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Identification of quantitative trait loci for physical and chemical properties of rice grain

  • Cho, Yong-Gu;Kang, Hyeon-Jung;Lee, Young-Tae;Jong, Seung-Keun;Eun, Moo-Young;McCouch, Susan R.
    • Plant Biotechnology Reports
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    • v.4 no.1
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    • pp.61-73
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    • 2010
  • Quantitative trait loci (QTL) associated with six physical traits of cooked rice and seven chemical properties of rice grain were identified using a recombinant inbred (RI) population of rice evaluated over 3 years at the National Honam Agricultural Research Institute in Korea. The RI population consisted of 164 lines derived from a cross between Milyang23 and Gihobyeo, and the genetic map consisted of 414 molecular markers. A total of 49 QTL were identified for the 13 physico-chemical properties using composite interval mapping. Of these, 13 QTL were identified for 2 or more years, while 36 were detected in only 1 year. Five QTL were identified over all 3 years and will be useful for marker-assisted improvement of rice grain quality in Korea. The two QTL with the highest LOD scores, adhesiveness1.2 and potassium content7.1, provide a valuable starting point for positional cloning of genes underlying these QTL.

"Noise Perimeter Zones" - A New Systematic Method for Noise Exposure Reduction in Highway Construction Sites ("소음주의구역 (Noise Perimeter Zones)" - 고속도로 신설 공사 건설 현장에서의 소음 노출 저감을 위한 새로운 체계적인 방법)

  • An, Kwangseog;Moir, Susan
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.16 no.2
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    • pp.123-130
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    • 2006
  • 이 연구에서는 고속도로 신설 공사 건설 현장 근로자들의 소음 노출을 저감할 수 있는 방법들을 제시하며 특히 현장에서 적용할 수 있는 새로운 체계적인 방법인 "소음 주의 구역"을 제안한다. 고속도로 건설 현장에서 소음원이 되는 장비, 기계, 도구와 근로자들의 소음 노출 특성을 알아 보았으며, 개인청력 보호구, 공학적제어수단 (engineering controls), 관리적제어수단 (administrative controls)을 어떻게 적용할 것인가를 제시하였다. 개인청력 보호구만으로는 소음 노출을 효율적으로 저감시킬 수 없음을 보였으며, 공학적제어수단은 기술적 및 경제적 문제로, 관리적제어수단은 공사 기간 단축과 같은 실제적인 이유로 해서 시행 되기가 어렵다. 따라서 이 연구에서는 실제적으로 적용할 수 있는 체계적인 방법으로서 "소음주의구역 (Noise Perimeter Zones)"을 새롭게 제안하였다. 이 "소음주의구역"은 높은 수준의 소음원이 존재하는 고속도로 건설 공사 현장에 지정된다. 이 구역안에서는 근로자들의 출입이 통제되며 또한 개인청력 보호구, 공학적제어수단, 관리적제어수단과 같은 적절한 제어수단이 사용되어야 한다.

Positional Cloning and Phenotypic Characterization of a New Mutant Mouse with Neuronal Migration Abnormality

  • Park, Chankyu;Ackerman, Susan-L
    • Proceedings of the KSAR Conference
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    • 2001.10a
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    • pp.14-17
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    • 2001
  • Positional cloning (map-based cloning) of mutations or genetic variations has been served as an invaluable tool to understand in-vivo functions of genes and to identify molecular components underlying phenotypes of interest. Mice homozygous for the cerebellar deficient folia (cdf) mutation are ataxic, with cerebellar hypoplasia and abnormal lobulation of the cerebellum. In the cdf mutant cerebellum approximately 40% of Purkinje cells are ectopically located within the white matter and the inner granule cell layer (IGL). To identify the cdf gene, a high-resolution genetic map for the cdf-gene-encompassing region was constructed using 1997 F2 mice generated from C3H/HeSnJ-cdf/cdf and CAST/Ei intercross. The cdf gene showed complete linkage disequilibrium with three tightly linked markers D6Mit208, D6Mit359, and D6Mit225. A contig using YAC, BAC, and P1 clones was constructed for the cdf critical region to identify the gene. A deletion in the cdf critical region on chromosome 6 that removes approximately 150kb of DNA was identified. A gene associated with this deletion was identified using cDNA selection. cdf mutant mice with the transgenic copy of the identified gene restored the brain abnormalities of the mutant mice. The positional cloning of cdf gene provides a good example showing the identification of a gene could lead to finding a new component of important molecular pathways.

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Evaluation of the Performance on WindPRO Prediction in the Northeast Region of Jeju Island (제주 북동부지역을 대상으로 한 WindPRO의 예측성능 평가)

  • Oh, Hyun-Seok;Ko, Kyung-Nam;Huh, Jong-Chul
    • Journal of the Korean Solar Energy Society
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    • v.29 no.2
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    • pp.22-30
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    • 2009
  • In order to clarify predictive accuracy for the wind resource predicted by running WindPRO(Ver. 2.5) which is software for wind farm design developed by EMD from Denmark, an investigation was carried out at the northeast region of Jeju island. The Hangwon, Susan and Hoichun sites of Jeju island were selected for this study. The measurement period of wind at the sites was for one year. As a result, when the sites had different energy roses, though the two Wind Statistics made by STATGEN module were used for the prediction, it was difficult to exactly predict the energy rose at a given site. On the other hand, when the two Wind Statistics were used to predict the average wind speed, the wind power density and the annual energy production, the relative error was under ${\pm}20%$ which improved more than that when using only one Wind Statistics.

FUNCTIONAL PROPERTIES OF NATURAL CALCIUM FROM SEAWEEDS

  • Lawlor Susan
    • Proceedings of the Korean Society of Food Science and Nutrition Conference
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    • 2001.12a
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    • pp.126-133
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    • 2001
  • As dietary intakes levels of Calcium decrease below those recommended by health authorities the incidence of Osteoporosis is set to continue to rise. To address this issue it is becoming necessary to fortify regularly consumed foods such as cereals, biscuits etc. with ingredients such as AquaCal. AquaCal is a natural, organic source of calcium that is produced from calcified seaweed, Phymatolithon and Lithothamnion and can be utilised for Calcium and magnesium supplementation in a broad range of foods and beverages. AquaCal presents a great potential over other calcium sources because of its porous structure and it composition associating key minerals as calcium, magnesium and boron in addition to being neutral in taste in applications. Once consumed the efficacy of AquaCal must then be verified therefore a bioavailability a comparative feeding study to assess effects of AquaCal on bone density was carried out. There was no significant difference in bioavailability based on calcium absorption between AquaCal and Limestone however the weights of the femurs of the animals fed AquaCal did significantly increase over the group fed Limestone. As a natural and organic mineral AquaCal has many different fields of application.

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Unsteady Simulations of the Flow in a Swirl Generator, Using OpenFOAM

  • Petit, Olivier;Bosioc, Alin I.;Nilsson, Hakan;Muntean, Sebastian;Susan-Resiga, Romeo F.
    • International Journal of Fluid Machinery and Systems
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    • v.4 no.1
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    • pp.199-208
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    • 2011
  • This work presents numerical results, using OpenFOAM, of the flow in the swirl flow generator test rig developed at Politehnica University of Timisoara, Romania. The work shows results computed by solving the unsteady Reynolds Averaged Navier Stokes equations. The unsteady method couples the rotating and stationary parts using a sliding grid interface based on a GGI formulation. Turbulence is modeled using the standard k-${\varepsilon}$ model, and block structured wall function ICEM-Hexa meshes are used. The numerical results are validated against experimental LDV results, and against design velocity profiles. The investigation shows that OpenFOAM gives results that are comparable to the experimental and design profiles. The unsteady pressure fluctuations at four different positions in the draft tube is recorded. A Fourier analysis of the numerical results is compared whit that of the experimental values. The amplitude and frequency predicted by the numerical simulation are comparable to those given by the experimental results, though slightly over estimated.

Effects of Age and High Frequency Hearing Loss on Binaural Speech Understanding Using HINT Study (HINT 검사를 이용한 양이의 어음이해에 있어서 노화와 고주파수 청력의 효과)

  • Kim, Sung-Hee;Frisina Robert D.;Frisina Susan T.;Mapes Frances M.;Hickman Elizabeth D.;Frisina D. Robert
    • The Journal of the Acoustical Society of Korea
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    • v.26 no.1E
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    • pp.8-13
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    • 2007
  • It has long been known that high-frequency sensorineural hearing loss (HFHL) can contribute to difficulty in speech understanding by elderly listeners. This study evaluated the relative contribution of HFHL and age to speech understanding. Subjects included adult middle-aged and old groups with normal hearing or with limited HFHL. The Hearing-in-Noise-Test (HINT) was used to measure speech perception performance in quiet and in noise. The middle-aged groups showed significant effects of HFHL for speech intelligibility in quiet and in noise, but the old groups showed the difference in quiet only due to high frequency hearing. The results suggest that HFHL may affect speech intelligibility differently with age and therefore hearing aid selection needs to take into account the influence of age.

Effects of Tea Constituents on Intracellular Level of the Major Tea Catechin, (-)-Epigallocatechin-3-gallate

  • Hong, Jun-Gil;Yang, Chung-S.
    • Food Science and Biotechnology
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    • v.16 no.1
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    • pp.167-170
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    • 2007
  • (-)-Epigallocatechin-3-gallate (EGCG), a mai or tea catechin has been shown to have many interesting biological activities. In the present study, we studied the effects of green tea catechins, EGCG metabolites, and black tea theaflavins on accumulation of EGCG in HT-29 human colon cells. Intracellular levels of [$^3H$]-EGCG were not changed significantly in the presence of other tea catechins including (-)-epicatechin, (-)-epigallocatechin, and (-)-epicatechin-3-gallate. EGCG methyl metabolites and EGCG 4"-glucuronide did not affect cellular levels of [$^3H$]-EGCG. Black tea theaflavins and theasinensin A (TsA), an EGCG oxidative dimer, however, significantly decreased cellular accumulation of EGCG in HT-29 cells by 31-56%. This decrease was more pronounced when cells were incubated in the presence of theaflavin-3',3"-digallate (TFdiG) or TsA. When EGCG was added separately from TFdiG or TsA, the accumulation of EGCG in HT-29 cells was also significantly decreased regardless of when TFdiG or TsA was added during the uptake study (p<0.01). The results suggest that theaflavins and TsA may interrupt EGCG absorption through the gastrointestinal epithelium.

A Study on Health Behaviors, Health Status and Anxiety about Aging for the Elderly - Focused on the Elderly in Senior Center- (경로당 이용노인의 노화에 대한 불안감, 지각된 건강상태와 건강증진 행위실천)

  • Shin, Yoo-Sun;Kim, Eun-Ha
    • Research in Community and Public Health Nursing
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    • v.20 no.2
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    • pp.179-188
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    • 2009
  • This study is conducted to survey and examine the relationships among anxiety about aging, perceived health status and health promoting behaviors in the elderly, and to provide basic data for health promoting interventions that would improve their successful aging. Me1hods: Data in this study was collected from 333 elderly participants living in Susan. Descriptive statistics, t-test or ANOVA with Scheffe's test, Pearson's correlation coefficients and stepwise multiple regression were used for data analysis. Results: The major findings of this study were as follows. 1) The mean score of anxiety about aging was 2.67 $\pm$ 0.30, perceived health status 2.46 $\pm$ 0.37, and health promoting behavior 2.77 $\pm$ 0.21.2) There was a positive correlation between health promoting behavior and perceived health status (r = 267, P= .000). There was a negative correlation between anxiety about aging and health promoting behavior (r = -.163, P=.003). 3) Health-promoting behavior was significantly associated with perceived health and anxiety about aging, which explained 20.9% of variance in health-promoting behavior. Conclusion: In order to promote perceived health status and to decrease anxiety about aging in the elderly, it is necessary to develop supporting interventions to decrease anxiety about aging

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Comparison of Two Methods for Measuring Daily Path Lengths in Arboreal Primates

  • Lappan, Susan
    • Journal of Ecology and Environment
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    • v.30 no.2
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    • pp.201-207
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    • 2007
  • Researchers have used a variety of methods to measure patterns of animal movement, including the use of spatial data (mapping the position of a moving animal at specified intervals) and direct estimation of travel path length by pacing under a moving animal or group. I collected movement data from five groups of siamangs (Symphalangus syndactylus) using two different methods concurrently to estimate the effects of the method of data collection on estimates of daily path length (DPL). Estimates of DPL produced from spatial data collected at 15-minute intervals were 12% lower than estimates of DPL produced by pacing under the traveling animal. The actual magnitude of the difference was correlated with the travel distance, but there was no correlation between the proportional difference and the travel distance. While the collection of spatial data is generally preferable, as spatial data permit additional analyses of patterns of movements in two or three dimensions, the relatively small difference between the DPL's produced using different methods suggests that pacing is an acceptable substitute where the collection of spatial data is impractical. I also subsampled the spatial data at increasing time intervals to assess the effect of sampling interval on the calculation of daily path lengths. Longer sampling intervals produced significantly shorter estimates of travel paths than shorter sampling intervals. These results suggest that spatial data should be collected at short time intervals wherever possible, and that sampling intervals should not exceed 30 minutes. Researchers should be cautious when comparing data generated using different methods.