• The Korean Journal of Physiology and Pharmacology
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• 제20권4호
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• pp.357-366
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• 2016

Pro-inflammatory cytokine and brain-derived neurotrophic factor (BDNF) are modulated in post-traumatic stress disorder (PTSD). This study investigated the effects of ibuprofen (IBU) on enhanced anxiety in a rat model of PTSD induced by a single prolonged stress (SPS) procedure. The effects of IBU on inflammation and BDNF modulation in the hippocampus and the mechanisms underlying for anxiolytic action of IBU were also investigated. Male Sprague-Dawley rats were given IBU (20 or 40 mg/kg, i.p., once daily) for 14 days. Daily IBU (40 mg/kg) administration significantly increased the number and duration of open arm visits in the elevated plus maze (EPM) test, reduced the anxiety index in the EPM test, and increased the time spent in the center of an open field after SPS. IBU administration significantly decreased the expression of pro-inflammatory mediators, such as tumor necrosis $factor-{\alpha}$, $interleukin-1{\beta}$, and BDNF, in the hippocampus, as assessed by reverse transcription-polymerase chain reaction analysis and immunohistochemistry. These findings suggest that IBU exerts a therapeutic effect on PTSD that might be at least partially mediated by alleviation of anxiety symptoms due to its anti-inflammatory activity and BDNF expression in the rat brain.

• Journal of Ginseng Research
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• 제45권5호
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• pp.575-582
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• 2021

Background: In ginseng, there exists a glycolipoprotein complex with a special form of lipid LPAs called Gintonin. The purpose of this study is to show that Gintonin has a therapeutic effect on rheumatoid arthritis through LPA2 receptors. Methods: Fibroblast-like synoviocytes (FLS) were treated with Gintonin and stimulated with interleukin (IL)-1β. The antioxidant effect of Gintonin was measured using MitoSOX and H₂DCFDA experiments. The anti-arthritic efficacy of Gintonin was examined by analyzing the expression levels of inflammatory mediators, phosphorylation of mitogen-activated protein kinase (MAPK) pathways, and translocation of nuclear factor kappa B (NF-κB)/p65 into the nucleus through western blot. Next, after treatment with LPAR2 antagonist, western blot analysis was performed to measure inflammatory mediator expression levels, and NF-κB signaling pathway. Carrageenan/kaolin-induced arthritis rat model was used. Rats were orally administered with Gintonin (25, 50, and 100 mg/kg) every day for 6 days. The knee joint thickness, squeaking score, and weight distribution ratio (WDR) were measured as the behavioral parameters. After sacrifice, H&E staining was performed for histological analysis. Results: Gintonin significantly inhibited the expression of iNOS, TNF-α, IL-6 and COX-2. Gintonin prevented NF-κB/p65 from moving into the nucleus through the JNK and ERK MAPK phosphorylation in FLS cells. However, pretreatment with an LPA2 antagonist significantly reversed these effects of Gintonin. In the arthritis rat model, Gintonin suppressed all parameters that were measured. Conclusion: This study suggests that LPA2 receptor plays a key role in mediating the anti-arthritic effects of Gintonin by modulating inflammatory mediators, the MAPK and NF-κB signaling pathways.

• Journal of Ginseng Research
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• 제45권4호
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• pp.510-518
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• 2021

Background: Gintonin is a newly derived glycolipoprotein from the roots of ginseng. The purpose of this study is to investigate the anti-arthritic efficacy of Gintonin on various proteases and inflammatory mediators that have an important role in arthritis. Methods: Fibroblast-like synoviocytes (FLS) were treated with Gintonin and stimulated with interleukin (IL)-1β 1 hour later. The antioxidant effect of Gintonin was measured using MitoSOX and H₂DCFDA experiments. The anti-arthritic efficacy of Gintonin was examined by analyzing the expression levels of inflammatory mediators using RT-PCR, western blot, and ELISA. The phosphorylation of mitogen-activated protein kinase (MAPK) pathways and translocation of nuclear factor kappa B (NF-κB)/p65 into the nucleus were also analyzed using western blot, ELISA, and immunocytochemistry. Collagen-induced arthritis (CIA) mice model was used. Mice were orally administered with Gintonin (25, 50, and 100 mg/kg) every 2 days for 45 days. The body weight, arthritis score, squeaking score, and paw volume were measured as the behavioral parameters. After sacrifice, H&E and safranin-O staining were performed for histological analysis. Results: Gintonin significantly inhibited the expression of inflammatory intermediates. Gintonin prevented NF-κB/p65 from moving into the nucleus through the JNK and ERK MAPK phosphorylation in FLS cells. Moreover, Gintonin suppressed the symptoms of arthritis in the CIA mice model. Conclusion: As a result, the antioxidant and anti-inflammatory effects of Gintonin were demonstrated, and ultimately the anti-arthritic effect was proved. Collectively, Gintonin has a great potential as a therapeutic agent for arthritis treatment.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2023년도 학술발표회
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• pp.43-43
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• 2023

최근 기후변화로 인해 강수량 및 강우패턴이 변화하고 있으며, 기록적인 가뭄이나 홍수와 같은 극한사상의 발생빈도가 점차 증가하고 있다. 국외의 경우 2000년부터 2021년까지 미국 서부 지역에서 극한 가뭄사상이 발생하였으며, 호주에서는 2017년부터 2019년까지 호주 남동부와 뉴사우스웨일스 지역에 극심한 가뭄이 나타났다. 국내의 경우 2000년대에 들어서 가뭄이 국지적으로 빈번하게 발생하고 있으며, 2022년부터 20023년까지 전라남도 지역에 극심한 가뭄이 발생하였다. CMIP6(Climate Model Intercomparison Project Phase 6)는 기후변화에 관한 정부간 협의체(Intergovernmental Panel on Climate Change, IPCC) 6차 평가보고서 (Sixth Assessment Report, AR6)에서 기후 모델 간의 비교와 평가를 위해 설립된 국제 협업 프로젝트로 기후변화를 예측하기 위해 다양한 기후 모델을 사용하여 미래의 기후 시나리오를 제시하였다. SSP (Shared Socioeconomic Pathways) 시나리오는 CMIP6에서 사용되는 미래 사회경제적 발전 경로를 나타내며, 기후변화의 다양한 미래 상황을 평가 및 기후영향을 분석할 수 있다. 국내 논 용수는 주로 저수지와 같은 수리시설물을 통해 공급되는 반면, 밭 용수의 경우 수리시설물로부터 용수를 공급받는 관개전은 일부에 불과하고 대부분의 밭의 경우 용수공급을 강우에 의존하여 가뭄에 더욱 취약한 실정이다. 본 연구에서는 CMIP5 기후모델 기반 RCP (Representative Concentration Pathways) 시나리오 및 CMIP6 기후모델 기반 SSP 시나리오를 적용하여 미래 기후 데이터를 비교하고자 한다. 또한, 미래 기후변화 시나리오를 토양수분모형을 적용하여 미래 밭가뭄을 전망하고자 한다.

• 한국조경학회지
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• 제52권2호
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• pp.96-109
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• 2024

본 연구는 딥러닝 모델 중 VGG-16 및 ResNet50 모델을 활용하여 전시 정원의 유사성 평가 방법을 제시하는 것에 목적이 있다. VGG-16과 ResNet50 모델을 기반으로 전시 정원 유사성 판단을 위한 모형을 개발하였고, 이를 DRG(deep recognition of similarity in show garden design)모형이라 한다. 평가를 위한 방법으로 GAP와 피어슨 상관계수를 활용한 알고리즘을 사용하여 모형을 구축하고 1순위(Top1), 3순위(Top3), 5순위(Top5)에서 원본 이미지와 유사한 이미지를 도출하는 총 개수 비교로 유사성의 정확도를 분석하였다. DRG 모형에 활용된 이미지 데이터는 국외 쇼몽가든페스티벌 전시 정원 총 278개 작품과 국내 정원박람회인 서울정원박람회 27개 작품 및 코리아가든쇼 전시정원 이미지 17개 작품이다. DRG모형을 활용하여 동일 집단과 타 집단간의 이미지 분석을 진행하였고, 이를 기반으로 전시 정원 유사성의 가이드라인을 제시하였다. 첫째, 전체 이미지 유사성 분석은 ResNet50 모델을 기반으로 하여 데이터 증강 기법을 적용하는 것이 유사성 도출에 적합하였다. 둘째, 내부 구조와 외곽형태에 중점을 둔 이미지 분석에서는 형태에 집중하기 위한 일정한 크기의 필터(16cm × 16cm)를 적용하여 이미지를 생성하고 VGG-16 모델을 적용하여 유사성을 비교하는 방법이 효과적임을 알 수 있었다. 이때, 이미지 크기는 448 × 448 픽셀이 효과적이며, 유채색의 원본 이미지를 기본으로 설정함을 제안하였다. 이러한 연구 결과를 토대로 전시 정원 유사성 판단에 대한 정량적 방법을 제안하고, 향후 다양한 분야와의 융합 연구를 통해 정원 문화의 지속적인 발전에 기여할 것으로 기대한다.

• 대한원격탐사학회지
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• 제28권4호
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• pp.435-448
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• 2012

증발산은 토양 표면에서 일어나는 증발 과정과 식물의 광합성 작용으로 인해 일어나는 증산 작용을 포함하는 수문기상인자로 외부 환경에 민감하게 작용한다. 현재 국내외에서는 이를 정확하게 관측하여 활용하기 위해 증발접시(evaporation pan), 침루계(lysimeter) 등을 이용하여 실측하거나 Eddy covariance technique, Bowen ratio method 등을 이용하여 경험적으로 산정하고 있으나 공간적인 제약이 따른다. 따라서 본 연구에서는 Terra 인공위성에 탑재된 Moderate Resolution Imaging Spectroradiometer (MODIS) 다중분광 센서를 이용, 원격탐사 기술을 적용함으로써 이러한 지상 관측의 단점을 보완하고자 하였다. 이전 연구들에서 소개가 되었던 원격탐사 기반 증발산 산정 모형을 개선하여 별도의 외부 입력자료 없이 MODIS 위성 이미지 자료만을 이용, 우리나라의 지역적 특성을 반영한 Penman-Monteith 기반 증발산을 산정하였다. 유량조사사업단에서 운영 및 관리하고 있는 설마천/청미천 플럭스 타워의 증발산 관측치와 MODIS 기반 증발산 산정값과의 비교를 통해 각각 0.69, 0.74의 높은 상관계수를 보여 산정 방법의 적용성을 검증하였다.

• 한국습지학회지
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• 제13권3호
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• pp.471-479
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• 2011

증발산은 토양 표면에서 일어나는 증발 과정과 식물의 광합성으로 인해 발생하는 증산 작용을 포함한 수문 기상인자로 수문 순환과정에서 중요한 역할을 차지한다. 현재 국내외에서는 증발산을 산정하고 공간적인 거동을 파악하기 위한 연구가 활발히 진행되고 있지만 특정 지역에서의 토지 피복의 차이나 식생으로 인해 거동을 이해하는데 많은 제약이 따른다. 본 연구에서는 고해상도의 영상을 제공하는 Landsat 위성이 기반이 되는 원격탐사 기반 에너지 수지 모형인 Mapping EvapoTRanspiration with Internalized Calibration (METRIC) 모형과 MODerate resolution Imaging Spectroradiometer (MODIS) 위성 기반의 Penman-Monteith 알고리즘으로 산정된 증발산의 공간 분포를 비교하였다. 토지 피복별로 분류한 후 두 공간 분포를 비교하여 침엽수림과 활엽수림에서 가장 높은 상관관계를 갖는 것을 확인하였고 두 모형에 대한 적용성이 높음을 알 수 있다. 본 연구를 바탕으로 원격탐사 기반 고해상도 증발산 지도를 제작하여 시공간적 변동성과 계절 변화에 따른 거동을 파악할 수 있을 것이다.

• Biomolecules & Therapeutics
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• 제21권5호
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• pp.381-390
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• 2013

The purpose of this study was to examine whether ginsenoside Rg3 (GRg3) could improve learning and memory impairments and inflammatory reactions induced by injecting lipopolysaccharide (LPS) into the brains of rats. The effects of GRg3 on proinflammatory mediators in the hippocampus and the underlying mechanisms of these effects were also investigated. Injection of LPS into the lateral ventricle caused chronic inflammation and produced deficits in learning in a memory-impairment animal model. Daily administration of GRg3 (10, 20, and 50 mg/kg, i.p.) for 21 consecutive days markedly improved the LPS-induced learning and memory disabilities demonstrated on the step-through passive avoidance test and Morris water maze test. GRg3 administration significantly decreased expression of pro-inflammatory mediators such as tumor necrosis factor-${\alpha}$, interleukin-1${\beta}$, and cyclooxygenase-2 in the hippocampus, as assessed by reverse transcription-polymerase chain reaction analysis and immunohistochemistry. Together, these findings suggest that GRg3 significantly attenuated LPS-induced cognitive impairment by inhibiting the expression of pro-inflammatory mediators in the rat brain. These results suggest that GRg3 may be effective for preventing or slowing the development of neurological disorders, including Alzheimer's disease, by improving cognitive and memory functions due to its anti-inflammatory activity in the brain.

• The Korean Journal of Physiology and Pharmacology
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• 제18권3호
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• pp.191-200
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• 2014

We investigated the anxiolytic-like activity of ${\alpha}$-asarone (AAS) from Acorus gramineus in an experimental rat model of anxiety induced by repeated administration of the exogenous stress hormone corticosterone (CORT). The putative anxiolytic effect of AAS was studied in behavioral tests of anxiety, such as the elevated plus maze (EPM) test and the hole-board test (HBT) in rats. For 21 consecutive days, male rats received 50, 100, or 200 mg/kg AAS (i.p.) 30 min prior to a daily injection of CORT. Dysregulation of the HPA axis in response to the repeated CORT injections was confirmed by measuring serum levels of CORT and the expression of corticotrophin-releasing factor (CRF) in the hypothalamus. Daily AAS (200 mg/kg) administration increased open-arm exploration significantly in the EPM test, and it increased the duration of head dipping activity in the HBT. It also blocked the increase in tyrosine hydroxylase (TH) expression in the locus coeruleus (LC) and decreased mRNA expression of brain-derived neurotrophic factor (BDNF) and its receptor, TrkB, in the hippocampus. These results indicated that the administration of AAS prior to high-dose exogenous CORT significantly improved anxiety-like behaviors, which are associated with modification of the central noradrenergic system and with BDNF function in rats. The current finding may improve understanding of the neurobiological mechanisms responsible for changes in emotions induced by repeated administration of high doses of CORT or by elevated levels of hormones associated with chronic stress. Thus, AAS did exhibit an anxiolytic-like effects in animal models of anxiety.

• Biomolecules & Therapeutics
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• 제21권4호
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• pp.313-322
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• 2013

Previous studies have demonstrated that repeated administration of the exogenous stress hormone corticosterone (CORT) induces dysregulation in the hypothalamic-pituitary-adrenal (HPA) axis and results in depression and anxiety. The current study sought to verify the impact of catechin (CTN) administration on chronic CORT-induced behavioral alterations using the forced swimming test (FST) and the elevated plus maze (EPM) test. Additionally, the effects of CTN on central noradrenergic systems were examined by observing changes in neuronal tyrosine hydroxylase (TH) immunoreactivity in rat brains. Male rats received 10, 20, or 40 mg/kg CTN (i.p.) 1 h prior to a daily injection of CORT for 21 consecutive days. The activation of the HPA axis in response to the repeated CORT injections was confirmed by measuring serum levels of CORT and the expression of corticotrophin-releasing factor (CRF) in the hypothalamus. Daily CTN administration significantly decreased immobility in the FST, increased open-arm exploration in the EPM test, and significantly blocked increases of TH expression in the locus coeruleus (LC). It also significantly enhanced the total number of line crossing in the open-field test (OFT), while individual differences in locomotor activities between experimental groups were not observed in the OFT. Taken together, these findings indicate that the administration of CTN prior to high-dose exogenous CORT significantly improves helpless behaviors, possibly by modulating the central noradrenergic system in rats. Therefore, CTN may be a useful agent for the treatment or alleviation of the complex symptoms associated with depression and anxiety disorders.