• Title/Summary/Keyword: Sample-loading

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Electrical Property of Immobilized SWNTs Bundle as Bridge between Electrodes in Nanobiosensor Depending on Solvent Characteristics (시료용액의 특성에 따른 고정화된 단일벽 탄소나노튜브의 전기적 거동)

  • Lee, Jinyoung;Cho, Jaehoon;Park, Chulhwan
    • Korean Chemical Engineering Research
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    • v.55 no.1
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    • pp.115-120
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    • 2017
  • In recent, it is worldwide issued that nanoscale science and technology as a solution have supported to increase the sensing performance in carbon nanotube based biosensor system. Containing material chemistry in various nanostructures has formed their high potentials for stabilizing and activating biocatalyst as a bioreceptor for medical, food contaminants, and environmental detections using electrode modification technologies. Especially, the large surface area provides the attachment of biocatalysts increasing the biocatalyst loading. Therefore, nano-scale engineering of the biocatalysts have been suggested to be the next stage advancement of biosensors. Here, we would like to study the electrical mechanism depending on the exposure methods (soaking or dropping) to the sample solution to the assembled carbon nanotubes (CNTs) on the gold electrodes of biosensor for a simple and highly sensitive detection. We performed various experiments using polar and non-polar solutions as sampling tests and identified electrical response of assembled CNTs in those solutions.

Design Algorithm of Flexible Propeller by Fluid-Structure Interactive Analysis (유체-구조 반복해석법에 의한 유연 프로펠러의 설계 알고리듬 개발)

  • Jang, Hyun-Gil;Nho, In Sik;Hong, Chang-Ho;Lee, Chang-Sup
    • Journal of the Society of Naval Architects of Korea
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    • v.49 no.6
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    • pp.528-533
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    • 2012
  • Flexible composite propellers are subject to large deformation under heavy loading, and hence the hydrodynamic performance of deformed propeller might deviate from that of the metallic propeller under negligible deformation. To design the flexible propeller, it is therefore necessary to be able to evaluate the structural response of the blades to the hydrodynamic loadings, and then the influence of the blade deformation upon the hydrodynamic loadings. We use the lifting-surface-theory-based propeller analysis and design codes in solving the hydrodynamic problem, and the finite-element-method program formulated with 20-node iso-parametric solid elements for the analysis of the structural response. The two different hydrodynamic and structural programs are arranged to communicate through the carefully-designed interface scheme which leads to the derivation of the geometric parameters such as the pitch, the rake and the skew distributions common to both programs. The design of flexible propellers, suitable for manufacturing, is shown to perform the required thrust performance when deformed in operation. Sample design shows the fast iteration scheme and the robustness of the design procedure of the flexible propellers.

Research on Effects of Three Different Designs and Implementations on Cyber Education (정보활용기술 발전에 따른 효과적 사이버 교육을 위한 설계 및 구현의 차이에 대한 연구)

  • Ha, Tai-Hyun;Kang, Jung-Hwa
    • The Journal of Korean Association of Computer Education
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    • v.6 no.4
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    • pp.71-83
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    • 2003
  • This study is aimed to develop and evaluate different approaches for cyber education. The project involved the development of sample cyber education programs using different design approaches, with built-in evaluation mechanisms. The different design approaches depend on what delivery technologies are involved. In the First Generation, the delivery technologies use text, flash and animation, whereas the synchronized content to video and audio are used in the Second and the Third Generations but the difference is the delivery method used by the videoclip. Tests were carried out through self-assessment to measure and analyze the efficient teaching. The results show that the Third generation technologies were the most effective method for cyber education. However, since the Third generation program is developed in multimedia, it tends 10 require higher development costs, and more advanced hardware and software as well as a higher bandwidth for network. Therefore, the research indicates that the development of technical supports, like loading speed, has to be solved simultaneously with the development of multimedia products for effective cyber education.

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Comparison of polymer-based temporary crown and fixed partial denture materials by diametral tensile strength

  • Ha, Seung-Ryong;Yang, Jae-Ho;Lee, Jai-Bong;Han, Jung-Suk;Kim, Sung-Hun
    • The Journal of Advanced Prosthodontics
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    • v.2 no.1
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    • pp.14-17
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    • 2010
  • PURPOSE. The purpose of this study was to investigate the diametral tensile strength of polymer-based temporary crown and fixed partial denture (FPD) materials, and the change of the diametral tensile strength with time. MATERIAL AND METHODS. One monomethacrylate-based temporary crown and FPD material (Trim) and three dimethacrylate-based ones (Protemp 3 Garant, Temphase, Luxtemp) were investigated. 20 specimens (${\phi}\;4\;mm\;{\times}\;6\;mm$) were fabricated and randomly divided into two groups (Group I: Immediately, Group II: 1 hour) according to the measurement time after completion of mixing. Universal Testing Machine was used to load the specimens at a cross-head speed of 0.5 mm/min. The data were analyzed using one-way ANOVA, the multiple comparison Scheff$\acute{e}$ test and independent sample t test ($\alpha\;=\;0.05$). RESULTS. Trim showed severe permanent deformation without an obvious fracture during loading at both times. There were statistically significant differences among the dimethacrylate-based materials. The dimethacrylate-based materials presented an increase in strength from 5 minutes to 1 hour and were as follows: Protemp 3 Garant (23.16 - 37.6 MPa), Temphase (22.27 - 28.08 MPa), Luxatemp (14.46 - 20.59 MPa). Protemp 3 Garant showed the highest value. CONCLUSION. The dimethacrylate-based temporary materials tested were stronger in diametral tensile strength than the monomethacrylate-based one. The diametral tensile strength of the materials investigated increased with time.

A comparison of the implant stability among various implant systems: clinical study

  • Kim, Jae-Min;Kim, Sun-Jong;Han, In-Ho;Shin, Sang-Wan;Ryu, Jae-Jun
    • The Journal of Advanced Prosthodontics
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    • v.1 no.1
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    • pp.31-36
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    • 2009
  • PURPOSE. To determine the change in stability of single-stage, three different design of implant systems in humans utilizing resonance frequency analysis for early healing period(24 weeks), without loading. MATERIAL AND METHODS. Twenty-five patients were included into this study. A total of 45 implants, three different design of implant systems(group A,C,R) were placed in the posterior maxilla or mandible. The specific transducer for each implant system was used. ISQ(implant stability quotient) reading were obtained for each implant at the time of surgery, 3, 6, 8, 10, 12, 24 weeks postoperatively. Data were analyzed for different implant type, bone type, healing time, anatomical locations. RESULTS. For each implant system, a two-factor mixed-model ANOVA demonstrated that a significant effect on ISQ values(group A=0.0022, C=0.017, R=0.0018). For each implant system, in a two-factor mixed model ANOVA, and two-sample t-test, the main effect of jaw position(P > .005) on ISQ values were not significant. CONCLUSIONS. All the implant groups A, C and R, the change patterns of ISQ over time differed by bone type. Implant stability increased greatly between week 0 and week six and showed slow increase between week six and six months(plateau effect).

Verification with of High Efficiency Chemical Binding System of a Physiologically Active Radioisotope Using ESI-TOF/Ms System (고효율의 ESI-TOF/Ms 시스템을 이용한 생리활성 항체와 방사성동위원소 표지용 착화제의 결합 검증)

  • Joh, Eun-Ha;Hong, Young Don;Choi, Sun Ju
    • YAKHAK HOEJI
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    • v.57 no.6
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    • pp.400-405
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    • 2013
  • In this study, we measured the complex efficiency of a physiologically active antibody, a chelator and radiosiotopes using the ESI-TOF/Ms system for develop good radiopharmaceuticals. For a precise measurement, TLC is a low accuracy method. Loading of same amount of sample is difficult for each test, and work to quantify accurately the results obtained through TLC cannot be afforded compared to the use of other analytical instruments. The method of analysis using a mass spectrometer is capable of a mass analysis of proteins for quantitative analysis. The conjugates of the chelator (CHX-A- DTPA) and the antibody (IgG) were separated through MWCO, and were analyzed using ESI-TOF and MALDI-TOF mass spectrometry. The analysis using MALDI-TOF is roughly divided into measurements on mass spectrometry. When conjugating a small molecular weight of CHX-A-DTPA and a large molecular weight of IgG, distinguishing the peak of the conjugate and the peak of IgG was difficult. However, an ESI-TOF mass spectrometer system is capable of an analysis of mass by decentralizing the IgG. It is utilized as a technique for measuring the metabolic processes during conjugation and the stability evaluation of radiopharmaceuticals. When establishing this technique, the accuracy of the overall radiophar-maceutical analysis is expected to be able to be improved.

A Combination Model of Earthwork equipment using System Dynamics (시스템 다이내믹스를 활용한 토공장비의 조합 모형 연구)

  • Won, Seo-Kyung;Han, Choong-Hee;Kim, Sun-Kuk
    • Korean Journal of Construction Engineering and Management
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    • v.8 no.4
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    • pp.194-202
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    • 2007
  • The earth work has been improved with the utilization of capital intensive equipments. However, work performance is not satisfactory yet due to its experience-oriented characteristics in selecting the combination of earth work equipments. Therefore, it is required to study on appropriate and systematic methods in selecting a set of equipments for earth work in order to optimize the cost and time as well as to improve the productivity. The objectives of this paper are as followed : (1) developing an optimum combination model of earth work equipments by using a system dynamics technique. (2) testing and evaluating the model by the sample application to a case study. The research is limited to the work of excavating, loading and hauling. The suggested model is proved in practice, so it will assist engineers to make the proper decision for equipment planning of earth work.

Vulcanization Efficiency of Non-polar Rubber Compounds by Microwave (마이크로파를 이용한 비극성 고무컴파운드의 가황 효율)

  • Jung, U-Sun;Lee, Won-Ki;Lim, Kwon-Tack
    • Polymer(Korea)
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    • v.35 no.3
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    • pp.228-231
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    • 2011
  • The rate of vulcanization of nonpolar ethylene-propylene-diene terpolymer(EPDM)/carbon black compounds was investigated by using hot air and microwave as a heating source. The present study parameters such as heating source, sample thickness, and loading of an additive. The compound thickness was the main factor in the hot air vulcanization. It was due to the poor thermal conductivity of EPDM; that is, the thicker thickness, the lower vulcanization rate. For 100% vulcanization, the compound with 3 mm thickness required 7 min at $250^{\circ}C$ in the hot air system. However, the vulcanization of EPDM compounds by microwave system was not affected by the thickness while strongly dependent on the amount of a polar additive, carbon black. A compound with 80 phr of carbon black was perfectly vulcanized within 30 sec. These results suggest that the use of microwave as a heating source is an effective method for the vulcanization of compounds including a polar component.

Influence of ITO Thickness on the Deformation and Cracking Behaviors of ITO/PET Sheets (ITO층의 두께에 따른 ITO/PET sheet의 변형거동 및 균열 형성 거동)

  • Kim, Jin-Yeol;Hong, Sun-Ig
    • Korean Journal of Materials Research
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    • v.19 no.1
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    • pp.1-6
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    • 2009
  • In this study, the stress-strain response and the cracking behaviors of ITO film on a PET substrate are investigated. The cracking behaviors of ITO thin films deposited on a thermoplastic semi-crystalline polymer developed for flexible display applications was investigated by means of tensile experiments equipped with an electrical measurement apparatus and an in-situ optical microscope. Electrical resistance increased gradually in the elastic-to-plastic transition region of the stress strain curves and cracks formed. Numerous cracks were found in this region, and the increase of the resistance was linked to the cracking of ITO thin films. Upon loading, the initial cracks perpendicular to the tensile axis were observed at about 1% of the total strain. They propagated to the entire sample width as the strain increased. The spacing between the horizontal cracks is thought to be determined by the fracture strength and the thickness of the ITO film as well as by the interfacial strength between the ITO and PET. The effect of the strain rate on the cracking behavior was also investigated. The crack density increased as the strain increased. The spacing between the horizontal cracks (perpendicular to the stress axis) increased as the strain rate decreased. The increase of the crack density as the strain rate decreased can be attributed to the higher fraction of the plastic strain to the total strain at a given total strain. The higher critical strain for the onset of the increase in the resistance and the crack initiation of the ITO/PET with a thinner ITO film (300 ohms/sq.) suggests a higher strength of the thinner ITO film.

A Simple Purification of Apoliproteins A-I and B and Their Application to Cholestery Ester Transfer Assay

  • Cho, Kyung-Hyun;Park, Myung-Sook;Bok, Song-Hae;Park, Young-Bok
    • Preventive Nutrition and Food Science
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    • v.1 no.1
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    • pp.87-92
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    • 1996
  • This study describes a stable and simple method for the measurement of cholesteryl ester transfer protein(CETP) activities using reconstituted HDL and LDL as substrates. Apolipoproteins (apo) A-I and -B were purified from hog plasma by a new strategy without ultracentrifugation and delipidation. a simple two-step column chromatography was administered. In the first step of phenyl-sepharose CL-4B column chro-matography, hydrophobic plasma proteins were isolated. The most hydrophobic proteins bound to the column appeared to be A-I and apo-B. Contaminat proteins were efficiently eliminated from the sample by washing the column with 0.3M NaCI containing buffer after loading the plasma on the column. Two pure proteins showing each single band on SDS-PSGE of apo A-I and apo-B were individually obtained by a subsequent gel filtration column chromatography(Sephadex G-200). This two-step purification was simple and inexpensive compared to the ultracentrifugation and/or delipidation method that are most commonly used. Reconstituted hight-density lipoproteins(HDL) and low-density lipoproteins(LDL) were prepared using the purified apo A-I and-B, respectively. When these artificially prepared HDL and LDL were used in the assays for CETP as the cholesteryl ester(CE) donor and acceptor respectively, the specific transfer of CE increased up to two fold compared to that used the native HSL and LDL.

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