• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1672-1683
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• 2021

Vibrio parahaemolyticus is recognized as one of the most important foodborne pathogens responsible for gastroenteritis in humans. The bla_CARB-17 gene is an intrinsic β-lactamase gene and a novel species-specific genetic marker of V. parahaemolyticus. In this study, a loop-mediated isothermal amplification (LAMP) assay combined with a lateral flow dipstick (LFD) was developed targeting this bla_CARB-17 gene. The specificity of LAMP-LFD was ascertained by detecting V. parahaemolyticus ATCC 17802 and seven other non-V. parahaemolyticus strains. Finally, the practicability of LAMP-LFD was confirmed by detection with V. parahaemolyticus-contaminated samples and natural food samples. The results showed that the optimized reaction parameters of LAMP are as follows: 2.4 mmol/l Mg²⁺, 0.96 mmol/l dNTPs, 4.8 U Bst DNA polymerase, and an 8:1 ratio of inner primer to outer primer, at 63℃ for 40 min. The optimized reaction time of the LFD assay is 60 min. Cross-reactivity analysis with the seven non-V. parahaemolyticus strains showed that LAMP-LFD was exclusively specific for V. parahaemolyticus. The detection limit of LAMP-LFD for V. parahaemolyticus genomic DNA was 2.1 × 10^-4 ng/μl, corresponding to 630 fg/reaction and displaying a sensitivity that is 100-fold higher than that of conventional PCR. LAMP-LFD in a spiking study revealed a detection limit of approximately 6 CFU/ml, which was similar with conventional PCR. The developed LAMP-LFD specifically identified the 10 V. parahaemolyticus isolates from 30 seafood samples, suggesting that this LAMP-LFD may be a suitable diagnostic method for detecting V. parahaemolyticus in aquatic foods.

• Microbiology and Biotechnology Letters
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• v.49 no.4
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• pp.510-518
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• 2021

Bacteriophages are considered excellent sensing elements for platforms detecting bacteria. However, their lytic cycle has restricted their efficacy. Here, we used the minor coat protein pIII domain (N1N2) of phage CTXφ to construct a novel surface plasmon resonance (SPR) biosensor that could detect Vibrio cholerae. N1N2 harboring the domains required for phage adsorption and entry was obtained from Escherichia coli using recombinant protein expression and purification. SDS-PAGE revealed an approximate size of 30 kDa for N1N2. Dot blot and transmission electron microscopy analyses revealed that the protein bound to the host V. cholerae but not to non-host E. coli K-12 cells. Next, we used amine-coupling to develop a novel recombinant N1N2 (rN1N2)-functionalized SPR biosensor by immobilizing rN1N2 proteins on gold substrates and using SPR to monitor the binding kinetics of the proteins with target bacteria. We observed rapid detection of V. cholerae in the range of approximately 10³ to 10⁹ CFU/ml but not of E. coli at any tested concentration, thereby confirming that the biosensor exhibited differential recognition and binding. The results indicate that the novel biosensor can rapidly monitor a target pathogenic microorganism in the environment and is very useful for monitoring food safety and facilitating early disease prevention.

• Journal of The Korea Institute of Healthcare Architecture
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• v.28 no.2
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• pp.31-38
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• 2022

Purpose: This study presents the results of the analysis on space utilization of kidney dialysis units in regional public hospitals, which plays a key role in local public medical services. The result aims to achieve safety from infection, allow comfort for the dialysis environment, and stability for medical support. The purpose of this study is to present fundamental data for architectural plans for the kidney dialysis unit, as well as to alleviate potential infectious diseases such as COVID-19. Methods: For research purposes, the investigation and analysis of space utilization were based on architectural floor plans, research papers and literature, related legal systems, and public statistics. Of the main 35 regional public hospitals, in regards to data accessibility, 15 facilities were selected to conduct the survey and analysis for the objective. Results: The space composition by area research results of kidney dialysis units in public hospitals are as follows: Firstly, most targets do not have required rooms in the access and support area, except for the hemodialysis beds in the treatment section. Secondly, the access area requires necessary room and space design that took into consideration of convenience and accessibility for patients. Thirdly, in regards to infection prevention and control, proper circulation and room plan is essential for storage and disposal of contaminated products and linen after use. For the treatment area, the arrangement plan needs to establish a visual connection between the isolation room, the nursing station, and the bed area. Additionally, consideration of circulation in the preparation, treatment, observation, examination, and all other rooms in the facility is required. Lastly, for the support area, the room is designed to consider adequate working and meeting spaces for the medical staff, consultation space for patients or guardians, separate storage and disposal of clean and contaminated items, and the storage of various equipment for dialysis. Implications: In preparation for the increase in chronic kidney failure patients and the spread of infectious diseases, such as COVID-19, the researched data demonstrates the basic guidelines for space composition of kidney dialysis units and the significant role of regional public hospitals.

• Korean Journal of Veterinary Service
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• v.44 no.4
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• pp.283-290
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• 2021

This is a study on the improvement of the chemical treatment method of the livestock carcass treatment newly introduced in the livestock infectious disease prevention method in order to improve the problems of the existing burial-centered carcass treatment method when a livestock infectious disease occurs. It was conducted to establish detailed treatment standards for the chemical treatment method of pig carcasses based on the results of proof of the absence of infectious diseases in pigs. After inoculating pig carcasses with 10 pathogens (6 viruses [FMDV, ASFV, CSFV, PCV2, PRRSV, PEDV] and 4 bacteria [Lawsonia intracellularis, Clostridium perfringens type C, E. coli, Salmonella Typhimurium]) It was treated at 90℃ for 5 hours in a potassium hydroxide (KOH) liquid solution corresponding to 15% of the body weight. This method liquefies all cadaveric components and inactivates all inoculated pathogens. Based on these results, it was possible to prove that chemical treatment of pig carcasses is effective in killing pathogens and is a safe method without the risk of disease transmission. Although there are problems to be solved in the processing and operation of the chemical treatment products of livestock carcasses, the chemical treatment method of livestock carcasses can be suggested as an alternative to the current domestic burial-centered livestock carcass treatment method, preventing environmental pollution, and contributing to public health.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.22 no.2
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• pp.51-62
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• 2022

This study suggests a design of predictive modeling for a hospital fall risk based on inpatients' posture. Inpatient's profile, medical history, and body measurement data along with basic information about a bed they use, were used to predict a fall risk and suggest an algorithm to determine the level of risk. Fall risk prediction is largely divided into two parts: a real-time fall risk evaluation and a qualitative fall risk exposure assessment, which is mostly based on the inpatient's profile. The former is carried out by recognizing an inpatient's posture in bed and extracting rule-based information to measure fall risk while the latter is conducted by medical staff who examines an inpatient's health status related to hospital fall risk and assesses the level of risk exposure. The inpatient fall risk is determined using a sigmoid function with recognized inpatient posture information, body measurement data and qualitative risk assessment results combined. The procedure and prediction model suggested in this study is expected to significantly contribute to tailored services for inpatients and help ensure hospital fall prevention and inpatient safety.

• Journal of Convergence for Information Technology
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• v.12 no.4
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• pp.1-8
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• 2022

With the development of communication infrastructure, the number of network equipment owned by one person is gradually increasing. General-purpose devices such as smartphones can implement theft/loss prevention function by implementing S/W. However, other small devices lack practicality such as long-distance communication problems due to standard communication technology specifications or H/W limitations, and lack of functions(authentication and security). This study combines the Lora communication protocol in the LPWA standard environment and the blockchain technology. Anti-theft and security functions were added to the protocol, and the PBFT consensus algorithm was applied to build a blockchain network. As a result of the test, the effectiveness of safety(authentication and trust network) and performance(blockchain processing performance) were confirmed. This study aims to contribute to the future development of portable or small device anti-theft products as a 4th industrial convergence research.

• Earthquakes and Structures
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• v.22 no.6
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• pp.561-570
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• 2022

It is of great importance to be able to evaluate different structural systems not only based on their seismic performance but also considering their lifetime service costs. Many structural systems exist that can meet the engineering requirements for different performance levels; therefore, these systems shall be selected based on their economic costs over time. In this paper, two structural systems, including special steel moment-resisting and the ordinary concentric braced frames, are considered, which are designed to meet the three performance levels: Immediate Occupancy (IO), Life Safety (LS), Collapse Prevention (CP). The seismic behavior of these two systems is studied under three strong ground motions (i.e., Tabas, Bam, Kajour earthquake records) using the Perform3D package, and the incurred damages to the studied systems are examined at two hazard levels. Economic analyses were performed to determine the most economical structural system to meet the specified performance level requirements, considering the initial cost and costs associated with damages of an earthquake that occurred during their lifetime. In essence, the economic lifetime study results show that the special moment-resisting frames at IO and LS performance levels are at least 20% more economical than braced frames. The result of the study for these building systems with different heights designed for different performance levels also shows it is more economical from the perspective of long-term ownership of the property to design for higher performance levels even though the initial construction cost is higher.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.26 no.8
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• pp.1231-1237
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• 2022

It is necessary to dynamic recognition system with real time loading height and pressure of the loading waste, the drying of wood, batteries, and plastic wastes, which are representative compositional wastes, and the carbonization changes on the surface. The dynamic context awareness service constituted a platform based on Universal Middleware system using BCN convergence communication service as a Ambient SDK model. A context awareness system should be constructed to determine the cause of the fire based on the analysis data of fermentation heat point with natural ignition from the load waste. Furthermore, a real-time dynamic service platform that could be apply to the configuration of scenarios for each type from early warning fire should be built using Universal Middleware. Thus, this issue for Internet of Things realize recognition platform for analyzing low temperature fired fire possibility data should be dynamically configured and presented.

• The Journal of Engineering Geology
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• v.31 no.1
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• pp.19-30
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• 2021

This study evaluated the injection rate and the injection efficiency of the artificial recharge in the upper drought-prone watershed region, where the remaining water was used for injection, by using a numerical model to secure water during a drought. As a result of a numerical model under the condition of diverse injection rates per a well and hydraulic characteristics of the aquifer, the optimal injection rate per a well was estimated as 50.0 ㎥/day, and the injection efficiency was simulated as 33.2% to 81.2% of the total injection volume. As the injection time was shorter, the injection efficiency tented to increase non-linearly. As the injection rate increased, the residual storage in aquifer increased and available groundwater amount also increased, which could be advantageous for drought relief. For a more accurate assessment of injection efficiency, the model will be validated using the field injection data and optimum scenarios will enable the efficient operation of the artificial recharge system in the study area.

• Journal of Navigation and Port Research
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• v.45 no.3
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• pp.95-101
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• 2021

Marine Incidents based on Heinrich's law are very important in preventing accidents. However, marine Incident data are mainly qualitative and are used to prevent similar accidents through case sharing rather than statistical analysis, which can be confirmed in the marine Incident-related data posted in the Korea Maritime Safety Tribunal. Therefore, this study derived quantitative results by analyzing the causes of marine incidents during berthing using various methods of statistical analysis. To this end, data involving marine incidents from various shipping companies were collected and reclassified for easy analysis. The main keywords were derived via primary analysis using text mining. Only meaningful words were selected via verification by an expert group, and time series and cluster analysis were performed to predict marine incidents that may occur during berthing. Although the role of an expert group was still required during the analysis, it was confirmed that quantitative analysis of marine incidents was feasible, and iused to provide cause and accident prevention information.