Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Disposable Electrochemical Immunosensors for the Detection of Herbicide

제초제 검출을 위한 전기화학적 일회용 면역센서

  • Chang, Seung-Cheol (Institute of BioPhysio Sensor Technology (IBST), Pusan National University)
  • 장승철 (부산대학교 바이오피지오센서연구소)
  • Received : 2010.11.02
  • Accepted : 2010.12.15
  • Published : 2011.01.31
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Abstract

A disposable electrochemical immunosensor system has been developed for the detection of herbicide in aqueous samples. Disposable screen printed carbon electrodes(SPCE) were used as basic electrodes and an enzyme, horseradish peroxidase (HRP), and anti-herbicide antibodies was immobilised on to the working electrode of SPCE by using avidin-biotin coupling reactions. An herbicide-glucose oxidase conjugates have been used for the competitive immunoreaction with sample herbicides. The enzymatic reaction between the conjugated glucose oxidase and glucose added generates hydrogen peroxide, which was reduced by the peroxidase immobilised. The latter process caused an electrical current change, due to direct re-reduction of peroxidase by a direct electron transfer mechanism, which was measured to determine the herbicides in the sample. The optimal operational condition was found to be: $20\;{\mu}gl-1$ deglycosylated avidin loading to the working electrode and working potential +50 mV vs. Ag/AgCl. The total assay time was 15 min after sample addition. The detection limits for herbicides, atrazine and simazine, were found to be 3 ppb and 10 ppb, respectively.

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