• 대한약침학회지
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• 제18권3호
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• pp.63-67
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• 2015

Objectives: This study was performed to analyze the single-dose oral toxicity of the super key (processed sulfur). Methods: All experiments were conducted at Medvill, an institution authorized to perform non-clinical studies, under the Good Laboratory Practice (GLP) regulations. In order to investigate the oral toxicity of super key. We administered it orally to Sprague-Dawley (SD) rats. The SD rats were divided into four groups of five male and five female animals per group: group 1 being the control group and groups 2, 3, and 4 being the experimental groups. Doses of super key 500 mg/kg, 1,000 mg/kg and 2,000 mg/kg were administered to the experimental groups, and a dose of normal saline solution, 10 mL/kg, was administered to the control group. We examined the survival rates, weights, clinical signs, gross findings and necropsy findings. This study was conducted under the approval of the Institutional Animal Ethics Committee. (Approval number: A01-14018). Results: No deaths or abnormalities occurred in any of the four groups. Although slight decreases in the weights of some female rats were noted, no significant changes in weights or differences in the gross findings between the control group and the experimental groups were observed. To check for abnormalities in organs, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs. Conclusion: The results of this research showed that administration of 500 - 2,000 mg/kg of super key did not cause any changes in the weights or in the results of necropsy examinations. Neither did it result in any mortalities. The above findings suggest that treatment with super key is relatively safe. Further studies on this subject are needed to yield more concrete evidence.

• 대한의용생체공학회:의공학회지
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• 제34권1호
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• pp.14-23
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• 2013

In this study, biomechanical stability of the newly developed revision total knee arthroplasty (rTKA) was evaluated through strain and stress distribution analysis within the implanted proximal tibia using a three-dimensional finite element (FE) analysis. 2000N of compressive load (about 3 times body weight) was applied to the condyle surface on spacer, sharing by the medial (60%) and lateral (40%) condyles simulating a stance phase before toe-off. The results showed that PVMS within the revision total knee arthroplasty and the proximal tibia were less than yield strength considering safe factor 4.0 (rTKA: less than 10%, Cortical bone: less than 70%, Cancellous bone: less than 70%). The materials composed of them and the strain and stress distributions within the proximal tibia were generally well matched with those of a traditional revision total knee arthoplasty (Scorpio TS revision system, Stryker Corp., Michigan, USA) without the critical damage strain and stress, which may reduce the capacity for bone remodeling, leading to bone degeneration. This study may be useful to design parameter improvement of the revision total knee arthoplasty in biomechanical stability point of view beyond structural stability of revision total knee arthoplasty itself.

• 한국지진공학회논문집
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• 제18권4호
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• pp.161-170
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• 2014

Newmark-type deformation analysis has rarely been done in Korea due to the popularity of simple pseudo-static limit equilibrium analysis and detailed time-history FE/FD dynamic analysis. However, the Korean seismic dam design code updated in 2011 prescribes Newmark-type deformation analysis as a major dynamic analysis method for the seismic evaluation of fill dams. In addition, a design PGA for dynamic analysis is significantly increased in the code. This paper aims to study the seismic evaluation of four existing large fill dams through advanced FEM/Newmark-type deformation analyses for the artificial earthquake time histories with the design PGA of 0.22g. Dynamic soil properties obtained from in-situ geo-physical surveys are applied as input parameters. For the FEM/Newmark analyses, sensitivity analyses are performed to study the effects of input PGA and $G_{max}$ of shell zone on the Newmark deformation. As a result, in terms of deformation, four fill dams are proved to be reasonably safe under the PGA of 0.22g with yield coefficients of 0.136 to 0.187, which are highly resistant for extreme events. Sensitivity analysis as a function of PGA shows that $PGA_{30cm}$ (a limiting PGA to cause the 30 cm of Newmark permanent displacement on the critical slip surface) is a good indicator for seismic safety check. CFRD shows a higher seismic resistance than ECRD. Another sensitivity analysis shows that $G_{max}$ per depth does not significantly affect the site response characteristics, however lower $G_{max}$ profile causes larger Newmark deformation. Through this study, it is proved that the amplification of ground motion within the sliding mass and the location of critical slip surface are the dominant factors governing permanent displacements.

• 대한토목학회논문집
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• 제34권6호
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• pp.1797-1806
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• 2014

지금까지 세계적으로 지하수 관리는 연간 지하수 함양량을 기반으로 한 안전채수량 정책에 의해 수행되어 왔다. 그러나 지하수 함양량은 시공간적으로 변동하므로 지속가능한 지하수자원을 평가하기 위해서는 동적 해석이 필요하다. 본 연구에서는 지표수-지하수 통합해석 모형인 SWAT-MODFLOW를 이용하여 경주지역의 지하수 함양량 공간분포를 산정하였다. 우리나라에서는 10년 빈도 갈수시 강수량에 함양계수를 곱하여 지역별 개발가능량을 산정하기에 본 연구에서는 기존 개발가능량을 평가하기 위해 빈도해석기법을 이용하였다. 소유역별 10년 빈도 갈수시의 지하수 함양량을 추정하는 보수적 방법을 제안하고 이를 기존 개발가능량과 비교하였다. 이같은 계산 절차를 통해 지하수 개발가능량을 산정하는 기존 절차의 한계를 합리적으로 제시할 수 있었다.

• 한국정보전자통신기술학회논문지
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• 제8권6호
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• pp.528-533
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• 2015

USN 환경의 구축에 있어서 무선통신을 이용한 안전한 센서 네트워크 구현은 전체 시스템에 있어서 가장 중요한 요소라고 할 수 있다. USN 통신은 접근성, 비접촉성을 높이기 위해 무선통신을 사용하고 있으나 이것으로 인한 보안의 취약성은 시스템을 위험하게 하고 있다. 우리는 이런 다중센서 네트워크인 USN에 효율적으로 적용할 수 있는 보안 프로토콜을 제안한다. 우리가 제안한 프로토콜은 개별 혼돈 시스템을 이용한 방법으로 각각의 센서에 장착된 혼돈시스템을 주 혼돈 시스템과 미리 준비된 킷값을 초깃값으로 동기화시키고, 동기화된 값을 대칭키로 하여 통신하는 보안 프로토콜이다. 본 논문에서 보인 통신 프로토콜은 USN의 취약한 보안 문제와 센서노드의 프로그램 용량 제한 문제점을 해결하기 위한 새로운 방법으로 계속적인 후속 연구가 이루어진다면 좋은 성과를 얻을 수 있을 것으로 생각된다.

• Structural Engineering and Mechanics
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• 제54권3호
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• pp.501-520
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• 2015

Presence of torsional loadings can significantly affect the flow of internal forces and deformation capacity of reinforced concrete (RC) columns. It increases the possibility of brittle shear failure leading to catastrophic collapse of structural members. This necessitates accurate prediction of the torsional behaviour of RC members for their safe design. However, a review of previously published studies indicates that the torsional behaviour of RC members has not been studied in as much depth as the behaviour under flexure and shear in spite of its frequent occurrence in bridge columns. Very few analytical models are available to predict the response of RC members under torsional loads. Softened truss model (STM) developed in the University of Houston is one of them, which is widely used for this purpose. The present study shows that STM prediction is not sufficiently accurate particularly in the post cracking region when compared to test results. An improved analytical model for RC square columns subjected to torsion with and without axial compression is developed. Since concrete is weak in tension, its contribution to torsional capacity of RC members was neglected in the original STM. The present investigation revealed that, disregard to tensile strength of concrete is the main reason behind the discrepancies in the STM predictions. The existing STM is extended in this paper to include the effect of tension stiffening for better prediction of behaviour of square RC columns under torsion. Three different tension stiffening models comprising a linear, a quadratic and an exponential relationship have been considered in this study. The predictions of these models are validated through comparison with test data on local and global behaviour. It was observed that tension stiffening has significant influence on torsional behaviour of square RC members. The exponential and parabolic tension stiffening models were found to yield the most accurate predictions.

• Clinical and Experimental Reproductive Medicine
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• 제15권2호
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• pp.149-155
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• 1988

To increase fertilization rate in vitro, separation of viable spermatozoa from the seminal plasma and its other components may be a useful procedure. Ejaculates from healthy men, whose semen analysis findings were normal in 19, and abnormal in 10, were filtered using the glass-wool filtration technique to yield a concentrated, viable sperm samples for IVF, and the usefulness and safety of this method were evaluated. The recovery rate of motile sperm in abnormal groups was 46.2% and 54.5% in normal group. The % motility was increased significantly compared with original sample after filtration, and the grade motility was improved, too. The sperm population with normal morphology was also increased significantly in both group. Using transmission electron microscopy, the ultrastructural integrity of acrosomal segment was examined in order to evaluate the potentially hazardous effect of glass-wool filtration to sperm head, however, sperm population with normal ultrastructure was increased compared with that of original ejaculate after separation. The filtered sperm was then processed for IVF, as the fertilizing capacity is the ultimate parameter of the sperm function. In abnormal group, the fertilization rate(41.5 %) and the ET rate per stimulated cycle were much lower than that of mormal group(69.6%). However, the cleavage rate and the number of embryos transfered per ET cycle were comparable with those of nomal group. The results suggest that the glass-wool filtration of sperm, particularly in oligo-asthenozoospsrmia, may be useful and safe method in the preparation of sperm for IVF.

• 대한약침학회지
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• 제14권3호
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• pp.19-45
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• 2011

Objectives: This study was performed to analyse the effects of Sweet Bee Venom(Sweet BV-pure melittin, the major component of honey bee venom) on the central nervous system in rats. Methods: All experiments were conducted at Biotoxtech Company, a non-clinical studies authorized institution, under the regulations of Good Laboratory Practice (GLP). Male rats of 5 weeks old were chosen for this study and after confirming condition of rats was stable, Sweet BV was administered in thigh muscle of rats. And checked the effects of Sweet BV on the central nervous system using the functional observational battery (FOB), which is a neuro-toxicity screening assay composed of 30 descriptive, scalar, binary, and continuous endpoints. And home cage observations, home cage removal and handling, open field activity, sensorimotor reflex test/physiological measurements were conducted. Results: 1. In the home cage observation, there was not observed any abnormal signs in rats. 2. In the observation of open field activity, the reduction of number of unit areas crossed and rearing count was observed caused by Sweet BV treatment. 3. In the observation of handling reactivity, there was not observed any abnormal signs in rats. 4. In the observation of sensorimotor reflex tests/physiological measurements, there was not observed any neurotoxic signs in rats. 5. In the measurement of rectal temperature, treatment of Sweet BV did not showed great influences in the body temperature of rats. Conclusions: Above findings suggest that Sweet BV is relatively safe treatment in the central nervous system. But in the using of over dose, Sweet BV may the cause of local pain and disturbance of movement. Further studies on the subject should be conducted to yield more concrete evidences.

• 대한약침학회지
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• 제18권2호
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• pp.67-75
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• 2015

Objectives: This study was carried out to analyze the single-dose toxicity of ShinYangHur (SYH) herbal acupuncture injected into the muscles of Sprague-Dawley (SD) rats. Methods: The SYH herbal acupuncture was made in a clean room at the Korean Pharmacopuncture Institute (KPI, Korea-Good Manufacturing Practice, K-GMP). After the mixing process with sterile distilled water, the pH was controlled to between 7.0 and 7.5. Then, NaCl was added to make a 0.9% isotonic solution by using sterilized equipment. All experiments were conducted at Biotoxtech, an institution authorized to perform non clinical studies under the regulations of Good Laboratory Practice (GLP). SD rats were chosen for the pilot study. Doses of SYH herbal acupuncture, 0.25, 0.5, and 1.0 mL, were administered to the experimental groups, and a dose of normal saline solution, 1.0 mL, was administered to the control group. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: No deaths or abnormalities occurred in any of the four groups. No significant changes in weight, hematological parameters or clinical chemistry between the control group and the experimental groups were observed. To check for abnormalities in organs and tissues, we used microscopy was used to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs or tissues. Conclusion: The above outcomes suggest that treatment with SYH herbal acupuncture is relatively safe. Further studies on this subject are needed to yield more concrete evidence.

• 대한약침학회지
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• 제14권1호
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• pp.5-24
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• 2011

Objective: This study was performed to analyse four weeks repeated -dose toxicity of Sweet Bee Venom (SBV-pure melittin, the major component of honey bee venom) in rats. Methods: All experiments were conducted under the regulations of Good Laboratory Practice (GLP) at Biotoxtech Company, a non-clinical study authorized institution. Male and female rats of 5 weeks old were chosen for the pilot study of four weeks repeated-dose toxicity and was injected at the level of 0.56 mg/kg body weight (eighty times higher than the clinical application dosage as the high dosage), followed by 0.28 and 0.14 mg/kg as midium and low dosage, respectively. Equal amount of normal saline was injected as the control group every day for four weeks. Results: 1. No mortality was witnessed in all of the experiment groups. 2. All experiment groups appealed pain sense in the treating time compared to the control group, and side effects such as hyperemia and movement disorder were observed around the area of injection in all experiment groups, and the higher dosage in treatment, the higher occurrence in side effects. 3. Concerning weight measurement, neither male nor female groups showed significant changes compared to the control group. 4. Concerning to the CBC and biochemistry, all experiment groups didn't show any significant changes compared to the control group. 5. Concerning weight measurement of organs, experiment groups didn't show any significant changes compared to the control group. 6. To verify abnormalities of organs and tissues, those such as cerebellum, cerebrum, liver, lung, kidney, and spinal cords were removed and we conducted histologocal observation with H-E staining. Concerning the histologocal observation of liver tissues, some fatty changes were observed around portal vein in 0.56 mg/kg experiment group. But another organs were not detected in any abnormalities. 7. The proper high dosage of SBV for the thirteen weeks repeated test in rats may be 0.28 mg/kg in one time. Conclusion: Above findings suggest that SBV is relatively safe treatment medium. Further studies on the subject should be conducted to yield more concrete evidences.