• The Journal of Internal Korean Medicine
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• v.16 no.1
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• pp.104-129
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• 1995

Sabaeksan and Sabaeksangagaryureuk, a traditional prescription, has been used in Korea for many centuries as a treatment for chronic respiratory disease. The purpose of the present study was to determine the effect of Sabaeksan and Sabaeksangagaryureuk on acetylcholine-induced tracheal smooth muscle contraction in guinea pigs and norepinephrine-induced vascular smooth muscle contraction in pigs. Guinea pig (500g, female) were killed by CO2 exposure and a segment (8-10mm) of the thoracic trachea from each guinea pig and renal artery from each pig were cut into equal segments and mounted 'in pairs' in a tissue bath. Contractile force was measured with force displacement transducers under 0.5g loading tension. The dose of acetylcholine (Ach) and norepinephrine (NE) which evoked 50% of maximal response ($ED_{50}$) was obtained from cumulative dose response curves for acetylcholine (10-7-10-4M) and norepinephrine (10-7-10-4M). Contractions of tracheal smooth muscle evoked by Ach ($ED_{50}$) were inhibited significantly by Sabaeksan and Sabaeksangagaryureuk. Propranolol (10-7M) slightly but significantly attenuated the inhibitory effects of Sabaeksan and Sabaeksangagaryureuk. Indomethacin and methylene blue (10-7M) did not significantly alter the inhibitory effect of Sabaeksan and Sabaeksanga-garyureuk. Contractions of vascular smooth muscle evoked by NE (NE50) were inhibited significantly by Sabaeksan and Sabaeksangagaryureuk. Propranolol (10-7M) slightly but significantly attenuated the inhibitory effects of Sabaeksan and Sabaeksangagaryureuk. Indomethacin and methylene blue (10-7M) did not significantly alter the inhibitory effect of Sabaeksan and Sabaeksangagaryureuk. These results indicate that Sabaeksan and Sabaeksangagaryureuk can relax acetylcholine-induced contraction of guinea pig tracheal smooth muscle, and norepinephrine-induced contraction of pig vascular smooth muscle that this inhibition involves, in part, the relation of adrenergic receptor.

• The Journal of Internal Korean Medicine
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• v.23 no.1
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• pp.15-23
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• 2002

Objective : We aimed to identify the dose-dependent inhibitory effects of Sabaek-san(瀉白散) and Sabaeksan plus Sasam(Adenophorae Radix) 瀉白散加沙蔘) on the mRNA expressions of Interleukin(IL)-6, IL-8 and granulocyte macrophage colony stimulating factor(GM-CSF) involved in the asthma model. Materials and Methods : Through this study, BEAS-2B cell lines, human epithelial cells were used. These cells were stimulated by tumor necrosis factor(TNF)-${\alpha}$, IL-1${\beta}$ and histamine for artificial inflammatory expression. ${\beta}$-action messenger RNA(mRNA) was used for standards. After each 24hours of Sabaeksan and Sabaeksan plus Sasam treatment, total cellular RNAs were collected by treating RNA zol directly on living cells, Then the transcriptional activities of IL-6, 8 and GM-CSF were measured by RT-PCR with electrophoresis, Results : The mRNA expressions of IL-6 are significantly inhibited compared to those of controlled group at 40 and 100ug/ml of Sabaeksan extract and $100{\mu}g/ml$ of Sabaeksan plus Sasam extract (p<0.05). The mRNA expressions of IL-8 are significantly inhibited compared to that of controlled group at 2.40 and 100 ug/ml of Sabaeksan extract and $40.100{\mu}g/ml$ of Sabaeksan plus Sasam extract(p<0.05) THe mRNA expressions of GM-CSF are significantly inhibited compared to those of the controlled group at $100{\mu}g/ml$ of Sabaeksan extract adn $40.100{\mu}g/ml$ of Sabaeksan plus Sasam extract.(p<0.05) Conclusions : This study shows that Sabaeksan and Sabaeksan plus Sasam have dose-dependent inhibitory effects on the mRNA expressions of IL-6, IL-8 and GM-CSF in human epithelial cells. Therefore, these types of herb medicine may inhibit the inflammatory process of asthma. Advanced studies are required to investigate the mechanisms of inhibition by herb medicine in the asthma model.

• The Journal of Internal Korean Medicine
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• v.12 no.1
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• pp.183-188
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• 1991

The following results were obtained according to these fact which is considered peviodically and bibliographically ; 1. The cause of bleeding of cough was Oegampunghan(外感風寒), Pyosaoesok(表邪外束), Chohwasangpye(燥火傷肺) in external cause, Eumhuhwadong(陰虛火動), Sinhu(腎虛), Hyulhu(血虛), Damhwajukyul(痰火積熱), Pyesuyul(肺受熱) ect. in internal cause. 2. The symptoms of bleeding of cough because of external cause was similar to generally cold symptom, because of internal cause were similar to Yeumhuhwadong(陰虛火動) and generanl Huyul(虛熱) symptom. 3. The treatment of external were Mahwangtang, Soshihotang, Sabaeksan ect. internal were Samultang, Yookmijihwangtang, Jaeumganghwatang, Gesosan ect.

• The Journal of Pediatrics of Korean Medicine
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• v.17 no.1
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• pp.53-69
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• 2003

This study was designed to investigate prescriptions and herbs for treating coughing In childhood(小兒咳嗽) with oriental medical literature. The results were summarized as follows; 1. 182 prescriptions and 212 herbs were used for healing coughing in childhood. 2. There are 12 cases which has the same name of prescription with different elements. Applied prescriptions can be recorded according to the number as follows. They are Samsoeum(蔘蘇飮) and Jeonglyukhwan, Ageosan(阿膠散), Gamgiltang(甘桔湯), Keumbichosan(金沸草散), Sabaeksan(瀉白散), Gilgyungtang(桔梗湯), Mahwangtang(麻黃湯), Baekbyungja(白餠子), Baeksanghwan(白祥丸). Boeunhwan, Whagaesan(華盖散) respectively. 3. Applied herbs can be recorded according to the number as follows. They are (杏仁), Radix glycyrrhizae(甘草), Rhizoma zingiberis(生薑), Rhizoma pinelliae(半夏), Radix ginseng(人蔘) Platycodi radix(桔梗), Mori cortex(桑白皮).