• Title/Summary/Keyword: SUC2

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Cell Biological Studies on the Mechanism of Development and Differentiation Ⅷ 3. Effects of Polyamines on the Activities of Corn Glucose-6-Phosphate Dehydrogenase, 6-Phosphogluconate Dehydrogenase, and Protein Kinase (생체발생 및 분화기구의 세포생물학적 연구 Ⅷ 3. Polyamine이 옥수수 배의 Glucose-6-Phosphate Dehydrogenase, 6-Phosphogluconate Dehydrogenase 및 Protein Kinase 활성에 미치는 영향)

  • Cho, Young Dong
    • Journal of Plant Biology
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    • v.27 no.2
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    • pp.73-80
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    • 1984
  • Palmitoyl CoA was found to inhibit corn embryo axis glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, which were also inhibites by polyamines. However, reversal of inhibition of both enzymes by palmitoyl CoA was made by spermine. Activity of corn embryo axis protein kinase was found to increase steadily after germination. Activation and inhibition of protein kinase were made by MgCl$_2$and all polymines, respectively. Suc results suggest that fatty acid biosynthesis and lypolysis could be regulated to some extent by polyamines in corn embryo axis.

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Functional Dyeing and Finishing Using Catechins Extracted from Green Tea -Dyeing Optimization and Fastness- (녹차추출 카테킨을 활용한 기능성 염색가공 -염색조건 최적화 및 견뢰도 분석-)

  • Son, Song-I;Jang, Kyung-Jin;Kim, Tae-Kyeong;Jeong, Jong-Suc;Choi, Young-Hee
    • Fashion & Textile Research Journal
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    • v.11 no.2
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    • pp.344-349
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    • 2009
  • Optimum dyeing conditions of green tea extracts were investigated toward nylon, cotton, rayon, and tencel fabrics. Affinity of green tea extracts was exhibited much higher onto nylon fabric than the other cellulosics. As for nylon, the adsorption was increased with the increase of dyeing temperature and optimum dyeing pH was around 4~6. Buildup property of green tea extract was good showing a linear relationship between concentration of the extracts and color strength of dyed fabrics within experimental range. Color fastnesses were good to excellent in general except to light.

Changes in Functional Properties of Casein by Different Chemical Modifications

  • Kim, Il-Sung;Choi, Jin-Ho;Hong, Jeong-Hwa
    • Preventive Nutrition and Food Science
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    • v.2 no.1
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    • pp.17-22
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    • 1997
  • Casein was chemically modified with acetic, succinic, and maleic anhydride and changes in functional pro-perties were evaluated as affected by the degree of modification. Chemical modification resulted in casein with unique functional properties depending upon the type of anhydrid used and the degree of modification. It was possible to control heat coagulation, calcium precipitability, forming and emulsion capacity and stability. At pH 4.5 heat coagulation was 0% in the case 74.1% acetylated casein; on the contrary, succinylation and maleyation resulted in highly heat sensitive protein. Foaming properties were improved markedly by suc-cinylation and maleylation at pH 4.5. However, emulsifying properties were enhanced only by maleylation.

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Adsorption Study on the Radioactive Liquids by Korean Vermiculite (한국산(韓國産) Vermiculite에 의(依)한 방사성동위원소(放射性同位元素) 흡착연구(吸着硏究))

  • Moon, Suc-Hyong
    • The Korean Journal of Nuclear Medicine
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    • v.7 no.1
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    • pp.51-54
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    • 1973
  • The use of ion-exchange resins for the treatment of radioactive wastes has many advantages, but thes eare rather expensive as compared with the Korean vermiculite. The Korean vermiculite has slightly different chemical constituents from the ones produced in other countries, and its physical properties might be applicable to the management of radioactive waste, in a small nuclear installation. The decontaminating effect of Korean vermiculite for the low-level radioactive liquid was investigated. $^{106}Ru,\;^{90}Sr,\;and\;^{137}Cs$ were utilized for the experiments. The removal rates by Korean vermiculite were calculated for $^{106}Ru,\;^{90}Sr\;and\;^{137}Cs$ and the removal rates increased as the weight of vermiculite in the exchange column increased. The decontaminating constants, $K_d$ of the Korean vermiculite for $^{106}Ru,\;^{90}Sr\;and\;^{137}Cs$ were 2.7, 69.3 and 263ml/g respectively. Through the results of experiments, the application of Korean vermiculite column to the treatment of low-level radioactive waste is quite feasible.

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A Study on the Three Phase Glass Fiber/Nylon 6/Polyproylene Composites (나일론 6과 폴리프로필렌 수지에 유리섬유가 보강된삼상 복합재료에 관한 연구)

  • 서문호
    • The Korean Journal of Rheology
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    • v.10 no.2
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    • pp.57-64
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    • 1998
  • A pultrusion resin impregnation (PRI) die, which has been developed recently in our laboratory, was used to pre-pare various composite system. The continuous fiber reinforced composites of glass fiber/polypropylene(GFPP) and glass fiber/polyamide 6 (GFPA) were first manufactured by means of the PRI die and then cut into chopped pellets of predet-ermined length. These pellets and either virgin or modified thermoplastic resin were melt-mixed by a twin screw extruder to prepare GF/PA/PP and GF/PA/PPMA system. The mechanical properties of these blends were investigated and discussed in terms of their morphological observations. These preliminary results revealed that this new impregnation die could be suc-cessfully applied to produce prepregs suitavle for the final shaping process.

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Radiprotective Effects of S-2-($\omega$-aminoalkylamino) ethyl Derivatives and Their Drug Toxicities (S-2-($\omega$-aminoalkylamino) ethyl 유도체(誘導體)들의 방사선장해방호효과(放射線障害防護效果)와 독성(毒性))

  • Kim, You-Sun;Kim, Suc-Won
    • Journal of Radiation Protection and Research
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    • v.8 no.2
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    • pp.1-7
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    • 1983
  • S-2-($\omega-aminoalkylamino) ethyl dihydrogen phosphorothioates and S-2-($\omega$-aminoalkylamino) ethyl isothiuronium bromides were prepared from easily available starting compounds via convenient synthetic processes. The isothiuronium derivatives showed extreme drug toxicities as compared to that of AET, which seemed to be due to an intramolecular rearrangement of these compounds. The propyl derivative of the phosphorothioate could show better radioprotective effect than those of AET and WR-638, whereas the ethyl derivative of the equivalent drug dose revealed far less protective effect. The correlation between radioprotective effects, drug toxicities, and chemical structures were discussed through infrared spectroscopy.

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An On-Chip Differential Inductor and Its Use to RF VCO for 2 GHz Applications

  • Cho, Je-Kwang;Nah, Kyung-Suc;Park, Byeong-Ha
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.4 no.2
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    • pp.83-87
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    • 2004
  • Phase noise performance and current consumption of Radio Frequency (RF) Voltage-Controlled Oscillator (VCO) are largely dependent on the Quality (Q) factor of inductor-capacitor (LC) tank. Because the Q-factor of LC tank is determined by on-chip spiral inductor, we designed, analyzed, and modeled on-chip differential inductor to enhance differential Q-factor, reduce current consumption and save silicon area. The simulated inductance is 3.3 nH and Q-factor is 15 at 2 GHz. Self-resonance frequency is as high as 13 GHz. To verify its use to RF applications, we designed 2 GHz differential LC VCO. The measurement result of phase noise is -112 dBc/Hz at an offset frequency of 100 kHz from a 2GHz carrier frequency. Tuning range is about 500 MHz (25%), and current consumption varies from 5mA to 8.4 mA using bias control technique. Implemented in $0.35-{\mu}m$ SiGe BiCMOS technology, the VCO occupies $400\;um{\times}800\;um$ of silicon area.

Cloning and Expression of Pseudomonas cepacia catB Gene in Pseudomonas putida

  • Song, Seung-Yeon;Jung, Young-Hee;Lee, Myeong-Sok;Lee, Ki-Sung;Kim, Young-Soo;Kim, Chi-Kyung;Choi, Sang-Ho;Min, Kyung-Hee
    • Journal of Microbiology
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    • v.34 no.4
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    • pp.334-340
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    • 1996
  • The enzyme, cis,cis-muconate lactonizing enzyme has been proposed to play a key role in the $\beta$-ketoadipate pathway of benzoate degradation. A 3.2-kb EcoRI fragment termed as pRSU2, isolated from a Pseudomonas cepacia genomic library was able to complement the catB defective mutant. Several relevant restriction enzyme sites were determined within the cloned fragment. In Pseudomonas putida SUC2 carrying pRSU2, the enzyme activity was relatively higher than those of the induced or partially induced state of wild type P. putida PRS2000. It was probably due to higher expression of P. cepacia catB in P. putida PRS2000. It was probably due to higher expression of P. cepacia catB in P. putida. One possible interpretation of these results is that the catB promoter in P. cepacia is recognized within P. putida, resulting in the almost same expression level.

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Cloning and Expression of a Alkaline Protease from Bacillus clausii I-52 (Bacillus clausii I-52로부터 alkaline protease 유전자의 클로닝 및 발현)

  • Joo, Han-Seung;Choi, Jang Won
    • Journal of agriculture & life science
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    • v.45 no.6
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    • pp.201-212
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    • 2011
  • The alkaline protease gene was cloned from a halo-tolerant alkalophilic Bacillus clausii I-52 isolated from the heavily polluted tidal mud flat of West Sea in Inchon Korea, which produced a strong extracellular alkaline protease (BCAP). Based on the full genome sequence of Bacillus subtilis, PCR primers were designed to allow for the amplification and cloning of the intact pro-BCAP gene including promoter region. The full-length gene consists of 1,143 bp and encodes 381 amino acids, which includes 29 residues of a putative signal peptide and an additional 77-amino-acid propeptide at its N-terminus. The mature BCAP deduced from the nucleotide sequence consists of 275 amino acids with a N-terminal amino acid of Ala, and a relative molecular weight and pI value was 27698.7 Da and 6.3, respectively. The amino acid sequence shares the highest similarity (99%) to the nattokinase precursor from B. subtilis and subtilisin E precursor from B. subtilis BSn5. The substrate specificity indicated that the recombinant BCAP could hydrolyze efficiently the synthetic substrate, N-Suc-Ala-Ala-Pro-Phe-pNA,and did not hydrolyze the substrates with basic amino acids at the P1 site. The recombinant BCAP was strongly inhibited by typical serine protease inhibitor, PMSF, indicating that BCAP is a member of the serine proteases.

재조합 Saccharomyces cerevisiae에서 Inulinase와 Invertase의 발현과 분비에 미치는 배양조건의 영향

  • 남수완;신동하;김연희
    • Microbiology and Biotechnology Letters
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    • v.25 no.3
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    • pp.258-265
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    • 1997
  • The effects of medium pH and culture temperature on the expression and secretion of inulinase and invertase were investigated with recombinant Saccharomyces cerevisiae cells. These cells were obtained by transformation of 2$\mu$-based plasmids pYI10 and pYS10 which contain Kluyveromyces marxianus inulinase gene (INU1A) and S. cerevisiae invertase gene (SUC2), respectively, in the downstream of GAL1 promoter. The expression level and localization of inulinase and invertase were not affected significantly by the initial medium pH: secretion efficiencies of inulinase and invertase into the medium were about 90% and 60%, respectively, in the pH ranges of 4.0 to 6.5. However, the expression and secretion of both enzymes were strongly dependent on the culture temperature. The highest expression (7.7 units/mL) and secretion (6.7 units/mL) of inulinase were observed at 28$\circ$C and 30$\circ$C. As a consequence of decreased localization of inulinase in the periplasmic space, the secretion efficiency increased from 68% at 20$\circ$C, to 95% at 35$\circ$C,. The total expression level and secretion efficiency of invertase increased from 19 units/mL and 55% at 20$\circ$C to 25 units/mL and 68% at 35$\circ$C, respectively. Irrespective of the culture temperature, the invertase activity in the cellular fraction (periplasmic space and cytoplasmic fractions) was kept constant at around 33-45%.

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