• Molecules and Cells
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• v.42 no.9
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• pp.672-685
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• 2019

Currently, liver transplantation is the only available remedy for patients with end-stage liver disease. Conservation of transplanted liver graft is the most important issue as it directly related to patient survival. Carbonyl reductase 1 (CBR1) protects cells against oxidative stress and cell death by inactivating cellular membrane-derived lipid aldehydes. Ischemia-reperfusion (I/R) injury during living-donor liver transplantation is known to form reactive oxygen species. Thus, the objective of this study was to investigate whether CBR1 transcription might be increased during liver I/R injury and whether such increase might protect liver against I/R injury. Our results revealed that transcription factor Nrf2 could induce CBR1 transcription in liver of mice during I/R. Pre-treatment with sulforaphane, an activator of Nrf2, increased CBR1 expression, decreased liver enzymes such as aspartate aminotransferase and alanine transaminase, and reduced I/R-related pathological changes. Using oxygen-glucose deprivation and recovery model of human normal liver cell line, it was found that oxidative stress markers and lipid peroxidation products were significantly lowered in cells overexpressing CBR1. Conversely, CBR1 knockdown cells expressed elevated levels of oxidative stress proteins compared to the parental cell line. We also observed that Nrf2 and CBR1 were overexpressed during liver transplantation in clinical samples. These results suggest that CBR1 expression during liver I/R injury is regulated by transcription factor Nrf2. In addition, CBR1 can reduce free radicals and prevent lipid peroxidation. Taken together, CBR1 induction might be a therapeutic strategy for relieving liver I/R injury during liver transplantation.

• Korean Journal of Ecology and Environment
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• v.51 no.4
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• pp.345-353
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• 2018

Hibernation is regarded as a physiological and behavioral adaptation that permits the survival of animals such as bats during seasonal periods of energy shortage. This study investigated the hibernation period of Plecotus ognevi in the temperate climate zone and the relationship between the thermal preference and hibernating process of bats. We hypothesized that the hibernation period of bats is closely related to the external temperature and temperature preference of bat species in the temperate region. To verify this hypothesis, we surveyed the distribution of the P. ognevi population in South Korea, and the temperature preference and the characteristics of hibernacula of P. ognevi. We predict that hibernation in the bat will begin when the external temperature drops below the thermal preference of the species and will leave from hibernation when the external temperature is higher than the thermal preference. P. ognevi hibernated in roosts maintained in low temperature ambient conditions with $-3.5{\sim}7.5^{\circ}C$). The body temperatures (averaged $3.01{\pm}1.30^{\circ}C$, ranged $0.1{\sim}7.8^{\circ}C$) of hibernating bats were closely related to the rock surface temperatures rather than the ambient temperatures. The bats began to hibernate in late November and final arousals occurred in mid-March, so that the total length of the hibernation was 115~120 days. The period of hibernation was strongly influenced by fluctuations in the external mean temperature. This study suggests that the onset and termination of P. ognevi hibernation is due to the interaction between the temperature of the hibernacula and that of the external environment and is based on the thermal preference of the bats. The study also suggests that the hibernation strategy such as thermal preference and hibernation periods of this species affect to distribution as bat species adapting to a severely climate.

• Journal of Internet of Things and Convergence
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• v.8 no.6
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• pp.115-122
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• 2022

Recently, the importance of CPR training for the layperson has been emphasized to improve the survival rate of out-of-hospital cardiac arrest patients. An accurate and realistic training strategy is required for the CPR training effect for laypersons. In this study, we develop an extended reality (XR) based CPR training system and evaluate its usability. The XR based CPR training system consisted of three applications. First, a 3D heart anatomy image registered to the manikin is transmitted to the smart glasses to guide the chest compression point. The second application provides visual and auditory information about the CPR process through smart glasses. At the same time, the smartwatch sends a vibration notification to guide the compression rate. The 'Add-on-kit' is a device that detects the depth and speed of chest compression via sensors installed on the manikin and sends immediate feedback to the smartphone. One hundred laypersons who participated in this study agreed that the XR based CPR training system has realism and effectiveness. XR based registration technology will contribute to improving the efficiency of CPR training by enhancing realism, immersion, and self-directed learning.

• Korean journal of applied entomology
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• v.61 no.1
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• pp.91-100
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• 2022

Ticks are the arthropod vector capable of transmitting diverse pathogens, which include bacteria, viruses, protozoan and fungi. Ticks are able to survive under stressful environmental conditions. One of evolutionary outcomes of these obligatory hematophagous arthropods is the survival for extended periods of time without a blood meal during off-host periods. Water conservation biology and heat tolerance have allowed ticks to thrive even under high temperatures and low relative humidity, thus they have become highly successful arthropods as they are distributed globally. Tick osmoregulatory physiology is a complex mechanism, which involves multiple osmoregulatory organs (salivary glands, Malpighian tubules, hindgut and synganglion) for the acquisition and excretion of water and ions. Blood feeding and water vapor uptake have been early reported as the primary passages for ixodid tick to acquire water. Recently, we have learned that ticks can actively drink environmental water allowing hydration. The acquired water can be traced to the salivary glands (type I acini) and the midgut diverticula. This opens new avenues for tick management through the delivery of toxic agents into their drinking water, in addition to an alternative strategy for the study of tick physiology. Here we address the osmoregulatory physiology in the ixodid ticks as a potential target physiological mechanism for tick control. We discuss the implications of water drinking behavior for tick control through the delivery of toxic agents and discuss the dermal excretion physiology as an additional pathway to induce tick dehydration and tick death.

• Journal of the Korea Convergence Society
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• v.13 no.5
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• pp.327-332
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• 2022

In the age of population cliff, an aggressive entrance exam strategy for the development of excellent human resources for beauty is very important for the survival of beauty departments and universities. Therefore, for the purpose of in-depth trend analysis of department names and affiliated departments that reflect department characteristics as well as external department promotion tools, we investigated and analyzed department affiliated departments and department titles in 2020 targeting the Beauty Department of Central University. As a result of the study, the departments belonging to each of the four regions had the most beauty arts department (28.57%) and health department (50.00%). In Daejeon, beauty design (37.5%) was the most, and in Chungcheong, beauty care, beauty cosmetics, and medical beauty care (11.11%) were the most. Through this study, it will be possible to analyze the overall characterization trend of the Department of Beauty, and it will be used as an important basic data to suggest the future direction of the department when the departments are merged or separated in the future. In addition, I believe that it will provide a foothold for follow-up research on the curriculum for each department or the change of department name by year.

• Journal of Ginseng Research
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• v.47 no.2
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• pp.199-209
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• 2023

Background: Due to the interrupted blood supply in cerebral ischemic stroke (CIS), ischemic and hypoxia results in neuronal depolarization, insufficient NAD+, excessive levels of ROS, mitochondrial damages, and energy metabolism disorders, which triggers the ischemic cascades. Currently, improvement of mitochondrial functions and energy metabolism is as a vital therapeutic target and clinical strategy. Hence, it is greatly crucial to look for neuroprotective natural agents with mitochondria protection actions and explore the mediated targets for treating CIS. In the previous study, notoginseng leaf triterpenes (PNGL) from Panax notoginseng stems and leaves was demonstrated to have neuroprotective effects against cerebral ischemia/reperfusion injury. However, the potential mechanisms have been not completely elaborate. Methods: The model of middle cerebral artery occlusion and reperfusion (MCAO/R) was adopted to verify the neuroprotective effects and potential pharmacology mechanisms of PNGL in vivo. Antioxidant markers were evaluated by kit detection. Mitochondrial function was evaluated by ATP content measurement, ATPase, NAD and NADH kits. And the transmission electron microscopy (TEM) and pathological staining (H&E and Nissl) were used to detect cerebral morphological changes and mitochondrial structural damages. Western blotting, ELISA and immunofluorescence assay were utilized to explore the mitochondrial protection effects and its related mechanisms in vivo. Results: In vivo, treatment with PNGL markedly reduced excessive oxidative stress, inhibited mitochondrial injury, alleviated energy metabolism dysfunction, decreased neuronal loss and apoptosis, and thus notedly raised neuronal survival under ischemia and hypoxia. Meanwhile, PNGL significantly increased the expression of nicotinamide phosphoribosyltransferase (NAMPT) in the ischemic regions, and regulated its related downstream SIRT1/2/3-MnSOD/PGC-1α pathways. Conclusion: The study finds that the mitochondrial protective effects of PNGL are associated with the NAMPT-SIRT1/2/3-MnSOD/PGC-1α signal pathways. PNGL, as a novel candidate drug, has great application prospects for preventing and treating ischemic stroke.

• Korean journal of applied entomology
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• v.61 no.4
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• pp.563-575
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• 2022

Maruca vitrata is one of important pests in leguminous crops, especially red bean. We investigated the effects of temperature on development of each life stage, adult longevity and fecundity of M. vitrata for understanding the biological characteristics of the insect species at eight constant temperatures of 13, 16, 19, 22, 25, 28, 31, and 34℃. Eggs hatched successfully at all temperature subjected and larvae successfully developed to the adult stage from 16℃ to 31℃. The developmental period of egg decreased up to 31℃ and after then increased. The developmental period of larva and pupa, and adult longevity of M. vitrata decreased with increasing temperature. Lower and higher threshold temperature (TL and TH) were calculated by the Lobry-Rosso-Flandrois (LRF) and Sharpe-Schoolfield-Ikemoto (SSI) models. The lower developmental threshold (LDT) and thermal constant (K) from egg hatching to adult emergence of M. vitrata were estimated by linear regression as 12.8℃ and 280.8DD, respectively. TL and TH from egg hatching to adult emergence using SSI model were 14.2℃ and 31.9℃. Thermal windows, i.e., the range in temperature between the minimum and maximum rate of development, of M. vitrata was 17.7℃. In addition, we constructed the oviposition models of adult, using the investigated adult traits including survival, longevity, oviposition period and fecundity. Temperature-dependent development models and adult oviposition models will be helpful to understand the population dynamics of M vitrata and to establish the strategy of integrated pest management in legume crops.

• Korean journal of applied entomology
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• v.61 no.4
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• pp.577-590
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• 2022

Ostrinia scapulalis is one of important pests in leguminous crops, especially red bean. In order to understand the biological characteristics of the insect, we investigated the effects of temperature on development of each life stage, adult longevity and fecundity of O. scapulalis at eleven constant temperatures of 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, and 36℃. Eggs and larvae successfully developed next life stage at most temperature subjected except 7, 10 and 13℃. The developmental period of egg, larva and pupa decreased as temperature increased. Lower and higher threshold temperature (TL and TH) were calculated by the Lobry-Rosso-Flandrois (LRF) and Sharpe-Schoolfield-Ikemoto (SSI) models. The lower developmental threshold (LDT) and thermal constant (K) from egg hatching to adult emergence of O. scapulalis were estimated by linear regression as 13.5℃ and 384.5DD, respectively. TL and TH from egg hatching to adult emergence using SSI model were 19.4℃ and 39.8℃. Thermal windows, i.e., the range in temperature between the minimum and maximum rate of development, of O. scapulalis was 20.4℃. Adults produced viable eggs at the temperature range between 16℃ and 34℃, and showed a maximum number, ca. 416 offsprings, at 25℃. Adult models including aging rate, age-specific survival rate, age-specific cumulative oviposition, and temperature-dependent fecundity were constructed, using the temperature-dependent adult traits. Temperature-dependent development models and adult oviposition models will be useful components to understand the population dynamics of O. scapulalis and will be expected using a basic data for establishing the strategy of integrated pest management in leguminous crops.

• East European & Balkan Studies
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• v.32
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• pp.257-281
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• 2012

Are the policies and strategies which weak and small states choose to follow for ensuring their's own security meaningless? Can the fate of weak states be guaranteed only by the neighboring benign strong states and be in certain international relations favoring for them? In this paper, to answer these questions, what kinds of security policies and foreign policies had small states chosen which were in the face of threats of great powers will be examined. Between the two world war, the series of event which took place in central and eastern European countries(CEEc) have been selected to examine the hypothesis and theories of international relations and foreign policies, and at the same time cited as examples of failed foreign policy and security strategy. During interwar periods, CEEc were the prime examples for examining what kind of policies and strategies had the small states chosen over the international relations in which the smalls had to struggle to survive between neighbouring the powers and between the hostiles. Furthermore, for the measures which the smalls had chosen had no effect when the great power threatened military to them, the main purpose of this paper is to examine what was the best choose to depend their survival. For this purpose, in this paper, several strategies which the smalls can choose and the counterfactuals, that is "if the smalls chosen another strategies, they could...", will be examined.

• Journal of agriculture & life science
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• v.50 no.1
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• pp.125-136
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• 2016

The Korea Forest Service(KFS) has been initiating restoration activities of ridgeline in damaged and fragmented areas of Baekdudaegan since 2011. Completed project in Ihwaryeong, Yuksimnyeong & Beoljae(2012; 2013) were selected as sites for this study. The changes in microclimate condition, vegetation composition and soil properties between project sites and adjacent stands were compared to evaluate the effect of restoration at early stage(after 2years). Pinus densiflora was planted mainly for these restoration sites, however Robinia pseudoacacia and Alnus sibirica invaded the area two years after the restoration activities. Ihwaryeong showed the most changes in understory vegetation among the study sites. Exotic species, such as Ambrosia artemisiifolia, Oenothera odorata, Erigeron annuus, and Coreopsis tinctoria invaded Ihwaryeong, and the dominance have invaded currently. It resulted from the poor survival rate of trees and high difference in microclimate wherein there's an increased temperature and decreased humidity in both restored sites and adjacent stands. In addition, it is also caused by poor soil chemical property, especially pH and organic matter content due to lack of humus layer and its accumulation, compared to adjacent forest soil in restored sites. Significant difference on chemical soil property was observed between restored sites and adjacent forest but no significant difference was observed after two years of restoration. Ecological monitoring is needed to understand the ecological changes after restoration and to establish a long-term management strategy.