• Parasites, Hosts and Diseases
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• v.51 no.3
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• pp.305-311
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• 2013

In this study, we found that Haplorchis taichui, a heterophyid intestinal fluke, is highly prevalent, with heavy worm loads, among riparian people in Saravane and Champasak province, Lao PDR. Fecal specimens were collected from 1,460 people (717 men and 743 women) in 12 riparian (Mekong river) districts and were examined by the Kato-Katz fecal smear technique. The overall helminth egg positive rate was 78.8% and 66.4% in Saravane and Champasak province, respectively. The positive rate for small trematode eggs (STE), which included H. taichui and other heterophyids, Opisthorchis viverrini, and lecithodendriids, was 69.9% and 46.3% in Saravane and Champasak province, respectively. To obtain adult flukes, 30 STE-positive people were treated with 40 mg/kg praziquantel and then purged. Whole diarrheic stools were collected 4-5 times for each person and searched for fluke specimens using a stereomicroscope. Mixed infections with various species of trematodes (H. taichui, Haplorchis pumilio, O. viverrini, Prosthodendrium molenkampi, Centrocestus formosanus, and Echinochasmus japonicus) and a species of cestode (Taenia saginata) were found. However, the worm load was exceptionally high for H. taichui compared with other trematode species, with an average of 21,565 and 12,079 specimens per infected person in Saravane and Champasak province, respectively, followed by H. pumilio (41.9 and 22.5, respectively) and O. viverrini (9.4 and 1.5, respectively). These results show that diverse species of intestinal and liver flukes are prevalent among riparian people in Saravane and Champasak province, Lao PDR, with H. taichui being the exceptionally dominant species.

• BMB Reports
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• v.32 no.2
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• pp.161-167
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• 1999

The role of oxidative stress in the initiation and the complication of diabetes was examined by monitoring blood glucose increase and oxidative DNA damage in rats treated with alloxan or streptozotocin (STZ). Oxidative DNA damage was assessed by quantitating 8-oxo-2'-deoxyguanosine ($oxo^8dG)$ excreted in urine and the $oxo^8dG$ accumulated in pancreas DNA. Both alloxan and STZ treatments resulted in an abrupt increase in blood glucose and significant increases in urinary and pancreatic $oxo^8dG$. Pretreatment of buthionine sulfoximine (BSO), a glutathione-depleting agent, slightly potentiated the increase of blood glucose and urinary $oxo^8dG$ in the alloxan- and STZ-treated rats. Furthermore, the BSO pretreatment caused significant amplification of pancreatic $oxo^8dG$ increase in the rats. On the other hand, pretreatment with 1,10- phenanthroline (o-phen), a chelator of divalent cations, showed different results between alloxan- and STZ-treated rats. The o-phen pretreatment completely blocked diabetes and the increase of $oxo^8dG$ by alloxan treatment, while it potentiated the increase of blood glucose and $oxo^8dG$ by STZ treatment. The results demonstrate that the causative effect of alloxan on diabetes may be the generation of reactive oxygen species through a Fenton type reaction, but that of STZ may not.

• BMB Reports
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• v.37 no.3
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• pp.351-355
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• 2004

Separate protocols are commonly used to prepare plasmid DNA, chromosomal DNA, or total RNA from E. coli cells. Various methods for the rapid preparation of plasmid DNA have been developed previously, but the preparation of the chromosomal DNA and total RNA are usually laborious. We report here a simple, fast, reliable, and cost-effective method to extract total nucleic acids from E. coli by direct lysis of the cells with phenol. Five distinct and sharp bands, which correspond to chromosomal DNA, plasmid DNA, 23S rRNA, 16S rRNA, and a mixture of small RNA, were observed when analyzing the prepared total nucleic acids on a regular 1-2% agarose gel. The simple and high-quality preparation of the total nucleic acids in a singe tube allowed us to rapidly screen the recombinant plasmid, as well as to simultaneously monitor the change of the plasmid copy number and rRNA levels during the growth of E. coli in the liquid medium.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.11
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• pp.1922-1928
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• 2008

The thermal rating of a conductor are maximum continuous current capacity and short time emergency current capacity. The overload operation for a faults have an effect on a conductor lifetime. Its time duration and overload level are limited to facility conditions of transmission lines. The short time emergency current capacity in KOREA observe the KEPCO's DESIGN RULE 1210, but its rules are not included to concept of an allowable short time duration. This papers are described to the calculation concept of short time emergency current capacity considering a time duration and an overload level. And we suggested a operation grouping and its maximum conductor temperature considering facility conditions - conductor lifetime, stability of connection points, conductor height above ground and clearance, in the operating and new T/L.

• Journal of Microbiology and Biotechnology
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• v.13 no.4
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• pp.591-597
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• 2003

Human urokinase kringle domain, sharing homology with angiostatin kringles, has been shown to be an inhibitor of angiogenesis, which can be used for the treatment of cancer, rheumatoid arthritis, psoriasis, and retinopathy. Here, the expression of the kringle domain of urokinase (UK1) as a secreted protein in high levels is reported. UK1 was expressed in the methylotrophic yeast Pichia pastoris GS115 by fusion of the cDNA spanning from Ser47 to Lys135 to the secretion signal sequence of ${\alpha}-factor$ prepro-peptide. In a flask culture, the secreted UK1 reached about 1 g/l level after 120h of methanol induction and was purified to homogeneity by ion-exchange chromatography. Amino-terminal sequencing of the purified UK1 revealed that it was cleaved at the Ste13 signal cleavage site. The molecular mass of UK1 was determined to be 10,297.01 Da. It was also confirmed that the purified UK1 inhibited endothelial cell proliferation stimulated by basic fibroblast growth factor, vascular endothelial growth factor, or epidermal growth factor, in a dose-dependent manner. These results suggest that a P. pastoris sytem can be employed to obtain large amounts of soluble and active UK1.

• Journal of Mechanical Science and Technology
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• v.15 no.12
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• pp.1882-1891
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• 2001

The present paper investigated the effect of ultrasonic vibrations on the melting process of a phase-change material (PCM). Furthermore, the present study considered constant heat flux boundary conditions unlike many of the previous researches adopted constant wall temperature conditions. Therefore, in the present study, modified dimensionless parameters such as Ste* and Ra* were used. Also, general relationships between melting with ultrasonic vibrations and melting without ultrasonic vibrations were established during the melting of PCM. Experimental observations show that the effect of ultrasonic vibrations on heat transfer is very important throughout the melting process. The results of the present study reveal that ultrasonic vibrations accompany the effects like agitation, acoustic streaming, cavitation, and oscillating fluid motion. Such effects are a prime mechanism in the overall melting process when ultrasonic vibrations are applied. They enhance the melting process as much as 2.5 tildes, compared with the result of natural melting. Also, energy can be saved by applying ultrasonic vibrations to the natural melting. In addition, various time-wise dimensionless numbers provide conclusive evidence of the important role of ultrasonic vibrations on the melting phenomena.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.196-200
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• 2002

Compositions of four kinds of products inhibit aging and protect many kinds of disease through eliminating. These compositions inhibit growth of harmful bacteria. such as Bacteroides fragilis. Clostridium perfringens. Staphylococcus aureus, Listeria monocytogenes. Vibrio parahaemolyticus etc. Bacteroides fragilis was controlled by mixing SCUTELLARIA BAICALENSIS GEORGE+CRATAEGI FRUCTUS+PAEONIA JAPONICA+SCHIZANDRAE FRUCTUS. SCUTELLARIA BAICALENSIS GEORGE+CRATAEGI FRUCTUS+PAEONIA JAPONICA+CORNI FRUCTUS. etc. and Clostridium perfringens was SCUTELLARIA BAICALENSIS GEORGE+PHELLODENDRI CORTEX+EPIMEDII HERBA+ASTRAGALI RADIX. SCUTELLARIA BAICALENSIS GEORGE+EPIMEDII HERBA +ASTRAGALI RADIX+GLYCYRRHIZAE RADIX.ste. Listeria monocytogenes was NELUMBO NUCLFERA GAERTNER+SCUTELLARIA BAICALENSIS GEORGE+COPTIDIS RHIZOMA+PAEONIA JAPONICA, NELUMBO NUCLFERA GAERTNER+SCUTELLARIA BAICALENSIS GEORGE+COPTIDIS RHIZOMAtSOPHORAE FLOS. etc. and Vibrio parahaemolyticus was ACANTHODANACIS CORTEX +ASTRAGALI RADIX+PHELLODENDRI CORTEX+COPTIDIS RHIZOMA ACANTHODANACIS CORTEX +ASTRAGALI RADIX + PHELLODENDRI CORTEX+PAEONIA JAPONICA. We expected it as a functional food economical and easy type of taking.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.44 no.3
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• pp.256-265
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• 2016

Software based fault tolerance techniques are designed to allow a system to tolerate software faults in the system. Fault tolerance techniques are divided into two groups : software based fault tolerance techniques and hardware based fault tolerance techniques. We need a proper design method according to characteristics of the system. In this paper, the concepts of software based fault tolerance techniques for Dual Flight Control Computer are described. For software based fault tolerance design, we classified software failure, designed a way for failure detection and the way of recovery. Eventually the effectiveness of software based fault tolerance techniques was verified through the Software Test Environment(STE).

• Journal of architectural history
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• v.14 no.4 s.44
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• pp.157-175
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• 2005

Guarino Guarini(1624-1683) is one of great Baroque architects who developed new spatial concept in architecture. He refused to static space typically appeared in Renaissance architecture. Instead, to make it possible to generate complicate form and moving space, he made use of generative diagram. It provide him with an abstract machine to generate automatically architectural inferiority. His generative diagram consists of three types: single circle diagram, matrix diagram and longitudinal diagram. The first diagram uses single circle as primary generator and develop this by means of overlap and equiangular division. La Cappella della Santissima Sindone, Sanctuary of Chiesa di Oropa, Chiesa dei Padri Somaschi, San Gaetano are designed according to this diagram. The generator of the second diagram is nine circles in $3{\times}3$ matrix, which provide the base for the interpenetration of space in Guarini's architecture. He inspired this diagram from Kepler's $\ulcorner$Harmonices mundi$\lrcorner$. The Churches of San Lorenzo, Ste-Anne-la-Royale, San Filippo Neri, San Gaetano are generated by this diagram. The third diagram has several circles in Lantin-cross plan. Guarini adopted this diagram because he had chances to design several churches in Northern Europe. The churches of Santa Maria di Ettinga, Immacolata Concezione, San Maria della Divina Providenza, Church without Name, San Filippo Neri are representative examples for this diagram.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2009.05a
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• pp.336-339
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• 2009

In this paper, such as battery power or solar energy and fuel cells generated from Renewable energy sources, high voltage to low voltage DC-DC Converter for converting the design of the study. System consists of low voltage ($24{\sim}28$ [VDC]) and Boosts the voltage (270 [VDC]) for a 3 [kW] DC-DC converter and control circuit is configured as, Power switch the ST Tomson's Automotive low voltage high current MOSFET switches STE250NS10S (temperature 250A) was applied to the two parallel. Also, Controller's processor used ATMEGA128, and Gate Drive applies and composed Photo Coupler TLP250. development. Input voltage (24V) and output voltage (270V) for Conversion in the H-bridge converter topology of the circuit output side power and voltage to control the implementation of the Phase shift angle control applied. And, 3kW of power to pass appropriate specification of the secondary side as interpreted by the high frequency transformer, and the experimental production and analysis of the experiment