• Korean Journal of Veterinary Service
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• v.35 no.2
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• pp.99-104
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• 2012

Otitis externa (OE) is a frequent disease in the ear canals of dogs. To identify the pathogens causing OE in dogs and to determine their antimicrobial resistances, specimens were collected from animal hospitals in Daejeon. The isolates were examined by morphological and biochemical tests, 16S rRNA analysis and antimicrobial susceptibility tests. We analyzed correlation between the isolated pathogens and external factors of dogs such as breed, age, gender, ear mite, hair in ears and experience with antibiotic therapy. Thirty three strains of bacteria were isolated from 26 of the 68 heads of dogs with OE. The most isolated bacteria were Enterococcus faecalis (E. faecalis) followed by Staphylococcus aureus (Sta. aureus), Sta. pseudointermedius, E. faecium, E. avium and Streptococcus canis (Strep. canis) in order of frequency of occurrence. Isolation frequency of Enterococcus spp. and Staphylococcus spp. were 51.5% and 45.5%, respectively. E. faecalis and E. faecium isolates showed VanB phenotype, which is resistant to vancomycin but sensitive to teicoplanin were 58% and 25%, respectively. Nine isolates among total twelve isolates of E. faecalis were isolated from the dogs treated with antibiotics. There was no methicillin-resistant Sta. aureus (MRSA), but were MR-Sta. pseudointermedius (MRSP) (57.1%) and vancomycin-resistant (VR)-Sta. pseudointermedius (14.3%) (VRSP) showing VanB phenotype. However, vanA, vanB and vanC genes were not detected in VR isolates from the dogs. Taken together, VR-Enterococcus spp. (VRE) is one of the major pathogens in domestic animals, as well as community-and hospital-acquired infection.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.2
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• pp.248-251
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• 1983

The effects of shading - 90, 70, 50, 30, 10% on curing burley tobacco was investigated in pipe vinyl houses. The curing in sunlight under transparent materials resulted in an increase in temperature and a decrease in relative humidity. But amount of light on tobacco leaves were less than 10% sun light in all treatments. Shading was delayed curing period, but cured leaves under transparent shading materials were off color and brittle. Filling power and combustibility were deteriorated as decreased shading. But quality of cured leaves were non significant between treatments. Effects of shading were believed that resulted in a decrease in temperature and an increase in relative humidity than light.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.411-411
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• 2021

최근 들어 이상기후에 따른 집중호우의 영향과 하천 유역 내에서의 토지이용의 증가로 하천의 하류부에서의 홍수피해는 점점 증가하고 있다. 이에 대비하기 위해 하천 시설물 설치를 계획하거나 이수, 치수 계획을 세우기 위해서는 하상이 어떻게 변화할 것인지 파악하는 것이 중요하다. 연구 대상 지역 세종특별자치시 금남면 영대리 유천천은 최근 10년간 홍수로 인한 피해액이 연평균 약 2.4억 원으로 나타났으며 매년 피해가 지속되고 있다. 유천천은 인위적 하도의 변경과 토지이용 상황의 변화 및 하천 골재채취, 댐 및 저수지 건설, 자연적인 홍수 등으로 하상이 변동되고 있으며, 그로 인해 생긴 유로의 변화로 발생할 수 있는 피해 예측이나 하천 시설물 설치를 위해서는 변동 결과의 예측이 필요하다. 따라서 본 연구에서는 대상 유역의 하상변동을 분석하기 위하여 HEC-RAS Sediment Transport Analysis(STA) 모형을 적용하였다. 적용한 데이터를 바탕으로 장래 하상변동 결과를 예측하였고 GIS 기법을 이용하여 유역의 특성 및 유출 특성에 관한 인자들을 도출하였다. 장래 1년, 5년, 10년 단위로 하상변동의 예측이 가능한 HEC-RAS STA 모형 분석 결과, 하상경사가 급한 각 하천상류 구간과 횡단시설물의 상류층에서는 하상저하가 발생하고, 하상경사가 완만한 하류 구간 부근에서는 일부 하상 상승이 발생하는 것으로 나타났다. 수위상승에 대비하여 지속적인 관찰과 상승구간에 대한 주기적인 하천 정비를 통하여 홍수기에 수위상승을 억제할 필요가 있을 것으로 판단된다. 본 연구의 결과는 하도 안정성과 이수와 치수안정성 확보, 하천의 효율적인 이용과 보전, 유지관리 시 기본해석으로 활용할 수 있을 것으로 사료된다.

• Archives of Plastic Surgery
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• v.32 no.2
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• pp.214-218
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• 2005

The anatomy and distribution of the superficial temporal vessels are known to follow regular patterns, with few exceptions in previous studies. But these previous studies of the anatomy and distribution of the superficial temporal vessels were based, only on the cadaver studies. Authors evaluated the anatomy of these vessels in the operative field of the living body from December 1997 to June 2001, The superficial temporal vessels were surgically exposed from the zygomatic arch extending to the superior temporal line through a preauricular incision in 18 patients(20 cases), who underwent reconstructive surgery using these vessels in the operative field. The authors measured and analyzed; the distribution, branching and diameters of the superficial temporal vessels. The results were obtained as follows; 1.In 19 cases, STV(superficial temporal vein) runs posterior to STA(superficial temporal artery) at the upper border of the zygomatic arch. 2. There was no frontal or parietal branches in 2 cases and vena comitante existed along with STA in one case. 3. The external diameter of STA and STV was measured at the lower border of the zygomatic arch. The external diameter of STA ranged from 1.5 mm to 3.0 mm(average 2.1 mm) and those of STV ranged from 1.3 mm to 3.5 mm (average 2.0 mm). This study can be helpful in the reconstructive surgery using the superficial temporal vessels, for the results of our study are based on the true anatomy of the living body.

• Korean Journal of Veterinary Research
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• v.40 no.2
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• pp.301-310
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• 2000

The antimicrobial susceptibility, genotypes of enterotoxins(LT, STa) and pili(K88, 987P), and plasmid profiles were investigated with 102 Escherichia coli isolated from piglets showing diarrhea in Korea. Almost of them were susceptible to ceftiofur(99%), cefquinone(97.1%). However they showed resistance to bacitracin(100%), streptomycin(98%), vancomycin(97%), trimethoprim/sulfamethoxazole(87.2%), tetracycline(84.3%) in antimicrobial susceptibility test. Moreover, all of the isolates demonstrated resistance to more than 2, and 78% of them were resistant to more than 8 of total 17 drugs. Multiplex PCRs for genotyping of enterotoxins(LT, STa) and pili(K88, 987P) were established with primers designed based on sequences from Genebank. Seventeen strains(16.6%) of the isolates had STa gene, 11 strains(10.8%) of them had both STa and LT genes, and 18 strains(17.8%) had K88 gene. But none of the isolates harbored a gene exclusively encoding LT. The gene encoding 987P pili was not found in all isolates. Fifty-four strains of 102 isolates(52.9%) had plasmid with various sizes ranging from 125kb to 1.1kb. Numbers of plasmid per isolates were also various, from 1 to 9. Distinctive relationship between plasmid profiles and genotypes of enterotoxins and pili in the isolates was not found. These results might provide the basic knowledge to establish strategies for the treatment and prevention of colibacillosis in piglets.

• Health Policy and Management
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• v.29 no.1
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• pp.86-89
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• 2019

Catastrophic health expenditure refers to spending more than a certain level of household's income on healthcare expenditure. The aim of this study was to investigate the proportion of households that experienced catastrophic health expenditure between 2006 and 2017 with the National Survey of Tax and Benefit (NaSTaB) and between 2011 to 2016 using Households Income and Expenditure Survey (HIES) data. The results of the NaSTaB showed 2.16% of households experienced the catastrophic health expenditure in 2017. In trend analysis, the NaSTaB revealed a statistically significant decreasing trend (annual percentage change [APC] = -2.01, p<0.001) in the proportion of households with the catastrophic health expenditure. On the other hand, the results of the HIES showed 2.92% of households experienced the catastrophic health expenditure in 2016. Also, there was a slightly increasing trend (APC= 1.43, p<0.001). In subgroup analysis, groups with lower income levels were likely to experience catastrophic health expenditure. In conclusion, further public support system is needed to lower experience these healthcare expenditures and monitor the low income group.

• Journal of Bio-Environment Control
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• v.10 no.3
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• pp.141-147
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• 2001

To compare to the optical and physical properties of covering materials for plastic greenhouse, EVA(ethylene vinyl acetate, 0.08 mm), polyorefine antifog (0.1 mm), fluoric (0.06 mm), diffused (0.15 mm), polyorefine antidrop (0.15 mm) and PET (polyethylene terephthalate, 0.5 mm) films were used. The small greenhouse (5.4$\times$18.5$\times$2.9 m, W$\times$L$\times$H) investigated during 3 years form 1997 to 1999. After covering materials were used for greenhouse covering during 30 months, UV (300-400 nm) transmittances of diffused film and PET were appeared from 25 to 26%, while those of fluoric film and the other films were 76% and from 63 to 67%. For PAR (photosynthetically active radiation, 400-700 nm), the transmittances of fluoric, antidrop, PET, antifog, EVA, and diffused film were 86.5%, 80.5%, 76.3%, 75.5%, 74.1% and 61.9% respectively. The losses of PAR transmittance of EVA and the antidrop film during period between 7 days and 30 months were higher value 12% and lower value 6% than any other film. Under the canopy of tomato plants, light intensities of the diffused film and the antifog film were 2.5 times and 1.4 times higher than those of PET. Tensile resistances of fluoric film at the break point were the higher than those of antifog film and diffused film. While impact resistance of the antidrop film was the highest value, but the fluoric film was the lowest. Air temperature inside the greenhouse for the day showed to be changed the similar light transmittance of the films. But the increasing order of air temperature for the night was PET, fluoric, antidrop, diffused, antifog and EVA film. Especially, air temperature in the PET was 4$^{\circ}C$ higher than that in the EVA. Solar radiations of the fluoric film, the antidrop film, PET and antifog film in the greenhouse were 32%, 15%, 11% and 4% higher than those of PET. However, those of the diffused film was 7% less than PET.

• Korean Journal of Veterinary Service
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• v.24 no.1
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• pp.31-41
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• 2001

This study was carried out to identify causative agents from california mastitis test(CMT) positive mastitic milk, and to examine the antimicrobial susceptibility of 50 heads in Seongju and Chilgok area of Gyeongbuk province. Sulfadimethoxine sodium(SMS) was intramuscularly administered once to four mastitis Infected dairy cattle at the rate of 10mg/kg body weight. After injection of SMS, the depletion rate of serum and milk from the cattle were periodically measured for five days. The CMT positive number from 50 heads showed 46% and that of 200 quarters was 47(23.5%). From 39 quarters of 47 heads 39 different microorganisms were identified. These organisms were classified into 12 species : Staphylococcus aureus 8(20.5%), Sta hemolyticus 6(15.4%), Streptococcus bovis 4(10.3%), Sta hyicus 3(7.7%), Sta epidemidis. Sta xylosus, Sta sciuri 2(5.1%), Str agalactiae 2(5.1%), Escherichia coli(10.3%), three Enterobacter cloacae(7.7%), two Ent aerogenes(5.1%) and one Salmonella spp(2.6%). As the results of antibiotic susceptibility test, gentamicin(Gm, 11 species 27 strains, 69.5%), cephalotin(Cf, 9 species 24 strains, 61.5%), sulfamethoxazole(Stx, 8 species 14 strains, 43.6%), tetracycline(Tc, 8 species 14 strains, 35.9%), and streptomycin(Sm), lincomycin(Lm), cefoperazon(Cp) and penicillin(Pc) have a highly resistance(7.7% ～5.1%). However, carbenicillin (Cb), amikacin(Ah) have no susceptible for all drugs. The mean residual concentration of SMS in serum according to the time lapsed were showed 33.964 $\pm$ 4.435ppm at the 4 hours after intramuscularly injection(AII). It was significantly(p<.05) decreased to 6.596 $\pm$ 3.402, 0.217 $\pm$ 0.119 and 0.005 $\pm$ 0.004ppm at the 1st, 3rd and 5th day AII. The mean concentration of SMS in milk was significantly(p<.05) decreased from 0.920 $\pm$ 0.42ppm to 0.084 $\pm$ 0.016ppm between 8 hours and 1 day AII. As the results of this experiments, sulfadimethoxine was residued at the level of no less than 0.01ppm in milk on the 2nd day AII. Thus, this results would be able to be used the basic index for prevention of sulfonamides residue in milk after treatment of dairy mastitis.

• YAKHAK HOEJI
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• v.55 no.2
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• pp.106-115
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• 2011

Systematic toxicological analysis (STA) means the process for general unknown screening of drugs and toxic compounds in biological fluids. In order to establish STA, in previous study we investigated pattern of drugs & poisons in autopsy cases during 2007~2009 in Korea, and finally selected 62 drugs as target drugs for STA. In this study, rapid and simple drug identification and quantitative analytical program by gas chromatography-mass spectrometry(GC-MS) was developed. The in-house program, "DrugMan", consisted of modified chemstation data analysis menu and newly developed macro modules. Total 55 drugs among 62 target drugs were applied to this program, they were 14 antidepressants, 8 anti-histamines, 5 sedatives/hypnotics, 5 narcotic analgesics, 3 antipsychotic drugs, and etc. For calibration curves, fifty five drugs were divided into four groups of range considering their therapeutic or toxic concentrations in blood specimen, i.e. 0.05~1 mg/l, 0.1~1 mg/l, 0.1~5 mg/l or 0.5~10 mg/l. Standards spiked bloods were extracted by solid-phase extraction (SPE) with trimipramine-D3 as internal standard. Parameters such as retention times, 3 mass fragment ions, and calibration curves for each drug were registered to DrugMan. A series of identification, semi quantitation of target drugs and reporting the results were performed automatically. Calibration curves for most drugs were linear with correlation coefficients exceeding 0.98. Sensitivity rate of DrugMan was 0.90 (90%) for 55 drugs at the level of 0.5 mg/l. For standard spiked bloods at the level of 0.5 mg/l for 29 drugs, semi quantitative concentrations were ranged 0.36~0.64 mg/l by DrugMan. If more drugs are registered to database in DrugMan in further study, it will be useful tools for STA in forensic toxicology.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.476-482
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• 2001

This experiment was conducted to evaluate the effect of slow-release fertilizer application on quality elevation of onion (Allium cepa L.) in southwestern sea. The slow-release fertilizers used were NIAST (National Institute of Agricultural Science and Technology) I, II, CDU (Crotonylidene Diurea), UF (Urea-Formaldehydes), and IBDU (Isobutylidene Diurea) 30, 50. Growth of the onion plant treated with slow-release fertilizer showed better than the control. However, a rate of infected plant to downy mildew was low as compared with control. Chlorophyll and total nitrogen contents were high in control, whereas firmness, soluble solid content, number and thickness of scaly leaves were high in slow-release fertilizer plot. In early maturing onion, total marketable bulb yield was higher in slow-release fertilizer plot than all other fertilizer treatments. Total marketable bulb yield in late maturing onion treated with slow-release fertilizer was slightly decreased. Decaying loss and the rate of sprouting during storage period decreased in slow-release fertilizer application. In summary, application of slow-release fertilizer increased marketable yield, storability after harvest and also reduced labor needs for top dressing.