objects to be compared.