• Title/Summary/Keyword: Sample-loading

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A target-specific bioassay for screening of bioactive AHL-analogues from natural products

  • Kim, Young-Hee;Kim, Jung-Sun;Park, Sung-Hoon
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.411-414
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    • 2002
  • Acylated homoserine lactones (AHLs) are membrane-permeant signal molecules responsible for biofilm formation of gram-negative bacteria via a unique mechanism known as quorum sensing. A target specific bioassay employing the AHL-responsive Agrobacterium tumefaciens reporter strain has been developed to identify new AHL-like compounds from natural products, which could be developed into antifouling compounds. By varying the X-gal concentration, incubation time, solvent for sample preparation and the sample loading procedure, it was possible to detect low level AHLs up to $10^1nM$. The length of the acyl chain of the AHLs was found to affect the sensitivity of this bioassay.

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A Basic Study on Micro-Electric Potential accompanied with Specimen Failure during Uniaxial Compressive Test (일축 압축에 의한 시료 파괴 시 수반되는 미소 전위에 대한 기초 연구)

  • Kim, Jong-Wook;Park, Sam-Gyu;Song, Young-Soo;Sung, Nak-Hun;Kim, Jung-Ho;Cho, Seong-Jun
    • Geophysics and Geophysical Exploration
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    • v.10 no.3
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    • pp.203-210
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    • 2007
  • As a part of basic studies on monitoring of landslides and slope stability using SP measurements, micro-electric potentials of rock samples were measured accompanied with the rock failure by a uniaxial loading test were measured. The measurement system consists of a 8 channel A/D converter with 24 bit resolution, uniaxial loading tester, strain gages and 4 sets of electrode attached to a rock sample. Rock samples of granite, limestone, and sandstone were tested. Also, mortar samples were tested in order to monitor electric-potentials of a uniform sample. Micro-electric potentials were detected in all saturated samples and the strength of them increased as the loading force increased. Sandstone samples showed the largest strength of micro-electric potential and it followed limestone and granite samples, which indicates a positive relationship with porosity of rocks. The mechanism generating these micro-electric potential can be explained in terms of electro-kinetics. In case of dry samples, micro-electric potential could be observed only in sandstone samples, where piezoelectric effect played main role due to high contents of quartz in sandstone samples. We found that biggest micro-electric potentials were observed at the electrodes near the crack surface of rock samples. This is very encouraging result that SP monitoring can be applied to predicting landsliding or to estimate collapsing position combining with monitoring of acoustic emissions.

Representation of Hull Form by Aitken's Iterative Interpolation Methods (逐次揷間法에 依한 船型의 數値表現法에 關하여)

  • Kim, Hyo-Chul;Yang, Yeong-Sun
    • Bulletin of the Society of Naval Architects of Korea
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    • v.14 no.3
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    • pp.1-4
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    • 1977
  • The computer aided ship design and construction has become very popular one in a ship yard recently. For one of such a purpose a program is developed with Aitken's iterative interpolation method. From the sample calculation we can conclude that the program has a reliable acquracy for the calculation of hydrostatic data or loading manual. And also the program can be applicable to a ship construction by careful selecting of input data.

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A Study on the Analysis and Control of Voltage Stability (전압안정성 분석 및 제어에 관한 연구)

  • 장수형;김규호;유석구
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.43 no.6
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    • pp.869-876
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    • 1994
  • This paper presents an efficient method to calculate voltage collapse point and to avoid voltage instability. To evaluate voltage stability in power systems, it is necessary to get critical loading points. For this purpose, this paper uses linear programming to calculate efficiently voltage collapse point. Also, if index value becomes larger than given threshold value, voltage stability is improved by compensation of reactive power at selected bus. This algorithm is verified by simulation on the IEEE 14-bus sample system.

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A STUDY OF THE FLAMMABILITY LIMIT OF THE BACKWARD FACING STEP FLOW COMBUSTION

  • Tae-Ho Lee
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 1993.10a
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    • pp.55-61
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    • 1993
  • An experimental investigation was conducted in order to determine the flammability limit of the solid fuel ramjet using the backward facing step flow combustion of the plexiglass grain. In order to get the different step height ratio, the grain was drilled straight forward or stepwise. The Phoenics computer code was adopted in order to compare. the flow patterns of the some sample tests using a non-reacting cold turbulent flow model. The stepwise grain give some loading advantage; specially thin and long shape grain design.

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Back Ananlysis of Soft Ground Behavior Using Measured Results for Test Loading (시험성토 계측결과를 이용한 연약지반 거동의 역해석)

  • 김태훈;정창규;황근배;최용규
    • Proceedings of the Korean Geotechical Society Conference
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    • 2002.03a
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    • pp.469-476
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    • 2002
  • There are many methods to accelerate for consolidation of the soft ground but, in this study, only preloading method was used to improve the soft ground. To measure the settlement of soft ground, surface-settlement plates were installed at several points. To examine settlement behavior of soft ground, back analysis was done using the measured results. In the back analysis, consolidation parameter( $c_{v}$) were obtained and it was compared with test result for undisturbed sample.e.

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Ferroelastic Domain and Refractive Property of $Gd_{2}(MoO_{4})_{3}$ Single Crystal ($Gd_{2}(MoO_{4})_{3}$ 단결정의 강탄성구역과 굴절률특성)

  • Son, Jong-Yoon;Lee, Chan-Ku;Lee, Su-Dae;Kim, Jae-Hyung
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.05b
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    • pp.98-102
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    • 2002
  • We investigated domains and conoscope under the polarizing microscope and the index of refraction on the c-plate GMO which has the ferroelectric and ferroelastic phase at room temperature. To observed the change of refractive index in connection with domain, we developed an apparatus to obtain the refractive index by measuring the Brewster's angle. The resolution of the minimum rotation angle of this apparatus is $0.001^{\circ}$. To obtain the refractive index map on the sample, the moving distance of XY stage loaded sample holder is 60 mm and the minimum moving distance is 0.002 mm. Also, To obtain the indicatrix for single crystal, vertical turntable with sample holder and XY stage was loading on horizontal turntable. The minimum resolution angle of this vertical turntable is $0.001^{\circ}$. We measured the refractive index of transparent materials such as ferroelectrics. In the case of $Gd_{2}(MoO_{4})_{3}$, the Brewster angle is $62.11^{\circ}$ and then, the refractive index is 1.8895 by using He-Ne Laser. Also the refractive distribution of c-plate GMO was obtained with $400{\mu}m{\times}120{\mu}m$.

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Structural and Fatigue Analysis on Shock Absorber Mount of Automobile (자동차의 쇽업쇼바 마운트에 대한 구조 및 피로해석)

  • Han, Moon-Sik;Cho, Jae-Ung
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.11 no.1
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    • pp.125-133
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    • 2012
  • This study aims at structural analysis with fatigue on the shock absorber mount of automobile. Two kinds of mount as original model 1 and reinforced model 2 are applied. Among the cases of nonuniform fatigue loads at both models, 'SAE bracket history' with the severest change of load becomes most unstable but 'Sample history' becomes most stable. In case of 'SAE bracket history' or 'SAE transmission', the maximum fatigue life at model 2 is 5 to 6 times as much as model 1 and the minimum damage at model 2 is decreased 5 to 6 times as much as model 1. In case of 'Sample history' as slow fatigue loading history, the minimum damage at model 2 becomes same as model 1 but the maximum fatigue life at model 2 is decreased more than 17 times as much as model 1. In case of 'Sample History' with the average stress of -$10^4MPa$ to $10^4MPa$ and the amplitude stress of 0MPa to $10^4MPa$, the possibility of maximum damage becomes 3%. This stress state can be shown with 5 times more than the damage possibility of 'SAE bracket history' or 'SAE transmission'. Safe and durable design of shock absorber can be effectively improved by using this study result on mount frame.

Disposable Electrochemical Immunosensors for the Detection of Herbicide (제초제 검출을 위한 전기화학적 일회용 면역센서)

  • Chang, Seung-Cheol
    • Journal of Sensor Science and Technology
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    • v.20 no.1
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    • pp.35-39
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    • 2011
  • A disposable electrochemical immunosensor system has been developed for the detection of herbicide in aqueous samples. Disposable screen printed carbon electrodes(SPCE) were used as basic electrodes and an enzyme, horseradish peroxidase (HRP), and anti-herbicide antibodies was immobilised on to the working electrode of SPCE by using avidin-biotin coupling reactions. An herbicide-glucose oxidase conjugates have been used for the competitive immunoreaction with sample herbicides. The enzymatic reaction between the conjugated glucose oxidase and glucose added generates hydrogen peroxide, which was reduced by the peroxidase immobilised. The latter process caused an electrical current change, due to direct re-reduction of peroxidase by a direct electron transfer mechanism, which was measured to determine the herbicides in the sample. The optimal operational condition was found to be: $20\;{\mu}gl-1$ deglycosylated avidin loading to the working electrode and working potential +50 mV vs. Ag/AgCl. The total assay time was 15 min after sample addition. The detection limits for herbicides, atrazine and simazine, were found to be 3 ppb and 10 ppb, respectively.

Bioyield Strength and Ultimate Strength of Rough Rice (벼의 생물체(生物體) 강복강도(降伏强度) 및 극한강도(極限强度))

  • Kim, M.S.;Kim, S.R.;Park, J.M.;Myung, B.S.
    • Journal of Biosystems Engineering
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    • v.15 no.2
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    • pp.99-109
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    • 1990
  • Rough rice is subjected to a series of static and dynamic forces during mechanical harvesting, handling and processing operations. The mechanical properties such as bioyield point, compressive strength, and deformations at the bioyield point and rupture point are important engineering data needed to develop processing machines and to determine reasonable operating conditions for these machines. The objectives of this study were to determine the mechanical properties of the rough rice kernel at loading rate of 0.664 mm/min and 1.673 mm/min and at various moisture contents, and to examine the effect of the moisture content and the loading rate on these mechanical properties. The follwing results were obtained from the study. 1. Bioyield point, rupture point, bioyield strength and ultimate strength of the rough rice kernel generally decreased in magnitude with an increase in moisture content. A little larger values of these mechanical properties were obtained at the higher loading rate. The rough rice variety and the loading rate affected significantly these mechanical properties at low moisture content, but not at the higher moisture levels. 2. Bioyield point of the sample grains varied from 20 to 80 N, and rupture point varied from 45 to 130N. Bioyield point for Japonica-type rough rice was a little higher than that for Indica-type rough rice, but there were little differnces in rupture point between two types of rough rice. 3. Bioyield strength and ultimate strength of the Japonica-type rough rice varied from 10 MPa. to 39 MPa., and from 13 MPa. to 45 MPa. respectively. Those of the Indica-type rough rice varied from 12 MPa. to 42 MPa., and from 15 MPa. to 53 MPa. respectively. 4. Deformations at bioyield point and rupture point ranged from 0.18 mm/min to 0.26 mm, and from 0.28 mm to 0.53 mm respectively. These deformations decreased with an increase in moisture content up to moisture content of approximately 17% (w.b.) and increased again thereafter. 5. Regression equations were developed to predict these mechanical properties for the rough rice kernel as a function of moisture content.

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