• The Journal of Advanced Prosthodontics
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• v.12 no.4
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• pp.204-209
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• 2020

PURPOSE. Prevention of xerostomia and stress is important to prolong healthy life expectancy and improve the quality of life. We aimed to investigate the effects of tongue rotation exercise for increasing salivary secretions and stabilizing salivary stress hormone levels. MATERIALS AND METHODS. Twenty four participants without subjective oral dryness were enrolled. The exercises comprised tongue rotation exercise and empty chewing. The salivary stress hormone level was measured using a Salivary Amylase Monitor. Unstimulated whole saliva volume and salivary amylase activity were measured before tongue rotation exercise or empty chewing and subsequently 5, 10, and 15 minutes after these exercises. Differences in the rates of change of unstimulated whole saliva volume and salivary amylase activity were analyzed by repeated measure analysis of variance. RESULTS. Statistically significant differences among the rates of change were not observed after empty chewing for unstimulated whole saliva volume and salivary amylase activity at the four measurement times. However, the rate of change of unstimulated whole saliva volume and salivary amylase activity were statistically significantly different among the four time points: before the tongue rotation exercise and 5, 10, and 15 minutes post-exercise (P<.05 and P<.01, respectively). CONCLUSION. Tongue rotation is effective in increasing saliva secretion, reducing stress, improving oral function, and extending healthy life expectancy.

• Journal of the Health Care and Life Science
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• v.10 no.1
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• pp.163-167
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• 2022

Water is an indispensable material for life and health, an essential ingredient for maintaining blood volume, and plays an important role in nutrient supply and waste excretion in the body. Water is also involved in hormone secretion, and when the body dehydration, the main organs cannot function and secrete stress hormones. Therefore, we investigated the changes in salivary cortisol hormone after water intake in 32 healthy adults. They were divided into two groups: The control group (N=16), which consumed water ad libitum, and the experimental group (N=16), which consumed 2L of water per day. Of blood biochemical parameters, a significant decrease in the concentration salivary cortisol hormone decreased in the experimental group. (p < 0.05). In conclusion, a steady and sufficient water intake may contribute to alleviate stress by decreasing cortisol hormone. As a result, constant intake of water appears to reduce the secretion of stress hormones by leading organs functioning properly, further improving immune function.

• The Journal of the Korean dental association
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• v.54 no.11
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• pp.897-906
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• 2016

Objectives: Periodontitis is multifactorial disease mainly caused by microbial community. Recently, some research has been conducted to find other possible risk factors including stress hormones related to periodontitis. Psychological stress can affect the periodontal health by a variety of biological mechanisms. This study compared the stress hormone levels in healthy subjects and patients with periodontal disease using saliva in order to investigate the association between periodontitis and stress. Methods: The human saliva was collected from 38 periodontally healthy individuals and 34 patients with chronic periodontitis under Institutional Review Board. Their age was 20-60 years ($40.3{\pm}10.45$). From these samples, determination of salivary levels of cortisol and Dehydroepiandrosterone (DHEA) performed by enzyme immunoassay kit (Salimetrics Europe, Suffolk, UK). The independent t-test and Mann-Whitney test for trend was applied using IBM SPSS statistics version 12.0 Program to analyze statistically significant differences. Results: Salivary cortisol levels of periodontitis patients were higher than those levels of healthy subjects (P < 0.001), while salivary DHEA levels of periodontitis patients were not significantly different (P = 0.431). Salivary cortisol/DHEA ratio of periodontitis patients was higher than those levels of healthy subjects (P < 0.001). Conclusions: Our study demonstrates the high levels of cortisol concentrations and cortisol/DHEA ratio in saliva of periodontitis patients than those of healthy subjects. Since cortisol levels and cortisol/DHEA ratio can be significant factors related to the severity of periodontal disease, our study would be helpful for early diagnosis and treatment of periodontal disease.

• Proceedings of the SCSK Conference
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• 2003.09b
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• pp.456-457
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• 2003

The use of relaxing cosmetic products is more and more widespread on the self care market, since stress is considered as a major cause of discomfort in modem life. Cortisol, the stress hormone, helps the human body to regulate itself by acting on carbonhydrate, proteins and fat metabolisms. IgA are immune molecules involved in the stress response of the human body. The less the human body is stressed, the more the salivary IgA rate increases. Salivary cortisol and IgA have the advantage of being easily assessed with non invasive methods. Finally, conductance is the measurement of the modification in the electrical resistance of the skin. By this means, the measurement of the conductance enable the assessment of somebody's stress.(omitted)

• Journal of Oral Medicine and Pain
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• v.32 no.2
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• pp.201-210
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• 2007

Many diseases occur by stress or effect of stress. The basis for using hormones in research of stress is the observation that most systems in the body show changes during stress and that hormonal markers in these changes are related with stress. Conceptually, the central role of sympathetic nervous system(SNS) and hypothalamus-pituitary-adrenal(HPA) axis activity in stress provides copious justification for measuring hormonal changes. Catecholamines like epinephrine and norepinephrine, cortisol, testosterone and growth hormone and so on show sensitive reaction to stress. The major advantage of salivary sample to stress research is that its sampling technique can be performed in non-stressful conditions and without physical restraint and ethical problems. Because hormone levels in saliva is a good reflection of hormone levels in plasma, application of stress hormones in saliva is very useful for research of orofacial pain related with stress.

• Journal of The Korean Association For Science Education
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• v.29 no.7
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• pp.783-791
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• 2009

This study was conducted to examine my hypothesis that how teacher's teaching style influences emotional and physiological states of students in the secondary school science classroom. Sixty healthy secondary school students were participated in this study and divided into two groups: manipulation and non-manipulation. Each group underwent different styles of teaching on the scientific hypothesis-generating of com starch experiment. Before and after the class, the strength of emotion was measured using adjective emoticon check lists and they extracted their saliva sample for salivary hormone analysis. Here are the results of this study. First, the intensity of positive emotions in the manipulation group was significantly stronger than the one in the non-manipulation group, whereas the intensity of negative emotions in the non-manipulation group was significantly stronger than the one in the manipulation group. Second, the cortisol level, an indicator of stress, was decreased in the manipulation group whereas it was increased in non-manipulation group. Third, the quality of scientific hypotheses which is generated by students during the class had no connection with types of instructions. Fourth, this study found significantly negative correlation between students' emotional intensity of interest and concentration changes of salivary cortisol. Therefore, the different teaching styles have influence upon students' attitude and interest in science.

• Journal of Fashion Business
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• v.17 no.6
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• pp.18-27
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• 2013

This study is being conducted in order to confirm the effects of inhaling method for aromatherapy on cortisol which is a stress hormone by the saliva test method. An attempt is being made to dertermine if there are any differences between the effect of lavender and rosemary when concidering their effects on the cortisolsl. The test shows a statistically significant decrease in the cortisol levesl(Table 4), and as far as the comparison of the effects between lavender(N=10) and rosemary(N=10) is concerned, the lavender group show a p-value of .005 which means that there are no statistical significances; while the rosemary group show p-values of .081 meaning that there is a decrease in cortisol levels, which is statistically significant(Table 5). It has been proven that the saliva test method is a practical and scientific method when confirming the effects of aromatherapy and also a convenient method for both of the test coordinator and the subjects. Results of all 20 subjects showed similar results obtained by means of conventional blood tests. However, the rosemary group shows statistically significant decrease in cortisol level compared to the lavender group, thus proving that the test method for studying the effect of aromatherapy on stress is valid. Further studies should be conducted in order to investigate the differences in the effects of the cortisol level at different concentrations of the aroma.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.5
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• pp.734-741
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• 2019

Objective: The objective of the study was to determine the relationship between plasma and salivary cortisol concentrations in beef cattle that were subjected to handling prior to sampling. Methods: Twenty-one Nguni cows of three age categories; 5 to 7 yr (n = 7), 8 to 10 yr (n = 6), and 11 to 13 yr (n = 8) were handled for five consecutive weeks. In the pen, a human avoidance test was performed and cattle responses to restraint in the chute and crush were observed. In addition, rectal temperature readings were taken and, faecal samples were collected and analysed for glucocorticoid metabolites. Through the handling and restraint process, excretory and vocalisation behaviour, as a sign of stress were observed and recorded. Thereafter, six cows were randomly selected and subjected to an adrenocorticotropic hormone (ACTH) challenge. Blood and saliva samples were extracted to determine cortisol concentrations. Results: Repeated handling affected (p<0.05) faecal glucocorticoid metabolites, rectal temperatures, avoidance distance, crush scores as well as urination and defaecation behaviour. Acclimation to handling was variable based on each respective parameter. Saliva cortisol concentrations increased and decreased significantly (p<0.001). A peak value of $136.78{\pm}15.869nmol/L$ was observed 30min after administration of ACTH, from a baseline value of $8.75{\pm}15.869nmol/L$. Plasma cortisol concentrations did not differ (p>0.05) across the time of sampling. A low and insignificant correlation (r = 0.0131, p>0.05) between plasma and saliva cortisol was therefore observed. Conclusion: We conclude that if beef cows are subjected to handling prior to sampling, a weak relationship exists between plasma and salivary cortisol levels.

• Journal of People, Plants, and Environment
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• v.23 no.4
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• pp.411-422
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• 2020

Background and objective: This study was conducted to find out the effects of healing farm resources and participant satisfaction on the level of stress and loyalty. Methods: The experiment was conducted on 18 subjects who participated in agro-healing activities (aged 49.5 years on average) and the concentration of cortisol, the level of stress and satisfaction with the services and program of a healing farm. The healing-agriculture activities were performed once a week, a total of 7 sessions, for 90 minutes and their saliva was collected before and after the activities. Results: The concentration of cortisol, a stress hormone, was statistically significantly reduced after the healing-agriculture activities in 5 out of 7 sessions. The total cortisol concentration showed statistically significant differences. The 6th session was comprised of different activities, compared to others, such as understanding weeds, managing a garden and making a pizza with fermentation enzymes, and the level of cortisol in the 6th session was the lowest after participating in the agro-healing activities, which indicated that the activities have the positive effect of lowering the level of stress. The level of stress was lowered from 18.39 to 16.78, which was consistent with the results of the concentration of cortisol. The level of satisfaction in the last session was below 50%, which indicated that the owner of the farm might be a little inexperienced in education and marketing on information and safety. The variables of participants including stress and satisfaction with services showed a strong influence on their loyalty. Conclusion: Satisfaction with services and loyalty to the healing farm showed a very high correlation, which was statistically significant. It also showed that participants' stress level and satisfaction with the services and program of the healing farm have a strong influence on their loyalty.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.10
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• pp.471-479
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• 2017

The purpose of this study was to investigate changes in salivary cortisol content in 35 subjects before and after visiting Gotjawal Forest in Jeju. Cortisol raw samples were collected before and after visiting Gotjawal Forest and were analyzed by salivary cortisol enzyme immunoassay. The period of the study was from May 1, 2017 to June 30, 2017. There was no significant difference in cortisol content between the control and experimental groups before the visit, but the value of the experimental group significantly decreased afterwards. The mean value of cortisol in male subjects was lower than that of the control group. In the case of women, the value of the experimental group was lower than that of the control group. The post-visit cortisol content of the experimental group was significantly lower than that of the control group. In the post-visit analyses according to gender and group, there were significant differences between genders, groups, and combined effects of gender and group. This study provides basic data to prove the effects of forest bathing.