• Korean Journal of Pharmacognosy
• /
• v.30 no.2
• /
• pp.158-162
• /
• 1999

The rhizomes of Salvia miltiorrhiza Bunge(Labiatae) has been used in Chinese traditional medicine for the treatment of coronary heart diseases and myocardial infarction. As a part of a research for standardization of crude oriental drugs, we have determined the content of tanshinone IIA in Salvia miltiorrhiza purchased from various regions of Korea. The HPLC method by which quantitative analysis was conducted, showed reproducible results and chromatographic isolation of tanshinone IIA was accomplished successively.

• Korean Journal of Food Science and Technology
• /
• v.39 no.1
• /
• pp.83-87
• /
• 2007

Salvia miltiorrhiza Bunge is known to potentially prevent arteriosclerosis and hypertension, but its effects on platelet function are not clear. In this study, we evaluated the in vitro antithrombotic activities of the edible plant extract. Methanol extract of S. miltiorrhiza Bunge exhibited about 70% fibrinolytic activity compared to the plasmin control, and inhibited ADP- and collagen-induced platelet aggregation in a concentration-dependent manner with $IC_{50}$ values of 0.42 and 0.07 mg/mL, respectively. S. miltiorrhiza Bunge extract significantly prolonged the activated partial thromboplastin time (APTT) and prothrombin time (PT) compared with control. Moreover, 0.05 mg/mL S. miltiorrhiza Bunge extract contained 87.3% l,l-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity. In conclusion, S. miltiorrhiza Bunge seemed to enhance antithrombotic activity due to its radical scavenging activity. Based on these data, further examination is required to determine the mechanism of platelet-dependent antithrombosis and the effect of polyphenols on platelet function.

• Korean Chemical Engineering Research
• /
• v.46 no.4
• /
• pp.660-664
• /
• 2008

In this work, analytical HPLC was utilized to obtain Tanshinone IIA (TIIA) from Salvia Miltiorrhiza Bunge (SMB). The optimum operating conditions were experimentally determined to analyze the TIIA in the pretreated extract. SMB was extracted with the various organic solvents of methanol, ethyl acetate, and ethanol, then the extract was analyzed to compare the amount of TIIA. From the results, the methanol showed the best extraction efficiency of TIIAd. The analysis by $C_{18}$ column was performed. The mobile phase was composed of methanol and water, and the isocratic elution mode was mainly applied. $2.154{\mu}g$ of TIIA/mg of SMB powder was extracted with methanol.

• Korean Journal of Pharmacognosy
• /
• v.49 no.4
• /
• pp.322-327
• /
• 2018

Methanol extract of Salvia miltiorrhiza Bunge (Labiatae) root was investigated to research the anti-oxidative activity, by using a Caenorhabditis elegans model system. The methanol extract of this plant showed significant DPPH radical scavenging and superoxide quenching activities. Ethyl acetate soluble fraction of the methanol extract that showed the most potent DPPH radical scavenging and superoxide quenching activities. The ethyl acetate fraction was tested on its activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance in C. elegans. Furthermore, in order to see if regulation of stress-response genes is responsible for the increased stress tolerance of the ethyl acetate fraction treated C. elegans, we checked SOD-3 expression using a transgenic strain. Consequently, the ethyl acetate fraction of S. miltiorrhiza root increased the catalase and SOD activities in a dose-dependent manner in C. elegans. Besides, the ethyl acetate fraction-treated CF1553 worms showed higher SOD-3::GFP intensity than the non-treated ones.

• Archives of Pharmacal Research
• /
• v.28 no.8
• /
• pp.909-913
• /
• 2005

We screened natural products to find compounds with anti-osteoporotic potential using a coculture-based system by which osteoclast differentiation is effectively achieved. We found that methylene chloride soluble fraction of the root of Salvia miltiorrhiza Bunge (Labiatae) suppressed osteoclast differentiation. Five tanshinones, tanshinone IIA (1), tanshinone I (2), cryptotanshinone (3), 15,16-dihydrotanshinone I (4), and ferruginol (5) were subsequently isolated from fraction. Among the five compounds, compounds 1-4 reduced the formation of TRAP­positive multinuclear osteoclasts. These results suggest that the identified tanshinones may be useful candidates for development of therapeutic agents to treat osteoporosis and other bone-resorptive diseases.

• Korean Journal of Pharmacognosy
• /
• v.42 no.3
• /
• pp.265-270
• /
• 2011

Acute toxicity of a combined preparation of the standardized extracts Scutellaria baicalensis GEORGI and Salvia miltiorrhiza BUNGE in a ratio of 3:1 was examined in male and female ICR mice. Mice were treated with the test substance intragastrically at a dose of 0 mg/kg, 5 mg/kg, 50 mg/kg, 500 mg/kg or 2,000 mg/kg and observed for two weeks. No death or abnormal clinical sign was shown during the observation period. Also there were no difference in net body weight gain, organ weight, and gross pathological findings at the terminal sacrifice. The results suggested that acute oral toxicity of a combined preparation of the standardized extracts is very low at the conditions employed in this study.

• Korean Journal of Microbiology
• /
• v.40 no.2
• /
• pp.111-114
• /
• 2004

This study was carried out to evaluate the inhibition of growth and collagenase activity of Salvia miltiorrhiza Bunge against microorganisms causing periodontal diseases. The ethanol extract of Salvia miltiorrhiza showed sig-nificant growth inhibition against microorganisms causing periodontal diseases. Ethanol extract was further fractionated with organic solvents in the order of hexane, chloroform and ethyl acetate. Among the fractions tested, the hexane fraction showed the highest cell growth inhibition. The minimal inhibitory concentration (MIC) of the extract against C. curvus, C. rectus, E. corrodens, F nucleatum, P. gingivalis, P. intermedia and W. succinogenes were 200, 50, 50, 250, 150, 250 and 200 ${\mu}g$/ml, respectively. The inhibition of collagenase activity by organic solvent fractions were higher than that of minocycline, and the inhibition ratio of collagenase activity was $88.2{\pm}2.1$ % in the chloroform fraction.

• Journal of Microbiology
• /
• v.45 no.4
• /
• pp.350-357
• /
• 2007

This study was conducted in an effort to evaluate the antimicrobial activity and antibiotic-resistant gene regulation from Saliva miltiorrhiza Bunge on methicillin-resistant Staphylococcus aureus (MRSA). A variety of solvent fractions and methanol extracts of S. miltiorrhiza Bunge were tested in order to determine its antimicrobial activities against S. aureus and MRSA. As a result, the hexane fraction of S. miltiorrhiza Bunge evidenced the highest levels of antimicrobial activity against S. aureus and MRSA. The MICs of the hexane fraction against various MRSA specimens were $64. The hexane fraction evidenced inhibitory effects superior to those of the chloroform fraction. The results showed inhibition zones of hexane (16 mm) and chloroform (14 mm) fractions against MRSA KCCM 40511 at $1,000{\mu}g/disc$. The hexane and chloroform fractions inhibited the expression of the resistant genes, mecA, mecR1, and femA in mRNA. Moreover, the results of Western blotting assays indicated that the hexane and chloroform fractions inhibited the expression of the resistant protein, PBP2a. These results reveal that the hexane and chloroform fractions of S. miltiorrhiza Bunge may prove to be a valuable choice for studies targeted toward the development of new antimicrobial agents.

• Korean Journal of Medicinal Crop Science
• /
• v.28 no.3
• /
• pp.200-208
• /
• 2020

Background: Salvia miltiorrhiza Bunge has been used in traditional medicine. The type of light source has an effect on the growth properties and composition of functional compounds in plants. In this study, we analyzed the effects of different artificial light sources on the growth characteristics as well as antioxidant and antimicrobial activities of S. miltiorrhiza. Methods and Results: Seedlings of S. miltiorrhiza were grown under various artificial light sources, including fluorescent light (FL), light emitting diode (LED), and microwave electrodeless light (MEL), for 8 weeks. Growth characteristics were the best in plants treated with MEL. DPPH scavenging activity of the shoot was more pronounced with the FL treatments, while the roots were more active in plants grown under single wavelength lights (i.e., blue and red LEDs). Among the different light source treatments, the blue LED resulted in a higher total phenolic content in the plants. Furthermore, growing plants growth under the red LED enhanced their total flavonoid content. Notably, the antimicrobial properties of plants varied significantly between light source treatments in this study. Except for E. coli, all the tested microorganisms were susceptible to the plant extracts. Conclusions: The type of light source may be an important parameter for the enhancement of plant growth and functional compounds in S. miltiorrhiza.

• Microbiology and Biotechnology Letters
• /
• v.38 no.3
• /
• pp.289-294
• /
• 2010

Salvia miltiorrhiza Bunge (S. miltiorrhiza) is a traditional Korean medicine that is commonly used for the treatment of inflammatory diseases such as edema, arthritis, and hepatitis. The present study investigated the antimicrobial activity of methanol (MeOH) extract of S. miltiorrhiza roots against oral bacteria using broth the microdilution method and the checkerboard and time-kill methods evaluated the synergistic effects of treatment with antibiotics. The MeOH extract was demonstrated as a higher antibacterial activity (MICs, 8 to $64\;{\mu}g/mL$; MBCs, 16 to $64\;{\mu}g/mL$) against all tested oral bacteria. Additionally, the extract was observed to have a synergistic effect with ampicillin or gentamicin. A time-kill study evaluating the effects of the extract indicated that the extract treatment in combination with ampicillin or gentamicin showed rapid bactericidal activity. The results suggest that MeOH extract of S. miltiorrhiza could be employed as a natural antibacterial agent against dental caries.